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研究生:陳旻佐
論文名稱:石蓴及石蓴酒粕生物活性探討
論文名稱(外文):Evaluation on the bioactivity of Ulva lactuca and Ulva wine spent
指導教授:蔡國珍
指導教授(外文):Guo-Jane Tsai
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:食品科學系
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:89
中文關鍵詞:酒粕石蓴酒粕
外文關鍵詞:wine spentUlva wine spent
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摘要
本研究以熱水、 50%甲醇及 50%乙醇萃取石蓴粉末及石蓴酒粕,分析各萃取物之抗氧化能力、抗老化及美白能力。各石蓴酒粕萃取物之總酚含量均大於石蓴萃取物,以石蓴酒粕 50%乙醇萃取物總酚含量最高 18.39 ± 1.30 �慊/mg。石蓴粉末各萃取物類黃酮含量均大於石蓴酒粕各萃取物,以石蓴粉末 50%乙醇萃取物含有最高類黃酮含量 8.50 ± 2.28 �慊/mg。石蓴酒粕萃取物之還原力、 ABTS清除率及清除 DPPH能力均大於石蓴粉末萃取物,以 50%乙醇萃取物有較高的活性,還原力相當於13.20 ± 1.01 �慊/mg Vit C;當其濃度為 10 mg/mL之清除ABTS及 DPPH能力分別為 61.15 ± 0.17%及 99.85 ± 0.2%。石蓴粉末萃取物螯合亞鐵離子能力高於石蓴酒粕萃取物,以 50%乙醇萃取物為最佳,當其濃度為 10 mg/mL之螯合能力為 84.82 ± 4.47%。石蓴粉末各萃取物對於人類皮膚纖維母細胞 CCD-966SK增生的情形較為顯著, 1000 �慊/mL之 50%乙醇萃取物培養細胞,細胞增生率為 149.81 ± 5.49%。濃度 500 �慊/mL 石蓴粉末及酒粕之熱水萃取物,可使 CCD-966SK分泌膠原蛋白量從控制組之14.01 ± 0.54 �慊/mL,分別增加至 38.30 ± 8.09 �慊/mL及 22.00 ± 4.95 �慊/mL。石蓴粉末及酒粕熱水及 50%乙醇萃取物濃度大於 500 �慊/mL時,可修護紫外線對 CCD-966SK之傷害而增加其存活率。人類黑色瘤細胞 A375經 1000 �慊/mL石蓴粉末之 50%乙醇及熱水萃取物處理之存活率,分別為 45.71%及 64.72%;同濃度之石蓴酒粕之此二種萃取物,所得存活率分別為 64.63%及 89.71%。可能與此等萃取物於濃度 500 �慊/mL時,皆能誘導 A375胞內活性氧物質生成有關。石蓴酒粕之酪胺酸?抑制活性優於石蓴粉末,以石蓴酒粕 50%乙醇萃取物有著最好的抑制率 45.58 ± 2.35%。
目錄
壹、前言 1
貳、文獻整理 3
一、石蓴 3
1.1一般成分 3
1.2抗微生物活性 4
1.3抗發炎 5
1.4降血脂 5
1.5抗氧化 6
1.6 抗凝血 7
1.7其他功能 7
二、石蓴酒及酒粕 8
2.1 石蓴酒之釀造 8
2.2 石蓴酒之儲酒條件及對大鼠抗氧化活性 8
2.3 酒粕簡介及用途 9
2.3.1 生質能源 10
2.3.2 生產蛋白?及蛋白酮 10
2.3.3 飼料 11
2.3.4 美容 11
三、皮膚之構造與黑色素 12
3.1 皮膚構造 12
3.1.1 表皮 12
3.1.2 真皮 13
3.1.3 皮下組織 13
3.2 黑色素之簡介 14
3.2.1 黑色素細胞 14
3.2.2 黑色素簡介及生成 14
3.2.3 黑色素的功能 15
3.2.4 黑色素與膚色的關係 16
3.3 酪胺酸? 16
四、皮膚之美白 17
4.1 還原黑色素 17
4.2 抑制酪胺酸?活性 18
4.3 與反應的中間物形成穩定的化合物 19
4.4 促進表皮內的黑色素排除 19
4.5 阻斷 SCF/KIT訊號傳遞 19
五、皮膚之抗老化 20
5.1 皮膚老化之概要 20
5.1.1 自然老化 20
5.1.2 光老化 21
5.2 皮膚之抗老化 23
參、實驗設計 25
肆、材料與方法 26
一、材料 26
1. 釀酒原料 26
2. 菌株與細胞株來源 26
2.1 菌株 26
2.2 細胞株 26
3、培養基 26
4、化學藥品 27
4.1 成份分析 27
4.2 抗氧化 27
4.2.1 總酚含量分析 27
4.2.2 類黃酮含量分析 27
4.2.4 還原力分析 27
4.2.3 DPPH 自由基清除能力 28
4.2.5 Trolox抗氧化當量活性測定 28
4-2.6 螯合亞鐵離子能力 28
4.3 抗老化試驗 28
4.3.1 細胞存活率之測定 28
4.3.2萃取物對膠原蛋白合成之影響 29
4.3.3抗紫外線能力試驗 29
4.4 美白試驗 29
4.4.1 萃取物對於酪胺酸?活性的影響 29
4.4.2 萃取物對黑色素細胞生成 ROS之影響 29
5、儀器設備 29
二、實驗方法 31
1、石蓴酒釀造 31
1.1 石蓴前處理 31
1.2 菌株保存 31
1.3 菌株活化 31
1.4 發酵酒液及酒粕收集 31
2、石蓴粉原料及酒粕萃取 32
2.1 熱水萃取 32
2.2 乙醇萃取 32
2.3 甲醇萃取 32
3、石蓴酒粕一般成份分析 33
3.1 水分測定 33
3.2 灰份測定 33
3.3 粗蛋白分析 33
3.4 粗脂肪分析 34
3.5 碳水化合物分析 34
4、抗氧化活性測定 35
4.1 總酚含量分析 35
4.2 類黃酮含量分析 35
4.3還原力測試 35
4.4 DPPH 自由基清除能力 36
4.5 Trolox抗氧化當量活性測定 36
4.6螯合亞鐵離子能力 36
5、細胞培養 37
5.1 人類皮膚纖維母細胞 CCD-966SK與黑色素瘤 A375細胞培養 37
5.2 細胞繼代培養 37
5.3 細胞計數 37
6、抗老化試驗 38
6.1 萃取物對人類皮膚纖維母細胞 CCD-966SK生長之影響 38
6.2 萃取物對膠原蛋白合成之影響 38
6.3、抗紫外線能力試驗 39
6.3.1 人類皮膚纖維母細胞 CCD-966SK經紫外線照射後之存活率 39
6.3.2 萃取物對於人類皮膚纖維母細胞 CCD-966SK抗紫外線能力之影響 40
7、美白試驗 40
7.1 酪胺酸?活性抑制 40
7.2萃取物對黑色素細胞存活率之影響 41
7.3萃取物對於黑色素細胞生成 ROS之影響 41
8. 統計分析 41
伍、結果與討論 42
一、一般成分 42
二、萃取率 42
三、石蓴粉末及酒粕之抗氧化活性 43
3.1 總酚與類黃酮含量 43
3.3 還原力 44
3.2 清除DPPH之能力 45
3.5 清除 ABTS之能力 46
3.4螯合亞鐵離子能力 47
四、抗老化試驗 48
4.1 萃取物對人類皮膚纖維母細胞 CCD-966SK增生效果 48
4.2 萃取物對人類皮膚纖維母細胞 CCD-966SK膠原蛋白合成之影響 50
4.3 人類皮膚纖維母細胞 CCD-966SK經紫外線照射後之存活率 51
4.4萃取物對於經紫外線照射後人類皮膚纖維母細胞 CCD-966SK之存活率 51
五、美白試驗 54
5.1 酪胺酸?活性抑制 54
5.2 萃取物對人類黑色素瘤細胞 A375生長之影響 55
5.3 萃取物對人類黑色素瘤細胞 A375生成 ROS之影響 56
陸、結論 58
柒、參考文獻 59

圖表目錄
圖一、石蓴粉末 (A) 及酒粕 (B) 不同溶劑萃取物之還原力。 66
圖二、石蓴粉末 (A) 及酒粕 (B) 不同溶劑萃取物之 DPPH清除率。 67
圖三、石蓴粉末 (A) 及酒粕 (B) 不同溶劑萃取物之 ABTS清除率。 68
圖四、石蓴粉末 (A) 及酒粕 (B) 不同溶劑萃取物之螯合亞鐵能力。 69
圖五、人類皮膚纖維母細胞 CCD-966SK經石蓴粉末萃取物處理不同時間對其生長之影響。 70
圖六、人類皮膚纖維母細胞 CCD-966SK經石蓴酒粕萃取物處理不同時間對其生長之影響。 71
圖七、人類皮膚纖維母細胞 CCD-966SK經紫外線 A照射不同時間之存活率 72
圖八、石蓴粉末熱水萃取物 (A) 及 50%乙醇萃取物 (B) 對人類皮膚纖維母細胞 CCD-966SK照射紫外線 UVA存活率之影響。 73
圖九、石蓴酒粕熱水萃取物 (A) 及 50%乙醇萃取物 (B) 對人類皮膚纖維母細胞 CCD-966SK照射紫外線 UVA存活率之影響。 74
圖十、石蓴粉末 (A) 及酒粕 (B) 之萃取物對酪胺酸?之抑制率。 75
圖十一、石蓴粉末 (A) 及酒粕 (B) 不同溶劑萃取物對於人類黑色素瘤細胞 A375存活率之影響。 76
圖十二、石蓴粉末熱水與 50%乙醇萃取物對人類黑色素瘤細胞 A375生成胞內 ROS之影響。 77
圖十三、石蓴酒粕熱水與 50%乙醇萃取物對人類黑色素瘤細胞 A375生成胞內 ROS之影響。 78
表一、石蓴粉末及酒粕之乾重組成份…………………………………………………..79
表二、石蓴粉末及酒粕不同溶劑萃取物之萃取率 79
表三、石蓴粉末及其不同溶劑之萃取物總酚及類黃酮含量 80
表四、石蓴酒粕及不同溶劑萃取物之總酚及類黃酮含量 80
表五、不同萃取方法而得石蓴粉末之清除DPPH、螯合亞鐵能力、還原力及清除 ABTS能力 81
表六、不同萃取方法而得石蓴酒粕之清除DPPH、螯合亞鐵能力、還原力及清除 ABTS能力 82
表七、石蓴粉末及酒粕之熱水與 50%乙醇萃取物對於 CCD-966SK膠原蛋白生合成之影響 83
壹、
柒、參考文獻
日本健康營養食品協會。健康食品規格基準 公示。平成 7年 6月1日。
方銘志。2002。山藥多醣體之免疫調節機能探討,國立台灣海洋大學水產食品科學研究所碩士論文。基隆。台灣。
陳宜嫻。2001。皮膚黑色素抑制劑與保濕劑效能之研究,靜宜大學應用化學研究所博士論文。台中。台灣。
張麗卿。2007。現代化�菻~新論。高立圖書有限公司。台北。台灣。
黃美勳。1996。中藥對酪胺酸?氧化作用之活性研究,中國醫藥學院中國藥學研究所。台中。台灣。
黃淑芳。2000。臺灣東北角海藻圖錄。臺灣博物館。臺北。台灣。 pp. 23-58。
廖國威。2007。石蓴酒貯酒條件及對大鼠抗氧化活性探討。國立臺灣海洋大學食品科學系碩士學位論文。基隆。台灣。
劉育綾。2005。石蓴酒的釀造及其抗氧化活性分析。國立臺灣海洋大學食品科學系碩士學位論文。基隆。台灣。
蘇娟娟。2003。米酒糟蛋白質對人類真皮層纖維母細胞生長的影響。花蓮。台灣。
Alonso-Lebrero, J. L., Dominguez-Jimenez, C., Tejedor. R., Brieva, A., Pivel, J. P. 2003. Photoprotective properties of a hydrophilic extract of the fern Polypodium leucotomos on human skin cells. Journal of Photochemistry and Photobiology, 70, 31-37.
Andrawis, A., Kahn, V., 1986. Effect of methinazole on the activity of mushroom tyrosinase. Biochemical Journal, 235, 91-96.
AOAC. 1995. Official methods of analysis. Association of oddi cal analytical chemists. Washington, DC, USA.
Athukorala,Y., Kim, K. N. Jeon, Y. J., 2006. Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food and Chemical Toxicology, 44, 1065-1074.
Awad, N.E., 2000. Biologically active steroid from the green alga Ulva lactuca. Phytotherapy Research, 14, 641-643.
Buszman, E., Wrzesniok, D., Surazynski, A., Palka, J., Moled, K., 2006. Effect of melanin on netilmicin-induced inhibition of collagen biosynthesis in human skin fibroblasts. Bioorganic and Medicinal Chemistry, 14, 8155-8161.
Campisi, J., 1996. Replicative senescence: an old wives tale. Cell, 84, 497-500.
Castro, R., Zarra, I., Lamas, J., 2004. Water-soluble seaweed extracts modulate the respiratory burst activity of turbot phagocytes. Aquaculture, 229, 67-78.
Chen, Q., Ames, B.N., 1994. Senescence-like growth arrest induced by hydrogen peroxide in human diploid fibroblast F65 cells. Proceedings of the National Academy of Sciences, 91, 4130-4134.
Chew,Y. L., Lim, Y.Y., Omar, M., Khoo, K.S., 2007. Antioxidant activity of three edible seaweeds from two areas in South East Asia. Food Science and Technology, 10,1016-1022
Cho, S., Lowe, L., Hamilton, T. A., Fisher, G. J., Voorhees, J. J., Kang, S., 2005. Long-term treatment of photoaged human skin with topical retinoic acid improves epidermal cell atypia and thickens the collagen band in papillary dermis. American Academy of Dermatology, 53, 769-774.
Choi, H. K., Lim, Y. S., Kim, Y. S., Park, S. Y., Lee, C. H., Hwang, K. W., Kwon, D. Y., 2008. Free-radical-scavenging and tyrosinase-inhibition activities of Cheonggukjang samples fermented for various times. Food Chemistry, 106, 564–568.
Dien, B. S., Ximenes, E. A., O’Bryan, P. J., Moniruzzaman, M., Li, X. L., Balan, V., Dale, B., Cotta M. A., 2008. Enzyme characterization for hydrolysis of AFEX and liquid hot-water pretreated distillers’ grains and their conversion to ethanol. Bioresource Technology, 99, 5216-5225.
Fisher, G.J., Talwar, H.S., Lin, J., Lin, P., McPhillips, F., Wang, Z., Li, X., Wan, Y., Kang, S., Voorhees, J.J., 1998. Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occurs subsequent to activation of mitogen- activated protein kinase pathways in human skin in vivo. Journal of Clinical Investigation, 101, 1432-1440.
Fleurence, J., 1999. Seaweed proteins :biochemical,nutritional aspects and potential uses. Trends in Food Science and Technology, 10, 25-28.
Gilchrest, B.A., 1989. Skin ageing and photoageing. An overview. Journal of the American Academy of Dermatology, 21, 510-513.
Grichnik, J. M., Burch, J. A., Burchette, J., Shea, C. R., 1998. The SCF/KIT pathway plays a critical role in the control of normal human melanocyte homeostasis. Journal of Investigative Dermatology, 111, 233-238.
Gupta, S. K., 2007. Novel hydroxy acid complexes for antiaging and skin renovation. Bioderm Research, 11, 309-441.
Godard, M., Decorde, K., Ventura, E., Soteras, G., Baccou, J., Cristol, J., Rouanet, J., 2009. Polysaccharides from the green alga Ulva rigida improve the antioxidant status and prevent fatty streak lesions in the high cholesterol fed hamster, an animal model of nutritionally-induced atherosclerosis. Food Chemistry, 115, 176-180.
Hahhiew, I. M. Eller, M. S. Gilchrest, B. A., 2000. Skin aging and photoaging: The role of DNA damage and repair. Anwricatt Journal of C’otttacl Dertnalitis, 11, 19-25.
Haywood, R., Rogge, F., Lee M., 2008. Protein, lipid, and DNA radicals to measure skin UVA damage and modulation by melanin. Free Radical Biology and Medicine, 44, 990-1000.
Herrling, T., Jung, K., Fuchs, J., 2008. The role of melanin as protector against free radicals in skin and its role as free radical indicator in hair. Spectrochimica Acta Part A, 69, 1429-1435.
Ito, s., Wakamatsu, K., 2003. Quantitative analysis of eumelanin and pheomelanin in humans, mice, and other animals: a comparative review. Pigment Cell Research, 16, 523-531.
Jenkins, G., 2002. Molecular mechanisms of skin ageing. Mechanisms of Ageing and Development, 123, 801-810.
Jin, D.Q., Lim, C.S., Sung, J.Y., Choi, H.G., Ha, I., Han, J.S., 2006. Ulva conglobata, a marine algae, has neuroprotective and anti-inflammatory effects in murine hippocampal and microglial cells. Neuroscience Letters, 402, 154-158.
Karioti, A., Protopappa, A., Megoulas, N., Skaltsa, H., 2007. Identification of tyrosinase inhibitors from marrubium velutinum and marrubium cylleneum. Bioorganic and Medicinal Chemistry, 15, 2708-2714.
Kblagatian, M., Hadshiew, I., Eller, I., 1999. Thymidine dinucleotide pretreatment reduces DNA mutation frequency. Journal of Investigative Dermatology, 112, 557-569.
Labat-Robert, J., Fourtanier, A., Boyer-Lafargue, B., Robert, L., 2000. Age dependent increase of elastase type protease activity in mouse skin. Effect of UV-irradiation. Journal of Photochemistry and Photobiology. B, Biology, 57, 113–118.
Larsson, B. S., 1993. Interaction Between Chemicals and Melanin. Pigment Cell Research, 6, 127-133.
Lee, D.G., Hyun, J.W., Kang, K.A., Lee, J.O., Lee, S.H., Ha, B.J., Ha, J.M., Lee, E.Y., Lee, J.H., 2004. Ulva lactuca : A potential seaweed for tumor treatment and immune stimulation. Biotechnology and Bioprocess Engineering, 9, 236-238.
Lopez-Torres, M., Shindo, Y., Packer, L., 1994. Effect of age on antioxidants and molecular markers of oxidative damage in murine epidermis and dermis. Journal of Investigative Dermatology, 102, 476-480.
Mao, W., Zang, X., Li, Y., Zhang, H., 2006. Sulfated polysaccharides from marine green algae Ulva conglobata and their anticoagulant activity. Journal of Applied Phycology, 18, 9-14.
Marmol, V. d., Beermann, F., 1996. Tyrosinase and related proteins in mammalian Pigmentation, FEBS Letters, 381, 165-168.
Merker, K., Sitte, N., Grune, T., 2000. Hydrogen peroxidemediated protein oxidation in young and old human MRC-5 fibroblasts. Archives of Biochemistry and Biophysics, 375, 50-54.
Miller, N.J., Rice-Evans, C., Davies, M.J., Gopinathan, V., Milner, A., 1993. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical Science. 84, 407-412.
Na, Y. J., Baek, H. S., Ahn, S. M. Shin, H. J., Chang, I. S., Hwang, J. S., 2007. [4-t-Butylphenyl]-N-(4-imidazol-1-yl phenyl) sulfonamide (ISCK03) inhibits SCF/c-kit signaling in 501mel human melanoma cells and abolishes melanin production in mice and brownish guinea pigs. Biochemical Pharmacology, 74, 780-786.
Nichols, J. R., Schingoethe, D. J., Maiga, H. A., Brouk, M. J., Piepenbrink, M. S., 1998. Evaluation of Corn Distillers Grains and Ruminally Protected Lysine and Methionine for Lactating Dairy Cows. Journal of Dairy Science, 81, 482-491.
Ortiz, J., Romero, N., Robert, P., Araya, J., Lopez-Hernandez, J., Bozzo,C., Navarrete, E., Osorio, A., Rios, A., 2006. Dietary fiber, amino acid, fatty acid and tocopherol contents of the edible seaweeds Ulva lactuca and Durvillaea Antarctica. Food Chemistry, 99, 98-104.
Parvez, S., Kang, M., Chung, H. S., Cho, C., Hong, M. C., Shin, M., Bae, H., 2006. Survey and Mechanism of Skin Depigmenting and Lightening Agents. Phytotherapy Research, 20, 921-934.
Pillai, S., Oresajo. C., Hayward, J., 2005. Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation – a review. International Journal of Cosmetic Science, 27, 17-34.
Plaza, M., Santoyo, S., Jaime, L., Garcia-Blairsy Reina, G., Herrero, M., Senorans, F. J., Ibanez, E., 2009, Screening for bioactive compounds from algae. Journal of Pharmaceutical and Biomedical Analysis, [in pressed].
Qi, H., Zhang, Q.B., Zhao, T.T., Chen, R., Zhang, H., Niu, X.Z., Li, Z., 2005. Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta) in vitro. International Journal of Biological Macromolecules, 37, 195-199.
Qi, H., Zhang, Q.B., Zhao, T.T., Zhang, K., Hu, R., Li, Z., 2006. In vitro antioxidant activity of acetylated and benzoylated derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta). Bioorganic and Medicinal Chemistry Letters, 16, 2441-2445.
Rana, B. K., Hewett-Emmett, D., Li, W. H., 1996. Variation in the human melanocyte stimulating hormone receptor locus (Abstr.). Pigment Cell Research, 60, (Suppl. 5).
Reddy, L., Odhav, B., Bhoola, K. D., 2003. Natural products for cancer prevention: a global perspective. Pharmacology and Therapeutics, 99, 1-13.
Robak, J., Gryglewski, R. J., 1988. Flavonoids are scavengers of superoxide anions. Biochemical Pharmacol, 17, 837-841.
Romero, E., Bautista, J., Garc?’a-Martinez, A. M., Cremades, O., Parrado, J., 2007. Bioconversion of corn distiller’s dried grains with solubles (CDDGS) to extracellular proteases and peptones. Process Biochemistry, 42, 1492-1497.
Ryu, B. M., Qian, Z. J., Kim, M. M., Nam, K. W., Kim, S. K., 2009. Anti-photoaging activity and inhibition of matrix metalloproteinase (MMP) by marine red alga, Corallina pilulifera methanol extract. Radiation Physics and Chemistry, 78, 98–105.
Schingoethe, D. J., Brouk, M. J., Birkelo, C. P., 1999. Milk Production and Composition from Cows Fed Wet Corn Distillers Grains. Journal of Dairy Science, 82, 574-580.
Seiberg, M., Paine, C., Sharlow, E., Andrade-Gordon, P., Costanzo, M., Eisinger, M., Shapiro, S. S., 2000. Inhibition of Melanosome Transfer Results in Skin Lightening. Journal of Investigative Dermatology, 115, 162-167.
Selvin, J., Huxley, A. J., Lipton, A. P., 2003. Immunomodulatory potential of marine secondary metabolites against bacterial diseases of shrimp. Aquaculture, 230, 241-248.
Shinohara, K., Fukushima, T., Suzuki, M., Tsutsumi, M., Kobori, M., Kong, Z. L., 1993. Effect of some constituents of chicken egg yolk lipoprotein on the growth and IgM production of human-human hybridoma cells and other human-derived cells. Cytotechnology, 11, 149-154.
Tang, X. H., Vivero, M., Gudas, L. J., 2008. Overexpression of CRABPI in suprabasal keratinocytes enhances the proliferation of epidermal basal keratinocytes in mouse skin topically treated with all-trans retinoic acid. Experimental Cell Researh, 314, 38-51.
Tsai, H. H., Chen, Y. C., Lee, W. R., Hu, C. H., Hakozak, T., Yoshii, T., Shen, S. C., 2006. Inhibition of inflammatory nitric oxide production and epidermis damages by Saccharomycopsis Ferment Filtrate. Journal of Dermatological Science. 42, 249-257.
Toth, G., and Pavia, H., 2000. Lack of phlorotannin induction in the brown seaweed Ascophyllum nodosum in response to increased copper concentrations. Marine Ecology Progress Series, 192, 119-126.
Uitto, J., 1979. Biochemisty of the elastic fibres in normal connective tissues and its alterations in disease. Journal of Investigative Dermatology, 72, 1-10.
Valverde, P., Healy, E., Sikkink, S., Haldane, F., Thody, A., 1996. The Asp84Glu variant of the melanocortin 1 receptor (MC1R) is associated with melanoma. Human Molecular Genetics, 5, 1663-1666.
Wong, K.H., Cheung, P.C.K., 2000. Nutritional evaluation of some subtropical red and green seaweeds Part I-proximate composition, amino acid profiles and some physico-chemical properties. Food chemistry, 71, 475-482.
Yamamoto, T. and Nishioka, K. 2001. Animal model of sclerotic skin. Ⅳ: induction of dermal sclerosis by bleomycin is T cell independent. Journal of Investigative Dermatology, 117, 999-1001.
Yarosh, D. B., Tsimes, J., Yee, J. L., 1990. Enhancement of DNA repair of UV damage in mouse and human skin by liposomes containing a DNA repair enzyme. Journal of the Society of Cosmetic Chemists, 41, 85-92.
Yehaug, L., Plahte, E., Vage, D. I., Omholt, S. W., 2002. The Regulatory Basis of Melanogenic Switching. Journal of Theoretical Biology, 215, 449-468.
Youwei, Z., Jinlian, Z., Yonghong, P., 2008. A comparative study on the free radical scavenging activities of some fresh flowers in southern China. LWT - Food Science and Technology, 41, 1586-1591.
Yu, P.Z., Li, N., Liu, X.G., Zhou, G.F., Zhang, Q.B., Li, P.C., 2003a. Antihyperlipidemic effects of different molecular weight sulfated polysaccharides from Ulva pertusa (Chlorophyta). Pharmacological Research, 48, 543-549.
Yu, P.Z., Zhang, Q., Li, N., Xu, Z.H., Wang, Y., Li, Z., 2003b. Polysaccharides from Ulva pertusa (Chlorophyta) and preliminary studies on their antihyperlipidemia activity. Journal of Applied Phycology, 15, 21-27.
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