臺灣博碩士論文加值系統

動脈硬化發生時，於動脈新內層 (neo-intima)有新內層平滑肌細胞增生，而新內層的平滑肌細胞不同正常成熟血管平滑肌細胞，會失去收縮能力，並有了增生、移動能力和分泌大量胞外基質，此血管新內層之平滑肌型態的特異是一個重要的課題。巢蛋白是一類中間絲蛋白，專一表現在肌肉和神經的幹細胞。而本研究利用大鼠主動脈血管平滑肌細胞當做實驗材料，並以免疫螢光染色方法發現有分泌型特徵的血管平滑肌細胞會表現巢蛋白；而將細胞培養於去除血清之培養液中，血管平滑肌細胞會分化為具有收縮型平滑肌細胞的特徵，然而巢蛋白的表現明顯減弱。這個結果也暗示了巢蛋白是分泌型血管平滑肌細胞的標誌蛋白。為了要找出調節巢蛋白表現的胞外因子，利用免疫螢光染色對細胞培養的血管平滑肌細胞來做研究，結果顯示，在細胞培養中，表皮生長因子可誘導巢蛋白表現。進一步做訊息傳遞分析，顯示出表皮生長因子主要藉由活化Ras-Raf-ERK訊息路徑來誘導巢蛋白的再表現。而這是第一個研究指出在血管平滑肌細胞中，巢蛋白可藉由胞外的生長因子刺激調節。

During the progression of atherosclerosis, vascular smooth muscle cells (VSMCs) are observed in the arterial neo-intima where they proliferate. The neo-intimal smooth muscle cells are different from the mature VSMCs in that they lack of the contraction abilities, but display the characteristics of proliferation, migration, as well as the ability to secrete a large amount of extra-cellular matrix. The formation of this special phenotype of VSMCs in the neo-intima layer is a key event during the atherosclerosis.
Nestin is a intermediate filament (IF) protein specifically expressed in myogenic and neural stem cells. In this study, we showed that nestin is expressed in primary cultures of rat aortic VSMCs that represent the synthetic phenotype and its expression diminished in those cells upon differentiation to the contractile phenotype after serum deprivation.These results suggested that nestin is a marker of synthetic VSMCs. To identify the extracellular factors that regulate nestin expression, immunofluorescence microscopy was used. Results showed that epidermal growth factor (EGF) induced nestin expression in serum-deprived primary cultures of VSMCs. Furthermore, signaling analyses revealed that the EGF-induced nestin re-expression is mediated through the activation of the Ras-Raf-ERK signaling axis. This is the first report showing that nestin expression is regulated by the extracellular growth factors in VSMCs.

頁數
誌謝…………………………………………………………………………......i
英文摘要……………………………………………………………………….ii
中文摘要………………………………………………………………………iii
目次……………………………………………………………………………iv
緒論
前言......................................................................................................................1
血管平滑肌細胞的型態轉變和動脈硬化關係..................................................2
血管平滑肌型態的標誌蛋白..............................................................................6
細胞骨骼..............................................................................................................9
中間絲蛋白.........................................................................................................11
Nestin...................................................................................................................14
EGF和EGF receptor...........................................................................................18
EGF receptor之傳導路徑...................................................................................20
實驗目的.............................................................................................................23
實驗材料
實驗細胞.............................................................................................................24
細胞培養材料.....................................................................................................24
免疫螢光染色材料.............................................................................................25
抗體.....................................................................................................................25
藥品.....................................................................................................................26
實驗方法
取大鼠主動脈血管平滑肌細胞進行細胞培養..................................................27
血管平滑肌細胞培養..........................................................................................28
免疫螢光顯微鏡技術學......................................................................................28
細胞藥劑處理......................................................................................................29
偵測細胞增生處理..............................................................................................29
細胞免疫螢光染色資料統計分析......................................................................30
實驗結果
大鼠主動脈血管平滑肌細胞的確認與觀察.....................................................30
偵測血管平滑肌細胞之nestin表現..................................................................31
血管平滑肌細胞進行serum free處理之型態改變..........................................31
Nestin在血管平滑肌細胞進行serum free處理之表現變化
與血管平滑肌細胞型態轉變之關係................................................................32
EGF誘導serum free後的血管平滑肌細胞之nestin再表現.........................33
EGF 促進nestin再表現之濃度效應...............................................................34
EGF對血管平滑肌細胞之nestin表現和其型態轉變影響............................34
EGF 藉由活化EGF receptor來誘導nestin再表現.......................................36
EGF誘導nestin的表現需要經由蛋白質新生................................................37
EGF誘導nestin的再表現主要藉由Ras-Raf 路徑........................................37
EGF促進nestin的再表現主要藉由Ras-Raf-ERK 路徑..............................38
討論
血管平滑肌細胞nestin的調控.........................................................................40
血管平滑肌細胞型態轉變與其nestin表現之關聯.........................................43
Nestin對血管平滑肌細胞之可能功能探討.....................................................44
Nestin之表現和動脈硬化的關聯探討.............................................................45
未來展望............................................................................................................48
參考文獻..................................................................................................................49
附圖...........................................................................................................................61

Abouhamed, M., S. Reichenberg, H. Robenek, and G. Plenz. 2003. Tropomyosin 4 expression is enhanced in dedifferentiating smooth muscle cells in vitro and during atherogenesis. Eur J Cell Biol. 82:473-82.
Adam, P.J., C.P. Regan, M.B. Hautmann, and G.K. Owens. 2000. Positive- and negative-acting Kruppel-like transcription factors bind a transforming growth factor beta control element required for expression of the smooth muscle cell differentiation marker SM22alpha in vivo. J Biol Chem. 275:37798-806.
Aikawa, M., P.N. Sivam, M. Kuro-o, K. Kimura, K. Nakahara, S. Takewaki, M. Ueda, H. Yamaguchi, Y. Yazaki, M. Periasamy, and et al. 1993. Human smooth muscle myosin heavy chain isoforms as molecular markers for vascular development and atherosclerosis. Circ Res. 73:1000-12.
Antczak, M., J. Van Blerkom, and A. Clark. 1997. A novel mechanism of vascular endothelial growth factor, leptin and transforming growth factor-beta2 sequestration in a subpopulation of human ovarian follicle cells. Hum Reprod. 12:2226-34.
Balica, M., K. Bostrom, V. Shin, K. Tillisch, and L.L. Demer. 1997. Calcifying subpopulation of bovine aortic smooth muscle cells is responsive to 17 beta-estradiol. Circulation. 95:1954-60.
Bennett, G.S., S.A. Fellini, J.M. Croop, J.J. Otto, J. Bryan, and H. Holtzer. 1978. Differences among 100-A filamentilament subunits from different cell types. Proc Natl Acad Sci U S A. 75:4364-8.
Bernardo, J., A.M. Billingslea, M.F. Ortiz, K.F. Seetoo, J. Macauley, and E.R. Simons. 1997. Adherence-dependent calcium signaling in monocytes: induction of a CD14-high phenotype, stimulus-responsive subpopulation. J Immunol Methods. 209:165-75.
Blank, R.S., M.M. Thompson, and G.K. Owens. 1988. Cell cycle versus density dependence of smooth muscle alpha actin expression in cultured rat aortic smooth muscle cells. J Cell Biol. 107:299-306.
Bohm, C.M., M.C. Mulder, R. Zennadi, M. Notter, A. Schmitt-Graff, O.J. Finn, J. Taylor-Papadimitriou, H. Stein, H. Clausen, E.O. Riecken, and C. Hanski. 1997. Carbohydrate recognition on MUC1-expressing targets enhances cytotoxicity of a T cell subpopulation. Scand J Immunol. 46:27-34.
Braunwald, E. 1997. Shattuck lecture--cardiovascular medicine at the turn of the millennium: triumphs, concerns, and opportunities. N Engl J Med. 337:1360-9.
Breslow, J.L. 1997. Cardiovascular disease burden increases, NIH funding decreases. Nat Med. 3:600-1.
Calafat, J., H. Janssen, M. Stahle-Backdahl, A.E. Zuurbier, E.F. Knol, and A. Egesten. 1997. Human monocytes and neutrophils store transforming growth factor-alpha in a subpopulation of cytoplasmic granules. Blood. 90:1255-66.
Cantley, L.C. 2002. The phosphoinositide 3-kinase pathway. Science. 296:1655-7.
Cao, X.S., Z. Zou, and W. Yu. 1997. [In vitro expansion of human umbilical cord blood hematopiotic cells and its subpopulation]. Zhonghua Yi Xue Za Zhi. 77:422-4.
Cardenas, C.G., L.P. Del Mar, B.Y. Cooper, and R.S. Scroggs. 1997. 5HT4 receptors couple positively to tetrodotoxin-insensitive sodium channels in a subpopulation of capsaicin-sensitive rat sensory neurons. J Neurosci. 17:7181-9.
Chamley-Campbell, J., G.R. Campbell, and R. Ross. 1979. The smooth muscle cell in culture. Physiol Rev. 59:1-61.
Chamley-Campbell, J.H., G.R. Campbell, and R. Ross. 1981. Phenotype-dependent response of cultured aortic smooth muscle to serum mitogens. J Cell Biol. 89:379-83.
Chan, A.K., A. Kalmes, S. Hawkins, G. Daum, and A.W. Clowes. 2003. Blockade of the epidermal growth factor receptor decreases intimal hyperplasia in balloon-injured rat carotid artery. J Vasc Surg. 37:644-9.
Chou, M.C., S.C. Lee, Y.S. Lin, and H.Y. Lei. 1997. V beta 8+CD4-CD8- subpopulation induced by staphylococcal enterotoxin B. Immunol Lett. 55:85-91.
Chou, Y.H., S. Khuon, H. Herrmann, and R.D. Goldman. 2003. Nestin promotes the phosphorylation-dependent disassembly of vimentin intermediate filaments during mitosis. Mol Biol Cell. 14:1468-78.
Clarke, M., and M. Bennett. 2006. The emerging role of vascular smooth muscle cell apoptosis in atherosclerosis and plaque stability. Am J Nephrol. 26:531-5.
Clarke, M.C., N. Figg, J.J. Maguire, A.P. Davenport, M. Goddard, T.D. Littlewood, and M.R. Bennett. 2006. Apoptosis of vascular smooth muscle cells induces features of plaque vulnerability in atherosclerosis. Nat Med. 12:1075-80.
Corjay, M.H., M.M. Thompson, K.R. Lynch, and G.K. Owens. 1989. Differential effect of platelet-derived growth factor- versus serum-induced growth on smooth muscle alpha-actin and nonmuscle beta-actin mRNA expression in cultured rat aortic smooth muscle cells. J Biol Chem. 264:10501-6.
Cristaldi, M., M. Rovati, M. Elli, S. Gerlinzani, A. Lesma, L. Balzarotti, and A.M. Taschieri. 1997. Lymphocytic subpopulation changes after open and laparoscopic cholecystectomy: a prospective and comparative study on 38 patients. Surg Laparosc Endosc. 7:255-61.
Dahlstrand, J., L.B. Zimmerman, R.D. McKay, and U. Lendahl. 1992. Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments. J Cell Sci. 103 ( Pt 2):589-97.
Daniel, C., H. Albrecht, A. Ludke, and C. Hugo. 2008. Nestin expression in repopulating mesangial cells promotes their proliferation. Lab Invest. 88:387-97.
Doran, A.C., N. Meller, and C.A. McNamara. 2008. Role of smooth muscle cells in the initiation and early progression of atherosclerosis. Arterioscler Thromb Vasc Biol. 28:812-9.
Dreux, A.C., D.J. Lamb, H. Modjtahedi, and G.A. Ferns. 2006. The epidermal growth factor receptors and their family of ligands: their putative role in atherogenesis. Atherosclerosis. 186:38-53.
Duband, J.L., M. Gimona, M. Scatena, S. Sartore, and J.V. Small. 1993. Calponin and SM 22 as differentiation markers of smooth muscle: spatiotemporal distribution during avian embryonic development. Differentiation. 55:1-11.
Dzau, V.J., R.C. Braun-Dullaeus, and D.G. Sedding. 2002. Vascular proliferation and atherosclerosis: new perspectives and therapeutic strategies. Nat Med. 8:1249-56.
Eng, L.F., J.J. Vanderhaeghen, A. Bignami, and B. Gerstl. 1971. An acidic protein isolated from fibrous astrocytes. Brain Res. 28:351-4.
Erber, A., D. Riemer, M. Bovenschulte, and K. Weber. 1998. Molecular phylogeny of metazoan intermediate filament proteins. J Mol Evol. 47:751-62.
Fan, X.M., X.H. Jiang, Q. Gu, Y.P. Ching, H. He, H.H. Xia, M.C. Lin, A.O. Chan, M.F. Yuen, H.F. Kung, and B.C. Wong. 2006. Inhibition of Akt/PKB by a COX-2 inhibitor induces apoptosis in gastric cancer cells. Digestion. 73:75-83.
Fatigati, V., and R.A. Murphy. 1984. Actin and tropomyosin variants in smooth muscles. Dependence on tissue type. J Biol Chem. 259:14383-8.
Frid, M.G., O.Y. Printesva, A. Chiavegato, E. Faggin, M. Scatena, V.E. Koteliansky, P. Pauletto, M.A. Glukhova, and S. Sartore. 1993. Myosin heavy-chain isoform composition and distribution in developing and adult human aortic smooth muscle. J Vasc Res. 30:279-92.
Fuxe, K., B. Tinner, L. Caberlotto, B. Bunnemann, and L.F. Agnati. 1997. NPY Y1 receptor like immunoreactivity exists in a subpopulation of beta-endorphin immunoreactive nerve cells in the arcuate nucleus: a double immunolabelling analysis in the rat. Neurosci Lett. 225:49-52.
Gallo, J.C., E. Thomas, G.E. Novick, and R.J. Herrera. 1997. Effects of subpopulation structure on probability calculations of DNA profiles from forensic PCR analysis. Genetica. 101:1-12.
Grage-Griebenow, E., J. Baran, H. Loppnow, M. Los, M. Ernst, H.D. Flad, and J. Pryjma. 1997. An Fc gamma receptor I (CD64)-negative subpopulation of human peripheral blood monocytes is resistant to killing by antigen-activated CD4-positive cytotoxic T cells. Eur J Immunol. 27:2358-65.
Guerette, D., P.A. Khan, P.E. Savard, and M. Vincent. 2007. Molecular evolution of type VI intermediate filament proteins. BMC Evol Biol. 7:164.
Hara, A., M. Niwa, N. Yoshimi, and H. Mori. 1997. Apoptotic cell death in vulnerable subpopulation of cerebellar granule cells. Acta Neuropathol. 94:517-8.
Hautmann, M.B., C.S. Madsen, and G.K. Owens. 1997. A transforming growth factor beta (TGFbeta) control element drives TGFbeta-induced stimulation of smooth muscle alpha-actin gene expression in concert with two CArG elements. J Biol Chem. 272:10948-56.
Hayashi, K., M. Takahashi, K. Kimura, W. Nishida, H. Saga, and K. Sobue. 1999. Changes in the balance of phosphoinositide 3-kinase/protein kinase B (Akt) and the mitogen-activated protein kinases (ERK/p38MAPK) determine a phenotype of visceral and vascular smooth muscle cells. J Cell Biol. 145:727-40.
Hedin, U., B.A. Bottger, J. Luthman, S. Johansson, and J. Thyberg. 1989. A substrate of the cell-attachment sequence of fibronectin (Arg-Gly-Asp-Ser) is sufficient to promote transition of arterial smooth muscle cells from a contractile to a synthetic phenotype. Dev Biol. 133:489-501.
Hennebicq-Reig, S., D. Tetaert, B. Soudan, I. Kim, G. Huet, G. Briand, C. Richet, D. Demeyer, and P. Degand. 1997. O-Glycosylation and cellular differentiation in a subpopulation of mucin-secreting HT-29 cell line. Exp Cell Res. 235:100-7.
Hesse, M., T.M. Magin, and K. Weber. 2001. Genes for intermediate filament proteins and the draft sequence of the human genome: novel keratin genes and a surprisingly high number of pseudogenes related to keratin genes 8 and 18. J Cell Sci. 114:2569-75.
Holmin, S., P. Almqvist, U. Lendahl, and T. Mathiesen. 1997. Adult nestin-expressing subependymal cells differentiate to astrocytes in response to brain injury. Eur J Neurosci. 9:65-75.
Hu, W.S., E.H. Bowman, K.A. Delviks, and V.K. Pathak. 1997. Homologous recombination occurs in a distinct retroviral subpopulation and exhibits high negative interference. J Virol. 71:6028-36.
Hungerford, J.E., and C.D. Little. 1999. Developmental biology of the vascular smooth muscle cell: building a multilayered vessel wall. J Vasc Res. 36:2-27.
Ishii, S., H. Nagasawa, H. Tai, Y. Noda, K. Akiyama, H. Takeda, N. Okajima, and H. Okano. 1997. [Relationship between the activity of serum adenosine deaminase including its isozymes and lymphocyte subpopulation in patients with pulmonary tuberculosis]. Kekkaku. 72:153-9.
Isogai, S., M. Horiguchi, and B.M. Weinstein. 2001. The vascular anatomy of the developing zebrafish: an atlas of embryonic and early larval development. Dev Biol. 230:278-301.
Iushchuk, N.D., O.F. Eremina, M.V. Ostroukhova, T.A. Novikova, R.B. Tumanova, and G.K. Petina. 1997. [The characteristics of the lymphocyte subpopulation composition and its clinical significance in bacterial carriers of toxigenic Corynebacterium diphtheriae]. Zh Mikrobiol Epidemiol Immunobiol:61-4.
Jessberger, S., S. Aigner, G.D. Clemenson, Jr., N. Toni, D.C. Lie, O. Karalay, R. Overall, G. Kempermann, and F.H. Gage. 2008. Cdk5 regulates accurate maturation of newborn granule cells in the adult hippocampus. PLoS Biol. 6:e272.
Jiang, M.S., and G.W. Hart. 1997. A subpopulation of estrogen receptors are modified by O-linked N-acetylglucosamine. J Biol Chem. 272:2421-8.
Johnson, G.L., and R. Lapadat. 2002. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science. 298:1911-2.
Johnson, J.L., G.J. van Eys, G.D. Angelini, and S.J. George. 2001. Injury induces dedifferentiation of smooth muscle cells and increased matrix-degrading metalloproteinase activity in human saphenous vein. Arterioscler Thromb Vasc Biol. 21:1146-51.
Kim, S., and P.A. Coulombe. 2007. Intermediate filament scaffolds fulfill mechanical, organizational, and signaling functions in the cytoplasm. Genes Dev. 21:1581-97.
Kobayashi, S., I. Vidal, J.D. Pena, and M.R. Hernandez. 1997. Expression of neural cell adhesion molecule (NCAM) characterizes a subpopulation of type 1 astrocytes in human optic nerve head. Glia. 20:262-73.
Kroeber, S., C. Schomerus, and H.W. Korf. 1997. Calcium oscillations in a subpopulation of S-antigen-immunoreactive pinealocytes of the rainbow trout (Oncorhynchus mykiss). Brain Res. 744:68-76.
Kronin, V., G. Suss, K. Winkel, and K. Shortman. 1997. The regulation of T cell responses by a subpopulation of CD8+DEC205+ murine dendritic cells. Adv Exp Med Biol. 417:239-48.
Lankas, G.R., M.E. Cartwright, and D. Umbenhauer. 1997. P-glycoprotein deficiency in a subpopulation of CF-1 mice enhances avermectin-induced neurotoxicity. Toxicol Appl Pharmacol. 143:357-65.
Laprade, N., and J.J. Soghomonian. 1997. Glutamate decarboxylase (GAD65) gene expression is increased by dopamine receptor agonists in a subpopulation of rat striatal neurons. Brain Res Mol Brain Res. 48:333-45.
Laszik, Z.G., X.J. Zhou, G.L. Ferrell, F.G. Silva, and C.T. Esmon. 2001. Down-regulation of endothelial expression of endothelial cell protein C receptor and thrombomodulin in coronary atherosclerosis. Am J Pathol. 159:797-802.
Lee, M.S., Y.T. Kwon, M. Li, J. Peng, R.M. Friedlander, and L.H. Tsai. 2000. Neurotoxicity induces cleavage of p35 to p25 by calpain. Nature. 405:360-4.
Lefaucheur, L., R. Hoffman, C. Okamura, D. Gerrard, J.J. Leger, N. Rubinstein, and A. Kelly. 1997. Transitory expression of alpha cardiac myosin heavy chain in a subpopulation of secondary generation muscle fibers in the pig. Dev Dyn. 210:106-16.
Lendahl, U., L.B. Zimmerman, and R.D. McKay. 1990. CNS stem cells express a new class of intermediate filament protein. Cell. 60:585-95.
Li, X., V. Van Putten, F. Zarinetchi, M.E. Nicks, S. Thaler, L.E. Heasley, and R.A. Nemenoff. 1997. Suppression of smooth-muscle alpha-actin expression by platelet-derived growth factor in vascular smooth-muscle cells involves Ras and cytosolic phospholipase A2. Biochem J. 327 ( Pt 3):709-16.
Libby, P. 2002. Inflammation in atherosclerosis. Nature. 420:868-74.
Lieu, T.S., and R.D. Sontheimer. 1997. A subpopulation of WIL-2 cell calreticulin molecules is associated with RO/SS-A ribonucleoprotein particles. Lupus. 6:40-7.
Llorente, L., Y. Richaud-Patin, C. Alvarado, A. Vidaller, and J. Jakez-Ocampo. 1997. Autoantibody production in healthy elderly people is not promoted by interleukin-10 although this cytokine is expressed in them by a peculiar CD8+CD3+ large granular cell subpopulation. Scand J Immunol. 45:401-7.
Lowenstein, E.J., R.J. Daly, A.G. Batzer, W. Li, B. Margolis, R. Lammers, A. Ullrich, E.Y. Skolnik, D. Bar-Sagi, and J. Schlessinger. 1992. The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling. Cell. 70:431-42.
Lowy, D.R., and B.M. Willumsen. 1993. Function and regulation of ras. Annu Rev Biochem. 62:851-91.
Lusis, A.J. 2000. Atherosclerosis. Nature. 407:233-41.
Mahler, J., and W. Driever. 2007. Expression of the zebrafish intermediate neurofilament Nestin in the developing nervous system and in neural proliferation zones at postembryonic stages. BMC Dev Biol. 7:89.
Majesky, M.W. 2007. Developmental basis of vascular smooth muscle diversity. Arterioscler Thromb Vasc Biol. 27:1248-58.
Martin, M., H. Schoecklmann, G. Foster, L. Barley-Maloney, J. McKanna, and T.O. Daniel. 1997. Identification of a subpopulation of human renal microvascular endothelial cells with capacity to form capillary-like cord and tube structures. In Vitro Cell Dev Biol Anim. 33:261-9.
Miano, J.M. 2003. Serum response factor: toggling between disparate programs of gene expression. J Mol Cell Cardiol. 35:577-93.
Mitsumata, M., S. Gamou, N. Shimizu, and Y. Yoshida. 1994. Response of atherosclerotic intimal smooth muscle cells to epidermal growth factor in vitro. Arterioscler Thromb. 14:1364-71.
Mohanakumar, T., N. Poindexter, N. Steward, S. Shenoy, J. Lowell, and T. Howard. 1997. Tissue-specific HLA class I restricted CTL are a significant subpopulation of graft-infiltrating lymphocytes during rejection. Transplant Proc. 29:87-8.
Morshead, C.M., B.A. Reynolds, C.G. Craig, M.W. McBurney, W.A. Staines, D. Morassutti, S. Weiss, and D. van der Kooy. 1994. Neural stem cells in the adult mammalian forebrain: a relatively quiescent subpopulation of subependymal cells. Neuron. 13:1071-82.
Noh, D.Y., S.H. Shin, and S.G. Rhee. 1995. Phosphoinositide-specific phospholipase C and mitogenic signaling. Biochim Biophys Acta. 1242:99-113.
Opitz, F., K. Schenke-Layland, T.U. Cohnert, and U.A. Stock. 2007. Phenotypical plasticity of vascular smooth muscle cells-effect of in vitro and in vivo shear stress for tissue engineering of blood vessels. Tissue Eng. 13:2505-14.
Oshima, R.G. 2007. Intermediate filaments: a historical perspective. Exp Cell Res. 313:1981-94.
Owens, G.K. 1995. Regulation of differentiation of vascular smooth muscle cells. Physiol Rev. 75:487-517.
Owens, G.K., M.S. Kumar, and B.R. Wamhoff. 2004. Molecular regulation of vascular smooth muscle cell differentiation in development and disease. Physiol Rev. 84:767-801.
Pallari, H.M., and J.E. Eriksson. 2006. Intermediate filaments as signaling platforms. Sci STKE. 2006:pe53.
Palop, A., F.J. Sala, and S. Condon. 1997. Occurrence of a Highly Heat-Sensitive Spore Subpopulation of Bacillus coagulans STCC 4522 and Its Conversion to a More Heat-Stable Form. Appl Environ Microbiol. 63:2246-2251.
Pantelidis, P., A.M. Southcott, C.M. Black, and R.M. Du Bois. 1997. Up-regulation of IL-8 secretion by alveolar macrophages from patients with fibrosing alveolitis: a subpopulation analysis. Clin Exp Immunol. 108:95-104.
Peaire, A.E., A. Krantis, and W.A. Staines. 1997. Distribution of the NPY receptor subtype Y1 within human colon: evidence for NPY targeting a subpopulation of nitrergic neurons. J Auton Nerv Syst. 67:168-75.
Peerenboom, E., V. Jacobi, E.J. Cartwright, M.J. Adams, H.H. Steinbiss, and J.F. Antoniw. 1997. A large duplication in the 3''-untranslated region of a subpopulation of RNA2 of the UK-M isolate of barley mild mosaic bymovirus. Virus Res. 47:1-6.
Perez, L.J., A. Valcarcel, M.A. de Las Heras, D. Moses, and H. Baldassarre. 1997. The storage of pure ram semen at room temperature results in capacitation of a subpopulation of spermatozoa. Theriogenology. 47:549-58.
Pidkovka, N.A., O.A. Cherepanova, T. Yoshida, M.R. Alexander, R.A. Deaton, J.A. Thomas, N. Leitinger, and G.K. Owens. 2007. Oxidized phospholipids induce phenotypic switching of vascular smooth muscle cells in vivo and in vitro. Circ Res. 101:792-801.
Pinkas-Kramarski, R., I. Alroy, and Y. Yarden. 1997. ErbB receptors and EGF-like ligands: cell lineage determination and oncogenesis through combinatorial signaling. J Mammary Gland Biol Neoplasia. 2:97-107.
Pogliano, J. 2008. The bacterial cytoskeleton. Curr Opin Cell Biol. 20:19-27.
Prussin, C., and B. Foster. 1997. TCR V alpha 24 and V beta 11 coexpression defines a human NK1 T cell analog containing a unique Th0 subpopulation. J Immunol. 159:5862-70.
Quinlan, R.A., and W.W. Franke. 1982. Heteropolymer filaments of vimentin and desmin in vascular smooth muscle tissue and cultured baby hamster kidney cells demonstrated by chemical crosslinking. Proc Natl Acad Sci U S A. 79:3452-6.
Radomski, K.M., G.A. Davis, and M.H. Chandler. 1997. General versus subpopulation values in Bayesian prediction of aminoglycoside pharmacokinetics in hematology-oncology patients. Am J Health Syst Pharm. 54:541-4.
Raines, E.W. 2004. PDGF and cardiovascular disease. Cytokine Growth Factor Rev. 15:237-54.
Rasmussen, A.M., V. Horejsi, F.O. Levy, H.K. Blomhoff, E.B. Smeland, K. Beiske, T.E. Michaelsen, G. Gaudernack, and S. Funderud. 1997. CDw78--a determinant on a major histocompatibility complex class II subpopulation that can be induced to associate with the cytoskeleton. Eur J Immunol. 27:3206-13.
Reinhold, U., and H. Abken. 1997. CD4+ CD7- T cells: a separate subpopulation of memory T cells? J Clin Immunol. 17:265-71.
Reusch, P., H. Wagdy, R. Reusch, E. Wilson, and H.E. Ives. 1996. Mechanical strain increases smooth muscle and decreases nonmuscle myosin expression in rat vascular smooth muscle cells. Circ Res. 79:1046-53.
Rhodes, N.P., J.A. Hunt, and D.F. Williams. 1997. Macrophage subpopulation differentiation by stimulation with biomaterials. J Biomed Mater Res. 37:481-8.
Robinson, M.J., and M.H. Cobb. 1997. Mitogen-activated protein kinase pathways. Curr Opin Cell Biol. 9:180-6.
Ross, J.L., M.Y. Ali, and D.M. Warshaw. 2008. Cargo transport: molecular motors navigate a complex cytoskeleton. Curr Opin Cell Biol. 20:41-7.
Ross, R. 1999. Atherosclerosis--an inflammatory disease. N Engl J Med. 340:115-26.
Ross, R., and J.A. Glomset. 1973. Atherosclerosis and the arterial smooth muscle cell: Proliferation of smooth muscle is a key event in the genesis of the lesions of atherosclerosis. Science. 180:1332-9.
Rovner, A.S., R.A. Murphy, and G.K. Owens. 1986. Expression of smooth muscle and nonmuscle myosin heavy chains in cultured vascular smooth muscle cells. J Biol Chem. 261:14740-5.
Rubinstein, A. 1995. National Cholesterol Education Program, second report of the Expert Panel on detection, evaluation, and treatment of high blood cholesterol in adults. Circulation. 91:908-9.
Sahlgren, C.M., H.M. Pallari, T. He, Y.H. Chou, R.D. Goldman, and J.E. Eriksson. 2006. A nestin scaffold links Cdk5/p35 signaling to oxidant-induced cell death. EMBO J. 25:4808-19.
Schwartz, S.M., R.L. Heimark, and M.W. Majesky. 1990. Developmental mechanisms underlying pathology of arteries. Physiol Rev. 70:1177-209.
Sejersen, T., and U. Lendahl. 1993. Transient expression of the intermediate filament nestin during skeletal muscle development. J Cell Sci. 106 ( Pt 4):1291-300.
Seydoux, G., and M.A. Dunn. 1997. Transcriptionally repressed germ cells lack a subpopulation of phosphorylated RNA polymerase II in early embryos of Caenorhabditis elegans and Drosophila melanogaster. Development. 124:2191-201.
Soghomonian, J.J., and N. Laprade. 1997. Glutamate decarboxylase (GAD67 and GAD65) gene expression is increased in a subpopulation of neurons in the putamen of Parkinsonian monkeys. Synapse. 27:122-32.
Steinert, P.M., Y.H. Chou, V. Prahlad, D.A. Parry, L.N. Marekov, K.C. Wu, S.I. Jang, and R.D. Goldman. 1999. A high molecular weight intermediate filament-associated protein in BHK-21 cells is nestin, a type VI intermediate filament protein. Limited co-assembly in vitro to form heteropolymers with type III vimentin and type IV alpha-internexin. J Biol Chem. 274:9881-90.
Su, B., S. Mitra, H. Gregg, S. Flavahan, M.A. Chotani, K.R. Clark, P.J. Goldschmidt-Clermont, and N.A. Flavahan. 2001. Redox regulation of vascular smooth muscle cell differentiation. Circ Res. 89:39-46.
Szepvolgyi, J., B.E. Kravjak, L. Biro, K. Nagy, G. Biro, and M. Antal. 1997. [Cardiovascular disease-related serum lipid parameters in a Hungarian subpopulation]. Orv Hetil. 138:2005-8.
Thyberg, J., and A. Hultgardh-Nilsson. 1994. Fibronectin and the basement membrane components laminin and collagen type IV influence the phenotypic properties of subcultured rat aortic smooth muscle cells differently. Cell Tissue Res. 276:263-71.
Tobiasz-Adamczyk, B. 1997. Health beliefs and health behaviours in subpopulation of stomach cancer families and in the control group. Cancer Lett. 114:301-4.
Torkelson, J., R.S. Harris, M.J. Lombardo, J. Nagendran, C. Thulin, and S.M. Rosenberg. 1997. Genome-wide hypermutation in a subpopulation of stationary-phase cells underlies recombination-dependent adaptive mutation. EMBO J. 16:3303-11.
Umbenhauer, D.R., G.R. Lankas, T.R. Pippert, L.D. Wise, M.E. Cartwright, S.J. Hall, and C.M. Beare. 1997. Identification of a P-glycoprotein-deficient subpopulation in the CF-1 mouse strain using a restriction fragment length polymorphism. Toxicol Appl Pharmacol. 146:88-94.
Vaittinen, S., R. Lukka, C. Sahlgren, T. Hurme, J. Rantanen, U. Lendahl, J.E. Eriksson, and H. Kalimo. 2001. The expression of intermediate filament protein nestin as related to vimentin and desmin in regenerating skeletal muscle. J Neuropathol Exp Neurol. 60:588-97.
Van Bockstaele, E.J., and J. Chan. 1997. Electron microscopic evidence for coexistence of leucine5-enkephalin and gamma-aminobutyric acid in a subpopulation of axon terminals in the rat locus coeruleus region. Brain Res. 746:171-82.
Vandekerckhove, J., and K. Weber. 1979. The complete amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle, and rabbit slow skeletal muscle. A protein-chemical analysis of muscle actin differentiation. Differentiation. 14:123-33.
Vankelecom, H., P. Matthys, and C. Denef. 1997. Inducible nitric oxide synthase in the anterior pituitary gland: induction by interferon-gamma in a subpopulation of folliculostellate cells and in an unidentifiable population of non-hormone-secreting cells. J Histochem Cytochem. 45:847-57.
Viswanathan, M., K. Tsutsumi, F.M. Correa, and J.M. Saavedra. 1991. Changes in expression of angiotensin receptor subtypes in the rat aorta during development. Biochem Biophys Res Commun. 179:1361-7.
Weber, K., U. Plessmann, and W. Ulrich. 1989. Cytoplasmic intermediate filament proteins of invertebrates are closer to nuclear lamins than are vertebrate intermediate filament proteins; sequence characterization of two muscle proteins of a nematode. EMBO J. 8:3221-7.
Wittmann, T., A. Hyman, and A. Desai. 2001. The spindle: a dynamic assembly of microtubules and motors. Nat Cell Biol. 3:E28-34.
Wouters, B.G., and L.D. Skarsgard. 1997. Low-dose radiation sensitivity and induced radioresistance to cell killing in HT-29 cells is distinct from the "adaptive response" and cannot be explained by a subpopulation of sensitive cells. Radiat Res. 148:435-42.
Yamamoto, M., and K. Yamamoto. 1994. Growth regulation in primary culture of rabbit arterial smooth muscle cells by platelet-derived growth factor, insulin-like growth factor-I, and epidermal growth factor. Exp Cell Res. 212:62-8.
Yamamoto, Y., J. Livet, R.A. Pollock, A. Garces, V. Arce, O. deLapeyriere, and C.E. Henderson. 1997. Hepatocyte growth factor (HGF/SF) is a muscle-derived survival factor for a subpopulation of embryonic motoneurons. Development. 124:2903-13.
Yang, H.Y., N. Lieska, A.E. Goldman, and R.D. Goldman. 1992. Colchicine-sensitive and colchicine-insensitive intermediate filament systems distinguished by a new intermediate filament-associated protein, IFAP-70/280 kD. Cell Motil Cytoskeleton. 22:185-99.
Yang, H.Y., N. Lieska, V. Kriho, C.M. Wu, and G.D. Pappas. 1997. A subpopulation of reactive astrocytes at the immediate site of cerebral cortical injury. Exp Neurol. 146:199-205.
Zelazowska, E.B., A. Singh, R.B. Raybourne, E.M. Sternberg, P.W. Gold, and P.A. Deuster. 1997. Lymphocyte subpopulation expression in women: effect of exercise and circadian rhythm. Med Sci Sports Exerc. 29:467-73.
Zhou, W., B.O. Ibe, and J.U. Raj. 2007. Platelet-activating factor induces ovine fetal pulmonary venous smooth muscle cell proliferation: role of epidermal growth factor receptor transactivation. Am J Physiol Heart Circ Physiol. 292:H2773-81.