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研究生:張凱婷
研究生(外文):Kai-Ting Chang
論文名稱:非農藥資材對台灣金線蓮莖基腐病之防治效果
論文名稱(外文):Non-pesticide Control of Anoectochilus formosanus Stem Rot
指導教授:孫岩章孫岩章引用關係
指導教授(外文):En-Jang Sun
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:植物病理與微生物學研究所
學門:農業科學學門
學類:植物保護學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:58
中文關鍵詞:金線蓮莖基腐病非農藥資材碳酸氫鉀碳酸氫鈉精油鐮胞菌
外文關鍵詞:Anoectochilus formosanusstem rotnon-pesticidebicarbonatesFusarium spp.essential oil
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金線蓮莖基腐病(stem rot)普遍發生於金線蓮(Anoectochilus formosanus)的栽培場,不論株齡大小皆會受其危害,為金線蓮生產栽培上主要限制因子。因金線蓮是重要中藥材,可全株入藥,且在植物保護手冊無正式推薦藥劑,故本研究擬探討利用非農藥資材對此一莖基腐病防治之可行性。在培養皿上利用十種精油及竹醋液測定對莖基腐病原菌抑制生長之結果,發現濃度1000ppm之肖楠精油具有100%的生長抑制效果。利用七種非農藥資材防治配方,分別為(1) 2000ppm KHCO3(添加1% Tween 20)、(2) 2000ppm NaHCO3(添加1% Tween 20)、(3) 2000ppm 肖楠精油(添加1% Tween 20)、(4) 1000ppm KHCO3(添加1% Tween 20)、(5) 1000ppm NaHCO3(添加1% Tween 20)、(6) 1000ppm肖楠精油 (添加1% Tween 20)、(7)1000ppm (KHCO3 + Tween 20),進行盆栽接種病原之防治試驗,發現1000ppmNaHCO3 + 1%Tween 20、2000ppm NaHCO3 + 1%Tween 20及1000ppm (KHCO3 + Tween 20)與對照組相比皆具有防治之效果,其植株存活率達33%,對照組之存活率僅27%,為對照組之1.2倍,此防治效果優於化學藥劑腐絕。施用500ppm 41.8% 腐絕(Thiabendazole)做為負對照組,其存活率只有20%。在盆栽未接種病原菌之防治試驗中則以噴施2000ppm KHCO3+1% Tween 20之防治效果最佳,防治率為46%。另在種植前做碳酸氫鉀水溶液浸泡根部處理及噴施處理皆可減低發病率約20%。綜合上述試驗結果可知非農藥資材對金線蓮莖基腐病仍具有某些程度之防治效果,未來在金線蓮莖基腐病防治上極具有推廣之潛力。



The stern rot of Anoectochilus formosanus caused by what ? Occurs frequently in the greenhouse in Taiwan. This disease has become the limiting factor in the cultivation of the Anoectochilus formosanus. The Anoectochilus formosanus is an important Chinese medicinal plant and the which plant can be made into the medicine. However, there is no recommended pesticide prescription in the plant protection manual. This research is to test different non-pesticide materials for their effectiveness in controlling this disease. Ten essential oils and bamboo vinegar were evaluated for their growth inhibition againest of the pathogens. Results showed that the essential oil from Taiwan incense cedar can 100% inhibit the mycelium growth of this pathogen. Seven non-pesticide materials including (1) 2000 ppm KHCO3 (plus 1% Tween 20), (2) 2000 ppm NaHCO3 (plus 1% Tween 20), (3) 2000 ppm Taiwan incense ceda (plus 1% Tween 20), (4) 1000 ppm KHCO3(plus 1% Tween 20) , (5) 1000 ppm NaHCO3 ( plus 1% Tween 20) , (6) 1000 ppm Taiwan incense ceda (plus 1% Tween 20) , and (7)1000 ppm (KHCO3 + Tween 20) wew tested in the inoculated potted plants, the results showed that the survival rates of the herb at 1000 ppm NaHCO3 (plus 1% Tween 20)、2000 ppm NaHCO3 (plus 1% Tween 20) or 1000 ppm (KHCO3 + Tween 20) were over 33%. The treatment with 500 ppm 41.8% Thiabendazole showed only 20% survival rate. In non-inoculated plant control trials best control treatment were spray of 2000 ppm KHCO3+1% Tween 20 with the control rate of 46%. The treatment of herb seedling with KHCO3, either by dipping in the KHCO3solution or spraying with KHCO3 , showed 20% reduction of disease incidence. These results suggest that non-pesticide materials are to some extent capable of controlling this serious disease of the stem rot. This approach can be recommended to farmers for the stem rot control of the Anoectochilus formosanus.

誌謝…………………………………………………………………………..i
中文摘要.........................................................................................................ii
英文摘要........................................................................................................iii
第一章、前言..................................................................................................1
第二章、前人研究..........................................................................................6
一、台灣金線蓮病害簡介..........................................................................6
(一) 莖基腐病.........................................................................................6
(二) 基腐病.............................................................................................7
二、利用非農藥物質防治鐮胞菌引起之病害..........................................9
(一) 植物萃取液.....................................................................................9
(二) 油類.................................................................................................9
(三) 無機鹽類.......................................................................................10
第三章、材料與方法....................................................................................16
一、台灣金線蓮莖基腐田間病害調查與病原菌之分離......................16
二、金線蓮莖基腐病之病原性測定方法................................................17
(一) 供試植物及供試菌株之來源.......................................................17
(二) 金線蓮莖基腐病分離株孢子懸浮液噴施地上部之病原性測定...................................................................................................18
(三) 金線蓮莖基腐病分離株孢子懸浮液浸根處理之病原性測定.................................................................................................18
(四) 金線蓮莖基腐病分離株孢子懸浮液拌入土壤之病原性測定...................................................................................................18
三、由迷迭香分離之Pythium myriotylum對台灣金線蓮之病原性
測定.......................................................................................18
(一) 供試菌株之來源...........................................................................18
(二) 由迷迭香分離之Pythium myriotylum游走子懸浮液拌入土壤之病原性測定………………………...............................................19
四、十種精油及竹醋液平板培養基對金線蓮莖基腐病菌之生長抑制試驗.........................................................................................................20
(一) 精油平板培養基及竹醋液平板培養基之製備...........................20
(二) 精油平板培養基及竹醋液對台灣金線連莖基腐病菌之生長
抑制試驗………...........................................................................20
五、浸泡碳酸氫鉀及噴施碳酸氫鉀對金線蓮莖基腐病菌之防治試驗.......................................................................................................20
六、重碳酸鹽類及肖楠精油對台灣金線蓮莖基腐病之盆栽防治試驗.......................................................................................................21
(一) 非農藥防治資材及噴施器具之取得..........................................21
(二) 非農藥防治資材藥劑之調配......................................................21
(三) 藥劑之噴施..................................................................................22
(四) 藥害評估......................................................................................22
第四章、結果...............................................................................................23
一、台灣金線蓮莖基腐田間病害調查與病原菌之分離.......................23
二、金線蓮莖基腐病之病原性測定.......................................................24
(一) 金線蓮莖基腐病分離株孢子懸浮液噴施地上部之病原性測定...................................................................................................24
(二) 金線蓮莖基腐病分離株孢子懸浮液浸根處理之病原性測定...................................................................................................24
(三) 金線蓮莖基腐病分離株孢子懸浮液拌土之病原性測定…......24
三、由迷迭香分離之Pythium myriotylum對台灣金線蓮之病原性測定.......................................................................................................25
(一) 供試菌株游走子懸浮液之製備結果……………......................25
(二) 由迷迭香分離之Pythium myriotylum游走子懸浮液拌土之
病原性測定……….......................................................................25
四、十種精油及竹醋液平板培養基對金線蓮莖基腐病菌之生長抑制試驗.........................................................................................................26
五、浸泡碳酸氫鉀及噴施碳酸氫鉀對金線蓮莖基腐病菌之防治試驗.......................................................................................................27
六、重碳酸鹽類及肖楠精油對台灣金線蓮莖基腐病之盆栽防治試驗………………………………………………………...................27
(一) 重碳酸鹽類對台灣金線蓮莖基腐病之防治效果......................27
(二) 肖楠精油對台灣金線蓮莖基腐病之防治效果..........................28
第五章、 討論.............................................................................................43
第六章、參考文獻.......................................................................................46


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