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研究生:吳宛玲
研究生(外文):Wan-Ling Wu
論文名稱:椰香天竺葵葉片揮發性成分及其遺傳分析
論文名稱(外文):Leaf volatile components and inheritance analyses inPelargonium grossularioides (L.) L’Hér. ex Ait.
指導教授:張祖亮張祖亮引用關係
指導教授(外文):Tsu-Liang Chang
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:園藝學研究所
學門:農業科學學門
學類:園藝學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:86
中文關鍵詞:椰香天竺葵頂空固相微萃取法廣義遺傳力
外文關鍵詞:Pelargonium grossularioidesSPMEbroad heritability
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本研究利用頂空固相微萃取法(Headspace solid phase microextraction, HS-SPME)及氣相層析質譜儀(Gas chromatography/Mass spectrum,GC/MS),分析椰香天竺葵(Pelargonium grossularioides)葉片揮發性香氣成分且確立其分析方法。初步探討葉片主成分cis-3-hexenol、myrcene、3-hexen-1-ol[acetate]、limonene、L-fenchone、cyclohexanone與δ-cadinene之遺傳行為。首先,確立分析方法及其流程:取40片直徑4 mm之葉圓片置25 mL之頂空瓶;加入內標準品後,以75℃加熱20分鐘,再以SPME吸附20分鐘;SPME脫附時間為30秒。樣品採樣後不宜儲藏應立即分析,取樣當日採樣時間對七種主成分含量影響小。
採收種子成熟度指標為J之種子且採收後立即播種,其發芽率佳可近100%。種子儲藏溫度以18℃較5℃為佳,18℃儲藏者之發芽率會隨儲藏日數增加而提高,儲藏28天後播種發芽率為80.5%。5℃儲藏16週之最高發芽率僅44.5%,儲藏日數增加會降低發芽率且延遲發芽。
以GC/MS分析親本、自交一代、全互交後裔及扦插苗葉片揮發性香氣,得校正後含量對數指標(Calibrated Abundance Log Index,CALI)。七種葉片於主成分自交一代和全互交後裔族群之分布,其趨勢近常態分布。自交一代和全互交後裔各主成分間具相關性,尤其以 myrcene、limonene、L-fenchone、cyclohexanone及δ-cadinene之相關相較高。利用主成分分析法 (Principal component analysis)之因子分析,計算各獨立的主成分。自交一代和全互交後裔族群之資料可被3個無法觀察的因子解釋,累積的解釋量分別達原變方的88.9%及95.4%。將3個因子經過Varimax轉軸後,顯示第1個因子(F1)與葉片主成分myrcene、limonene、L-fenchone、cyclohexanone及δ-cadinene有關,第2個因子(F2)與cis-3-hexenol有關,而第3個因子(F3)與3-hexen-1-ol[acetate]有關。以變異範圍最大的第1主成分值為基準進行篩選,選拔最大極端值H及最小極端值L為雜交親本,以兩單株進行2 X 2全互交試驗。H × H和L × L兩族群與3個因子之關係,兩族群有明顯分離,表示選拔有效。自交一代和全互交後裔七種葉片主成分與三因子間之關係,兩族群之趨勢相同,主要分為兩大群,一為cis-3-hexenol和3-hexen-1-ol[acetate],另一為myrcene、limonene、L-fenchone、cyclohexanone及δ-cadinene。
各成分之廣義遺傳率,以單株繁殖營系之方法較具效力。當分析單株時(r = 1),七種葉片主成分之廣義遺傳率最小值為11.7%(L-fenchone),最大值為43.2%(δ-cadinene)。若分析以各單株繁殖之營養系株數為4株時(r = 4),則各成分之廣義遺傳力可提高約2~3倍。
Leaf volatile components analysis by headspace solid phase microextraction (SPME) and gas chromatography-mass spectrum (GC-MS) of Pelargonium grossularioides. Furthermore, structure a method for the analysis. To discuss with heritability of cis-3-hexenol, myrcene, 3-hexen-1-ol[acetate], limonene, L-fenchone, cyclohexanone, and δ-cadinene. First, samling 40 discs of 4 mm diametral in 25 mL vial; heating 20 minute at 75℃ after add internal standards and then trap 20 minute by SPME; desorb 20 minute. The samples should analyze immediately after sampling.
The germination percentage was 100% nearly if seeding immediately after pick with seed meaturity index J. Seed storage temperature at 18℃ was better then at 5℃. The germination percentage at 18℃ was raised with inceease storage time. The germination rate 80.5% which was process 28 days to storage at 18℃. The highest germination percentage was only 44.5% process 16 weeknesses to storage at 5℃. The germination percentage decease and delay with increase storage times.
CALI were analyzed of leaf major volatile components by GC/MS of parents, selfing progeny, reciprocal crosses hybrids, and clone in P. grossularioides. Seven leaf major components trend to normal distribution of selfing progeny and reciprocal crosses hybrids of P. grossularioides. There were related to seven major leaf components, especially in myrcene, limonene, L-fenchone, cyclohexanone, and δ-cadinene. Principal component individual were counted by factor analysis of principal component analysis. The date could be explained to three factors of selfing progeny and reciprocal crosses hybrids. The cumulative were 88.9%及95.4% individual of eigenvalue. Three factors to transform by Varimax, F1 correlates with myrcene, limonene, L-fenchone, cyclohexanone, and δ-cadinene; F2 correlates with cis-3-hexenol; F3 correlates with 3-hexen-1-ol[acetate]. Selection extreme individuals, H and L, base on variable F1. Reciprocal crosses testing with H and L. Two populations, H × H和L × L, correlates with three factors, and they segregate clear. That show selection is effective.
The broad heritability is effective to propagate clone of seven major components. The minimum of broad heritability is 11.7% (L-fenchone) and the maximum is 43.2%(δ-cadinene)when analyze a single plant (r=1). The broad heritability could increase triple when analyze 4 clone of the single plant (r=4).
誌 謝 I
摘 要 II
Abstract IV
目 錄 VI
圖目錄 IX
表目錄 XIV
化學名稱中英文對照表(List) XVII
第一章、 前言(Introduction) 1
第二章、 前人研究(Literature review) 3
壹、 天竺葵屬植物之分類地位 3
貳、 天竺葵精油及其香氣成分 4
參、 影響天竺葵精油產量及其香氣成分之因子 5
肆、 椰香天竺葵的植物特性 8
伍、 天竺葵屬植物成分的藥理作用 9
陸、 香氣成分之遺傳 10
柒、 天竺葵屬植物種子之休眠性 12
捌、 精油之萃取及其香氣成分分析方法 14
第三章、 材料與方法(Materials and Methods) 18
壹、 試驗材料 18
一、 親本植株(共5株,代號P1∼P5) 18
二、 自交一代植株 18
三、 單株選拔 19
四、 全互交後裔植株 20
五、 自交一代之無性繁殖株 21
貳、 試驗項目 21
一、 儲藏日數與儲藏溫度對椰香天竺葵種子發芽之影響 21
二、 椰香天竺葵葉片揮發性香氣成分分析方法之確立 22
三、 椰香天竺葵葉片揮發性香氣成分分析方法 24
四、 椰香天竺葵葉片揮發性香氣成分之遺傳分析 25
第四章、 結果與討論(Results and discussion) 28
壹、 儲藏日數與儲藏溫度對椰香天竺葵種子發芽之影響 28
貳、 雜交授粉 29
參、 椰香天竺葵葉片揮發性香氣成分分析方法 30
一、 取樣葉序的確立 30
二、 樣品製備條件的確立 31
三、 香氣成分一日內之變化 33
四、 內標準品的選用 33
肆、 椰香天竺葵葉片揮發性香氣成分 34
伍、 椰香天竺葵自交一代植株 35
一、 葉片揮發性香氣成分分析 35
二、 七種主成分之相關性 37
三、 主成分法之因子分析 37
陸、 椰香天竺葵全互交後裔植株 38
一、 葉片揮發性香氣成分分析 38
二、 七種主成分之相關性 39
三、 主成分法之因子分析 39
柒、 椰香天竺葵葉片七種主成分之廣義遺傳力 40
第五章、 結論(Conclusion) 80
第六章、 參考文獻(Reference) 83
附錄 1. 2007年11月至2008年9月,溫室內之每日平均溫度、每日最高溫及每日最低溫 i
1.王升陽. 2006. 木材香味成分研究之新進展. 林業研究專訊 13:5-9.
2.中華民國行政院環境保護署. 2002. 半揮發性有機物檢測方法-毛細管柱氣相層析質譜儀法(NIEA M731.00C).
3.李坤、郭修武、謝洪剛、郭印山、李軼輝、李成祥. 2005. 葡萄自交與雜交後裔香氣成分的遺傳研究. 園藝學報 32:218-221.
4.李記明、賀普超. 2002. 葡萄種間雜交香味成分的遺傳研究. 園藝學報 29:9-12.
5.吳香霖. 2008. 以葉片揮發性化合物組成及SSR分子標誌鑑別台灣地區酪梨栽培種之親緣關係. 國立臺灣大學園藝學研究所碩士論文.
6.孫佛、瞿佛菁. 2002. 香葉天竺葵的藥理研究進展. 中藥材 25:600-602.
7.張定霖、洪進雄、吳招祥. 2003. 香藥草植物圖鑑. 行政院農業委員會種苗改良繁殖場.
8.廖俊銘. 2008. 香葉天竺葵Pelargonium graveolens及P. capitatum cv. Attar of Rose之葉片香氣成分分析:葉齡及白化處理的影響. 國立臺灣大學園藝學研究所碩士論文.
9.管靜如. 1993. 季節與高溫對天竺葵生長、開花品質及環境美化之影響. 國立臺灣大學園藝學研究所碩士論文.
10.Araya, H. T., P. Soundy, and M. Steyn. 2006. Response of herbage yield, essential oil yield and composition of South African rose-scented geraium (Pelargonium sp.) to conventional and organic nitrogen. J. Essent. Oil Res. 18:111-115.
11.Baskin, J. M. and C. C. Baskin. 1974. Some eco-physiological aspects of seed dormancy in Geraniaceae carolianm L. from Cental Tennessee. Oecologia 16:209-219.
12.Bayramoglu, B., S. Sahin, and G. Sumnu. 2008. Solvent-free microwave extraction of essential oil from oregano. J. Food Eng. 88:535-540.
13.Beltran, G., M. P. Aguilera, and M. H. Gordon. 2005. Solid phase microextraction of volatile oxidation compounds in oil-in-water emulsions. Food Chem. 92:401-406.
14.Chisowa, E. H. and W. K. Chishimba. 1999. A comparison of the oils of rose-scented geranium (Pelargonium sp.) propagated by tissue culture and cuttings. J. Essent. Oil Res. 11:461.
15.Choi, H. S. 2003. Character impact odorants of citrus hallabong [(C. unshiu Marcov x C. sinensis Osbeck) x C. reticulata Blanco] cold-pressed peel oil. J. Agric. Food Chem. 51:2687-2692.
16.Doimo, L., D. C. Mackay, G. B. Rintoul, B. R. D. Aray, and R. J. Fletcher. 1999. Citronellol: geraniol ratios and temperature in geranium (Pelargonium hybrid). J. Hort. Sci. Biotech. 74:528-530.
17.Farag, M. A. 2008. Headspace Analysis of Volatile Compounds in Leaves from the Juglandaceae (Walnut) Family. J. Essent. Oil Res. 20:323-327.
18.Gómeza, O. C., J. M. López, and M. G. López. 2004. Solid-phase microextraction–gas chromatographic–mass spectrometric analysis of garlic oil obtained by hydrodistillation. J. Chromato. A 1036:91-93.
19.Hanover, J. W. 1966a. Genetics of terpenes. I. Gene control of Monoterpene levels in Pinus monticola Doufl. Heredity 21:73-84.
20.Hanover, J. W. 1966b. Inheritance of 3-carene concentration in Pinus monticola. Forest Sci. 12:447-450.
21.Jirovetz, L., G. Buchbauer, A. Stoyanova, A. Balinova, Z. Guangjiun, and M. Xihan. 2005. Solid phase microextraction/gas chromatographic and olfactory analysis of the scent and fixative properties of the essential oil of Rosa damascena L. from China. Flavour Fragr. J. 20:7-12.
22.Juliani, H. R., A. Koroch, and J. E. Simon. 2006. Quality of geranium oils (Pelargonium species):case studies in Southern and Eastern Africa. J. Essent. Oil. Res. 18:116-121.
23.Ledauphin, J., H. Guichard, J. F. Saint-Clair, B. E. Picoche, and D. Barillier. 2003. Chemical and Sensorial Aroma Characterization of Freshly Distilled Calvados. 2. Identification of Volatile Compounds and Key Odorants. J. Agric. Food Chem. 51:433-442.
24.Lis-Balchin, M. 1993. The essential oils of Pelargonium grosssularioides and Erodium cicutarium (Geraniaceae). J. Essent. Oil Res. 5:317-318.
25.Lis-Balchin, M. 1996. Geranium oil. The Interational Journal of Aromatherapy 7:18-20.
26.Lis-Balchin, M. 2002a. Essential oils from different Pelargonium species and cultivars: their chemical composition (using GC, GC/MS) and appearance of trichomes (under EM). Geranium and Pelargonium: 147-165.
27.Lis-Balchin, M. 2002b. Geranium oil and its use in aromatherapy. Geranium and Pelargonium: 234-246.
28.Lis-Balchin, M., S. L. Hart, S. G. Deans, and E. Eaglesham. 1995. Potenial agrochemical and medical usage of essential oils of Pelargonium Species. J. Herbs Spices Med. Plants 3:11-22.
29.Lis-Balchin, M. T. and S. L. Hart. 1994. A pharmacological appraisal of the folk medicinal usage of Pelargonium grossularioides and Erodium cicutarium. J. Herbs Spices Med. Plants 2:41-48.
30.Lucchesi, M. E., F. Chemat, and J. Smadja. 2004. Solvent-free microwave extraction of essential oil from aromatic herbs: comparison with conventional hydro-distillation. J. Chromato. A 1043:323-327.
31.Lucchesi, M. E., J. Smadja, S. Bradshaw, W. Louw, and F. Chemat. 2007. Solvent free microwave extraction of Elletaria cardamomum L.: A multivariate study of a new technique for the extraction of essentail oil. J. Food Eng. 79:1079-1086.
32.Meisert, A. 2002. Physical dormancy in Geraniacese seeds. Seed Science Research 12:121-128.
33.Miller, D. M. 2002. The taxonomy of Pelargonium species and cultivars, their origins and growth in the wild. Geranium and Pelargonium:49-79.
34.Motsa, N. M., P. Soundy, and J. M. Steyn. 2006. Plant shoot age and temperanture effects on essential oil yield and oil composition of rose-scented geranium (Pelargonium sp.) grown in South Africa. J. Essent. Oil Res. 18:106-110.
35.Paraschivescu, M. C., E. G. Alley, W. T. French, R. Hernandez, and K. Armbrust. 2008. Determination of methanol in biodiesel by headspace solid phase microextraction. Biores. Tech. 99:5901-5905.
36.Peterson, A., S. Machmudah, B. C. Roy, M. Goto, M. Sasaki, and T. Hirose. 2005. Extraction of essential oil from geranium (Pelargonium graveolens) with supercritical carbon dioxide. J. Chem. Technol. Biotechnol. 81:167-172.
37.Peterson, A., S. Machmudah, B. C. Roy, M. Goto, M. Sasaki, and T. Hirose. 2006. Extraction of essential oil from geranium (Pelargonium graveolens) with supercritical carbon dioxide. J. Chem. Technol. Biotechnol. 81:167-172.
38.Rajeswara Rao, B., A. Bhattacharya, and P. Kaul. 2001. Yield and chemical composition of rose-scented geranium (Pelargonium species) oil at different times of harvesting. J. Essent. Oil Res. 13:456-459.
39.Rajeswara Rao, B., A. Bhattacharya, and P. N. Kaul. 1993. Changes in profiles of essential oils of rose-scented geranium (Pelargonium sp.) durin leaf ontogeny. J. Essent. Oil Res. 5:301-304.
40.Rajeswara Rao, B. and A. K. Bhattcharya. 1997. Yield and chemical composition of essential oil of rose-scented geranium (Pelargonium species) grown in the presence and absence of weeds. Flavour Fragr. J. 12:201-204.
41.Rajeswara Rao, B. R. and A. K. Bhattcharya. 1999. The impact of wilt disease on oil yield and quality of two cultivars of rose-scented geranium (Pelargonium species). J. Essent. Oil Res. 11:769.
42.Rajeswara Rao, B. R., P. N. Kaul, G. R. Mallavarapu, and S. Ramesh. 2000. First observation of little leaf disease and its impact on the yield and composition of the essential oil of rose-scented geranium (Pelargonium sp.). Flavour Fragr. J. 15:137-140.
43.Rajeswara Rao, B. R., P. N. Kaul, G. R. Mallavarapus, and S. Rameshs. 1996. Effect of seasonal climatic changes on biomass yield and terpenoid composition of rose-scented geranium (Pelargonium species). Bioche. Sys. Ecol. 24:627-635.
44.Rana, V., J. P. Juyal, and M. A. Blazquez. 2002. Chemical constituents of essential oil of Pelargonium graveolens leaves. The Interational Journal of Aromatherapy 12:216-218.
45.Rajeswara Rao, B. R. and A. K. Bhattcharya. 1999. The impact of wilt disease on oil yield and quality of two cultivars of rose-scented geranium (Pelargonium species). J. Essent. Oil Res. 11:769.
46.Saxena, G., S. Banerjee, L. Rahman, G. R. Mallavarapu, S. Sharma, and S. Kumar. 2000. An dfficient in vitro procedure for micropropagation and generation of somaclones of rose scented Pelargonium. Plant Science 155:133-140.
47.Strauss, S. H. and W. B. critchfield. 1982. Inheritance of β-pinene in xylem oleoresin of Knobcone x Momterey pine hybrids. Forest Sci. 28:687-696.
48.Van Assche, J. A. and F. E. A. Vandelook. 2006. Germination ecology of eleven species of Geraniaceae and Malvaceae with special reference to the effects of drying seeds. Seed Science Research 16:283-290.
49.Wajs, A., A. Pranovich, M. Reunanen, S. Willför, and B. Holmbom. 2007. Headspace-SPME analysis of the sapwood and heartwood of Picea Abies, Pinus Sylvestris and Larix Decidua. J. Essent. Oil Res. 19:125-133.
50.Washitani, I. 1985. Germination-rate dependency on temperature of Geranium carolinianum. J. Exp. Bot. 36:330-337.
51.Yamini, Y., M. Khajeh, E. Ghasemi, M. Mirza, and K. Javidnia. 2008. Comparison of essential oil compositions of Salvia mirzayanii obtained by supercritical carbon dioxide extraction and hydrodisrillarion methods. Food Chem. 108:341-346.
52.Yazdani, R., D. Rudin, T. Alden, B. Harbom, and K. Ljung. 1982. Inheritance pattern of five monoterpenes in Scots pine (Pinus sylvestris L.). Hereditas 97:261-272.
53.Gardenweb. http://faq.gardenweb.com/faq/lists/geranium/2002075804001127.html
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