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研究生:魏君翰
研究生(外文):Jyun-Han Wei
論文名稱:玻璃製造廠勞工職業暴露對DNA氧化壓力之影響
論文名稱(外文):Occupational Exposure in Relation to Oxidative DNA Damage in a Glasswork Employee
指導教授:林澤聖林澤聖引用關係
指導教授(外文):Tser-Sheng Lin
學位類別:碩士
校院名稱:國立聯合大學
系所名稱:環境與安全衛生工程學系碩士班
學門:工程學門
學類:環境工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:55
中文關鍵詞:氧化壓力8-OHdG玻璃製造工
外文關鍵詞:oxidative stress8-OHdG
相關次數:
  • 被引用被引用:3
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研究勞工於在物理性危害因子及重金屬之環境下工作,對勞工體內DNA損傷程度之影響。以尿液中8-羥基去氧鳥糞核糖(8-OHdG)之濃度作為生物指標,來量化人體的DNA損傷之程度。依照勞工健康保護規則之規定,將受試員工分為高溫、噪音、粉塵、高溫+噪音、粉塵+噪音與辦公室一般員工等六組,以一般組作為對照組與其他組別作比較。由於工廠的生產線員工以男性佔大多數,為了避免性別的差異所造成的影響,只選取男性員工作為研究對象。收集每位員工的尿液樣本,分析尿液中8-OHdG濃度及各種重金屬之濃度。並且發問卷收集個人基本資料。分析結果發現在“高溫”組的員工體內氧化傷害高於對照組,分別為27.51±41.15 vs. 7.94±10.51 (μg/g creatinine) (mean±SD),表示在高溫的工作環境中會增加員工的氧化壓力。而年齡、抽菸習慣與輪班狀況都不會影響尿液中8-OHdG的濃度。勞工尿液中重金屬濃度與8-OHdG濃度的等級相關分析發現,“高溫”中除了錳沒有達顯著外,其他重金屬與8-OHdG濃度的相關性均有達到統計上的顯著意義,其中相關係數大於0.7的金屬有鎘、銅、硒、鋅;“高溫+噪音”中有達顯著意義的金屬有鎘、汞、錳、鉈。而其他組別重金屬與8-OHdG濃度的相關性都不高,表示員工暴露在高溫的作業環境中,重金屬的濃度與DNA氧化傷害的程度會呈現正相關。進一步以複迴歸及一般線性模式進行分析各變項與8-OHdG濃度間的相關性,發現高溫環境的影響最大,但重金屬的影響會隨著選用的分析模式的不同而有不同的結果。推測因為重金屬毒性會與其溶解度、人體吸收途徑、體內代謝、本身化學反應特性等複雜的機制有關而有所不同,且在多種金屬且高濃度的暴露之下,沒有辦法只單靠體內代謝出的8-OHdG濃度來釐清各金屬對人體的氧化性傷害之程度。本研究評估玻璃製造廠的員工暴露在各種危害下體內DNA氧化傷害的情況,發現高溫環境中對勞工影響甚大,且在高溫環境下重金屬比較容易對勞工造成DNA的氧化傷害。
This study aimed to investigate the influence of occupational stresses on oxidative stress in glass manufacturing workers. The level of urinary 8-hydroxydeoxyguanosine (8-OHdG), a biomarker of oxidative stress, was determined to indicate the intensity of possible oxidative stress of those workers. We classified those workers into six groups according their possible occupational stresses as following: thermal, noise, dust, thermal & noise, dust & noise, and unexposed workers. Because the majority of factory workers are men, to avoid the effect of gender. Therefore, only male employees selected for the study. Analysis of urinary 8-OHdG concentration and the concentration of various heavy metals, and self-reported questionnaires were collected to obtain their personal information. The 8-OHdG levels of control group, thermal group were 27.51±41.15 vs. 7.94±10.51 (μg/g creatinine) (mean±SD), respectively. That the working environment at high temperature will increase their oxidative stress. 8-OHdG levels did not vary according to age, smoking and shift. The association between 8-OHdG levels and concentration of heavy metals by Spearman rank correlation, In addition to manganese, concentration of heavy metals also were positively associated with 8-OHdG levels in thermal group. the concentration of 8-OHdG is not statistically significant in the other group. Employees exposed to the work of high-temperature environment, metal concentration found to be significantly associated with urinary 8-OHdG concentrations. Specific differences in the toxicities of metal ions may be related to differences in solubility, absorbability, transport, chemical reactivity, Exposure to high concentrations of heavy metals, 8-OHdG levels in urine should not be regarded as a marker of exposure to heavy metals. In conclusion, high-temperature working environment will increase the staff of oxidative stress, and this will increase the oxidative stress of heavy metals on the DNA.
第一章 前言.................................................................................................................1
第一節 研究背景..........................................................................................................1
第二節 研究目的..........................................................................................................2
第三節 研究假設..........................................................................................................2
第二章 文獻探討.........................................................................................................3
第一節 氧化傷害定義..................................................................................................3
第二節 生物性指標8-OHdG........................................................................................3
第三節 熱對人體可能造成的危害..............................................................................4
第四節 硒對人體的影響..............................................................................................5
第五節 影響8-OHdG的可能因素................................................................................7
第六節 職業上可能存在的氧化傷害........................................................................11
第三章 材料與方法...................................................................................................13
第一節 研究對象........................................................................................................13
第二節 問卷調查........................................................................................................13
第三節 尿液樣本採集及貯存....................................................................................14
第四節 尿液中8-OHdG分析....................................................................................14
第五節 尿液中肌酸酐測定........................................................................................15
第六節 尿液中重金屬濃度測定................................................................................15
第七節 資料整理與統計分析....................................................................................15
第四章 結果................................................................................................................20
第一節 員工之基本資料特性....................................................................................20
第二節 尿液中8-OHdG分析結果............................................................................24
第三節 員工尿液中重金屬濃度分析結果................................................................26
第四節 8-OHdG多變項分析.....................................................................................32
第五章 討論................................................................................................................37
第一節影響8-OHdG因素之探討..............................................................................37
第二節重金屬對氧化傷害的影響..............................................................................38
第六章結論與建議......................................................................................................40
參考文獻......................................................................................................................41
附件..............................................................................................................................48
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