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研究生:張雅欣
研究生(外文):Ya-Xin Zhang
論文名稱:市售不同蜂花粉產品之抗氧化活性特性分析
論文名稱(外文):Antioxidant Properties of Different Bee Pollen Products
指導教授:彭及忠
指導教授(外文):Chi-Chung Peng
學位類別:碩士
校院名稱:國立虎尾科技大學
系所名稱:光電與材料科技研究所
學門:工程學門
學類:電資工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:61
中文關鍵詞:蜂花粉抗氧化活性
外文關鍵詞:Bee pollenAntioxidant activity
相關次數:
  • 被引用被引用:2
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  • 收藏至我的研究室書目清單書目收藏:1
隨著保健養生的意識抬頭,人們於利用蜜蜂相關產品的基礎上,逐漸地瞭解且接觸並開始應用蜂花粉。蜂花粉中的多酚及黃酮類化合物提供了良好的抗氧化活性,對人類的健康有很大的益處,可減少人體被自由基攻擊。蜂花粉的成分及香氣亦會因花粉來源不同而有很大的差異,本研究將針對常見的幾種蜂花粉產品進行抗氧化活性測試並比較之間的差異性。在此分別針對油菜花粉、百草花粉、蒲鹽花粉、茶花粉、松花粉、花粉錠、含羞草花粉、混合型花粉、多種花粉源花粉等九種不同蜂花粉產品進行抗氧化測試包含清除DPPH自由基能力、清除氫氧自由基能力、清除超氧自由基能力、抑制亞麻油酸能力及還原力等抗氧化活性方法測試,並分析其總蛋白質含量以及利用磷鉬酸酚試劑(Folin-Ciocalteu reagent)分析法量測其總酚類含量。結果顯示,不同蜂花粉產品因花粉源及製程或加工不同,總酚類含量即不同,進而影響了抗氧化活性之表現。其中以油菜花粉、百草花粉、蒲鹽花粉及花粉錠於多項抗氧化能力測定下,具有較佳之抗氧化性效能;而多種花粉源花粉產品的抗氧化性能力則較其他蜂花粉產品差。
This study aimed to evaluate the antioxidant properties of different bee pollen products. Nine samples were collected by Fu-Chang Beekeeping in Hualien, Taiwan or bought from the market of Taiwan. Rape pollen product displayed predominant antioxidant properties, including scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and inhibition of linoleic acid peroxidation. However, lower antioxidant activities were observed in the mixed bee pollen product for the assay of DPPH radicals, inhibition of linoleic acid peroxidation, scavenging activity of superoxide anion radicals and reducing power. In addition, higher contents of proteins were determined in the pollen tablet product and the higher contents of polyphenolic compounds were determined in the wild flower pollen product than others. Many reserch demonstrated that the polyphenolic compounds may be the major component for giving the antioxidant activities in bee pollens. In summary, the bee pollen products from differrent plant species and via processed products did affect the antioxidant potencies, and rape pollen product showed the most substantial antioxidant activities.
中文摘要......i
Abstract......ii
誌謝......iii
目錄......iv
表目錄......viii
圖目錄......ix
第一章 前言......1
第二章 文獻整理......2
2.1 蜂花粉簡介......2
2.2 自由基簡介......3
2.3 人體自由基之種類......4
2.3.1 活性氧......4
2.3.2 活性氮......7
2.4 脂質氧化之作用......8
2.4.1 自氧化作用......9
2.4.2 熱引發的氧化作用......10
2.4.3 光感應的氧化作用......10
2.4.4 酵素催化的氧化作用......11
2.5 自由基與人體健康之關係......11
2.5.1 自由基對蛋白質的傷害......12
2.5.2 自由基對脂質的傷害......12
2.5.3 自由基對DNA的傷害......12
2.6 人體抗氧化防禦系統......13
2.6.1 酵素型抗氧化防禦系統......13
2.6.2 非酵素型抗氧化防禦系統......15
2.7 天然抗氧化劑......17
2.7.1 維生素C......17
2.7.2 維生素E......17
2.7.3 麩胱甘肽......18
2.7.4 輔酶Q......18
2.7.5 尿酸......18
2.7.6 多酚類與類黃酮......19
2.8 抗氧化活性測定之原理......19
2.8.1 DPPH自由基清除能力之測定......20
2.8.2 氫氧自由基清除能力測定......20
2.8.3 超氧自由基清除能力測定......21
2.8.4 抑制亞麻油酸氧化能力測定......21
2.8.5 還原力測定......22
2.9 相關專利檢索......23
2.9.1抗氧化活性測定方法之相關專利......23
2.9.2天然抗氧化物質應用之相關專利......24
第三章 材料與方法......27
3.1材料......27
3.2實驗方法......27
3.2.1 花粉樣品製備......27
3.2.2 花粉蛋白質含量測定......28
3.2.3 花粉總酚含量測定......28
3.2.4 DPPH自由基清除能力測定......29
3.2.5 氫氧自由基清除能力測定......29
3.2.6 超氧自由基清除能力測定......30
3.2.7 抑制亞麻油酸氧化能力測定......30
3.2.8 還原力測定......31
3.2.9 統計分析......32
第四章 結果與討論......33
4.1 花粉蛋白質含量測定......33
4.2 總酚含量測定......33
4.3 DPPH自由基清除能力測定......34
4.4 氫氧自由基清除能力測定......35
4.5 超氧自由基清除能力測定......35
4.6 抑制亞麻油酸氧化能力測定......36
4.7 還原力測定......36
第五章 結論......37
參考文獻......40
附錄......47
英文論文大綱......55
個人簡歷......61
[1] Yamaguchi P. (2006) Japan’s nutraceuticals today–Functional foods Japan. NPIcenter Web site. http://www.npicenter.com/anm/templates/newsATemp.aspx?articleid_15160&zoneid_45.
[2] Hilliam M. (2000) Functional food: How big is the market? World of Food Ingredients, 12, 50–53.
[3] Royden B. (1994) Bee Pollen: The Perfect Food. The Arthritis trust of America, 1-6.
[4] Liviu A. M., Oltica G. S., Daniel S. D., Otilia B., Olimpia P., Stefan B., Maria G. C. (2009) In vitro antioxidant capacity of honeybee-collected pollen of selected floral origin harvested from Romania. Food Chem., 115, 878–883.
[5] Leja M., Mareczek A., Wyzgolik G., Klepacz B. J., Czekon′ska K. (2007) Antioxidative properties of bee pollen in selected plant species. Food Chem., 100, 237–240.
[6] Blaise W. L., Owen K. D., Stephen B., Anthony D., Thomas D. (2009) Antioxidant activity of Sonoran Desert bee pollen. Food Chemistry, 115, 1299–1305.
[7] Tania M. S. S., Celso A. C., Antonio C. S. L., Jos′e M. B. F., Eva M. S. S., Breno M. F., Francisco de A. R. S. (2006) Chemical composition and free radical scavenging activity of pollen loads from stingless bee Melipona subnitida Ducke. Food Compost. Anal., 19, 507–511.
[8] 安奎,何鎧光,陳裕文。(2004)。養蜂學。國立編譯館。
[9] 孫安迪。(1999)。以醫學保健觀點論蜂產品。台灣養蜂業展望研討會,第1-19頁。
[10] McNally J. B., McCaughey W. F., Standifer L. N, Todd F. E. (1965) Partition of excreted nitrogen from honey bees fed various proteins. J Nutr, 85, 113-116.
[11] Tomas L. F., Garciagrau M. M., Nieto J. L., Tomas B. F. A. (1992) Flavonoids from Cistus-Ladanifer bee pollen. Phytochemistry, 31, 2027–2029.
[12] 李錦楓。(2002)。蜂產品的保件功能(四) 。健康世界,第199期,第41-42頁。
[13] Halliwell G. (1990) Role of free radicals and catalytic metal ions in human disease: an overview. Methods Enzymol, 186, 1-85.
[14] Halliwell B., Gutteridge J. M. C., Cross C. E. (1992) Free radicals, antioxidants and human disease: where are we now? J. Lab. Clin. Med., 119, 598-620.
[15] Chen H. Y., Yen G. C. (1998) Free Radicals, Antioxidant Defenses and Human Health. Nutrition Sciences Jourmal, 23, 105-121.
[16] Halliwell B. (1994) Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet., 10, 334-702.
[17] 施益民,呂鋒洲。(1989)。自由基與各種疾病。當代醫學,第16期,第399-407頁。
[18] Schechter S. R. (1996) Fighting free radicals with antioxidant. Health Foods Business, 6, 28-29.
[19] Cacciuttolo M. A., Trinh L., Lumpkin J. A., Rao G. (1993) Hyperoxia induces DNA damage in mammalian cells. Free Radic Biol Med, 14, 267-76.
[20] 趙克然,楊毅軍,曹道俊。(2003)。氧自由基與臨床。台北市合記出版社。
[21] Lander H. M. (1997) An essential role for free radicals and derived species in signal transduction. FASEB J., 11, 118-124.
[22] Green M. J., Hill H. A. (1984) Meth. Enzymol., 105, 3-22.
[23] Halliwell B. (2006) Oxidative stress and neurodegeneration: where are we now? J. Neurochem., 97, 1634-1658.
[24] Halliwell B. (1997) Antioxidants and human diseases: a general introduction. Nutr. Rev., 55, 44-52.
[25] Kohen R., Nyska A. (2002) Oxidation of biological systems: oxidative stress phenomena, antioxidant, redox reactions, and methods for their quantification. Toxicol. Pathol., 30, 620-650.
[26] Diplock A. (1991) Antioxidant nutrients and disease prevention: an overview. Am. J. Clin. Nutr., 53, 189-193.
[27] Cheeseman K. H. (1993) Mechanisms and effects of lipid peroxidation. Mol. Aspects Med., 14, 191-7.
[28] Diplock A. T., Charleux J. L., Grozier w. G., Jok F. J., Rice E. C., Roberfroid M., Stahl W., Vina R. J. (1998) Functional food science and defense against reactive oxidative species. Br. J. Nutr., 80, 77-112.
[29] Draper H. H., Agarwal S., Nelson D. E. V., Wee J. J., Ghoshal A. K., farber E. (1995) Effects of peroxidative stresss and age on the concentration of a deoxyguanosine-malondialdehyde adduct in rat DNA. Lipids, 30, 959-961.
[30] Marnett L. J., Burcham P. C. (1993) Endogenous DNA adducts: potentiall and paradox. Chem. Res. Toxicol., 6, 771-785.
[31] Beckman J. S., Beckman T. W., Chen J., Marshall P.A., Freeman B.A. (1990) Apparent hydroxyl production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc. Natl. Acad. Sci. USA, 87, 1620-1624.
[32] Namiki M. (1990) Antioxidants/antimutagens in foods. Crit. Rev. Food Sci. Nutr., 29, 273-300.
[33] Brown G.C., Borutaite V. (2002) Nitric oxide inhibition of mitochondrial respiration and its role in cell death. Free Radi. Biol. Med., 11, 1440-1450.
[34] Radi R., Cassina R., Hodara R., Quijano R. Castro L. (2002) Peroxynitrite reactions and formation in mitochondria. Free Radic. Biol. Med., 11, 1451-1464.
[35] Kubow S. (1990) Toxicity of dietary lipid peroxidation products. Trend in Food Sci. Tech., 67-70.
[36] Thomas M. J. (1995) The role of free radicals and antioxidants: how do we know that they are working? Crit. Rev. Food Sci. Nutr., 35, 21-39.
[37] Nestel P. J. (1995) The role of antioxidants in preventing coronary disease. Suppl. Food Aust., 47, 28-29.
[38] Mistry B. S., Min D. B. (1992) Oxidized flavor compounds in edible oils. Off-Flavors in Foods and Beverages, Edited by Charalambous G., 171-209.
[39] Melton S. L. (1983) Methodology for following lipid oxidation in muscle foods. Food Technol., 37, 105.
[40] Fennema O. R. (1996) Lipids in “Food Chemistry” 3rd ed. Marcel Dekker Inc.211-213.
[41] Frankel E. N. (1984) Lipid oxidation: mechanism, products and biological significance. J. Am. Oil Chem. Soc., 61, 1908-1916.
[42] Kanner J., German J. B., Kinsella J. E. (1987) Initiation of lipid peroxidation in biological systems. Crit. Rev. Food Sci. Nutr., 25, 317-363.
[43] Halliwell B., Gutteridge J. M. C. (1989) Free radicals, aging and disease. J. Free Radic. Biol. Med., 484-487.
[44] Stadtman E. R. (1992) Protein oxidation and aging. Science, 257, 1220-1224.
[45] Davies K. J. A. (1987) Protein damage and degradation by oxygen radicals. J. Biol. Chem., 262, 9895-9901.
[46] Frankel E. N. (1991) Recent advances in lipid oxidation. J. Sci. Food Agric., 54, 495-511.
[47] Sterbauer H., Cheeseman K. H. (1990) Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol, 186, 407-417.
[48] Gutteridge J. M. C., Halliwell B. (1990) The measurement and mechanism of lipid peroxidation in biological systems. Trends Biochem. Sci., 15, 129-135.
[49] Aruoma O. I., Halliwell B., Gajewski E., Dizdaroglu M. (1989) Damage to the bases in DNA induced by hydrogen peroxide and ferric ion chelates. J. Biol. Chem., 264, 20509-20512.
[50] Saul R. L., Ames B. N. (1986) Background levels of DNA damage in the population. Basic Life Sci., 38, 529-535.
[51] Cheng K. C., Cahill D. S., Kasai H., Nishimura S., Loeb. L. A. (1992) 8-hydroxyguanine, an abundant form of oxidative DNA damage, causes GT and AC substitution. J. Biol. Chem., 267, 166-172.
[52] Giese J. (1996) Antioxidants: Tool for preventing lipid oxidation. Food Technol., 11, 73-82.
[53] Shahidi F., Wanasundara P. K. (1992) Phenolic antioxidants. CRC Crit. Rev. Food Sci. Nutr., 32, 67-103.
[54] Kochnar S. P., Rossel J. B. (1990) Detection, estimation and evaluation of antioxidants in food systems. Food antioxidant, 19-64.
[55] Kellogg E. W., Fridovich I. (1975) Superoxide, hydrogen peroxide, and singlet oxygen in lipid peroxidation by a xanthine oxidase system. J. Biol Chem., 250, 8812-8817.
[56] Pastore A., Federici G., Bertini E., Piemonte F. (2003) Analysis of glutathione: implication in redox and detoxification. Clin. Chim. Acta., 333, 19-39.
[57] Cadenas E. (1995) Mechanisms of oxygen activation and reactive oxygen species detoxification. Oxidative Stress and Antioxidant Defenses in Biology. Ahmad, S. Chapman and Hall, Eds., International Thomaon Publishing Inc., New York. pp. 1-25.
[58] Folkers K., Osterborg A., Wu Z. F. (1997) Activities of vitamin Q10 in animal models and a serious deficiency in patients with cancer. Biochem. Biophys. Res. Commun., 234, 296-299.
[59] Kawamukai M. (2002) Biosynthesis, bioproduction and novel roles of ubiquinone. J. Biosci. Bioeng., 94, 511-517.
[60] Becker B. F. (1993) Towards the physiological function of uric acid. Free Radic. Biol. Med., 14, 615-631.
[61] Cook N. C., Samman S. (1996) Flavonoids-Chemistry, metabolism, cardioprotective effects, and dietary source. J Nutr Biochem., 7, 66-76.
[62] Williams W. B., Cuvelier M. E., Berset C. (1995) Use of a free radical method to evaluate antioxidant activity. Lebensm-Wiss. Technol., 28, 25-30.
[63] Halliwell B., Gutteridge J. M., Aruoma O. I. (1987) The deoxyribose method: a simple "test-tube" assay for determination of rate constants for reactions of hydroxyl radicals. Anal. Biochem., 165, 215-219.
[64] Rock J., Gryglewski R. I. (1988) Flavonoids are scavengers of superoxide anions. Biochem. Pharma., 37, 837-841.
[65] Yen G. C., Duh P. D., Chuang D. Y. (2000) Antioxidant activity of anthraquinones and anthrone. Food Chem., 70, 437–441.
[66] Oyaizu M. (1986) Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucoseamine. Jpn. J. Nutr., 44, 307–315.
[67] Liu J. R., Yang Y. C., Shi L. S., Peng C. C. (2008) Antioxidant properties of royal jelly associated with larval age and time of harvest J. Agric. Food Chem., 56, 11447-11452.
[68] Slinkard K., Singleton V. L. (1977) Total phenol analysis. Am. J. Enol. Vitic., 28, 49–55.
[69] Nagai T., Inoue R., Suzuki N., Nagashima T. (2006) Antioxidant properties of enzymatic hydrolysates from royal jelly. J. Med. Food, 9, 363–367.
[70] Okezie I. A. (1998) Free Radicals, Oxidative Stress, and Antioxidants in Human Health and Disease. JAOCS, 75, 199-212.
[71] Martijn B K. (2004) Health claims for functional foods. BMJ., 328, 180-181.
[72] 白樹民,高愛新,雷浪偉,李培荣,李江毅,鄭珍,楊小明。(2006)。松花粉對慢性應激大鼠腸道微生態的影響。航天醫學與醫學工程。第3期,第189-193頁。
[73] 張荣標,何聆,陳潤,林健,林蔚。(2005)。油菜蜂花粉對正常小鼠免疫功能的影響。實用預防醫學。第1期,第44-46頁。
[74] 王秀玲。(2007)。花粉的食用,藥用價值及開發前景。第20期,第6233-6234頁。
[75] Kahl R., Kappus H., (1993) Toxicity of synthetic antioxidants BHA and BHT in comparison with natural antioxidants vitamin E. Zeitschrift fur Lebensmittel-Untersuchungund–Forschung, 196, 329–338.
[76] Gurdip S., Sumitra M., DeLampasona M. P., Cesar A. N. C. (2007) A comparison of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents. Food and Chemical Toxicology, 45, 1650-1661.
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