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研究生:洪維廷
研究生(外文):Wei-Ting Hung
論文名稱:Furosemide與水的服用對於人體尿液中metandienone代謝物的影響
論文名稱(外文):Effects of furosemide and water consumption on metandienone metabolites in human urine
指導教授:邱鐵雄邱鐵雄引用關係
指導教授(外文):Chiu,TED H
學位類別:碩士
校院名稱:慈濟大學
系所名稱:藥理暨毒理學研究所
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
畢業學年度:97
語文別:中文
論文頁數:66
中文關鍵詞:類固醇代謝利尿劑氣相層析
外文關鍵詞:metandienonefurosemidemetaboliteGC/MS
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Metandienone (17��-hydroxy-17��-methylandrosta-1,4-dien-3-one )是一種合成的外生性同化類固醇(anabolic steroid),其在人體中產生的代謝物有5種,可以經尿液檢測。Furosemide是一種強效的環利尿劑(loop diuretics)。有些運動員因為使用了禁藥,而想利用利尿劑幫助迅速地將藥物排出體外,企圖逃避被檢測出,因而造成偽陰性之出現。本研究的目的是要探討服用利尿劑以及大量的水之後,是否會影響metandienone的代謝情況。
實驗設計上,5位健康的男性受試者,進行4次不同的人體實驗,4次實驗分別為:1-服用metandienone(10 mg);2-服用metandienone(10 mg)及併服furosemide(40 mg);3-服用metandienone(10 mg)及併服大量水分(400 ml/hr);4-服用metandienone(10 mg)及併服furosemide(40 mg)及大量水分(400 ml/hr)。收集之尿液以GC-MS分析,探討3種metandienone代謝物在尿液中濃度與不同時間點之變化及代謝累積量。
本研究結果顯示:1. 服用10 mg metandienone後第二小時就可以在尿液中分析出3種代謝物:6b-OH-metandienone (AM3M2)、17α-methyl-5b-androstane-3α,17b-diol (AM3M4b)和17,17-dimethyl-18-norandrosta-1,4,13 (14)-trien-3-one (AM3M6)。2. 合併服用 10 mg metandienone和40 mg furosemide可以有效的降低3種代謝物尿液中濃度。3. 服用10 mg metandienone並喝400 mL/hr的水後,對降低尿液中3種代謝物濃度影響更明顯。4. 合併服用 10 mg metandienone和40 mg furosemide,再喝400 mL/hr的水後,可以有效降低尿液中3種代謝物濃度,也可以延遲3種代謝物出現的時間。
Metandienone is a synthetic anabolic androgenic steroid. There are at least five metandienone metabolites can be detected in human urine. Furosemide is a potent loop diuretic. Some athletes use diuretics to speed up the excretion of prohibited substances, so that they may pass drug tests. The purpose of this study was to investigate the impacts of furosemide and/or large amount of water on the metabolism of metandienone. Five healthy males were recruited to participate in four separate experiments. Following experimental conditions were designed: Experiment 1 – taking metandienone (10 mg) alone; Experiment 2 – ingesting metandienone (10 mg) and furosemide (40 mg); Experiment 3 – consuming metandienone (10 mg) and water (400 ml/hr); and Experiment 4 – consuming metandienone (10 mg), water (400 ml/hr) and furosemide (40 mg). Urine specimens were collected and analyzed using GC-MS. Concentrations of the urinary metabolites and their accumulated amounts throughout the study were quantified. The following results were obtained: 1. 6b-OH-metandienone (AM3M2), 17α-methyl-5b-androstane-3α,17b-diol (AM3M4b) and 17,17-dimethyl-18-norandrosta-1,4,13 (14)-trien-3-one (AM3M6) were found in urine as soon as two hours following metandienone administration; 2. Administration of furosemide alone reduced the urinary concentrations of the three metabolites in urine; 3. Consumption of large amount of water appeared to be more effective in reducing the urine metabolite concentrations than that of furosemide administration; 4. The combination of water drinking and furosemide ingestion not only enhanced effects in reducing concentrations of the three metabolites in urine, but also delayed their excretion times.
目錄(Contents)

1、背景介紹 (Background) 1
1.1 運動與禁藥(Background) 1
1.2合成類固醇(Anabolic steroids) 1
1.3 Metandienone 3
1.4利尿劑(Diuretics) 5
2、研究目的(Purpose) 8
2.1 目標 (Specific aims) 8
3、實驗 (Experiments) 9
3.1 受測者 (Subjects and drug dose) 9
3.2 實驗設計(Experimental design) 9
3.3 材料(Materials) 11
3.4 實驗方法 (Methods) 13
4、結果(Results) 17
4.1 Metandienone代謝物在尿液中的鑑定(Identification of metandienone metabolites in urine) 17
4.2偵測極限與定量極限 (Limit of detection and Limit of quantification) 20
4.3檢量線(Calibration curve) 20
4.4回收率(Recovery) 23
4.5精確度(Precision)與準確度(Accuracy) 24
4.6尿液體積(Urine volume) 25
4.7 AM3M2、AM3M4b以及AM3M6尿液中濃度變化(Metabolic profiles of AM3M2, AM3M4b and AM3M6 in urine samples) 27
4.8 AM3M2、AM3M4b以及AM3M6代謝情形(Cumulation amount of AM3M2, AM3M4b and AM3M6 in urine samples) 33
4.9AM3M2、AM3M4b以及AM3M6尿液中代謝累積量(Accumulated excreted amount of AM3M2, AM3M4b and AM3M6 in urine samples) 44
5、討論(Discussion) 55
6、結論(Conclusion) 60
7、參考文獻(References) 61
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