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研究生:許毓玲
研究生(外文):Yu-Ling Hsu
論文名稱:潛在抑制對大鼠杏仁核和前扣帶迴皮質神經元活動的影響
論文名稱(外文):The effect of latent inhibition on the neuronal activities ofamygdala and anterior cingulate cortex in rat
指導教授:郭昶志郭昶志引用關係
指導教授(外文):Chung-Chih kuo
學位類別:碩士
校院名稱:慈濟大學
系所名稱:神經科學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
畢業學年度:97
語文別:中文
論文頁數:60
中文關鍵詞:杏仁核前扣帶迴皮質抑制型躲避作業單一神經元潛在抑制
外文關鍵詞:amygdalaanterior cingulate cortexinhibitory avoidance learningsingle
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情緒是記憶形成過程中的一個重要因子,它會強化記憶的形成,但也會受到
其它因子的影響,例如環境的前曝露會引起潛在抑制的現象,干擾情緒記憶的表
現。先前的研究指出杏仁核參與學習與記憶的歷程,而前扣帶迴皮質在潛在抑制
現象中則扮演重要角色。本論文主要探討在前曝露後經歷抑制型躲避學習作業,
對杏仁核和前扣帶迴神經元的影響。用微電極線插入杏仁核和前扣帶迴皮質,作
為記錄電極。在清醒大鼠經歷前曝露及抑制型躲避學習作業時,以細胞外記錄的
方式全程記錄杏仁核和前扣帶迴神經元活動的變化。實驗組的大鼠在訓練前會在
穿梭箱接受5天的前曝露,對照組則是在另一個與穿梭箱不同的作業箱接受前曝
露,並於第6天在穿梭箱中進行訓練前測試,之後接受抑制型躲避學習作業,在
暗箱給予 0.4 毫安培 1 秒的足部電擊,隔天接受訓練後測試。行為的結果顯示
不論是實驗組或對照組在經過抑制型躲避學習作業之後,進入暗箱的時間都比訓
練前增加,顯示大鼠學習了此作業,且實驗組進入暗箱的時間較對照組短,代表
環境的前曝露引發潛在抑制的現象。觀察整個實驗過程實驗組和對照組的杏仁核
和前扣帶迴整體神經元活動,在經過抑制型躲避學習作業之後,當大鼠進入穿梭
箱,杏仁核神經元和前扣帶迴神經元活動都有上升的情形;而比較實驗組和對照
組大鼠,實驗組大鼠杏仁核神經元活動的變化顯著比對照組低,而實驗組前扣帶
迴神經元活動的變化則明顯比對照組高。由以上結果顯示環境前曝露會引發潛在
抑制的現象,而在這個過程中會影響杏仁核和前扣帶迴皮質的神經元活動,因此
推論杏仁核和前扣帶迴皮質可能參與潛在抑制現象對恐懼情緒記憶的調控。
Emotion can have a powerful impact on memory, and can strengthen the
formation of memory. Emotional memory can be affected by many factors. For
example, pre-exposure of context may induced the phenomenon of latent inhibition to
interfere the emotional memory. The amygdala (AMY) involvs in the learning and
memory processes of emotion events and anterior cingulate cortex (ACC) plays an
important role in the latent inhibition. This study examined how the pre-exposure of
context affected the neuronal activities of AMY and ACC in the inhibitory avoidance
task. The micro-wire electrodes were chronically implanted in the AMY and ACC of
Long-Evans rats. The neuronal activities of AMY and ACC in conscious rats were
recorded extracellularly during the pre-exposure of context and inhibitory avoidance
(IA) training. The animals of pre-exposure (PE) group were exposed to the context of
IA for five days before training. The animals of non-pre-exposure (NPE) group were
exposed to the context different from the shuttle box. On the sixth day, the rats was
tested before training and then received the foot-shock (0.4 mA , 1s) in the dark side
of apparatus. The rats were tested on the next day of foot shock training. The retention
latencies of both PE and NPE groups were enhanced after the IA training. However,
shorter retention latency was shown in the PE group than the NPE group. These
results suggested that the rats learned the IA task and pre-exposure of context induced
latent inhibition (LI) effect. Multiple single-unit activities of AMY and ACC in both
groups were recorded simultaneously during the pre-exposure period and IA task.
After training, the unit activities of AMY and ACC were change. The change of AMY
unit activities in the PE group was less then the one in the NPE group. The change of
ACC unit activities in the PE group was more then the one in the NPE group. These
results showed that the process of context pre-exposure induced the latent inhibition
in IA paradigm and the neuronal activities of AMY and ACC were affected in the
phenomenon of latent inhibition. It has been suggested that AMY and ACC might be
involved in the modulation of fear emotion and memory.
致謝............................................................................................................................... I
中文摘要....................................................................................................................... II
英文摘要......................................................................................................................IV
目錄..............................................................................................................................VI
第一章 緒論............................................................................................................1
1.1 恐懼情緒記憶 (Fear emotional memory) .....................................................1
1.2 制約(Conditioning) ...................................................................................2
1.3 杏仁核 (Amygdala) .......................................................................................3
1.4 前扣帶迴皮質(Anterior cingulate cortex)...................................................6
1.5 潛在抑制(Latent Inhibition)....................................................................11
1.6 研究目的與假說...........................................................................................13
第二章 材料與方法....................................................................................................14
2.1 實驗動物.......................................................................................................14
2.2 植入電極手術...............................................................................................14
2.3 神經元電訊號的記錄...................................................................................15
2.4 行為訓練與檢測...........................................................................................16
2.4.1 實驗儀器............................................................................................16
2.4.2 行為操作過程....................................................................................17
2.5 灌流與腦組織染色.......................................................................................18
2.6 資料分析.......................................................................................................18
2.6.1 抑制型躲避學習作業行為分析........................................................18
2.6.2 神經元活動分析................................................................................19
第三章 研究結果........................................................................................................22
3.1 環境前曝露對抑制型躲避學習作業行為的影響.......................................22
3.2 杏仁核和前扣帶迴皮質在抑制型躲避學習作業之神經元的變化...........23
3.3 整個實驗過程杏仁核和前扣帶迴皮質神經元活動的變化.......................26
第四章 綜合討論........................................................................................................29
4.1 潛在抑制造成抑制型躲避學習作業行為的改變.......................................29
4.2 潛在抑制現象中杏仁核神經元活動的反應...............................................31
4.3 潛在抑制現象中前扣帶迴皮質神經元活動的反應...................................33
參考文獻......................................................................................................................36
圖表說明......................................................................................................................42
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