臺灣博碩士論文加值系統

古柯鹼與安非他命調控轉錄因子胜肽 (Cocaine- and amphetamine- regulated transcript peptide, CARTp)廣泛的分布在中樞神經系統。被認為可做為神經調控者或者是神經傳訊者以參與許多神經生理反應；諸如藥物成癮、飲食調控、內分泌調控、焦慮等。前腹外側延腦 (rostral ventrolateral medulla, RVLM) 為中樞興奮交感神經之核區；已知在持續性與反射性交感神經血壓調節作用中扮演重要角色。解剖學上的證據指出位於RVLM的神經元及神經纖維含有大量CARTp。
這些結果暗示了CARTp可能參與RVLM的心血管功能調控。有研究指出投與CARTp到孤立束核(nucleus tractus solitaries, NTS) 雖無法使血壓上升卻可以減弱phenylephrine引起的心搏徐緩，而此作用可被CARTp之抗體阻斷。其他研究則指出將更高濃度的CARTp投與至中樞，即可造成血壓顯著上升。麩胺酸 (glutamate) 是這些中樞交感神經相關之神經核主要調控心血管功能的神經傳導物質；許多研究指出，磷酸化RVLM上離子型通道型麩胺酸受體－NMDA受體 (N-methy-D-asparate receptors, NMDARs) ，可增強該受體功能，進而增加中樞交感活性之輸出。我們的研究發現經由雙側RVLM投與CARTp (6, 30或60 pmol)可劑量依賴性升高血壓及降低感壓反射敏感度。此外，以側腦室處注射CARTp(20 pmol或2 nmol)， RVLM區NMDA受體次單位NR1上的磷酸化serine 897蛋白質表現量增加，而此磷酸化作用是經由A型蛋白激酶 (protein kinase A, PKA)路徑所作用。研究結果顯示CARTp可能藉由直接作用於RVLM引發升壓反應或藉由間接降低此區之感壓反射敏感度參與中樞之心血管調節，而PKA訊息路徑也許與此反應有關。

CARTp(Cocaine- and Amphetamine- regulated transcript peptide) is widely distributed in CNS as neurotransmitter or neuroregulator involved in many physiological process like addiction, food intake, endocrine and anxiety. The rostral ventrolateral medulla (RVLM) is critical to the tonic and reflexive regulation of arterial blood pressure. CARTp-immunoreactivity was detected in the neurons and nerve terminals in the RVLM. These evidence indicated CARTp may involve in RVLM regulated and increase blood pressure. Previous studies shows bilateral intra-NTS of CARTp (55-102, 0.1-3 nmol) could not change blood pressure but attenuated phenyleprine- induced bradycardia and reversed by CART-antibodies. The other studies shows higher dose CARTp injection into CNS could increase blood pressure. Results from many studies have revealed that phosphorylation of the ionotropic glutamate receptor- NMDA receptors (NMDARs) on the RVLM will increase the receptor function which leads to an increase in central sympathetic outflow. Our results found that bilateral microinjection of CARTp(6 30 60 pmol) into the RVLM decreased the baroreflex sensitivity (BRS) and increased the mean blood pressure. This study implied that CARTp may participate in the central cardiovascular regulation by direct pressor effects and indirect attenuation of the BRS. In addition, CARTp(20 pmol, 2 nmol) injection by intracerebroventricular (i.c.v.) induced a significant increase in the expression of the phosphoserine 897 protein (regulated by PKA) on NR1 subunit in the RVLM. Our results display CARTp may affect RVLM directly in pressor reponses, and indirectly affects in attenuated BRS to regulated central cardiovascular function. In addicition, the PKA signaling pathway may involved in these mediated functions.

中文摘要 1
英文摘要 2
壹、背景介紹 3
貳、研究目的與實驗設計 17
参、實驗方法材料與詳細步驟 21
肆、結果 25
伍、討論 29
陸、結論 33
柒、參考文獻 34
捌、結果附圖 44
附圖一 52
附錄一 53

Adams, LD, Gong, W, Vechia, SD, Hunter, RG, Kuhar, MJ (1999) CART: from gene to function. Brain Res 848(1-2): 137-140.

Albensi, BC (2007) The NMDA receptor/ion channel complex: a drug target for modulating synaptic plasticity and excitotoxicity. Curr Pharm Des 13(31): 3185-3194.

Allen, GV, Cechetto, DF (1992) Functional and anatomical organization of cardiovascular pressor and depressor sites in the lateral hypothalamic area: I. Descending projections. J Comp Neurol 315(3): 313-332.

Beaudry, G, Zekki, H, Rouillard, C, Levesque, D (2004) Clozapine and dopamine D3 receptor antisense reduce cocaine- and amphetamine-regulated transcript expression in the rat nucleus accumbens shell. Synapse 51(4): 233-240.

Bjorkqvist, M, Leavitt, BR, Nielsen, JE, Landwehrmeyer, B, Ecker, D, Mulder, H, Brundin, P, Petersen, A (2007) Cocaine- and amphetamine-regulated transcript is increased in Huntington disease. Mov Disord 22(13): 1952-1954.

Brenz Verca, MS, Widmer, DA, Wagner, GC, Dreyer, J (2001) Cocaine-induced expression of the tetraspanin CD81 and its relation to hypothalamic function. Mol Cell Neurosci 17(2): 303-316.

Broberger, C (1999) Hypothalamic cocaine- and amphetamine-regulated transcript (CART) neurons: histochemical relationship to thyrotropin-releasing hormone, melanin-concentrating hormone, orexin/hypocretin and neuropeptide Y. Brain Res 848(1-2): 101-113.

Burman, KJ, Sartor, DM, Verberne, AJ, Llewellyn-Smith, IJ (2004) Cocaine- and amphetamine-regulated transcript in catecholamine and noncatecholamine presympathetic vasomotor neurons of rat rostral ventrolateral medulla. J Comp Neurol 476(1): 19-31.

Cameron, AA, Khan, IA, Westlund, KN, Willis, WD (1995) The efferent projections of the periaqueductal gray in the rat: a Phaseolus vulgaris-leucoagglutinin study. II. Descending projections. J Comp Neurol 351(4): 585-601.

Chaki, S, Kawashima, N, Suzuki, Y, Shimazaki, T, Okuyama, S (2003) Cocaine- and amphetamine-regulated transcript peptide produces anxiety-like behavior in rodents. Eur J Pharmacol 464(1): 49-54.

Chen, L, Huang, LY (1992) Protein kinase C reduces Mg2+ block of NMDA-receptor channels as a mechanism of modulation. Nature 356(6369): 521-523.

Colombari, E, Sato, MA, Cravo, SL, Bergamaschi, CT, Campos, RR, Jr., Lopes, OU (2001) Role of the medulla oblongata in hypertension. Hypertension 38(3 Pt 2): 549-554.

Couceyro, PR, Koylu, EO, Kuhar, MJ (1997) Further studies on the anatomical distribution of CART by in situ hybridization. J Chem Neuroanat 12(4): 229-241.

Cull-Candy, S, Brickley, S, Farrant, M (2001) NMDA receptor subunits: diversity, development and disease. Curr Opin Neurobiol 11(3): 327-335.

Dall Vechia, S, Lambert, PD, Couceyro, PC, Kuhar, MJ, Smith, Y (2000) CART peptide immunoreactivity in the hypothalamus and pituitary in monkeys: analysis of ultrastructural features and synaptic connections in the paraventricular nucleus. J Comp Neurol 416(3): 291-308.

Douglass, J, Daoud, S (1996) Characterization of the human cDNA and genomic DNA encoding CART: a cocaine- and amphetamine-regulated transcript. Gene 169(2): 241-245.

Douglass, J, McKinzie, AA, Couceyro, P (1995) PCR differential display identifies a rat brain mRNA that is transcriptionally regulated by cocaine and amphetamine. J Neurosci 15(3 Pt 2): 2471-2481.

Dun, NJ, Dun, SL, Kwok, EH, Yang, J, Chang, J (2000a) Cocaine- and amphetamine-regulated transcript-immunoreactivity in the rat sympatho-adrenal axis. Neurosci Lett 283(2): 97-100.

Dun, SL, Brailoiu, E, Hsieh, WK, Lai, CC, Yang, J, Chang, JK, Dun, NJ (2007) Expression and activity of cocaine- and amphetamine-regulated transcript peptide(1-39) in the rat. Regul Pept 140(1-2): 47-54.

Dun, SL, Brailoiu, GC, Yang, J, Chang, JK, Dun, NJ (2006) Cocaine- and amphetamine-regulated transcript peptide and sympatho-adrenal axis. Peptides 27(8): 1949-1955.

Dun, SL, Chianca, DA, Jr., Dun, NJ, Yang, J, Chang, JK (2000b) Differential expression of cocaine- and amphetamine-regulated transcript-immunoreactivity in the rat spinal preganglionic nuclei. Neurosci Lett 294(3): 143-146.

Dun, SL, Ng, YK, Brailoiu, GC, Ling, EA, Dun, NJ (2002) Cocaine- and amphetamine-regulated transcript peptide-immunoreactivity in adrenergic C1 neurons projecting to the intermediolateral cell column of the rat. J Chem Neuroanat 23(2): 123-132.

Dylag, T, Kotlinska, J, Rafalski, P, Pachuta, A, Silberring, J (2006) The activity of CART peptide fragments. Peptides 27(8): 1926-1933.

Ekblad, E (2006) CART in the enteric nervous system. Peptides 27(8): 2024-2030.

Elias, CF, Lee, C, Kelly, J, Aschkenasi, C, Ahima, RS, Couceyro, PR, Kuhar, MJ, Saper, CB, Elmquist, JK (1998) Leptin activates hypothalamic CART neurons projecting to the spinal cord. Neuron 21(6): 1375-1385.

Fagergren, P, Hurd, YL (1999) Mesolimbic gender differences in peptide CART mRNA expression: effects of cocaine. Neuroreport 10(16): 3449-3452.

Frankel, RA, Metting, PJ, Britton, SL (1993) Evaluation of spontaneous baroreflex sensitivity in conscious dogs. J Physiol 462: 31-45.

Freeman, WM, Patel, KM, Brucklacher, RM, Lull, ME, Erwin, M, Morgan, D, Roberts, DC, Vrana, KE (2008) Persistent alterations in mesolimbic gene expression with abstinence from cocaine self-administration. Neuropsychopharmacology 33(8): 1807-1817.

Gautvik, KM, de Lecea, L, Gautvik, VT, Danielson, PE, Tranque, P, Dopazo, A, Bloom, FE, Sutcliffe, JG (1996) Overview of the most prevalent hypothalamus-specific mRNAs, as identified by directional tag PCR subtraction. Proc Natl Acad Sci U S A 93(16): 8733-8738.

Guyenet, PG (2006) The sympathetic control of blood pressure. Nat Rev Neurosci 7(5): 335-346.

Hsun Lin, H, Chiu, HY, Lai, CC (2005) Potentiation of spinal N-methyl-D-aspartate-mediated nociceptive transmission by cocaine-regulated and amphetamine-regulated transcript peptide in rats. Neuroreport 16(3): 253-257.

Hubert, GW, Kuhar, MJ (2008) Cocaine administration increases the fraction of CART cells in the rat nucleus accumbens that co-immunostain for c-Fos. Neuropeptides 42(3): 339-343.

Hubert, GW, Kuhar, MJ (2006) Colocalization of CART peptide with prodynorphin and dopamine D1 receptors in the rat nucleus accumbens. Neuropeptides 40(6): 409-415.

Hubert, GW, Kuhar, MJ (2005) Colocalization of CART with substance P but not enkephalin in the rat nucleus accumbens. Brain Res 1050(1-2): 8-14.

Hunter, RG, Jones, D, Vicentic, A, Hue, G, Rye, D, Kuhar, MJ (2006) Regulation of CART mRNA in the rat nucleus accumbens via D3 dopamine receptors. Neuropharmacology 50(7): 858-864.

Hunter, RG, Vicentic, A, Rogge, G, Kuhar, MJ (2005) The effects of cocaine on CART expression in the rat nucleus accumbens: a possible role for corticosterone. Eur J Pharmacol 517(1-2): 45-50.

Hwang, LL, Chen, CT, Li, TL, Chiu, CZ, Chi, SF (2004) Central pressor effects of CART peptides in anesthetized rats. Neuropeptides 38(2-3): 69-76.

Jaworski, JN, Hansen, ST, Kuhar, MJ, Mark, GP (2008) Injection of CART (cocaine- and amphetamine-regulated transcript) peptide into the nucleus accumbens reduces cocaine self-administration in rats. Behav Brain Res 191(2): 266-271.

Jaworski, JN, Kozel, MA, Philpot, KB, Kuhar, MJ (2003) Intra-accumbal injection of CART (cocaine-amphetamine regulated transcript) peptide reduces cocaine-induced locomotor activity. J Pharmacol Exp Ther 307(3): 1038-1044.

Jean, A, Conductier, G, Manrique, C, Bouras, C, Berta, P, Hen, R, Charnay, Y, Bockaert, J, Compan, V (2007) Anorexia induced by activation of serotonin 5-HT4 receptors is mediated by increases in CART in the nucleus accumbens. Proc Natl Acad Sci U S A 104(41): 16335-16340.

Jones, DC, Kuhar, MJ (2006) Cocaine-amphetamine-regulated transcript expression in the rat nucleus accumbens is regulated by adenylyl cyclase and the cyclic adenosine 5'-monophosphate/protein kinase a second messenger system. J Pharmacol Exp Ther 317(1): 454-461.

Kardos, A, Watterich, G, de Menezes, R, Csanady, M, Casadei, B, Rudas, L (2001) Determinants of spontaneous baroreflex sensitivity in a healthy working population. Hypertension 37(3): 911-916.

Kask, A, Schioth, HB, Mutulis, F, Wikberg, JE, Rago, L (2000) Anorexigenic cocaine- and amphetamine-regulated transcript peptide intensifies fear reactions in rats. Brain Res 857(1-2): 283-285.

Kim, JH, Creekmore, E, Vezina, P (2003) Microinjection of CART peptide 55-102 into the nucleus accumbens blocks amphetamine-induced locomotion. Neuropeptides 37(6): 369-373.

Kirchheim, HR (1976) Systemic arterial baroreceptor reflexes. Physiol Rev 56(1): 100-177.

Koylu, EO, Balkan, B, Kuhar, MJ, Pogun, S (2006) Cocaine and amphetamine regulated transcript (CART) and the stress response. Peptides 27(8): 1956-1969.

Koylu, EO, Couceyro, PR, Lambert, PD, Kuhar, MJ (1998) Cocaine- and amphetamine-regulated transcript peptide immunohistochemical localization in the rat brain. J Comp Neurol 391(1): 115-132.

Koylu, EO, Couceyro, PR, Lambert, PD, Ling, NC, DeSouza, EB, Kuhar, MJ (1997) Immunohistochemical localization of novel CART peptides in rat hypothalamus, pituitary and adrenal gland. J Neuroendocrinol 9(11): 823-833.

Kristensen, P, Judge, ME, Thim, L, Ribel, U, Christjansen, KN, Wulff, BS, Clausen, JT, Jensen, PB, Madsen, OD, Vrang, N, Larsen, PJ, Hastrup, S (1998) Hypothalamic CART is a new anorectic peptide regulated by leptin. Nature 393(6680): 72-76.

Krukoff, TL, Harris, KH, Jhamandas, JH (1993) Efferent projections from the parabrachial nucleus demonstrated with the anterograde tracer Phaseolus vulgaris leucoagglutinin. Brain Res Bull 30(1-2): 163-172.

Kuhar, MJ, Adams, LD, Hunter, RG, Vechia, SD, Smith, Y (2000) CART peptides. Regul Pept 89(1-3): 1-6.

Kuhar, MJ, Yoho, LL (1999) CART peptide analysis by Western blotting. Synapse 33(3): 163-171.

Kuriyama, G, Takekoshi, S, Tojo, K, Nakai, Y, Kuhar, MJ, Osamura, RY (2004) Cocaine- and amphetamine-regulated transcript peptide in the rat anterior pituitary gland is localized in gonadotrophs and suppresses prolactin secretion. Endocrinology 145(5): 2542-2550.

La Rovere, MT, Pinna, GD, Raczak, G (2008) Baroreflex sensitivity: measurement and clinical implications. Ann Noninvasive Electrocardiol 13(2): 191-207.

Lakatos, A, Prinster, S, Vicentic, A, Hall, RA, Kuhar, MJ (2005) Cocaine- and amphetamine-regulated transcript (CART) peptide activates the extracellular signal-regulated kinase (ERK) pathway in AtT20 cells via putative G-protein coupled receptors. Neurosci Lett 384(1-2): 198-202.

Lambert, PD, Couceyro, PR, McGirr, KM, Dall Vechia, SE, Smith, Y, Kuhar, MJ (1998) CART peptides in the central control of feeding and interactions with neuropeptide Y. Synapse 29(4): 293-298.

Larsen, PJ, Seier, V, Fink-Jensen, A, Holst, JJ, Warberg, J, Vrang, N (2003) Cocaine- and amphetamine-regulated transcript is present in hypothalamic neuroendocrine neurones and is released to the hypothalamic-pituitary portal circuit. J Neuroendocrinol 15(3): 219-226.

Marie-Claire, C, Laurendeau, I, Canestrelli, C, Courtin, C, Vidaud, M, Roques, B, Noble, F (2003) Fos but not Cart (cocaine and amphetamine regulated transcript) is overexpressed by several drugs of abuse: a comparative study using real-time quantitative polymerase chain reaction in rat brain. Neurosci Lett 345(2): 77-80.

Matsumura, K, Tsuchihashi, T, Abe, I (2001) Central human cocaine- and amphetamine-regulated transcript peptide 55-102 increases arterial pressure in conscious rabbits. Hypertension 38(5): 1096-1100.

Montminy, MR, Gonzalez, GA, Yamamoto, KK (1990) Regulation of cAMP-inducible genes by CREB. Trends Neurosci 13(5): 184-188.

Murphy, KG, Abbott, CR, Mahmoudi, M, Hunter, R, Gardiner, JV, Rossi, M, Stanley, SA, Ghatei, MA, Kuhar, MJ, Bloom, SR (2000) Quantification and synthesis of cocaine- and amphetamine-regulated transcript peptide (79-102)-like immunoreactivity and mRNA in rat tissues. J Endocrinol 166(3): 659-668.

Neff, RA, Mihalevich, M, Mendelowitz, D (1998) Stimulation of NTS activates NMDA and non-NMDA receptors in rat cardiac vagal neurons in the nucleus ambiguus. Brain Res 792(2): 277-282.

Ogden, CA, Rich, ME, Schork, NJ, Paulus, MP, Geyer, MA, Lohr, JB, Kuczenski, R, Niculescu, AB (2004) Candidate genes, pathways and mechanisms for bipolar (manic-depressive) and related disorders: an expanded convergent functional genomics approach. Mol Psychiatry 9(11): 1007-1029.

Paoletti, P, Neyton, J (2007) NMDA receptor subunits: function and pharmacology. Curr Opin Pharmacol 7(1): 39-47.

Raman, IM, Tong, G, Jahr, CE (1996) Beta-adrenergic regulation of synaptic NMDA receptors by cAMP-dependent protein kinase. Neuron 16(2): 415-421.

Raptis, S, Fekete, C, Sarkar, S, Rand, WM, Emerson, CH, Nagy, GM, Lechan, RM (2004) Cocaine- and amphetamine-regulated transcript co-contained in thyrotropin-releasing hormone (TRH) neurons of the hypothalamic paraventricular nucleus modulates TRH-induced prolactin secretion. Endocrinology 145(4): 1695-1699.

Roche, KW, Tingley, WG, Huganir, RL (1994) Glutamate receptor phosphorylation and synaptic plasticity. Curr Opin Neurobiol 4(3): 383-388.

Rogge, G, Jones, D, Hubert, GW, Lin, Y, Kuhar, MJ (2008) CART peptides: regulators of body weight, reward and other functions. Nat Rev Neurosci 9(10): 747-758.

Ross, CA, Ruggiero, DA, Reis, DJ (1985) Projections from the nucleus tractus solitarii to the rostral ventrolateral medulla. J Comp Neurol 242(4): 511-534.

Salinas, A, Wilde, JD, Maldve, RE (2006) Ethanol enhancement of cocaine- and amphetamine-regulated transcript mRNA and peptide expression in the nucleus accumbens. J Neurochem 97(2): 408-415.

Scruggs, P, Dun, SL, Dun, NJ (2003) Cocaine- and amphetamine-regulated transcript peptide attenuates phenylephrine-induced bradycardia in anesthetized rats. Am J Physiol Regul Integr Comp Physiol 285(6): R1496-1503.

Scruggs, P, Lai, CC, Scruggs, JE, Dun, NJ (2005) Cocaine- and amphetamine-regulated transcript peptide potentiates spinal glutamatergic sympathoexcitation in anesthetized rats. Regul Pept 127(1-3): 79-85.

Shafton, AD, Ryan, A, Badoer, E (1998) Neurons in the hypothalamic paraventricular nucleus send collaterals to the spinal cord and to the rostral ventrolateral medulla in the rat. Brain Res 801(1-2): 239-243.

Smith, SM, Vaughan, JM, Donaldson, CJ, Fernandez, RE, Li, C, Chen, A, Vale, WW (2006) Cocaine- and amphetamine-regulated transcript is localized in pituitary lactotropes and is regulated during lactation. Endocrinology 147(3): 1213-1223.

Smith, SM, Vaughan, JM, Donaldson, CJ, Rivier, J, Li, C, Chen, A, Vale, WW (2004) Cocaine- and amphetamine-regulated transcript activates the hypothalamic-pituitary-adrenal axis through a corticotropin-releasing factor receptor-dependent mechanism. Endocrinology 145(11): 5202-5209.

Smith, Y, Kieval, J, Couceyro, PR, Kuhar, MJ (1999) CART peptide-immunoreactive neurones in the nucleus accumbens in monkeys: ultrastructural analysis, colocalization studies, and synaptic interactions with dopaminergic afferents. J Comp Neurol 407(4): 491-511.

Smith, Y, Koylu, EO, Couceyro, P, Kuhar, MJ (1997) Ultrastructural localization of CART (cocaine- and amphetamine-regulated transcript) peptides in the nucleus accumbens of monkeys. Synapse 27(1): 90-94.

Spiess, J, Villarreal, J, Vale, W (1981) Isolation and sequence analysis of a somatostatin-like polypeptide from ovine hypothalamus. Biochemistry 20(7): 1982-1988.

Stanley, SA, Small, CJ, Murphy, KG, Rayes, E, Abbott, CR, Seal, LJ, Morgan, DG, Sunter, D, Dakin, CL, Kim, MS, Hunter, R, Kuhar, M, Ghatei, MA, Bloom, SR (2001) Actions of cocaine- and amphetamine-regulated transcript (CART) peptide on regulation of appetite and hypothalamo-pituitary axes in vitro and in vivo in male rats. Brain Res 893(1-2): 186-194.

Stephenson, FA, Cousins, SL, Kenny, AV (2008) Assembly and forward trafficking of NMDA receptors (Review). Mol Membr Biol 25(4): 311-320.

Sun, MK, Guyenet, PG (1986) Effect of clonidine and gamma-aminobutyric acid on the discharges of medullo-spinal sympathoexcitatory neurons in the rat. Brain Res 368(1): 1-17.

Sunagawa, K, Sato, T, Kawada, T (2001) Integrative sympathetic baroreflex regulation of arterial pressure. Ann N Y Acad Sci 940: 314-323.

Takahashi, N, Nakagawa, M, Saikawa, T, Ooie, T, Akimitsu, T, Kaneda, K, Hara, M, Iwao, T, Yonemochi, H, Ito, M, Sakata, T (1999) Noninvasive assessment of the cardiac baroreflex: response to downward tilting and comparison with the phenylephrine method. J Am Coll Cardiol 34(1): 211-215.

Takayama, K, Miura, M (1991) Glutamate-immunoreactive neurons of the central amygdaloid nucleus projecting to the subretrofacial nucleus of SHR and WKY rats: a double-labeling study. Neurosci Lett 134(1): 62-66.

Thim, L, Kristensen, P, Larsen, PJ, Wulff, BS (1998) CART, a new anorectic peptide. Int J Biochem Cell Biol 30(12): 1281-1284.

Thim, L, Kristensen, P, Nielsen, PF, Wulff, BS, Clausen, JT (1999) Tissue-specific processing of cocaine- and amphetamine-regulated transcript peptides in the rat. Proc Natl Acad Sci U S A 96(6): 2722-2727.

Van Bockstaele, EJ, Pieribone, VA, Aston-Jones, G (1989) Diverse afferents converge on the nucleus paragigantocellularis in the rat ventrolateral medulla: retrograde and anterograde tracing studies. J Comp Neurol 290(4): 561-584.

Vicentic, A (2006) CART peptide diurnal variations in blood and brain. Peptides 27(8): 1942-1948.

Vrang, N (2006) Anatomy of hypothalamic CART neurons. Peptides 27(8): 1970-1980.

Vrang, N, Larsen, PJ, Kristensen, P (2002) Cocaine-amphetamine regulated transcript (CART) expression is not regulated by amphetamine. Neuroreport 13(9): 1215-1218.

Vrang, N, Larsen, PJ, Tang-Christensen, M, Larsen, LK, Kristensen, P (2003) Hypothalamic cocaine-amphetamine regulated transcript (CART) is regulated by glucocorticoids. Brain Res 965(1-2): 45-50.

Wang, JQ, Liu, X, Zhang, G, Parelkar, NK, Arora, A, Haines, M, Fibuch, EE, Mao, L (2006) Phosphorylation of glutamate receptors: a potential mechanism for the regulation of receptor function and psychostimulant action. J Neurosci Res 84(8): 1621-1629.

Waxman, EA, Lynch, DR (2005) N-methyl-D-aspartate receptor subtypes: multiple roles in excitotoxicity and neurological disease. Neuroscientist 11(1): 37-49.

Yermolaieva, O, Chen, J, Couceyro, PR, Hoshi, T (2001) Cocaine- and amphetamine-regulated transcript peptide modulation of voltage-gated Ca2+ signaling in hippocampal neurons. J Neurosci 21(19): 7474-7480.

Zheng, H, Patterson, LM, Berthoud, HR (2002) CART in the dorsal vagal complex: sources of immunoreactivity and effects on Fos expression and food intake. Brain Res 957(2): 298-310.