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研究生:陳君超
研究生(外文):Jyun-chao Chen
論文名稱:FHOD1對脂肪細胞的胰島素反應的影響
論文名稱(外文):The Effect of FHOD1 on Insulin Response in Adipocytes
指導教授:葉日弌葉日弌引用關係
指導教授(外文):Ji-hi Yeh
學位類別:碩士
校院名稱:慈濟大學
系所名稱:分子生物暨人類遺傳學研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
畢業學年度:97
語文別:中文
論文頁數:32
中文關鍵詞:胰島素脂肪細胞
外文關鍵詞:FHOD1insulinGlut4
相關次數:
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  • 下載下載:1
  • 收藏至我的研究室書目清單書目收藏:0
肥胖以及第二型糖尿病已成為現代普遍的代謝性疾病。第二型糖尿病
因胰島素阻抗現象而造成醣類代謝異常。脂肪組織過多與全身性的胰島素
阻抗現象關係密切,同時,不同部位的脂肪組織對於全身性代謝的調控也
有所不同。調控脂肪細胞分化及其接受胰島素刺激後反應因子的研究受到
廣泛的注意。而Glut4(SLC2A4) (Glucose transporter 4)在脂肪細胞受
胰島素刺激後,扮演增加葡萄糖運送的重要角色。過去我們實驗室利用
yeast two-hybrid system,發現Glut4 與Fhod1(Formin homology-2
domain-containing protein 1)之間有交互作用,於是我們建構了分別帶有
Fhod1 胺基酸序列1-421、1-1010、469-1165 以及完整Fhod1(1-1165)的
lentivirus vector,感染NIH/3T3 纖維母細胞後挑選穩定表達的細胞株。發
現1-421 這株細胞分化成脂肪細胞後的葡萄糖攝取實驗中的basal level 有
增加的現象。利用表達針對Fhod1 的short hairpin RNA 之lentivirus 感染
3T3-L1 脂肪先驅細胞,並不會影響分化成脂肪細胞。而在葡萄糖攝取實驗
中,發現了在胰島素刺激後,葡萄糖運輸速率恢復至基礎狀態的速度較對
照組來的慢。根據以上的實驗,我們推測Fhod1 對Glut4 自細胞膜表面回
收至細胞質內的速度有影響。
總目錄
誌謝I
中文摘要II
目錄III
序論1
材料與方法8
結果19
討論22
參考文獻23
圖25
附圖32
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19. Schulte A, Stolp B, Schonichen A, Pylypenko O, Rak A, Fackler OT, Geyer M: The human formin FHOD1 contains a bipartite structure of FH3 and GTPase-binding domains required for activation. Structure 16:1313-1323, 2008
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