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研究生:林芳因
研究生(外文):Fang-Yin Lin
論文名稱(外文):IL-1 may up-regulate the expression of IL-6 and ColIA1 mRNAs in the cells of dihydropyridine induced gingival
指導教授:呂炫堃呂炫堃引用關係
指導教授(外文):Hsein-Kun Lu
學位類別:碩士
校院名稱:臺北醫學大學
系所名稱:牙醫學系碩博士班
學門:醫藥衛生學門
學類:牙醫學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:58
中文關鍵詞:dihydropyridine induced gingival overgrowth(DIGO)IL-1βAktIL-6collagen-1real-time PCRwestern blot
外文關鍵詞:dihydropyridine induced gingival overgrowth(DIGO)IL-1βAktIL-6collagen-1real-time PCRwestern blot
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Nifedipine is a well known calcium channel blockers for treatment of hypertension and angina. The side effect as nifedipine-induced gingival overgrowth (NIGO) can be frequently found in clinic. NIGO cells, as stimulated by IL-1β, may elicit a stronger IL-6 expression and collagen production. Moreover, Akt phosphorylation was correlated to collagen overproduction. We propose a hypothesis that PI3K/Akt/NFκB pathway is one of the possible mechanisms which may be related to the over expression of IL-6 and overproduction of extra-cellular collagen in dihydropyridine induced gingival overgrowth (DIGO) cells. In this study, we will use western blot technique to detect the expression of Akt. Moreover, real-time PCR is utilized to explore the expression of IL-6 and collagen-I mRNA. Sircol dye assay was used to detect total collagen concentration. Finally, we will interpret the correlation of p-Akt/Akt, IL-6 and collagen by non-parametric Spearman correlation coefficient. Result revealed that by stimulating with IL-1β, the value of p-Akt/Akt ratio, the expression of IL-6 and collagen-I mRNA, and the concentration of IL-6 and collagen in supernatant were all increased. In addition to the expression of IL-6 mRNA, the other three factor described above were higher in DIGO group then in healthy group stimulated by IL-1β. Spearman correlation coefficient shows high relationship between the value of p-Akt/Akt, the expression of IL-6 and collagen-I mRNA, and the concentration of IL-6 in supernatant. The data mentioned above approve that Akt is in connection with gingival overgrowth.
中文摘要-------------------------------------------------1
英文摘要-------------------------------------------------2

第一章 緒論
第一節、 研究動機-------------------------------------4
第二節、 IL-1β在DIGO細胞的發炎反應中所扮演的角色-----5
第三節、 確認阻斷AR和IL-6後,nifedipine和IL-1β
的刺激對細胞發炎反應的影響-------------------6
第四節、 研究PI3K/Akt pathway在DIGO細胞中是否會
調控IL-1β到IL-6的路徑----------------------7
第二章 文獻回顧
第一節、 藥物引起牙齦增生之歷史--------------------9
第二節、 IL-6--------------------------------------10
第三節、 PI3 kinase/Akt------------------------------12
第三章、研究材料與方法
第一節、 實驗材料----------------------------------14
第二節、 實驗儀器----------------------------------16
第三節、 臨床病人選擇----------------------------17
第四節、 細胞培養--------------------------------18
第五節、 Western blot------------------------------19
第六節、 Real-time PCR----------------------------22
第七節、 ELISA----------------------------------25
第八節、 培養液上清液中Collagen之量化------------26
第九節、 統計方法--------------------------------27
第四章、實驗結果
第一節、 Akt的protein表現--------------------------29
第二節、 IL-6的mRNA表現-------------------------30
第三節、 IL-6的protein表現--------------------------31
第四節、 Collagen-1的mRNA表現--------------------32
第五節、 Collagen的protein表現----------------------33
第六節、 各個項目的相關性--------------------------34
第五章、討論
第一節、 牙齦增生指數------------------------------36
第二節、 DIGO細胞和健康細胞的差異----------------39
第三節、 牙齦發炎和DIGO的關係--------------------41
第四節、 Akt在牙齦發炎中所扮演的角色--------------42
第六章、結論------------------------------------------44
參考文獻----------------------------------------------45
表----------------------------------------------------49
圖----------------------------------------------------52
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