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研究生:高紹慈
研究生(外文):Shao-tzu Kao
論文名稱:台灣具有藥用潛力的無囊蓋盤菌之生長及其抗生實驗研究
論文名稱(外文):Priliminary studies on growth rate and antimicrobioactivity of eight discomycetes from Taiwan.
指導教授:吳美麗吳美麗引用關係
指導教授(外文):Mei-Lee Wu
學位類別:碩士
校院名稱:臺北市立教育大學
系所名稱:自然科學系碩士班
學門:工程學門
學類:材料工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:77
中文關鍵詞:盤菌生長拮抗抗生
相關次數:
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子囊菌的盤菌形體雖小,但根據文獻報導,具藥用潛力。台灣的盤菌資源相當豐富,為瞭解本土盤菌菌種的最適培養條件以及藥用開發潛力,本研究選出吳美麗寄存於食品工業研究所的八個菌株 :軟盤菌屬分類種1 (Mollisia sp.1) ,圓盤菌屬分類種(Orbilia tax. sp,), 異毛釘菌 (Lachnum abnorme), 白毛毛釘菌(Lachnum albidulum), 類核毛釘菌(Lachnum cf. sclerotii), 巴西毛釘菌(Lachnum brasiliense), 紅硬雙頭孢菌 (Dicephalospora rufocornea), 黃山蘭斯盤菌(Lanzia huangshanica), 探討這八株菌在三種不同培養基,包括:PDA, CMA, 以及添加天然基質的PN,以及五種不同溫度(15℃, 20℃, 23℃,25℃以及30℃)下培養之生長速率,找出適合菌絲體生長的培養基與溫度;再以這些盤菌最適生長條件的結果,進行八株盤菌對鬱金香褐色斑點菌(Botrytis gladiolorum)、芋病菌(Phytophthora colocasiae)、番木瓜疫霉菌(Phytophthora nicotianae)、白絹病菌(Sclerotium rolfsii)、立枯絲核菌(Rhizoctonia solani)等五株植物病原菌的對峙培養實驗藉以篩選其生物防治潛力,並同時進行對枯草桿菌(Bacillus subtilis),金黃色葡萄球菌(Staphylococcus aureus), 大腸桿菌(Escherichia coli), 綠膿桿菌(Pseudomonas aeruginosa)等四株細菌的抗生實驗;抗生實驗乃於23℃下PDB 培養液中震盪培養盤菌兩個禮拜後,取其濾液進行濾紙擴散試驗,實驗結果顯示:八個菌株較適合的溫度和培養基依次為:圓盤菌屬分類種於23℃的CMA上的生長速率最快;白毛毛釘菌、異毛釘菌以及類核毛釘菌於23℃的PN上生長速率最快;巴西毛釘菌於25℃的PDA上生長速率最快;紅硬雙頭孢菌以25℃的PN培養菌絲生長速率最快;軟盤菌屬分類種1 以及黃山蘭斯盤菌以20℃PN生長速率最快。拮抗實驗40 種對峙組合裡,八種盤菌除了對立枯絲核菌以及白絹病菌僅部份菌株有些許抑制現象外,對於其餘三株病原菌都有產生抑制現象;對鬱金香褐色斑點菌,軟盤菌屬分類種的抑制力最高;對芋疫病菌,異毛釘菌的抑制力最高;對番木瓜疫黴菌,白毛釘菌的抑制力最高,對於立枯絲核菌,類核毛釘菌的抑制力最高;對白絹病菌,白毛釘菌的抑制力最高。抗生實驗方面,軟盤菌對金黃色葡萄球菌有部份的抗生現象,其他盤菌菌種則無抗生現象,可能因為本抗生實驗採培養液離心後再用真空過濾,與前人研究方法不同,因此無法證明前人研究所得能對抗金黃色葡萄球菌的結果,未來可以進一步利用不震盪培養的研究,探測這些盤菌是否對本研究的四株細菌具抗生作
用。
In order to study the optimal growth condition for such fungi, eight endemic strains of discomycetes from BCRC were selected for this preliminary study. The studied strains are: Dicephalospora rufocornea, Lachnum brasiliense , Lanzia huangshanica, Mollisia sp.1, Lachnum abnorme, Lachnum albidulum, Lachnum cf. sclerotii, and Orbilia tax. sp. Those strains were respectively cultivated under 5 different temperatures including15, 20, 23, 25 and 30℃ as well as under 3 different kinds of media including PDA, CMA and modified PDA (PN); results of growth condition are applied to antagonism experiment of the same eight discomycetes strains against Botrytis gladiolorum, Phytophthora colocasiae, Phytophthora nicotianae, Sclerotium rolfsii and Rhizoctonia solani, to screen the biocontrol ability of the eight discomycetes;Staphyloccus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli were used as antibacterial test organisms. According to the results of growth rates, the suitable temperatures and media for studied strains are: 23℃&CMA for Orbilia tax. sp, 25℃&PDA for L. brasiliense, 25℃&PN for D. rufocornea, 23℃&PN for L. albidulum, L. abnorme, and L. cf. sclerotii, 20℃&PN for Mollisia sp.2 and Lanzia huangshanica. Except only six discomycetes shows antagonism against S. rolfsii and R. solani, all of eight discomycetes showed biocontrol activities against other pathologenic fungi. Among eight discomycetes used in this study, Mollisia sp. 1 has the highest antagonism activity aganist B. gladiolorum ; L. abnorme has the highest antagonism activity aganist P. colocasiae ; L. albidulum has the highest antagonism activitives aganist P. nicotianae and S. rolfsii ; L. cf. sclerotii has the highest antagonism activity aganist R. solani. One dicomycetes strains showed antibacterial activity. ,It is considerad that our the filtrate obtaining methode used differs from the research reporting the D. rufocornea has antibioactivity against S. aureus.
中文摘要..................................................................................................................................... i
英文摘要................................................................................................................................... iii
目錄........................................................................................................................................... iv
圖次........................................................................................................................................... vi
表次.........................................................................................................................................viii
第一章、緒論............................................................................................................................ 1
第二章、前人研究.................................................................................................................... 2
第一節、盤菌.................................................................................................................... 2
第二節、有關真菌的生長研究........................................................................................ 3
第三節、二次代謝物........................................................................................................ 4
第四節、拮抗.................................................................................................................... 6
第三章、材料與方法................................................................................................................ 8
第一節、實驗流程............................................................................................................ 8
第二節、實驗材料............................................................................................................ 9
一、研究盤菌菌株.................................................................................................... 9
二、拮抗試驗以及抗生實驗菌株............................................................................ 9
三、培養基與試藥.................................................................................................. 10
第三節、實驗方法.......................................................................................................... 12
一、菌種活化.......................................................................................................... 12
二、培養基製作...................................................................................................... 12
三、最適生長條件.................................................................................................. 12
四、盤菌對植物病原菌的拮抗實驗...................................................................... 13
五、盤菌抑制細菌試驗.......................................................................................... 15
第四章、結果.......................................................................................................................... 16
v
第一節、最適生長條件.................................................................................................. 16
一、圓盤菌科.......................................................................................................... 16
二、皮盤菌科.......................................................................................................... 19
三、核盤菌科.......................................................................................................... 22
四、晶杯菌科.......................................................................................................... 28
第二節、盤菌對植物病原菌的拮抗實驗...................................................................... 40
一、圓盤菌科.......................................................................................................... 40
二、皮盤菌科.......................................................................................................... 43
三、核盤菌科.......................................................................................................... 46
四、晶杯菌科.......................................................................................................... 52
第三節、盤菌抑制細菌試驗.......................................................................................... 64
一、圓盤菌科.......................................................................................................... 64
二、皮盤菌科.......................................................................................................... 65
三、核盤菌科.......................................................................................................... 66
四、晶杯菌科.......................................................................................................... 68
第五章、結論與討論.............................................................................................................. 72
第一節、最適生長條件生長.......................................................................................... 72
第二節、盤菌對植物病原菌的拮抗.............................................................................. 72
第三節、盤菌抑制細菌試驗.......................................................................................... 73
參考文獻.................................................................................................................................. 74
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