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研究生:張耀中
研究生(外文):Yao-Chung Chang
論文名稱:葡萄糖胺衍生物萃取與生醫材料改質應用
論文名稱(外文):Extraction of glucosamine derivatives and the applications on themodification of biomedical materials
指導教授:王鐘毅
指導教授(外文):Chung-Yih Wang
學位類別:碩士
校院名稱:大同大學
系所名稱:生物工程學系(所)
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2008
畢業學年度:97
語文別:中文
論文頁數:80
中文關鍵詞:幾丁聚醣表面改質
外文關鍵詞:chitosanmodification
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本篇目的是利用動物副產物供分離葡萄糖胺類物質,將價值較低的副產物萃取
使其成為有高經濟價值的物質。主要分為三個部分,第一部分為從雞冠萃取出玻尿
酸,目前連續萃取所得的產率分別為:第一次萃取0.50% ,第二次萃取0.63%,第
三次萃取0.13% ,玻尿酸純度則以HPLC 測試。第二部分為利用蝦殼粉萃取出幾丁
質及幾丁聚糖,結果利用100g 的蝦殼粉可獲得6.91g 的幾丁質,產率為6.91%。第
三部分則是利用猪耳朵、猪氣管來萃取軟骨素,其產率分別為7.15%與0.70%。
萃取完之後,接下來利用所得到的產物進行改質實驗,期望能在盡量不改變原
本材料性質的前提下,另外再附加上其他不同的性質,像是增加抗菌性、提昇細胞
生長或是促進凝血等等優點。由於直接以葡萄糖胺(含幾丁質、幾丁聚醣)作為手術
縫線,常因為縫線機械強度不足與耐酸性不佳的問題,在臨床上並未大量使用。我
們將生物可分解縫線經由電漿表面改質處理,浸泡於幾丁聚醣溶液並以UV 光照
射,使幾丁聚醣接枝上縫線表面,最後再測試縫線是否具有抗菌能力,從實驗的結
果得知,改質接枝後的幾丁聚醣確實可增加縫線本身的抗菌效果。另外我們也試著
將所萃取得到的軟骨素接枝至生物高分子孔洞薄膜上(聚羥基丁酯戊酯;PHBV),並
與未接枝薄膜、接枝明膠的薄膜進行比較,結果軟骨素薄膜所培養的細胞數明顯超
越其他兩組,證實軟骨素接枝於高分子薄膜表面可以提高細胞貼附的效率。
This thesis developed the extraction methods for extracting glucosamine from
animal by products. Hyaluronic acid was extracted from the comb of roosters. The goal is
to enhance production rate as well as the purity of hyaluronic acid. The production rate of
hyaluronic acid was 0.50% for the first extraction, 0.63% for the second extraction, and
0.13% for the third extraction. HPLC was used to determine the purity of the hyaluronic
acid. Chitin and chitosan from shrimp shell was another source of glucosamine. The
production yield was 6.91% when extracted with alkaline solution. Chondroitin from pig
ears and pig trachea was obtained with acetic acid extraction process. The production
yield from pig ears is 7.15% while the production yield from pig trachea is .07%.
The glucosamines obtained from animal were used in modification experiments. We
used the Plasma treatment method to graft glucosamine onto different materials, including
pHBV membrane and sutures. The cell proliferation and adherence of pHBV
(poly(3-hydroxybutyrate-co-3-hydroxyvalerate))membrane also can be further
improved by plasma treatment. Biodegradable sutures, Polyglactin, grafted with chitosan
were tested to determine it’s properties. The results indicate that modified sutures has
better antiseptic property than unmodified sutures.
目錄
謝誌ii
中文摘要iii
英文摘要iv
目錄v
圖目錄viii
表目錄x
第一章 前言1
1.1 研究背景1
1.2 玻尿酸簡介2
1.2.1 玻尿酸之結構與來源2
1.2.2 玻尿酸之特性6
1.2.3 玻尿酸之製備9
1.2.4 玻尿酸之應用11
1.3 幾丁質與幾丁聚醣簡介15
1.3.1 幾丁質與幾丁聚醣之結構與來源15
1.3.2 幾丁質與幾丁聚醣之特性17
1.3.3 幾丁質與幾丁聚醣之製備19
1.3.4 雞丁質與幾丁聚醣之應用24
1.4 軟骨素簡介28
1.4.1 軟骨素之結構與來源28
1.4.2 軟骨素之特性29
1.4.3 軟骨素之製備31
1.4.4 軟骨素之應用31
第二章 材料與方法33
2.1 萃取33
2.2 高效液相層析(High Performance Liquid Chromatography)36
2.3 傅立葉轉換紅外線光譜分析(FT-IR Analysis)38
2.4 氧氣電漿反應39
2.5 紫外光表面接枝39
2.6 拉伸試驗41
2.7 抗菌實驗41
2.8 血小板再鈣化時間測定42
2.9 紅血球溶解分析測定43
2.10 具孔洞高分子薄膜製作44
2.11 接枝反應45
2.12 細胞相容性實驗45
第三章 結果與討論47
3.1 萃取結果47
3.2 高壓液相層析(High Performance Liquid Chromatography)48
3.3 傅立葉轉換紅外線光譜分析(FT-IR Analysis)56
3.4 接枝結果57
3.5 拉伸試驗57
3.6 抗菌實驗58
3.7 血小板再鈣化時間測定59
3.8 紅血球溶解分析測定60
3.9 細胞相容性61
第四章 結論62
參考文獻65
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