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研究生:江偉碩
研究生(外文):Wei-Shuo Chiang
論文名稱:探討DTestican於果蠅翅膀與複眼中的角色
論文名稱(外文):The Role of DTestican in Drosophila Wing and Eye
指導教授:孫以瀚孫以瀚引用關係
指導教授(外文):Y. Henry Sun
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:生命科學暨基因體科學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:英文
論文頁數:44
中文關鍵詞:果蠅翅脈眼睛
外文關鍵詞:DTesticanwing veineye scarDrosophila
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人類 Testican-2 是一個帶有 heparan sulphate 的蛋白多醣,包含一個主體蛋白以及被 heparan sulphate 修飾的聚合多胺醣側鏈。研究指出 heparan sulphate 蛋白多醣在 morphogen 的運輸及其下游信號傳遞鏈扮演重要角色。在異位表達的篩選中,我們實驗室發現於果蠅全身性表達人類 testican-2 基因以及果蠅的同源基因DTestican 時,會有額外的翅脈產生。另外,在果蠅複眼同時表達 DTestican 和 unpaired(upd)基因會造成複眼組織損傷。Upd 是一種可以分泌到細胞外的蛋白並且透過活化 Jak/STAT 信號傳遞鏈進而控制細胞分裂。我推測Dtestican 蛋白影響細胞外的訊息傳遞分子或其下游信號傳遞鏈,是造成上述表型的原因。本論文的結果顯示:(1)在幼蟲的翅碟,Dtestican 蛋白不影響 Dpp 和 Hh 信號傳遞鏈。(2)Dtestican 蛋白影響翅脈生成的時間點在期。(3)Dtestican 和 Upd 蛋白並非影響過度的細胞分裂而造成複眼損傷。(4)複眼損傷過程和細胞凋亡以及STAT92E 蛋白有關。
Human Testican-2 is known as a heparan sulphate proteoglycan (HSPG) which contains core protein and heparan sulphate modified glycosaminoglycan side chains.
HSPGs are reported to play important roles in morphogen transport and signaling transduction. In an ectopic screening, ubiquitous expression of human testican-2 and its fly homolog DTestican caused extra wing-vein formation. In another screening, co-expression of DTestican and unpaired (upd) in the differentiating retinal cells caused eye scar. Upd is a secreted glycoprotein which controls cellular proliferation through JAK/STAT signal pathway. Here, I propose the above phenotypes induced by ectopic expression of DTestican may result in influencing the extracellular signaling molecules or their signal transductions. In this thesis, the results showed that (1) Dpp and Hh signaling are not affected by DTestican in larval wing disc. (2) DTestican regulates the venation in pupal stage. (3) DTestican and Upd cause eye scar not through cell overproliferation. (4) The eye scar is apoptosis and STAT92E dependent.
中文摘要........................................................................................................................2

Abstract ..........................................................................................................................3

Introduction....................................................................................................................4

Results..........................................................................................................................12

Discussion ....................................................................................................................18

Materials and Methods.................................................................................................21

Tables ...........................................................................................................................23

Figures..........................................................................................................................25

References....................................................................................................................38
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