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研究生(外文):Ting-Jui Tu
論文名稱(外文):The Role of seven-up in Drosophila Retinal Basal Glia Development
指導教授(外文):Y. Henry Sun
外文關鍵詞:seven-upRetinal Basal GliaDrosophiladevelopment
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神經膠細胞(glial cell)是神經系統的組成份子之一,對神經系統的發育及運作有多項重要功能,如提供神經細胞養分、維持神經細胞胞外離子平衡、協調神經細胞遷移、調控突觸投射、軸突成束及解束。視網膜基底神經膠細胞(retinal basal glia, RBG)是果蠅幼蟲眼碟中的一群神經膠細胞,於兩齡時期出現在視柄,當三齡幼蟲光感受體(photoreceptor, R cell)開始分化時始遷移入眼碟,並成為光感受體軸突投射的指引。過去的研究將視網膜基底神經膠細胞分為表面神經膠(surface glia, SG)、包裹神經膠(wrapping glia, WG)、及毯神經膠(carpet glia)三種。依照序貫分化模型(sequential differentiation model),表面神經膠細胞從視柄進入眼碟基部後順著毯神經膠胞質延伸(cytoplasmic extension)向前遷移,及至延伸前緣接觸光感受體後開始分化為包裹神經膠細胞。seven-up (svp)基因乃一於多物種間高度保守的孤生類固醇受體(orphan steroid receptor),在多種果蠅細胞的發育過程中扮演重要功能。過去的研究中我們發現svp基因表現於視網膜基底神經膠細胞,暗示svp可能參與視網膜基底神經膠的發育。本篇研究中,我們首先發現Cut蛋白特定表現於包裹神經膠及分化中的神經膠細胞,可做為區分不同神經膠細胞種類的標記。在關於svp基因的研究中,我們觀察到svp突變的神經膠細胞株(mutant clone)傾向集中在前緣,細胞多呈圓型並表現Cut蛋白。此外,抑制Svp蛋白在神經膠細胞中表現會促使視網膜基底神經膠細胞及包裹神經膠細胞數量增加;而抑制Svp在包裹神經膠細胞中表現則會影響其包裹功能。綜合以上所述,本篇研究證明svp基因確實參與了視網膜基底神經膠細胞的發育並在神經膠細胞分化過程扮演重要角色。
Glial cells play various roles in nervous system development and function in both vertebrates and invertebrates. They nourish and maintain homeostasis of neurons, guide neuronal migration and axon projection, and regulate axon fasciculation and defasciculation. Retinal basal glia (RBG) is a subset of glial cell that originates in the optic stalk in second instar of Drosophila. RBG cells start to migrate into the eye disc as photoreceptor cells (R cell) begin to differentiate. Reciprocally, the presence of RBG cells in eye discs is essential for R cell axons to enter the optic stalk. Previous work has categorized RBG cells into three different types, carpet glia, surface glia, and wrapping glia. According to the sequential differentiation model proposed by Silies et al., surface glia located at the basal side migrate forward along the carpet glia. Once they reach the margin of carpet glia and contact the neuron, migratory glia start to differentiate into wrapping glia and fasciculate R cell axons. seven-up (svp) is an orphan receptor of the steroid receptor superfamily and is highly conserved with its human homolog COUP-TFs. Recent work has shown that svp plays important roles in the development of various cell types. Previously, our lab identified svp is expressed in RBG cells, suggested that svp may participate in RBG cells development. In this study, I provided evidences suggesting that cut is specifically expressed in WG and differentiating glia in the eye disc, and can serve as a WG maker. Then, I showed that svp mutant clones tend to locate on the frontier among all RBG cells, they usually rounded in shape and express Cut protein. Pan-glial knockdown Svp increases RBG cell and wrapping glia cell numbers; knockdown Svp in wrapping glia affects glial wrapping. To conclude, these results indicating that svp plays important roles in the development of RBG cells and is essential for glial differentiation.
中文摘要 1
Abstract 2
Introduction 3
Results 9
Discussion 18
Materials and Methods 25
Reference 28
Figures 32
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