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研究生:吳振棋
研究生(外文):Cheng-Chi Wu
論文名稱:探討超快雷射脈衝對細胞生長之影響與對多層奈米碳管加工的應用
論文名稱(外文):The effects and applications of ultrafast laser pulses on the growth of cell culture and the processing of multiwall carbon nanotubes
指導教授:高甫仁
指導教授(外文):Fu-Jen Kao
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:生醫光電工程研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:79
中文關鍵詞:脈衝雷射多層包覆之奈米碳管脈衝寬度雷射剝削奈秒飛秒震盪波氣穴機械應力活體細胞
外文關鍵詞:Pulse LaserMulti-Wall Carbon NanotubesPulse DurationLaser AblationNanosecondFemtosecondShockwaveCavitationMechanical StressLiving Cell
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本研究乃採用超快脈衝雷射之雷射剝削效應於材料表面處理之應用探討多層奈米碳管及生物材料之特性。本研究之實驗有二,首先藉由奈、飛秒雷射之不同脈衝寬度之雷射剝削效應探討脈衝雷射於多層奈米碳管之材料表面之變化,其次利用飛秒雷射於溶液中產生機械應力影響活體細胞,探討雷射產生機械應力影響活體細胞之刺激生長或抑制生長與否。

首先藉由脈衝雷射對於多層包覆之奈米碳管之研究,利用雷射之不同脈衝寬度造成雷射剝削效應,探討雷射於材料之處理於奈米碳管之應用,其實驗結果我們發現利用奈秒雷射及飛秒雷射之雷射剝削效應在奈米碳管之應用,其內部表面結構會因不同脈衝寬度,而造成不同之表面形變,藉由本實驗可驗證其脈衝雷射之不同脈衝寬度造成材料表面之形變。

其次利用飛秒雷射於溶液中產生震盪波及氣穴現象造成機械應力之機制,藉由雷射造成機械應力各別影響不同之生物活體細胞體外之周圍環境。脈衝雷射造成機械應力於不破壞活體細胞為前提之下,受到機械應力之影響活體細胞之周圍環境刺激活體細胞之生長或抑制與否,因此藉由生物活體細胞體外之機械應力刺激影響活體細胞體內之生長機制,探討生物細胞是否能憑藉外在機械應力刺激細胞之成長機制。
The reported study in this thesis is twofold. The research work is aimed to investigate the effect of ultrafast laser on multiwall carbon nanotubes through ablation and on cell culture through laser induced shockwave.

In the ablation based material processing, nano- and femtosecond laser pulses are focused to engrave fine structures on multi-layer carbon nanotube coated surface. The effects of ablation are compared. We found that both nano- and femtosecond laser pulses produced approximately the same spatial resolution or definition. Femtosecond laser pulses, however, is able to generate highly clean-cut features without much residue. For comparison, more residue were found with nanosecond laser pulses

Laser-induced shockwave and cavitation is also used to generate mechanical stress on living cultured cells. We found that the laser-induced mechanical stress strongly affected the cultured cells by severely limiting or stimulating the growth, depending on the types of the cells. The growth of P19CL6 (Embryonal carcinoma) and C2C12 (muscle) cell cultures is greatly stimulated, while the growth of PC12 (Pheochromocytoma) and HeLa (cervix) is severely inhibited. Very interesting interpretation is thus implied by the above results and forms the direction of future investigation.
第一章. 導論 ……………………………………………………… 01
1-1. 前言 ………………………………………………………… 01
1-2. 實驗方法 …………………………………………………… 03
1-3. 實驗目的 …………………………………………………… 04

第二章. 實驗原理 ………………………………………………… 08
2-1. 雷射與物質之交互作用 …………………………………… 08
2-2. 雷射與組織之交互作用 …………………………………… 11

第三章. 實驗材料 ………………………………………………… 16
3-1. 多層奈米碳管 ……………………………………………… 16
3-2. 生物活體細胞 ……………………………………………… 19
3-2-1. P19CL6細胞 ……………………………………………… 19
3-2-2. PC12細胞 ………………………………………………… 21
3-2-3. HeLa細胞 ………………………………………………… 23
3-2-4. C2C12細胞 ……………………………………………… . 23

第四章. 實驗架設 ………………………………………………… 27
4-1. 不同脈衝雷射對奈米碳管之影響 ………………………… 27
4-1-1. 奈秒雷射系統 …………………………………………… 28
4-1-2. 鎖模飛秒雷射系統 ……………………………………… 30
4-2. 飛秒雷射產生應力對活體細胞之影響 …………………… 32

第五章. 實驗結果及討論 ………………………………………… 37
5-1. 不同脈衝雷射對奈米碳管之影響 ………………………… 37
5-1-1. 奈秒雷射對奈米碳管之影響 …………………………… 38
5-1-2. 飛秒雷射對奈米碳管之影響 …………………………… 45
5-2. 飛秒雷射產生應力對活體細胞之影響 …………………… 54
5-2-1. 飛秒雷射產生應力對P19CL6細胞之影響 ……………… 54
5-2-2. 飛秒雷射產生應力對PC12細胞之影響 ………………… 56
5-2-3. 飛秒雷射產生應力對HeLa細胞之影響 ………………… 58
5-2-4. 飛秒雷射產生應力對C2C12細胞之影響 ………………… 60

第六章. 結論 ……………………………………………………… 68
6-1. 不同脈衝雷射對奈米碳管之影響 ………………………… 68
6-2. 飛秒雷射產生應力對活體細胞之影響 …………………… 69

第七章. 未來展望 ………………………………………………… 71

附錄 ………………………………………………………………… 73
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