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研究生:林智庸
研究生(外文):Chih-Yung Lin
論文名稱:運動和乳酸對雄鼠分泌醛固酮之效應
論文名稱(外文):Effects of Exercise and Lactate on the Secretion of Aldosterone in Male Rats
指導教授:王錫崗王錫崗引用關係汪貴珍汪貴珍引用關係
指導教授(外文):Paulus S. WangGuei-Jane Wang
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:生理學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:英文
論文頁數:42
中文關鍵詞:運動醛固酮乳酸
外文關鍵詞:exercisealdosteronelactate
相關次數:
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醛固酮(aldosterone)是體內主要的礦物皮質荷爾蒙,由腎上腺皮質分泌。過去研究顯示運動會使醛固酮分泌增加,然而其中的機制仍不明瞭。本研究利用游泳探討運動前後醛固酮分泌之變化,將大鼠分為四組,分別是浸水組(water immersion)、游泳組(swim)、乳酸灌流組(lactate infusion)、丙酮酸灌流組(pyruvate infusion)。前一天先於右頸靜脈埋置軟管,隔日讓大鼠分別游泳或灌流十分鐘,並於實驗開始0,10,15,30,60,120分鐘採血進行各項內分泌檢測。離體實驗中將大鼠腎上腺皮質球狀帶細胞(zona glomerulosa cells)分離出後,直接給予乳酸或血管張力素II(angiotensin II)進行離體培養60分鐘後,收集上清液和細胞進行放射免疫測定醛固酮濃度和西方墨點法測定StAR protein與P450scc蛋白表現。結果發現運動後醛固酮上升,乳酸灌流組也有相似的效應。而離體細胞實驗發現直接給予乳酸並未刺激醛固酮分泌,然而同時給予運動時濃度(10 mM)乳酸與血管張力素II時可以刺激醛固酮的分泌並增加StAR protein的表現。因此推論乳酸可以促進運動中醛固酮的分泌,並可能透過腎素-血管張力素-醛固酮系統中增加血管張力素II所致。
Aldosterone is a steroid hormone produced by the zona glomerulosa (ZG) cells of the adrenal cortex in the adrenal gland. Previous studies have demonstrated that exercise is one of the stresses known to increase the aldosterone level. Both potassium and angiotensin II levels were correlated with aldosterone production during exercise, but the mechanism is still unclear. In an in vivo study, male rats were catheterized via right jugular vein (RJV), and divided into four groups including immersion, swim, lactate infusion and pyruvate infusion (13 mg/kg/min) groups. Each group treated for 10 min. Blood samples were collected at 0, 10, 15, 30, 60 and 120 min from RJV after administration. In an in vitro study, ZG cells from male rats were challenged by lactate (1-10 mM) with or without angiotensin II (Ang II, 10-8 M) for 60 min. The levels of aldosterone in plasma and medium were measured by radioimmunoassay. Cell lysate were measured by immunoblotting. After exercise and lactate infusion, plasma levels of aldosterone and lactate were significantly higher than those in the control group. Administration of lactate and angiotensin II significantly increased aldosterone production and enhanced StAR protein expression in ZG cells. These results demonstrated that short-time exercise led to the increase of aldosterone secretion, which is associated with lactate action on ZG cells and might to depend on the activity of renin-angiotensin system.
Chinese abstract ............................................ 1
English abstract............................................. 2
I. Introduction.................................3
1. Lactic acid
2. Exercise and lactate
3. Exercise and endocrinology
4. Aldosterone
II. Materials and Methods........................10
1. Experimental animals
2. In vivo experiments
3. In vitro experiments
4. RIA of aldosterone
5. Statistical Analysis
III. Results......................................16
1. Effect of swimming on plasma glucose, lactate, Na+, K+, osmolality, aldosterone and angiotensin II
2. Effect of lactate infusion on plasma glucose, lactate, Na+, K+, osmolality, aldosterone and angiotensin II
3. Effects of lactate on basal, Ang II, ACTH and KCl-related aldosterone production in vitro
4. Effect of lactate on expressions of StAR protein and P450scc
IV. Discussion...................................18
V. References...................................22
VI. Figures......................................28
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