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研究生:李俊霖
研究生(外文):Jyun-Lin Lee
論文名稱:人類絨毛膜性腺激素對去卵巢鼠胃腸活動之影響
論文名稱(外文):Effects of Human Chorionic Gonadotropin on Gastrointestinal Motility in Ovariectomized Rats
指導教授:董明倫
指導教授(外文):Ming-Luen Doong
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:生理學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:46
中文關鍵詞:人類絨毛膜性腺激素胃排空膽囊收縮素
外文關鍵詞:hCGgastric emptyingCCK
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妊娠劇吐症是指在早期懷孕期間孕婦有噁心及嘔吐等症狀。此時通常會伴隨著孕婦體重減輕、食慾變差以及脫水等現象。然而,到目前為止真正引起妊娠劇吐症發生的原因,仍然不清楚。目前一般的推測為此症狀的發生可能和懷孕期間體內荷爾蒙的改變有關。研究指出,在懷孕初期,孕婦體內的人類絨毛膜性腺激素會持續的增加。直到四個月後,其濃度才會慢慢的下降。人類絨毛膜性腺激素對於胃腸道活動的影響,過去很少被研究,到目前仍是呈現未知的情況。此外,已知膽囊收縮素對於胃腸道活動的影響扮演著很重要的角色。但是其和人類絨毛膜性腺激素之間的關係,較少被討論到。因此本研究旨在探討人類絨毛膜性腺激素對於胃腸道活動之影響,以及其和膽囊收縮素之間的關係。利用八週大雌鼠,進行卵巢切除手術後,穩定二週。去卵巢鼠由腹腔分別注射不同劑量的人類絨毛膜性腺激素(0, 50, 100, 200 IU),持續注射三天後,檢測其胃排空及小腸通過率,觀察人類絨毛膜性腺激素對於胃腸運動之效應。同時收集血漿,以酵素免疫測定法檢測血漿中膽囊收縮素濃度之變化。另由中樞及週邊給予膽囊收縮素受體拮抗劑於前處理人類絨毛膜性腺激素之去卵巢鼠,觀察膽囊收縮素是否參與人類絨毛膜性腺激素對胃腸活動之效應。本研究結果顯示(1)雌鼠卵巢切除後其胃排空增加,且子宮重量相較於對照組動情間期之雌鼠有明顯減低的現象。(2) 人類絨毛膜性腺激素抑制了去卵巢鼠胃排空作用,並呈劑量-反應效應。(3) 人類絨毛膜性腺激素可增加血漿中膽囊收縮素之濃度,並呈劑量-反應效應。(4) 去卵巢鼠經由週邊給與CCK1受體拮抗劑lorglumide後,可顯著的恢復因人類絨毛膜性腺激素所導致的胃排空抑制作用。(5)去卵巢鼠經由中樞給與CCK1受體拮抗劑lorglumide後,對於因人類絨毛膜性腺激素所導致胃排空之抑制作用則無影響。由以上結果得知,人類絨毛膜性腺激素具有抑制胃排空之效應,而此效應,可能是透過增加血漿中膽囊收縮素濃度後,再經由週邊CCK1受體來作用。
Vomiting concurs with high levels of human chorionic gonadotropin (hCG) and a prolongation of GI transit has been reported during pregnancy, but the mechanism is uncertain. These observations have led to speculation about the possible role of hCG on gastrointestinal (GI) function. In addition, cholecystokinin (CCK) is well known to inhibit gastric emptying. Therefore, the aims of this study were to investigate the effects of hCG on GI motility in ovariectomized (Ovx) rats, and to clarify the role of CCK in regulating the hCG effects on GI motility. GI motility was assessed in rats 15 min after intragastric instillation of a test meal containing charcoal and Na251CrO4. Gastric emptying was determined by measuring the amount of labeled chromium contained in the small intestine as a percentage of the initial amount received. Intestinal transit was assessed by calculating the geometric center of distribution of the radiolabeled marker. CCK concentrations in the plasma were measured by enzymeimmunoassay. The results showed that after intraperitoneal administration of hCG (50, 100 and 200 IU) 3 days, gastric emptying was decreased in a dose-dependence manner but intestinal transit was not affected. Plasma CCK levels were increased dose-dependently by hCG. Peripheral administration of lorglumide, a selective CCK1 receptor antagonist, effectively attenuated the hCG-induced inhibition of gastric emptying. Central administration of lorglumide did not alter the hCG -induced inhibition of gastric emptying. These results suggest that hCG inhibits gastric emptying in Ovx rats via a peripheral mechanism involving the stimulation of CCK release and the activation of CCK1 receptor.
圖錄 ………………………………………………………… I
重要名詞中英文對照表 …………………………………………… Ⅱ
中文摘要 ………………………………………………………… 1
英文摘要 ………………………………………………………… 3
第一章、文獻回顧 ……………………………………………… 4
一、概論 ……………………………………………… 5
二、人類絨毛膜性腺激素…………………………………………… 5
(一)人類絨毛膜性腺激素之分泌 ………………………… 5
(二)人類絨毛膜性腺激素之受體 ………………………… 6
(三)人類絨毛膜性腺激素之生理功能 …………………… 6
三、膽囊收縮素 …………………………………………………… 7
(一)膽囊收縮素之分泌 ……………………………… 7
(二)膽囊收縮素之受體 ……………………………… 8
(三)膽囊收縮素對胃腸系統之影響 ……………………… 8
四、目的與假說 …………………………………………………… 10

第二章、材料與方法………………………………………………… 12
一、實驗動物 …………………………………………………… 13
二、實驗設計 …………………………………………………… 13
三、主要實驗技術…………………………………………………… 15
四、藥物來源 …………………………………………………… 17
五、統計分析 …………………………………………………… 18


第三章、結果 …………………………………………… 19
一、動情間期(diestrus)雌鼠與去卵巢鼠胃腸運動比較 …… 20
二、人類絨毛膜性腺激素對去卵巢鼠胃腸運動之劑量效應 …… 20
三、人類絨毛膜性腺激素對去卵巢鼠血漿膽囊收縮素濃度
之劑量效應 …………………………………………… 21
四、週邊CCK1受體拮抗劑 lorglumide對人類絨毛膜性腺
激素處理之去卵巢鼠胃腸運動之影響 ………………… 22
五、中樞CCK1受體拮抗劑 lorglumide對人類絨毛膜性腺
激素處理之去卵巢鼠胃腸運動之影響 ………………… 22

第四章、討論 ……………………………………………………… 24
參考文獻 ………………………………………………………… 27
圖與圖誌 ………………………………………………………… 35
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