跳到主要內容

臺灣博碩士論文加值系統

(3.235.120.150) 您好!臺灣時間:2021/07/31 13:53
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

: 
twitterline
研究生:鄭雅方
研究生(外文):Ya-Fang Cheng
論文名稱:不同模式刺激大白鼠內關穴對心臟熱休克蛋白三十二表現的調控
論文名稱(外文):Modulatory Effects of Multimodality Stimulation at Neiguan (PC 6) on Myocardial Hsp32 Expression in Rats
指導教授:邱仁輝邱仁輝引用關係
指導教授(外文):Jen-Hwey Chiu
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:傳統醫藥學研究所
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:110
中文關鍵詞:針灸熱休克蛋白缺血再灌流傳統醫藥
外文關鍵詞:acupmoxaheat shock proteinischemia-reperfusiontraditional medicine
相關次數:
  • 被引用被引用:0
  • 點閱點閱:134
  • 評分評分:
  • 下載下載:7
  • 收藏至我的研究室書目清單書目收藏:0
熱休克蛋白三十二 (Hsp32),是一種具有保護心臟功能的蛋白質。本研究之目的是為了探討不同模式刺激大白鼠左側內關穴調控心臟Hsp32表現的影響,與其保護心臟缺血再灌流傷害的可能機轉。將雄性Sprague-Dawley大鼠分為六個組別:正常組、電針組(EA)、雷射針刺組(LA)、藥針組(HA)、溫灸組(LSTS)和偽實驗組(Sham)。本研究 (除HA組不需麻醉以外),其他的刺激模式均施予在適當麻醉的大白鼠左側內關穴。各模式的刺激參數如下,電針頻率為2/15 Hz的交替波、強度1-2 mA、刺激時間為20分鐘;雷射針刺使用的激光波長為660 nm、輸出功率60 mW,刺激時間為15 分鐘;藥針使用的劑量為3mg/100g BW的chloralose和urethane混合液; 溫灸採用的是波動式溫度、每次刺激時間為27分鐘且施行三次。在病理檢測上,利用免疫螢光染色法、西方點墨法以及即時偵測同步定量聚合酶鏈鎖反應來偵測心肌hsp32基因的表現;大鼠血清之生化檢測項目為CK-MB與Troponin-I,用來評估心肌損傷;在心臟缺血再灌流的實驗中,記錄心電圖波形以偵測心律不整的發生率。最後利用初級培養來探討藥物 (HA)對心臟組織的直接效應。由結果得知,在不同模式刺激下,只有在大鼠左側內關穴施予HA刺激,能夠顯著性地使心臟hsp32基因和Nrf2蛋白質表現增加且Hsp32蛋白表現具有時間效應與劑量效應。而施予LSTS刺激則可促進心臟Hsp70蛋白表現量的上升。由免疫染色可以得知,Hsp32蛋白主要表現在心肌與內皮細胞,但在升主動脈中,只表現在平滑肌細胞內。體外心臟組織初級培養實驗的結果為Nrf2蛋白的表現與HA藥物的直接作用效應有關且單一藥物Urethane即具有促進Nrf2蛋白表現的作用。此外,前置處理HA於內關穴能夠減少缺血再灌流心臟的心律不整發生率。本研究之結論為在左側內關穴施予HA刺激能夠誘導hsp32 基因與Nrf2蛋白表現增加,且能夠減少缺血再灌流心臟的心律不整發生率,為臨床缺血性心臟病的防治提供了一個新的治療策略。
Hsp32 is a stress-inducible protein with prominent cardio-protective functions. The aim of this study was to investigate the effect of multimodality stimulations at acupoint PC 6 on myocardial Hsp32 expression in rats.
Male Sprague-Dawley rat, weighing 260~320g, were divided into six groups, namely, lectroacupuncture group (EA), laser acupuncture group (LA), hydroacupuncture group (HA), local somatothermal stimulation group (LSTS), sham group (for EA, LA, LSTS) and normal group (for HA), under adequate anesthesia, multimodalities, such as EA, LA, HA and LSTS were separately applied at left acupoint Neiguan (PC 6). The parameters were 2/15 Hz, alternatively, 1-2 mA and 20min for EA; semiconductor laser, emission wavelengths of 660 nm, 60 mW and 15 min for LA; a dose of 3mg/100g body weight of chloralose and urethane mixture for HA; fluctuating temperature, 27min/per dose and three doses for LSTS treatment. The pathological examinations, myocardial Hsp32 protein and mRNA expression were detected by using immunostaining, Western blotting, and real time PCR, respectively. Serum CK-MB and Troponin-I were determined to evaluate the possibility of myocardium injury after HA treatment at PC 6 acupoint . Electrocardiography was recorded to detect the incidence of arrhythmia in ischemia-reperfusion (I/R) model. Besides, primary endocardium and myocardium cultures were used to investigate the direct effect of drug mixture , which was used for in vivo HA treatment, on cardiac Hsp32 expression.
The results showed that there was a significant increase of Hsp32 and Nrf2 expression in HA group and an increase of Hsp70 in LSTS group. Hsp32 was time-dependently and dose-dependently increased by HA treatment. However, there was no significant difference, in terms of IκB expression, in all experimental groups. Results from immunostaining showed that the Hsp32 expression was located in myocardium and endocardium in the heart, but mainly in smooth muscle layer of ascending aorta. By using primary heart cultures, Nrf2 protein expression was increased in both endocardium and myocardium. Besides, preconditioning HA at left PC 6 significantly decreased the duration of arrhythmia (VPB, VF and total account) and incidence of stony heart in subsequent I/R model. We concluded that LSTS at left PC 6 increased Hsp70 protein expression while HA at left PC 6 increased hsp32 gene expression and Nrf2 protein expression, which might provide a new strategy for treatment of ischemic heart disease.
目次
目次 I
表次及圖次 VI
中文摘要 VIII
英文摘要 X
第一章 緒論
第一節、常見心臟疾病概論 2
一、心臟疾病分類 2
二、心臟缺血再灌流傷害 3
(一) 臨床表徵 3
(二) 心臟缺血再灌流傷害之致病機轉 4
(三) 心臟缺血再灌流傷害之治療與預防-手術或藥物 6
三、Hsp32在心臟缺血再灌流傷害中扮演的角色 9
第二節、古典中醫觀念 11
一、古典文獻對於「心」的描述 11
二、中醫藥對心臟疾病的治療 14
(一) 針灸治病的原理 14
(二) 經絡參與病理過程及反應疾病證候 14
(三) 中醫藥療法簡介 15
(四) 中醫藥對心臟功能調控的近代研究 20
• 人體觀察 20
• 動物實驗 24
第三節、研究動機與目的 27
第二章 實驗材料及方法
ㄧ、設備及藥品 30
1. 儀器設備 30
2. 化學藥品及試劑 32
二、實驗動物 37
三、動物麻醉藥物與方法 37
四、針灸的參數 38
1. 穴位的選擇 38
2. 電針(EA)、雷射針刺(LA)、溫灸(LSTS)的參數與時間效應 38
3. 藥針刺激(HA)參數與操作方式 39
五、心臟組織初級培養(Primary culture)的建立 40
六、心臟缺血再灌流(I/R)動物模式的建立 41
七、心臟組織之蛋白質與基因分析 42
(一) Hsp32、核轉錄因子Nrf2蛋白含量的測定 42
1.蛋白質萃取 42
2.蛋白質定量 43
3.電泳分離 43
4.西方點墨法 44
(二) RNA定性與半定量 45
1.RNA萃取 45
2.互補DNA 製程 (Complementary DNA / cDNA ) 47
3.逆轉錄聚合酶連鎖反應(Reverse Transcription-PCR) 47
4.即時性聚合酶連鎖反應(Real time-PCR) 48
(三) 免疫螢光染色 49
(四) 生理評估項目 50
1.心電圖的紀錄 50
2.血清生化檢驗 51
3. 藥針(HA)刺激對大鼠生理狀態的影響評估 51
(五) 實驗數據和統計方法 52
第三章 結果
(一)不同模式刺激內關穴對心臟Hsp32、Hsp70與其相關蛋白表現
的影響 54
(二) LA對Hsp32蛋白表現之時間效應 54
(三) HA刺激大鼠左側內關穴對Hsp32蛋白與基因表現量的影響 55
1. HA刺激產生Hsp32蛋白的時間效應 55
2. HA刺激產生Hsp32蛋白的劑量效應 55
3. HA刺激產生Hsp32基因表現的時間效應 56
4. HA刺激產生Hsp32蛋白的溶劑 (vehicle)影響 56
5. HA刺激產生Hsp32蛋白的藥物影響 57
(四) HA刺激大鼠左側內關穴對核轉錄因子Nrf2蛋白的影響 57
1. HA刺激產生核轉錄因子Nrf2蛋白的時間效應 57
(五) Hsp32蛋白在心臟組織中的表現位置 58
(六) HA刺激對大鼠心臟及生理狀態的影響 58
1. HA刺激大鼠左側內關穴對心臟酵素CK-MB 與Troponin-I
的影響 58
2. HA刺激對大鼠生理狀態影響的評估 59
(七) 在心臟缺血再灌流 (I/R)模型下Hsp32對心臟的保護作用 59
1. 心電圖紀錄與心律不整發生率之評估 59
2. 內關穴前置HA刺激對缺血再灌流心臟Hsp32蛋白表現
影響 60
(八) HA藥物對大鼠心臟細胞的直接效應 60
第四章 討論
(一) 麻醉藥對Hsp32表現的影響 63
(二) 動物內關穴的選取 63
(三) 多樣模式參數的選擇 (EA、LA、LSTS) 64
(四) 多樣模式刺激與Hsp32、 Hsp70之相關性 67
(五) 體表多樣模式刺激之神經傳導與相對應內臟反射 69
1. 內臟-內臟反射(visceral-visceral reflex) 69
2. 體表-內臟反射(somato-visceral reflex) 70
3. 化學性刺激模式HA的直接效應 71
(六) 影響Hsp32表現的其他因子與生化路徑 72
(七) 臨床實用性 73
1. 心電圖結果討論 73
第五章 結論 75
第六章 參考文獻 77
第七章 圖表附錄 88
1. Abraham NG and Kappas A. Heme oxygenase and the cardiovascular-renal system. Free Radic Biol Med. 2005;39:1-25.
2. Akhlaghi M and Bandy B. Mechanisms of flavonoid protection against myocardial ischemia-reperfusion injury. J Mol Cell Cardiol. 2009;46:309-17.
3. Alam J and Cook JL. How many transcription factors does it take to turn on the heme oxygenase-1 gene? Am J Respir Cell Mol Biol. 2007;36:166-74.
4. Bechtold DA and Brown IR. Induction of Hsp27 and Hsp32 stress proteins and vimentin in glial cells of the rat hippocampus following hyperthermia. Neurochem Res. 2003;28:1163-73.
5. Bentley DE, Watson A, Treede RD, Barrett G, Youell PD, Kulkarni B, Jones AK. Differential effects on the laser evoked potential of selectively attending to pain localisation versus pain unpleasantness. Clin Neurophysiol. 2004;115:1846-56.
6. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
7. Cao Q, Liu J, Chen S, Han Z. Effects of electroacupuncture at neiguan on myocardial microcirculation in rabbits with acute myocardial ischemia. J Tradit Chin Med. 1998;18:134-9.
8. Chang S, Chao WL, Chiang MJ, Li SJ, Lu YT, Ma CM, Cheng HY et al. Effects of acupuncture at Neiguan (PC 6) of the pericardial meridian on blood pressure and heart rate variability. Chin J Physiol. 2008;51:167-77.
9. Chang YJ. Study of Autonomic Nervous Activities for the Night Shift Workers by Laser Acupuncture. 2007.
10. Chen HC. Effects of moxibusting Point Kuan-Yuan on cardiovascular and renal responses to histamine-induced shock. Am J Chin Med. 1987;15:77-82.
11. Chen SC, Lu SN, Lai CT, Jean JY, Hsiao CL, Hsu PT. Aqueous Acupuncture for Postoperative Pain – A Matched Controlled Trial. The Kaohsiung Journal of Medical Sciences. 1991;7:466-70.
12. Chiu JH, Lui WY, Chen YL, Hong CY. Local somatothermal stimulation inhibits the motility of sphincter of Oddi in cats, rabbits and humans through nitrergic neural release of nitric oxide. Life Sci. 1998;63:413-28.
13. Chiu JH, Tsou MT, Tung HH, Tai CH, Tsai SK, Chih CL, Lin JG et al. Preconditioned somatothermal stimulation on median nerve territory increases myocardial heat shock protein 70 and protects rat hearts against ischemia-reperfusion injury. J Thorac Cardiovasc Surg. 2003;125:678-85.
14. Chow AM and Brown IR. Induction of heat shock proteins in differentiated human and rodent neurons by celastrol. Cell Stress Chaperones. 2007;12:237-44.
15. Dulak J, Deshane J, Jozkowicz A, Agarwal A. Heme oxygenase-1 and carbon monoxide in vascular pathobiology: focus on angiogenesis. Circulation. 2008;117:231-41.
16. Ellis RJ and van der Vies SM. Molecular chaperones. Annu Rev Biochem. 1991;60:321-47.
17. Ewing JF, Raju VS, Maines MD. Induction of heart heme oxygenase-1 (HSP32) by hyperthermia: possible role in stress-mediated elevation of cyclic 3':5'-guanosine monophosphate. J Pharmacol Exp Ther. 1994;271:408-14.
18. Gao J, Fu W, Jin Z, Yu X. A preliminary study on the cardioprotection of acupuncture pretreatment in rats with ischemia and reperfusion: involvement of cardiac beta-adrenoceptors. J Physiol Sci. 2006;56:275-9.
19. Gao J, Zhang L, Wang Y, Lu B, Cui H, Fu W, Wang H et al. Antiarrhythmic effect of acupuncture pretreatment in rats subjected to simulative global ischemia and reperfusion--involvement of adenylate cyclase, protein kinase A, and L-type Ca2+ channel. J Physiol Sci. 2008;58:389-96.
20. Giannini L, Vannacci A, Fabrizi F, Uliva C, Bani D, Masini E, Mannaioni PF. Protection from cardiac injury by induction of heme oxygenase-1 and nitric oxide synthase in a focal ischaemia-reperfusion model. Cell Mol Biol (Noisy-le-grand). 2005;51:393-401.
21. Hagiwara S, Iwasaka H, Matsumoto S, Noguchi T, Yoshioka H. Association between heat stress protein 70 induction and decreased pulmonary fibrosis in an animal model of acute lung injury. Lung. 2007;185:287-93.
22. Hu CM, Chen YH, Chiang MT, Chau LY. Heme oxygenase-1 inhibits angiotensin II-induced cardiac hypertrophy in vitro and in vivo. Circulation. 2004;110:309-16.
23. Huang LP, Zhou S, Lu Z, Tian Q, Li X, Cao LJ, Yu JH et al. Bilateral effect of unilateral electroacupuncture on muscle strength. J Altern Complement Med. 2007 Jun;13:539-46.
24. Hwang BT, Kim JS, Hwang UJ. Ultrastructural studies on the Effect of Aqua-acupuncture to Liver Damage induced by Radia Aconiti. Journal of Korean Institute of Herbal Acupuncture. 1997;1:22-34.
25. Idriss NK, Blann AD and Lip GY. Hemoxygenase-1 in cardiovascular disease. J Am Coll Cardiol. 2008;52:971-8.
26. Ishihara Y, Sekine M, Nakazawa M, Shimamoto N. Suppression of myocardial ischemia-reperfusion injury by inhibitors of cytochrome P450 in rats. Eur J Pharmacol. 2009;611:64-71.
27. Jiang B, Zhang L, Li M, Wu W, Yang M, Wang J, Guo DA. Salvianolic acids prevent acute doxorubicin cardiotoxicity in mice through suppression of oxidative stress. Food Chem Toxicol. 2008;46:1510-5.
28. Jiang JK, Chiu JH, Lin JK. Local thermal stimulation relaxes hypertonic anal sphincter: evidence of somatoanal reflex. Dis Colon Rectum. 1999;42(9):1152-9.
29. Kinderlerer AR, Pombo Gregoire I, Hamdulay SS, Ali F, Steinberg R, Silva G, Ali N et al. Heme oxygenase-1 expression enhances vascular endothelial resistance to complement-mediated injury through induction of decay-accelerating factor: a role for increased bilirubin and ferritin. Blood. 2009;113:1598-607.
30. Lakkisto P, Palojoki E, Bäcklund T, Saraste A, Tikkanen I, Voipio-Pulkki LM, Pulkki K et al. Expression of heme oxygenase-1 in response to myocardial infarction in rats. J Mol Cell Cardiol. 2002;34:1357-65.
31. Lee DC, Lee MO, Clifford DH. Cardiovascular effects of moxibustion at Jen Chung (Go-26) during halothane anesthesia in dogs. Am J Chin Med (Gard City N Y). 1975;3:245-61.
32. Lee WH, Kang S, Vlachos PP, Lee YW. A novel in vitro ischemia/reperfusion injury model. Arch Pharm Res. 2009;32:421-9.
33. Li C and Zhen H. Design of the laser acupuncture therapeutic instrument. Conf Proc IEEE Eng Med Biol Soc. 2006;1:4107-10.
34. Lin HH, Chen YH, Chang PF, Lee YT, Yet SF, Chau LY. Heme oxygenase-1 promotes neovascularization in ischemic heart by coinduction of VEGF and SDF-1. J Mol Cell Cardiol. 2008;45:44-55.
35. Li P, Ayannusi O, Reid C, Longhurst JC. Inhibitory effect of electroacupuncture (EA) on the pressor response induced by exercise stress. Clin Auton Res. 2004;14:182-8.
36. Li P, Rowshan K, Crisostomo M, Tjen-A-Looi SC, Longhurst JC. Effect of electroacupuncture on pressor reflex during gastric distension. Am J Physiol Regul Integr Comp Physiol. 2002;283:R1335-45.
37. Li P, Tjen-A-Looi SC, Guo ZL, Fu LW, Longhurst JC. Long-loop pathways in cardiovascular electroacupuncture responses. Appl Physiol. 2009;106:620-30.
38. Litscher G, Rachbauer D, Ropele S, Wang L, Schikora D, Fazekas F, Ebner F. Acupuncture using laser needles modulates brain function: first evidence from functional transcranial Doppler sonography and functional magnetic resonance imaging. Lasers Med Sci. 2004;19:6-11.
39. Litscher G and Schikora D. Biological Effects Of Painless Laser Needle Acupuncture. Medical Acupuncure Journal. Vol 16, no.1.
40. Luna SP, Angeli AL, Ferreira CL, Lettry V, Scognamillo-Szabó M. Comparison of pharmacopuncture, aquapuncture and acepromazine for sedation of horses. Evid Based Complement Alternat Med. 2008;5:267-72.
41. Okinaga S and Shibahara S. Identification of a nuclear protein that constitutively recognizes the sequence containing a heat-shock element. Its binding properties and possible function modulating heat-shock induction of the rat heme oxygenase gene. Eur J Biochem. 1993;212:167-75.
42. Ondrejcakova M, Ravingerova T, Bakos J, Pancza D, Jezova D. Oxytocin exerts protective effects on in vitro myocardial injury induced by ischemia and reperfusion. Can J Physiol Pharmacol. 2009;87:137-42.
43. Pan PJ, Chan RC, Yang AH, Chou CL, Cheng YF, Chiu JH. Protective effects of preconditioned local somatothermal stimulation on neuromuscular plasticity against ischemia--reperfusion injury in rats. J Orthop Res. 2008;26(12):1670-4.
44. Perrella MA and Yet SF. Role of heme oxygenase-1 in cardiovascular function. Curr Pharm Des. 2003;9:2479-87.
45. Peterson SJ and Frishman WH. Targeting heme oxygenase: therapeutic implications for diseases of the cardiovascular system. Cardiol Rev. 2009;17:99-111.
46. Piper HM, Meuter K, Schäfer C. Cellular mechanisms of ischemia-reperfusion injury. Ann Thorac Surg. 2003;75:S644-8.
47. Sakamoto N, Kokura S, Okuda T, Hattori T, Katada K, Isozaki Y, Nakabe N et al. Heme oxygenase-1 (Hsp32) is involved in the protection of small intestine by whole body mild hyperthermia from ischemia/reperfusion injury in rat. Int J Hyperthermia. 2005;21:603-14.
48. Sammut IA and Harrison JC. Cardiac mitochondrial complex activity is enhanced by heat shock proteins. Clin Exp Pharmacol Physiol. 2003;30:110-5.
49. Shao ZH, Wojcik KR, Dossumbekova A, Hsu C, Mehendale SR, Li CQ, Qin Y et al. Grape seed proanthocyanidins protect cardiomyocytes from ischemia and reperfusion injury via Akt-NOS signaling. J Cell Biochem. 2009.
50. Stahnke T, Stadelmann C, Netzler A, Brück W, Richter-Landsberg C. Differential upregulation of heme oxygenase-1 (HSP32) in glial cells after oxidative stress and in demyelinating disorders. J Mol Neurosci. 2007;32:25-37.
51. Tjen-A-Looi SC, Li P and Longhurst JC. Processing Cardiovascular Information in the vlPAG during Electroacupuncture in Rats: Roles of Endocannabinoids and GABA. J Appl Physiol. 2009;106:1793-9.
52. Truini A, Romaniello A, Galeotti F, Iannetti GD, Cruccu G. Laser evoked potentials for assessing sensory neuropathy in human patients. Neurosci Lett. 2004;361:25-8.
53. Voegeli TS, Wintink AJ, Chen Y, Currie RW. Heat shock proteins 27 and 70 regulating angiotensin II-induced NF-kappaB: a possible connection to blood pressure control? Appl Physiol Nutr Metab. 2008;33:1042-9.
54. Walker MJ, Curtis MJ, Hearse DJ, Campbell RW, Janse MJ, Yellon DM, Cobbe SM et al. The Lambeth Conventions: guidelines for the study of arrhythmias in ischaemia infarction, and reperfusion. Cardiovasc Res. 1988;22:447-55.
55. Wang SB, Chen SP, Gao YH, Luo MF, Liu JL. Effects of electroacupuncture on cardiac and gastric activities in acute myocardial ischemia rats. World J Gastroenterol. 2008;14:6496-502.
56. West MB, Rokosh G, Obal D, Velayutham M, Xuan YT, Hill BG, Keith RJ et al. Cardiac myocyte-specific expression of inducible nitric oxide synthase protects against ischemia/reperfusion injury by preventing mitochondrial permeability transition. Circulation. 2008;118:1970-8.
57. Xu JJ and Wang YL. Propofol attenuation of hydrogen peroxide-mediated oxidative stress and apoptosis in cultured cardiomyocytes involves haeme oxygenase-1. Eur J Anaesthesiol. 2008;25:395-402.
58. Yamamoto H, Kawada T, Kamiya A, Kita T, Sugimachi M. Electroacupuncture changes the relationship between cardiac and renal sympathetic nerve activities in anesthetized cats. Auton Neurosci. 2008;144:43-9.
59. Yin H, Zhang J, Lin H, Qiao Y, Wang R, Lu H, Liang S. Effect of traditional Chinese medicine Shu-mai-tang on angiogenesis, arteriogenesis and cardiac function in rats with myocardial ischemia. Phytother Res. 2009;23:92-8.
60. Zhang M, Zhang BH, Chen L, An W. Overexpression of heme oxygenase-1 protects smooth muscle cells against oxidative injury and inhibits cell proliferation. Cell Res. 2002;12:123-32.
61. Zhang XY, Li WH, Wen TF, Li CH, Cheng Z, Zhang XL, Su XJ. [Clinical observation on injection of small dose of morphine into Neiguan (PC 6) for treatment of chest pain in the patient with acute myocardial infarction] Zhongguo Zhen Jiu. 2008;28:642-4.
62. Zhao J, Huang X, Tang W, Ren P, Xing Z, Tian X, Zhu Z et al. Effect of oriental herbal prescription Guan-Xin-Er-Hao on coronary flow in healthy volunteers and antiapoptosis on myocardial ischemia-reperfusion in rat models. Phytother Res. 2007;21:926-31.
63. Zhao L, Shen XY, Gao JP, Ding GH, Wei JZ, Deng HP, Wang L et al. Effect of different LLLT on pituitrin-induced bradycardia in the rabbit. Lasers Med Sci. 2006;21:61-6.
64. Zhen-Wei X, Jian-Le S, Qi Q, Wen-Wei Z, Xue-Hong Z, Zi-Li Z. Heme oxygenase-1 improves the survival of discordant cardiac xenograft through its anti-inflammatory and anti-apoptotic effects. Pediatr Transplant. 2007;11:850-9.
65. 施松杉,針灸對大白鼠敗血症之免疫調控與機轉之探討,國立陽明大學傳統醫學研究所碩士論文,1999
66. 童華雄,探討溫灸大白鼠體表穴位及相對應內臟器官熱休克蛋白七十之表現,國立陽明大學傳統醫學研究所碩士論文,2002
67. 鄒孟婷,針灸大白鼠內關穴對心肌保護之機轉探討,國立陽明大學傳統醫學研究所博士論文,2004
68. 杜怡樺,鴉片類受體在Baicalein對雞胚心肌細胞抗缺氧再灌流傷害的保護作用之角色,國立陽明大學傳統醫學研究所碩士論文,2005
69. 謝政橘,整合中西醫學對於大白鼠肝臟缺血再灌流損傷保護效果之研究,國立陽明大學傳統醫學研究所碩士論文,2005
70. 彭彥文,雷射針刺正常人的內關穴對心率變異度的影響與動物實驗模式之建立,國立陽明大學傳統醫學研究所碩士論文,2008
71. 王全權、陳海林、黃慧敏,複方丹參注射液治療冠心病心絞痛的療效觀察,時珍國醫國藥,2006;17
72. 張秋玲、楊勤,丹参心俞穴位注射對急性心肌梗塞大鼠的心梗面積的ET-1的影響,貴陽中醫學院學報,2002;24
73. 張登部、孫學全,艾條懸灸法治療冠心病138例臨床觀察,中醫雜誌,1991;32:35
74. 劉振義、劉勇,懸灸法治療冠心病62例臨床研究,中醫外治雜誌,1996;5:6
75. 沈雪勇、丁光宏、高建平,紅外激光針灸對垂體後葉素致家兔心動過緩的影響,中西醫結合學報,2006
76. 楊清容、林仁壽,新獸醫學針灸學第二冊,華香園出版社,台北,2009;pp.281
77. 林昭庚,針灸學,知音出版社,台北,2009
78. 林昭庚,中西醫病名對照大辭典第二冊,國立中國醫藥研究所,台北,2001,pp.910-19
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊