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研究生:黃思怡
研究生(外文):Shih-Yi Huang
論文名稱:肝癌復發轉移的機制探討及miR-34a扮演的角色
論文名稱(外文):Mechanism analysis on Hepatocellular carcinoma (HCC) recurrence and metastasis: Emphasize on miR-34a
指導教授:王學偉王學偉引用關係
指導教授(外文):Hsei-Wei Wang
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:微生物及免疫學研究所
學門:生命科學學門
學類:微生物學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:40
中文關鍵詞:肝癌小RNA-34a復發轉移
外文關鍵詞:Hepatocellular carcinoma (HCC)miR-34aRecurrenceMetastasis
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肝癌的發生率不論在全世界或在台灣皆為所有癌症中的前幾位,更因
為其容易復發及轉移的特性,造成死亡率居高不下。因此若能清楚了解肝癌的發生及復發或轉移的機轉,而能有良好的藥物治療標的,對於肝癌病人來說將是莫大的福祉。本研究由全轉錄體(whole transcriptome)中的microRNA與 mRNA兩個層面共同切入,期望能找到與肝癌復發或轉移相關的基因,進而對臨床預後及治療有所貢獻。在 microRNA 方面,首先我們觀察到在臨床檢體中 miR-34a 與肝癌復發及轉移的能力呈現負相關,接著在in vitro的實驗中藉由雙向的正反驗證來證實miR-34a的確能夠抑制肝癌細胞株的移動能力;並且建立了穩定持續表現 miR-34a 的 Mahlavu 細胞株,未來會在 in vivo 的情況下進一步驗證。在 mRNA 方面,我們將臨床組織檢體以及在Huh7細胞株中knock down miR-34a表現之後的total RNA進行 expression microarray,期望能夠找到與復發或轉移相關的基因同時也是 miR-34a 的下游目標基因,而能在未來做為藥物治療的標的。
Hepatocellular carcinoma (HCC) is the leading cause of malignant tumors in Taiwan, and its death rate is also high because of its high probability of recurrence and metastasis. It is very important to find the mechanisms of HCC recurrence and metastasis, and such new knowledge may help us to design new drugs for curing this terribly malignant cancer. We first focused on the level of miR-34a in clinical HCC tissues. We found there is a negative correlation between the expression level of miR-34a and the occurrence of primary HCC recurrence and metastasis. Secondly, we proved that miR-34a has the ability to inhibit the migration of HCC cell lines in vitro and established Mahlavu-34a cell line for in vivo experiment. Finally, we used expression microarray to find the target genes of miR-34a which are also related to HCC recurrence and
metastasis. This study will help to develop the effective drugs to cure hepatocellular carcinoma in the future. It will be a gift for those people who suffer from the pain of HCC.
1. 英文摘要………………………………………………………… 1
2. 中文摘要………………………………………………………… 2
3. 緒論……………………………………………………………… 3
4. 材料與方法……………………………………………………… 9
5. 結果………………………………………………………………16
6. 討論………………………………………………………………22
7. 參考文獻…………………………………………………………25
8. 圖表………………………………………………………………28
9. 附錄………………………………………………………………38
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