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研究生:韓欣純
研究生(外文):Shin-Chwen Han
論文名稱:乙型轉換生長因子對人類乳癌細胞玻尿酸合成�〞穛{的影響
論文名稱(外文):Effects of Transforming Growth Factor-beta on Hyaluronan Synthase Expression in Human Breast Carcinoma Cells
指導教授:王懷詩
指導教授(外文):Hwai-Shi Wang
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:解剖暨細胞生物學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:53
中文關鍵詞:玻尿酸合成酶玻尿酸乙型轉換生長因子乳癌
外文關鍵詞:Hyaluronan synthaseHyaluronanTGF-betabreast cancer
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乙型轉換生長因子(TGF-beta)為細胞分泌的一種多胜肽,可以調控細胞的增生、分化、轉移、細胞外間質沉積,也可以促進乳癌細胞的轉移能力增加;目前已知在乳癌細胞有過量的玻尿酸合成酶(Hyaluronan synthase, HAS)表現,造成玻尿酸(Hyaluronan)產生的量增加。由於過量的玻尿酸也會促進細胞的癌化和轉移能力增加,因此在這裡我們想要了解同樣會造成細胞轉移能力增加的玻尿酸和TGF-beta所引起細胞的反應是否有相關聯。在本論文主要研究三株人類乳癌細胞株:MCF7、MDA-MB-231及Hs578T,以即時聚合酶鏈鎖反應、西方點墨法、玻尿酸酵素免疫分析法來觀察TGF-beta刺激細胞後,細胞不同亞型的HAS:HAS1、HAS2及HAS3 mRNA和蛋白質的表現量以及細胞產生的玻尿酸量是否有改變。由結果顯示TGF-beta可以促進MCF7細胞株 HAS1、HAS2與Hs578T細胞株HAS2的表現量增加,造成細胞產生的玻尿酸增加,推論TGF-beta促進乳癌細胞轉移能力的增加可能與TGF-beta引起HAS1、HAS2增加,使得大分子玻尿酸合成增加有關。
Transforming growth factor (TGF-beta) is a secreted polypeptide and regulates cellular functions, such as cell proliferation, differentiation, migration and extracellular matrix (ECM) deposition. Also, TGF-beta can promote breast carcinoma cells migration. There is already known that overexpression of hyaluronan synthase in breast carcinoma cells will synthesize high level of hyaluronan. Because high level of hyaluronan will also promote tumorigenicity and metastasis of breast carcinoma cells, we want to understand that if there is a correlation between hyaluronan and TGF-beta. We studied in three human breast carcinoma cell lines : MCF7, MDA-MB-231, Hs578T. We used real timr-PCR, western blot, HA ELISA to study TGF-beta- induced different isoform hyaluronan synthase and hyaluronan expression in transcription level and protein level. We demonstrated that TGF-beta increase HAS1, HAS2 expression and hyaluronan synthesis in MCF7 cell line and increase HAS2 expression and hyaluronan synthesis in Hs578T cell line. These results suggest that TGF-beta promoted breast carcinoma cells migration may be related to TGF-beta induced HAS1, HAS2 expression and produces high molecular weight hyaluronan in breast carcinoma cell line.
中文摘要…………………………………………………………………iv
英文摘要………………………………………………………………… v
目錄………………………………………………………………………vi
第一章緒論………………………………………………………………1
壹、乳癌(Breast carcinoma)………………………………………1
貳、玻尿酸與玻尿酸合成酶��
(Hyaluronan and Hyaluronan synthase) ……………………………2
�礡B乙型轉換生長因子
(Transforming growth factor-beta,TGF-beta��)…………………7肆、研究目的……………………………………………………………10
第二章 材料與方法……………………………………………………11
壹、儀器設備…………………………………………………………11
貳、實驗材料與試藥……………………………………………………13
�礡B培養液與實驗用溶液的配製………………………………………17
肆、實驗方法……………………………………………………………20
第三章 實驗結果………………………………………………………26
第四章 討論……………………………………………………………29
參考文獻………………………………………………………………33
附錄………………………………………………………………………50
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