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研究生:林孟篁
研究生(外文):Meng-Huang Lin
論文名稱:檳榔素及半天筍(檳榔莖)萃取物之活體效應探討
論文名稱(外文):The In Vivo Effects of Arecoline and the Extract of the Budding Stem of Areca Catechu L.
指導教授:劉宗榮劉宗榮引用關係
指導教授(外文):Tsung-Yun Liu
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:環境與職業衛生研究所
學門:工程學門
學類:環境工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:62
中文關鍵詞:半天筍活體效應檳榔素
外文關鍵詞:the budding stem of Areca Catechu L.in vivo effectArecoline
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半天筍為檳榔樹生長點的嫩莖,一般可用來作為食材入菜,由於台灣曾有報導民眾攝食半天筍引發中毒現象,引起食用安全上的疑慮。一般認為半天筍中毒是由所含檳榔素所引起,但缺乏直接證據。為了瞭解及預防半天筍可能帶來的危害,本實驗分別以80%丙酮與0.1%醋酸萃取半天筍,將所得萃取液以高效能液相層析儀,分析內含主要四種檳榔生物鹼的含量。另一方面使兩組SD大鼠分別餵食不同萃取方式的半天筍萃取物,測量餵食後30分鐘大鼠心跳,血壓值的變化,並收集血液分析餵食半天筍萃取物後大鼠血中的生物鹼濃度,比較餵食前後數值的變化。
本研究結果顯示,餵食大鼠劑量2g/kg 不同萃取方式的半天筍萃取物後,無論公、母鼠,在心跳,血壓部分皆有所影響。以母鼠餵食後比對餵食前心跳、血壓變化有顯著性。在餵食半天筍萃取物後短時間收集血液樣本中並未測到檳榔素的存在。因此食用半天筍所引發的人體不適現象由來,未來仍須進一步再做研究。
The budding stem of Areca Catechu Linn. is served as a food in Taiwan. However, poison cases have been reported following the consumption of this food, and raise the concern of the safety of using this as food. It is suspected that arecoline in Areca Catechu Linn. might be responsible for the toxicity, but lacks direct evidence. In order to clarify and prevent the harm of eating this food stuff, this study used two extractive methods :80% acetone and 0.1% acetic acid, to extract the budding stem of Areca Catechu Linn. The alkaloids in this extracts was determined and quantified by High Performance Liquid Chromatography(HPLC). On the other hand, the extracts was gavaged to Sprague Dawley rats, and the heart rate, blood pressure were determined thirty minutes later. In addition, the blood arecoline levels were also determined by HPLC
This results demonstrated that the extract of the budding of Areca Catechu Linn. did induce heart rate and blood pressure in both male and female rats at 2 g/kg, and the effects were more significant in female rats. However, the arecoline was not detected in rats 30 minutes after the dosing. Therefore, the major cause of poising of the budding stem of Areca Catechu Linn. need further study.
中文摘要 I
Abstract II
壹、緒論 1
貳、實驗動機與目的 7
參、實驗材料與方法 8
肆、實驗結果 19
伍、討論 25
陸、結論 29
柒、參考文獻 30
捌、實驗圖表 33
1. Gupta P, Warnakulasuriya S. Global epidemiology of areca nut usage. Addict Biol 2002; 7: 77-83.
2. Ko Y, Chiang T, Chang S, Hsieh S. Prevalence of betel quid chewing habit in Taiwan and related sociodemographic factors. J Oral Pathol Med 1992; 21: 261-4.
3. 邱重榕, 陳建仁. 口腔黏膜下纖維化之流行病學. 臺灣公共衛生雜誌 2001; 20: 84-92.
4. IARC. Betel-quid and areca-nut chewing and some areca-nut derived nitrosamines. IARC Monogr Eval Carcinog Risks Hum 2004; 85: 1-334.
5. IARC. Tobacco habits other than smoking; betel-quid and areca-nut chewing; and some related nitrosamines. IARC Working Group. Lyon, 23-30 October 1984. IARC Monogr Eval Carcinog Risk Chem Hum 1985; 37: 1-268.
6. Ko Y, Huatig Y, Lee C, Chen M, Litt L, Tsai C. Betel quid chewing, cigarette smoking and aicohoi consumption reiated to orai cancer in Taiwan. J Oral Pathol Med 1995; 24: 450-3.
7. 行政院農委會. [cited; Available from: http://www.coa.gov.tw/search_wg.php
8. 國民健康局 行. [cited; Available from: http://www.bhp.doh.gov.tw/BHPnet/Portal/PressShow.aspx?No=200906060001
9. Ranadive K, Gothoskar S, Rao A, Tezabwalla B, Ambaye R. Experimental studies on betel nut and tobacco carcinogenicity Int J Cancer 1976; 17: 469-76.
10. Sundqvist K, Liu Y, Nair J, Bartsch H, Arvidson K, Grafstrom R. Cytotoxic and genotoxic effects of areca nut-related compounds in cultured human buccal epithelial cells. Cancer Res 1989; 49: 5294-8.
11. Sarbani Giria, Kristopher W. Krausza, Jeffrey R. Idlec, Gonzalez FJ. The metabolomics of (±)-arecoline 1-oxide in the mouse and its formation by human flavin-containing monooxygenases . Biochem Pharmacol 2007; 73: 561-73.
12. 王進崑, 撰. 檳榔嚼塊中興奮性成分之研究及替代品的研製 [博士論文]: 國立臺灣大學食品科技研究所,
臺北; 1993.
13. Rand M, Varma B. The effects of cholinomimetic drugs on responses to sympathetic nerve stimulation and noradrenaline in the rabbit ear artery. Br J Pharmacol 1970; 38: 758-70.
14. Yusuf H, Yong S. Oral submucous fibrosis in a 12-year-old Bangladeshi boy: a case report and review of literature. Int J Paediatr Dent 2002; 12: 271-6.
15. Patil P, Stearns R. Mechanism of vascular relaxation by cholinomimetic drugs with special reference to pilocarpine and arecoline. J Ocul Pharmacol Ther 2002; 18: 25-34.
16. Jeng J, Kuo M, Hahn L, Kuo M. Genotoxic and non-genotoxic effects of betel quid ingredients on oral mucosal fibroblasts in vitro. J Dent Res 1994; 73: 1043-9.
17. Wang C, Hwang L. Phenolic compounds of betel quid chewing juice. Food Sci 1993; 20: 458–71.
18. Nair U, Friesen M, Richard I, MacLennan R, Thomas S, Bartsch H. Effect of lime composition on the formation of reactive oxygen species from areca nut extract in vitro. Carcinogenesis 1990; 11: 2145-8.
19. Wenke G, Hoffmann D. A study of betel quid carcinogenesis. 1. On the in vitro N-nitrosation of arecoline. Carcinogenesis 1983; 4: 169-72.
20. Prokopcuka B, Brunneman KD, Bertinoto P, Hoffmann D. The role of N-(nitrosodimethylamine) propionitrile in betel- quid carcinogenesis. IARC Sci Publ 1987: 470-3.
21. Chu N. Effects of betel chewing on the central and autonomic nervous systems. J Biomed Sci 2001; 8: 229-36.
22. Lodge D, Johnston G, Curtis D, Brand S. Effects of the Areca nut constituents arecaidine and guvacine on the action of GABA in the cat central nervous system. Brain Res 1977; 136: 513-22.
23. Ghelardini C, Galeotti N, Lelli C, Bartolini A. M1 receptor activation is a requirement for arecoline analgesia Il Farmaco 2001; 56: 383-5.
24. Yang Y, Chang K, Chen C, Chiu T. Arecoline excites rat locus coeruleus neurons by activating the M2-muscarinic receptor The Chinese Journal of Physiology 2000; 43: 23-8.
25. Xie D, Chen L, Liu C, Zhang C, Liu K, Wang P. Arecoline Excites the Colonic Smooth Muscle Motility via M3 Receptor in Rabbits Chin J Physiol 2004; 47: 89-94.
26. Christie J, Shering A, Ferguson J, Glen A. Physostigmine and arecoline: effects of intravenous infusions in Alzheimer presenile dementia. The British Journal of Psychiatry 1981; 138: 46-50.
27. Pichini S, Pellegrini M, Pacifici R, et al. Quantification of arecoline (areca nut alkaloid) in neonatal biological matrices by high-performance liquid chromatography/electrospray quadrupole mass spectrometry. Rapid Commun Mass Spectrom 2003; 17: 1958-64.
28. Pellegrini M, Marchei E, Rossi S, et al. Liquid chromatography/electrospray ionization tandem mass spectrometry assay for determination of nicotine and metabolites, caffeine and arecoline in breast milk. Rapid Commun Mass Spectrom 2007; 21: 2693-703.
29. Marchei E, Durgbanshi A, Rossi S, Garcia-Algar O, Zuccaro P, Pichini S. Determination of arecoline (areca nut alkaloid) and nicotine in hair by high-performance liquid chromatography/electrospray quadrupole mass spectrometry. Rapid Commun Mass Spectrom 2005; 19: 3416-8.
30. Sharan R, Wary K. Study of unscheduled DNA synthesis following exposure of human cells to arecoline and extracts of betel nut in vitro. Mutation Research 1992; 278: 271-6.
31. Cox S, Piatkov I, Vickers E, Ma G. High-performance liquid chromatographic determination of arecoline in human saliva. J Chromatogr 2004; 1032: 93-5.
32. Jiu Li H, McLEISH* MJ. High-performance liquid chromatographic determination of the alkaloids in betel nut. Journal of Chromatography
1989; 475: 477-50.
33. Qiu P, Chen X, Lin L, Ai C. Simultaneous determination of five toxic alkaloids in body fluids by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry. J Chromatogr B 2008; 875: 471-7.
34. Mahoney G, Al-Delaimy W. Measurement of nicotine in hair by reversed-phase high-performance liquid chromatography with electrochemical detection. Journal of Chromatography B: Biomedical Sciences and Applications 2001; 753: 179-87.
35. Torres A, Davila D, Gottberg C, Donis J, Arata de Bellabarba G, Ramoni-Perazzi P. Heart rate responses to a muscarinic agonist in rats with experimentally induced acute and subacute chagasic myocarditis. Rev Inst Med Trop Sao Paulo 2000; 42: 219-24.
36. Yen A, Chiu Y, Chen L, et al. A population-based study of the association between betel-quid chewing and the metabolic syndrome in men. Am J Clin Nutr 2006; 83: 1153-61.
37. Barnes J, Roberts F. Central effects of muscarinic agonists and antagonists on hippocampal theta rhythm and blood pressure in the anaesthetised rat. Eur J Pharmacol 1991; 195: 233-40.
38. Giri S, Idle J, Chen C, Zabriskie T, Krausz K, Gonzalez F. A Metabolomic Approach to the Metabolism of the Areca Nut Alkaloids Arecoline and Aracaidine in the Mouse. Chem Res Toxicol 2006; 19: 818-27.
39. Nery R. The metabolic interconversion of arecoline and arecoline 1-oxide in the rat. Biochem J 1971; 122: 503.
40. Hussain M, Mollica J. Intranasal absorption of physostigmine and arecoline J Pharm Sci 1991; 80: 750-1.
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