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研究生:賴韻竹
研究生(外文):Yun-Zhu Lai
論文名稱:利用磁振造影對比劑量化骨髓間質幹細胞之數量
論文名稱(外文):A Method to Quantify Marrow Mesenchymal Stem Cells by Using Contrast Agent in Magnetic Resonance Imaging
指導教授:朱唯勤朱唯勤引用關係
指導教授(外文):Woei-Chyn Chu
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:醫學工程研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:54
中文關鍵詞:對比劑磁振造影骨髓間質幹細胞
外文關鍵詞:Contrast agentMagnetic Resonance Image (MRI)Mesenchymal stem cells (MSCs)
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  • 收藏至我的研究室書目清單書目收藏:0
近年來幹細胞移植治療研究已應用在許多不同疾病上,而移植後需要觀察並追蹤其細胞是否到達組織損傷部位。為了實現非侵入式的觀察方式,可將幹細胞結合了磁振造影對比劑,利用磁振造影技術就可以對細胞做非侵入式的追蹤,除了可以知道細胞所在位置,還可以觀察其數量,如此一來,對於細胞移植研究上有更大助益。
在本研究中,使用Resovist磁振造影對比劑標定骨髓間質幹細胞,由於幹細胞移植進入體內組織時,並非一定聚集在同一位置中,可能會分佈在同一組織上,因此本研究分別將不同數量幹細胞平均分散並固定於明膠 (gelatin) 中,並取得磁振造影之影像強度做比較,找出細胞數量與影像強度值的相關性。在實驗結果中,我們發現影像強度隨幹細胞數量增加而遞減,由於使用一定細胞數量分散在固定體積中,因此若將結果應用在細胞體內移植上,便可藉由影像強度推估出所在位置之幹細胞數量。
In the last few years, the stem cell treatment already had many research applications applied to different diseases. After stem cell transplantation, we need to trace the stem cells whether they have arrived at the damage tissues. In order to achieve noninvasive observation, we label the stem cells with contrast agent, using Magnetic Resonance Image (MRI) to monitor and quantify the homing of the labeled stem cells.

In this research, we use the Resovist T2 contrast agent to label the marrow mesenchymal stem cells (MSCs). In vivo cell transplantation, the cells may not stay in the identical position, so we mixed different quantity cells and fixed them evenly in gelatin. We then compared their MRI intensities with the labeled-cell quantity. We discovered the cell quantity was inversely proportional to the MRI intensity value. Based on this result, we can estimate cell quantity in a position by its MRI intensity value.
目錄
摘要
Abstract
致謝
目錄
表目錄
圖目錄
第一章 幹細胞
1.1 幹細胞簡介
1.2 幹細胞來源
1.3 間質幹細胞 (Mesenchymal stem cells)
1.3.1 間質幹細胞的來源
1.3.2 間質幹細胞的分化能力
1.3.3 間質幹細胞之標定
第二章 磁振造影對比劑 (MRI contrast agent)
2.1 磁振造影 (magnetic resonance imaging)
2.2 遲緩 (relaxation)
2.3 對比劑原理
2.3.1 Positive Enhancer
2.3.2 Negative Enhancer
2.4 相關文獻回顧
第三章 研究動機與目的
第四章 實驗材料與方法
4.1 骨髓間質幹細胞取得與培養
4.1.1 取得大鼠骨髓間質幹細胞
4.1.2 間質幹細胞之培養
4.1.3 細胞之繼代培養
4.1.4 細胞之冷凍保存與解凍
4.2 細胞計數
4.3 標定間質幹細胞
4.3.1 標定方式
4.3.2 普魯士藍染色 (Prussian blue staining)
4.3.3 細胞增生測試
4.4 磁振造影
4.4.1 標定與未標定細胞之MR影像
4.4.2 明膠製作
4.4.3 標定細胞之T2弛緩時間 (T2 relaxation time)
4.4.4 不同細胞數量與影像強度
第五章 實驗結果
5.1 間質幹細胞培養
5.2 細胞標定
5.2.1 細胞普魯士藍染色 (Prussian blue staining)
5.2.2 細胞增生測試
5.3 細胞磁振造影
5.3.1 標定與未標定細胞之磁振造影
5.3.2 T2弛緩時間 (T2 relaxation time)
5.3.3 不同細胞數量與影像強度
第六章 結果討論
6.1 間質幹細胞培養
6.2 不同標定時間之細胞普魯士藍染色
6.3 細胞增生情形
6.4 未標定與標定細胞之磁振造影結果比較
6.5 不同濃度對比劑標定細胞之影像強度變化
6.6 不同數量細胞之影像強度值
第七章 結論
參考文獻
8 參考文獻
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