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研究生:張哲維
研究生(外文):Zhe-Wei Chang
論文名稱:拉曼散射光譜於尿液細胞學上的診斷
論文名稱(外文):Raman Spectroscopy Assisted Diagnosis in Urine Cytology
指導教授:江惠華江惠華引用關係
指導教授(外文):Huihua Kenny Chiang
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:醫學工程研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:81
中文關鍵詞:拉曼光譜尿液細胞學
外文關鍵詞:raman spectroscopyurine cytology
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尿液細胞學檢查(urine cytology)是目前最廣泛使用在膀胱癌上檢測方法,但此種以細胞型態判讀為主的效果並不完美,並且對於低度變異的細胞會常常判別不出來,而影響結果。
本研究針對尿液細胞學上在膀胱上皮癌細胞(transitional cell carcinom 38個)與正常細胞(33個)判斷上進行拉曼光譜分析,輔助病理科人員的判斷。。樣本是採用染色尿液抹片,針對其細胞核量測拉曼訊號。拉曼光譜峰值顯示1000cm-1(phenylalanine band)、725cm-1 (腺嘌呤-adenine, CH2 deformation)、538cm-1 (腺嘌呤-adenine, S-S鍵 )。其特徵峰值面積積分或其強度會隨著細胞變異後而減小。
將拉曼光譜量測結果依病理結果分類後加以比較,並且套入Partial Least Squares (PLS)程式分析,其靈敏度(sensitivity)高達97%,特異度(speciticity)達到100%。而用725/100cm-1面積積分比值的結果以ROC曲線 (Receiver Operating Characteridtic)的sensitivity 81.8%,specificity 90.3%。此實驗證實拉曼對於尿液細胞學檢查上有良好的準確率。
Urine cytology (UC) is the most widely used in the detection of bladder cancer. In addition, especially for low-grade lesions, UC is of limited value beacase of operator dependency and the low sensitivity, and would affect the results.
In this study, urine cytology for bladder epithelial cells in the (transitional cell carcinom 38) of normal cells (33) to determine on Raman Spectroscopy, assisted Pathology of person to determine. Urine sample is stained smears, the nucleus for its Raman signal measurement. Raman spectra show the peak 1000cm-1 (phenylalanine band), 725cm-1 (adenine, CH2 deformation), 538cm-1 (adenine, S-S ).
The peak area of integration or its intensity variation decreased with the grade of cells. Raman spectroscopy to measure the results sorted according to pathology results are compared, and set into the Partial Least Squares (PLS) program analysis, the sensitivity as high as 97% and specificity 100%.725/100cm-1 area using the ratio of the results points to ROC curve (Receiver Operating Characteridtic) the sensitivity 81.8%, specificity 90.3%. Raman for this experiment confirmed that urine cytology has a good accuracy.
致謝 I
Abstract II
中文摘要 III
第一章序論 1
1.1 研究背景 1
1.2. 膀胱癌 3
1.2.1前言 3
1.2.2膀胱構造與位置 5
1.2.3膀胱癌的種類 6
1.2.4膀胱癌的分化與期別 7
1.2.5膀胱癌之診斷 9
1.3 Papanicolaou Stain 原理 12
1.4 文獻回顧 15
1.5 研究目的 17
1.6論文架構 18
第二章理論基礎 19
2.1 拉曼散射 19
2.1.1 拉曼散射簡史 19
2.1.2 拉曼散射原理 21
2.2部分最小平方法 (Partial Least Squares:PLS) 28
第三章 研究架構與方法 31
3.1 拉曼散射光譜儀 31
3.2樣本來源及實驗流程 33
3.2尿液抹片製作流程 34
3.3 Papanicolaou Stain 流程 35
3.4 資料分析 37
3.4.1宇宙射線移除 37
3.4.2 背景的減除 37
3.4.3 光譜標準化 39
第四章 結果與討論 40
4.1.1光漂白(Fluorescence photobleaching) 40
4.1.2 光漂白時間與背景螢光關係 42
4.2 積分時間的評估 44
4.3染劑光譜與單顆染色尿路上皮細胞光譜比較 45
4.4膀胱上皮細胞型態上的分類 46
4.5 膀胱上皮細胞光譜 49
4.6 PLS 分析結果 51
4.7尿路上皮細胞特徵峰值面積積分的關係 55
4.8 Special case test 58
4.9未知細胞測試 61
第五章 結論與未來展望 63
5.1結論 63
5.2未來展望 64
參考文獻 65
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