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研究生:周振宇
研究生(外文):Chen-Yui Chou
論文名稱:60Hz電磁場刺激對星狀細胞的影響
論文名稱(外文):Effects of 60 Hz Electromagnetic Field Stimulation on Astrocytes
指導教授:陳敏弘陳敏弘引用關係孫瑞昇孫瑞昇引用關係
指導教授(外文):Ming-Hong ChenJui-Sheng Sun
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:醫學工程研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:英文
論文頁數:46
中文關鍵詞:電磁場星狀細胞發炎物質
外文關鍵詞:NOEMFAstrocyteInflammatory cytokine
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In modern society, the use of electricity is so universal that it is hard to prevent exposure to extremely low electromagnetic field (EMF) produced by power lines and other electrical appliances. Recently, Epidemiological studies have suggested that exposure to electromagnetic fields (EMF) may be associated with an increased incidence of degenerative disease and tumor formation. Some studies have shown that EMF stimulations enhance the growth of astrocytoma. Astrocytomas are primary central nerve system(CNS) tumors. For this reason, we suggest EMF stimulations may cause negative bio-effects on CNS. However, further cellular or molecular mechanisms involved in these effects of EMF stimulations to astrocyte are not known. The purpose of this study was to evaluate the effects of EMF on astrocytes such as the level of reactive oxygen species(ROS), gene expression of cytokines in stimulated astrocytes. Furthermore, we observe the molecular responses of astrocytes exposed to electromagnetic fields and evaluate whether EMF stimulations play roles in the pathophysiological processes. In our experiments, bioactivities of astrocytes decreased after EMF stimulation. Besides, the NO concentration in culture solution also tended to increase under long duration of EMF stimulation. Therefore, our results suggested that 60 Hz / 2 mT EMF stimulation had influences on bioactivities, production of free radicals and cytokines of astrocytes in vitro which might contribute to the degenerative processes and pathological conditions of the central nervous system.
In modern society, the use of electricity is so universal that it is hard to prevent exposure to extremely low electromagnetic field (EMF) produced by power lines and other electrical appliances. Recently, Epidemiological studies have suggested that exposure to electromagnetic fields (EMF) may be associated with an increased incidence of degenerative disease and tumor formation. Some studies have shown that EMF stimulations enhance the growth of astrocytoma. Astrocytomas are primary central nerve system(CNS) tumors. For this reason, we suggest EMF stimulations may cause negative bio-effects on CNS. However, further cellular or molecular mechanisms involved in these effects of EMF stimulations to astrocyte are not known. The purpose of this study was to evaluate the effects of EMF on astrocytes such as the level of reactive oxygen species(ROS), gene expression of cytokines in stimulated astrocytes. Furthermore, we observe the molecular responses of astrocytes exposed to electromagnetic fields and evaluate whether EMF stimulations play roles in the pathophysiological processes. In our experiments, bioactivities of astrocytes decreased after EMF stimulation. Besides, the NO concentration in culture solution also tended to increase under long duration of EMF stimulation. Therefore, our results suggested that 60 Hz / 2 mT EMF stimulation had influences on bioactivities, production of free radicals and cytokines of astrocytes in vitro which might contribute to the degenerative processes and pathological conditions of the central nervous system.
Contents
Abstract…………………………………………………………….... Ι
Contents……………………………………………………………… .II
Tablec contents………..…………… ………………………………. IV
Figure contents………………………………………………………. .V
Chapter 1 Introduction……………………………………………… .1
1-1 Background…….…………………………………………… .1
1-2 Study aim ……...……………………………………………… .3
Chapter 2 Theoretical Basis………………..……………………….. .4
2-1 Electromagnetic Fields……………………………………... .4
2-2 Neuroglia………………………………………………….... .7
2-3 Nitric oxide (NO)………………………………………...… 12
2-4 Inflammatory reactions………………………...................... 13
2-5 Interleukins………………………………..……………….. 14
2-6 Tumor necrosis factor-α……………………………………. 16
Chapter 3 Meterials and Methods………………………………...... 17
3-1 Matrerials…………………………………………………... 17
3-2 Methods……………………………………………………. 21
3-2-1 Reagents manufacture………………………………… 21
3-2-2 Cell culture……………………………………………. 23
3-2-3 Electromagnetic field (EMF) stimulation system…….. 26
3-2-4 MTT assay…………..………………………………... 27
3-2-5 Griess reaction (NO)…………………………………. 29
3-2-6 RT-PCR…………………………………………..…… 30

Chapter 4 Results and Discussions………………………………… 35
4-1 MTT assay……….………………………………………… 35
4-2 NO assay ……...……………………………………………… 36
4-3 Effects of EMF stimulation on IL-1β mRNA expression…. 38
4-4 Effects of EMF stimulation on TNF-α mRNA expression... 40
4-5 Effects of EMF stimulation on IL-6 mRNA expression…… 41
Chapter 5 Conclusion and future work…………………………….. 42
Chapet 6 References.......................................................................... 43
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