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研究生:張雅筑
研究生(外文):Ya-Chu Chang
論文名稱:植物抗病基因資料庫之建置與序列特徵分析
論文名稱(外文):A Database for Plant Disease Resistance Genes:Database Construction and Sequence Motif Analysis
指導教授:趙雅婷趙雅婷引用關係
學位類別:碩士
校院名稱:元智大學
系所名稱:生物科技與工程研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:38
中文關鍵詞:疾病抵抗植物資料庫
外文關鍵詞:disease resistanceplantdatabase
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研究植物抗病基因不但有助於我們了解植物抵抗病蟲害之制,其研究成果更有用於降低病蟲害所引起的農產損失。由於植物疾病抵抗基因在育種研究以及植物病蟲害管理具有相當的重要性,本研究收集經過實驗驗證的植物抗病基因資料建構成資料庫,以方便使用者分析序列樣式。本資料庫包含抗病基因之核酸與蛋白質序列、染色體位置、功能域與序列特徵等資料,並提供查詢、分析工具以及抗病基因之序列比對。本研究所提供的資訊可望應用於協助辨識未知的抗病蛋白質序列,增進我們對於植物抗病基因結構之了解。
Many studies have revealed the structures of a number of plant disease-resistance genes through gene cloning, PCR amplification with degenerate primers, and genomic approaches. Most plant resistance gene products contain distinctive sequence features. Because plant disease-resistance genes are of paramount importance to resistance breeding program and disease control, we constructed a database for studying the sequence features and structural motifs of plant disease resistance genes. Our database provides a collection of experimentally validated plant disease-resistance genes, their sequence records, functional domain, and sequence motifs as well. The database for plant disease-resistance genes will help researchers to explore previous research results more efficiently, and will expand knowledge of disease-resistance related functional domain.
目錄
中文摘要III
英文摘要IV
誌謝V
目錄VI
圖目錄VIII
表目錄IX
1.研究背景1
1.1導論1
1.2研究動機與目的2
2.前人研究3
2.1 抗病基因的種類 3
2.2植物資料庫5
3. 資料庫系統之設計理念及系統架構7
3.1 系統設計目標7
3.2 系統環境8
3.3 系統資料庫結構說明9
4. 抗病基因資料庫的分析11
4.1資料收集11
4.2資料分析及統計12
5. 抗病基因資料庫的設計17
5.1資料庫的功能介紹式17
5.2資料庫的介面介紹20
5.3資料庫的規劃23
5.4使用MYSQL實做資料庫25
參考文獻30
附錄33
附錄一、實驗驗證抗病基因當中的PFAM DOMAIN33
附錄二、物種表37

圖目錄
圖1、植物抗病基因資料庫架構圖10
圖2、物種比例表12
圖3、實驗驗證抗病基因當中常見的PFAM DOMAIN13
圖4、阿拉伯芥及水稻出現常見抗病相關的DOMAIN數據15
圖5、網頁目錄選單20
圖6、基因資料21
圖7、基因詳細資料21
圖8、特徵序列的位置22
圖9、阿拉伯芥五條染色體上抗病基因的位置22
圖10、GENE_SEARCH資料庫畫面 28
圖11、SEARCH資料庫畫面28
圖12、MOTIFS資料庫畫面29
圖13、STRUCT資料庫畫面29

表目錄
表1、REFGENE在資料庫中欄位格式9
表2、常見抗病相關的DOMAIN14
表3、常見抗病相關的DOMAIN與TM及CC作組合16
表4、資料庫資料內容24
參考文獻
于世慶(2007),植物抗病相關之蛋白質序列特徵分析,元智大學生物科技暨生物資訊研究所碩士論文。
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