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研究生:林裕誠
研究生(外文):Yu-Cheng Lin
論文名稱:以薄膜過濾程序自醱酵液中純化回收靈菌紅素
論文名稱(外文):Purification and Recovery of Prodigiosin from Fermentation Broths Using Membrane Filtration Processes
指導教授:莊瑞鑫莊瑞鑫引用關係
指導教授(外文):Ruey-Shin Juang
學位類別:碩士
校院名稱:元智大學
系所名稱:化學工程與材料科學學系
學門:工程學門
學類:綜合工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:80
中文關鍵詞:靈菌紅素醱酵液超過濾
外文關鍵詞:ProdigiosinFerment brothUltrafiltration
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本論文主要探討利用混合薄膜超過濾程序,自醱酵處理液中分離純化靈菌紅素,為了提高靈菌紅素的純度,尋找薄膜過濾之最佳操作條件。實驗採用恆壓攪拌式進行超過濾,來探討在不同操作條件之阻隔率之變化。在超過濾程序中,因受到濃度極化和結垢現象的影響,導致濾速衰減。
當進料濾液濃度增加,阻隔率上升。在YM10下,濃度為300 mg/L與500 mg/L其阻隔率在3 ~ 5 %,1000 mg/L最高可達16 %;而在YM1下,300 mg/L與500 mg/L其阻隔率在40 ~ 50 %,而1000 mg/L最高可達77 %。而在經由純度分析下,由YM10進行過濾之靈菌紅素,其濾液端純度最高可提升2倍;而YM1則是濃縮端純度較高,最高可提升2倍。最後,利用串聯薄膜程序(PES10、YM1)來提高其純度。濾速理論分析方面,利用Birgitte等人在1997年提出濾餅過濾理論(Cake filtration theory),來推得在不同操作條件下薄膜結垢指標(Modified fouling index, MFI)。
In this study, separation and purification of prodigiosin from ferment broth by ultrafiltration using regenerated cellulose(YM) and polyethersulfone(PES) membranes with MWCO 10 and 1 kDa were studied. The YM 1 kDa membrane had a higher rejection of prodigiosin than that of the 10 kDa membrane at low concentration. The rejection of prodigiosin was about 50% and 5% for 1 kDa membrane, respectively. The results for rejection at high concentration were the same. The purities of prodigiosin were purification achieved 2 fold in the permeate. Finally, the two-step membrane filtration processes was proposed.
The MFI(Modified fouling index) were used to analyze flux behavior. It was shown that the flux decline was due to solute concentration polarization and fouling.
中文摘要 Ⅱ
Abstract III
誌謝 IV
目錄 V
表目錄 VIII
圖目錄 IX
符號說明 XI
第一章 緒論 1
1.1 前言 1
1.2 薄膜分離技術 2
1.2.1 薄膜分離簡介 2
1.2.2 薄膜分離技術的優缺點 4
1.2.3 超過濾之簡介 4
1.2.4 影響超過濾流量之主要因素 7
1.2.5 超過濾薄膜清洗 8
1.2.6 超過濾薄膜組件 9
1.2.7超過濾的應用 11
1.3 文獻回顧 13
1.3.1 Serratia屬及S. marcescens之生化特性 13
1.3.2 靈菌紅素 16
1.3.3 靈菌紅素之醱酵工程 18
1.3.4 靈菌紅素之分離 21
1.3.5 薄膜過濾生物產品 21
1.4 研究動機與方法 24
第二章 實驗部份 26
2.1 實驗藥品與儀器 26
2.1.1 實驗藥品 26
2.1.2 實驗儀器 27
2.2 實驗步驟 29
2.2.1 醱酵製備方法 29
2.2.2 靈菌紅素之分離純化 31
2.2.3 定性及定量分析 32
2.2.4 薄膜過濾方法 34
第三章 結果與討論 41
3.1定性定量分析探討 41
3.1.1 靈菌紅素FTIR分析 41
3.1.2 靈菌紅素表面張力分析 42
3.1.3 靈菌紅素Zeta分析 42
3.2 以恆壓式攪拌系統分離純化靈菌紅素 44
3.2.1 不同進料濃度對濾液通量與阻隔率影響 44
3.2.2 不同透膜壓差對濾液通量與阻隔率影響 48
3.2.3 不同攪拌速度對濾液通量與阻隔率影響 51
3.2.4 不同膜孔洞對濾液通量與阻隔率影響 52
3.2.5 不同膜材對濾液通量與阻隔率影響 54
3.2.6靈菌紅素之純度探討 56
3.3 以串聯式恆壓式攪拌系統分離純化靈菌紅素 59
3.3.1 串聯系統對濾液通量與阻隔率影響 59
3.3.2 串聯系統對靈菌紅素之純度影響 61
第四章 結垢理論分析 63
第五章 結論 72
參考文獻 73
自述 80
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