負壓隔離病房的設計除保持病房壓力低於周遭環境的負壓外，尚須能藉通風有效排除其中的病原體。本研究使用追蹤氣體濃度衰減法量測病房內各區間的有效空氣交換率，並配合殘留時間測定判斷病房各區間內的有害物移除效果。其中殘留時間以 50% 脈衝釋放追蹤氣體被排除所需的時間，t50%，做為指標。
本研究在病房內以上述兩種判定標準於病房內不同區間進行測試，歸納得到二者的相關性，並藉此探討負壓隔離病房內所可能存在的通風死角與異常氣流，這些現象都會妨礙病房內病原體的移除。同時也發現室內空調氣流也會對病原體排除效果造成顯著影響。

A negatively-pressurized isolation ward must maintain a ward pressure lower the ambient pressure. which prevent the pathogen from escaping into the environment. The ventilation in the ward is also required to remove the pathogen effectively.
This study measured the effective air change rate at different locations in a negatively-pressurized ward by the concentration decay of trace gas concentration. The resident times were also measured with trace gas released at different locations in the ward. The time for 50% of trace gas being exhausted, t50%, was used as an index.
The results of air change rate and t50% was compared at nine locations in the ward. The ventilation dead space and anomaly airflow, both would prevent the pathogen to be effectively removed, were able to be identified by the experimental results. It was found that the airflow generated by an air-condition in the ward may also have a significant effect on the pathogen-removing.

致謝i
摘要ii
Abstractiii
目錄iv
表目錄vi
圖目錄vii
第一章：緒論1
1-1 研究動機1
1-2 負壓隔離病房定義2
1-3 研究目的4
1-4 追蹤物質檢測技術5
1-5 文獻回顧8
第二章：研究方法9
2-1 初步檢測9
2-1-1 發煙管，煙霧可視化9
2-2追蹤氣體與設備10
2-3 空間劃分11
2-4 檢測方法12
第三章：結果與討論15
第四章：結論與建議 22
附錄23
參考文獻59

[1].ASTM (1993). Standard Test Methods for Determining Air Chang in a Single Zone by Means of a Tracer Gas Dilution. America Society of Testing and Materials E741-93: 625-640.
[2].ASHRAE (1999): AHRAE Handbook- HVAC Application. American Society of Heating, Refrigerating and Air-Conditioning.
[3].CDC (1994): Guideline for Preventing the Transmission of Mycobacterium Tuberculosis in Health-care Facilities. U.S. Dept. of Health and Human Services, Public Services, Centers for Disease Control and Prevention, Atlanta.
[4].Pavelchak, N., Palmer, W., DePersis, R. P., and London, M. A. (2002): A Simple and Inexpensive Method for Determing the Effective Ventilation Rate in a Negatively Pressurized Room Using Airborne Particles as a Tracer. Applied Occupation and Environmental Hygiene. 17 (10), 704-710.
[5].Sandberg M, Sjoberg M (1983) “The use of the moments for assessing air quality in ventilated rooms. “ Bldg. Envir., 181-197.
[6].陳友剛（2007）“負壓區間有害物移除性能與換氣率關係探討”，國科會研究計畫 96-2221-E-309-007。
[7].陳友剛（2005）“負壓隔離病房通風性能檢測技術的探討“，國科會研究報告93-2815-C309-017。
[8].勞研所（1995）：“作業環境有害物質採樣分析技術研發—作業場所有害氣體擴散測定技術”，勞委會勞工安全衛生研究所研究報告，IOSH84-A301。
[9].陳友剛（2003）：“吸菸室與禁菸區之區隔及空調設置規範及查核標準”，衛生署國民健康局菸害防制研究計畫（BHP-92-Anti-Tobacco-A901）期中報告。
[10].蕭可法（2007）：“負壓隔離病房通風性能檢測技術的探討”，長榮大學職業安全與衛生研究所。
[11].行政院衛生署疾病管制局（2003），嚴重急性呼吸道症候群（SARS）資訊網，http://www.cdc.gov.tw/sars/。
[12].行政院衛生署疾病管制局,行政院勞委會勞研所（2006），負壓隔離病房標準作業手冊。