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論文名稱(外文):Apple Polyphenols Decelerate Adipocyte Differentia-tion and Reduce Fat Storage in Experimental Obesity
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The global risk of overweight/obesity as well as the high incidence of diabetes, cardiovascular disease, and specific cancers has been recognized to associate with chronic diseases. Previous studies showed that visceral fat accumulation induces metabolic syndrome associated with insulin resistance, causing lifestyle-related diseases such as diabetes, hyperlipidemia, and hypertension, leading to arteriosclerosis. In some clinical studies reveals apple polyphenols (AP) can regulate fat metabolism in healthy subjects with high body mass index. In this study, we tried to clarify the effects and mechanisms of AP in an experimental obesity model. In in vitro study, the effect of AP inhibiting 3T3-L1 differentiation was investigated. In in vivo study, a high fat diet (0.2% of cholesterol and 10 % of corn oil) was fed to hamsters for 8 weeks to induce obesity. The results showed the AP reduced the level of 3T3-L1 cell differentiating to adipocyte. AP also reduced the body weight, pararenal and gonadal fat, the levels of triglyceride and total cholesterol in obese hamsters. . Our results show that AP possesses the ability to reduce the adipogenesis.

中文摘要 V
英文摘要 VI
壹、縮寫表 1
貳、序論 2
2.1肥胖定義 2
2.2脂肪組織與脂肪細胞 2
2.3脂肪細胞分化 3
2.4脂肪細胞分化研究模式 7
2.5分化調控因子 7
2.6脂質調節 10
2.7蘋果多酚 11
2.8多酚與脂肪細胞分化相關研究 12
叁、研究動機 14
肆、實驗架構 15
伍、實驗儀器 16
陸、實驗藥品 18
柒、實驗方法 20
7.1動物實驗 20
7.2細胞實驗 29
捌、藥品配製: 34
玖、結果 41
一、動物實驗結果 41
二、細胞實驗結果 44
拾、討論 48
附圖一、肥胖定義 67
附圖二、世界衛生組織對於肥胖之分類 68
附圖三、in vitro model of adipocyte differentiation 69
附圖四、3T3-L1 細胞分化流程表 70
附圖五、HPLC分析蘋果多酚中之成份 71
附圖六、Apple polyphenol主要組成物之化學結構式 72
拾壹、參考文獻 73

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