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研究生:林佩穎
研究生(外文):Pei-Ying
論文名稱:蘋果多酚延緩脂肪細胞分化並在實驗性肥胖中降低脂肪貯存
論文名稱(外文):Apple Polyphenols Decelerate Adipocyte Differentia-tion and Reduce Fat Storage in Experimental Obesity
指導教授:李彗禎
學位類別:碩士
校院名稱:中山醫學大學
系所名稱:生化暨生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
論文頁數:76
相關次數:
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肥胖已被認為具有造成糖尿病.心血管疾病及某些癌症等慢性疾病相關的高危險因子。先前的研究已知內臟中脂肪的堆積是造成代謝症候群的來源,如胰島素阻抗,與生活型態有關疾病如糖尿病、高脂血症及高血壓都會導致粥狀動脈硬化症的產生。於某些臨床報告中證實蘋果多酚具有調控脂質代謝。因此,於本研究中欲證實蘋果多酚於肥胖模式誘導下,其功效及機轉為何。細胞模式中,觀察蘋果多酚對於抑制3T3-L1細胞分化之影響。動物模式中,將倉鼠餵食高油脂飲食(含0.2%膽固醇及10%玉米油)誘導其肥胖,共為期八周,並觀察各組體重、肝臟重、腎臟周邊脂肪、性腺週邊脂肪、血清中三酸甘油酯、膽固醇、低密度脂蛋白及高密度脂蛋白。結果顯示,細胞模式中,蘋果多酚具有抑制3T3-L1細胞之分化能力。動物模式中,蘋果多酚可降低倉鼠體重及周邊脂肪重量。血清生化值結果中,蘋果多酚可降低血清中三酸甘油脂及膽固醇。以上的結果得知蘋果多酚具有降低脂質生成的能力。

The global risk of overweight/obesity as well as the high incidence of diabetes, cardiovascular disease, and specific cancers has been recognized to associate with chronic diseases. Previous studies showed that visceral fat accumulation induces metabolic syndrome associated with insulin resistance, causing lifestyle-related diseases such as diabetes, hyperlipidemia, and hypertension, leading to arteriosclerosis. In some clinical studies reveals apple polyphenols (AP) can regulate fat metabolism in healthy subjects with high body mass index. In this study, we tried to clarify the effects and mechanisms of AP in an experimental obesity model. In in vitro study, the effect of AP inhibiting 3T3-L1 differentiation was investigated. In in vivo study, a high fat diet (0.2% of cholesterol and 10 % of corn oil) was fed to hamsters for 8 weeks to induce obesity. The results showed the AP reduced the level of 3T3-L1 cell differentiating to adipocyte. AP also reduced the body weight, pararenal and gonadal fat, the levels of triglyceride and total cholesterol in obese hamsters. . Our results show that AP possesses the ability to reduce the adipogenesis.

中文摘要 V
英文摘要 VI
壹、縮寫表 1
貳、序論 2
2.1肥胖定義 2
2.2脂肪組織與脂肪細胞 2
2.3脂肪細胞分化 3
2.4脂肪細胞分化研究模式 7
2.5分化調控因子 7
2.6脂質調節 10
2.7蘋果多酚 11
2.8多酚與脂肪細胞分化相關研究 12
叁、研究動機 14
肆、實驗架構 15
伍、實驗儀器 16
陸、實驗藥品 18
柒、實驗方法 20
7.1動物實驗 20
7.2細胞實驗 29
捌、藥品配製: 34
玖、結果 41
一、動物實驗結果 41
二、細胞實驗結果 44
拾、討論 48
附圖一、肥胖定義 67
附圖二、世界衛生組織對於肥胖之分類 68
附圖三、in vitro model of adipocyte differentiation 69
附圖四、3T3-L1 細胞分化流程表 70
附圖五、HPLC分析蘋果多酚中之成份 71
附圖六、Apple polyphenol主要組成物之化學結構式 72
拾壹、參考文獻 73

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