(3.236.222.124) 您好!臺灣時間:2021/05/13 01:08
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:黃妤婕
研究生(外文):Yu-Jie
論文名稱:開發連線固相萃取-液相層析串聯質譜儀搭配2,4-二硝基苯胼衍生法分析尿液中丙二醛
論文名稱(外文):Urinary analysis of malondialdehyde by LC-MS/MS with automated solid-phase extraction following derivatization with 2,4-dinitrophenylhydrazine
指導教授:趙木榮趙木榮引用關係
指導教授(外文):Mu-Rong Chao
學位類別:碩士
校院名稱:中山醫學大學
系所名稱:醫學分子毒理學研究所
學門:醫藥衛生學門
學類:其他醫藥衛生學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
論文頁數:73
相關次數:
  • 被引用被引用:0
  • 點閱點閱:211
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
本研究成功建立一連線固相萃取-液相層析串聯質譜儀 (on-line SPE LC-MS/MS),搭配2,4-二硝基苯胼 (2,4-dinitrophenylhydrazine, DNPH) 衍生法,以快速準確的分析尿液中丙二醛 (malondialdehyde, MDA)。本研究的分析方法,尿液稀釋後與 DNPH 進行衍生反應,即可直接上機分析,不需再搭配其他前處理,層析時間為11分鐘。我們也利用建立的分析方法,探討 MDA 與 DNPH 衍生反應之最佳條件;發現尿液樣本中的 MDA 衍生反應要到達飽和所需添加的 DNPH 濃度約比 MDA 標準品高出60倍。此結果顯示尿液中富含其他與 DNPH 進行反應的物質,因此反應時需比標準品添加更多量的 DNPH,才足以將尿液中的 MDA 反應完全。接著我們將此方法應用於109個健康成年人的尿液樣本分析,其包含63個非吸菸者以及46個吸菸者,分析結果發現非抽菸者以及吸菸者尿液中的 MDA 平均值,分別為1.6 ± 0.9 μmol/g creatinine 以及 1.8 ± 1.8 μmol/g creatinine。兩個組別尿液中的 MDA 含量並無顯著的差異 (p > 0.05)。整體來說,本研究建立之分析方法快速準確,未來可運用於臨床高通量的樣本檢測,以評估人體內之氧化壓力。
此外,本研究也另將尿液樣本於衍生化前添加同位素內標 d2-MDA 並以 「on-line SPE LC-MS/MS」 進行分析,同時與不添加同位素內標之分析方法進行比較。其結果顯示與不添加內標的分析方法所測得的值,十分相近 (Pearson 相關係數 r = 0.9957, p < 0.001, n = 34)。

A high-throughput liquid chromatography/tandem mass spectrometry (LC/MS/MS) method coupled with an on-line solid phase extraction (SPE) system was developed to determine malondialdehyde (MDA) in urine following derivatization with 2,4-dinitrophenylhydrazine (DNPH). After derivatization, the urine samples can be directly analyzed within 11 min without further purification. The method was then applied to investigate the optimal reaction conditions for MDA derivatization using DNPH. The results showed that for a completely MDA derivatization, the additions of DNPH to MDA calibration solutions or urinary samples were extremely different. The optimal addition ratio of DNPH to urinary MDA is 60 times higher than that of MDA standard solution. This finding indicated that there were considerable interfering substances present in urine matrix that could also react with DNPH.
Furthermore, this “on-line SPE LC-MS/MS” method was applied to determine the urinary levels of MDA in 109 healthy adults, including 63 non-smokers and 46 smokers. The mean urinary MDA levels were 1.6 ± 0.9 and 1.8 ± 1.8 μmol/g creatinine for non-smokers and smokers,respectively. There was no significant difference in urinary MDA levels between the two groups (p > 0.05). Overall, this newly developed “on-line SPE LC-MS/MS” method could be applicable in both research and clinical practice for assessing oxidative stress.
In addition to this, the urine samples were also added with a stable isotope d2-MDA prior to derivatization and analyzed by on-line SPE LC-MS/MS. The results showed that the urinary MDA values obtained from both methods (with or without stable isotope) were highly correlated (Pearson correlation coefficient r = 0.9957, p < 0.001, n = 34).

摘要-I
Abstract-III
目錄-V
表目錄-VIII
圖目錄-IX
第一章 緒論
1-1 前言-1
1-2 研究動機-2
1-3 研究目標-3
第二章 文獻回顧
2-1 氧化壓力 (oxidative stress) 與脂質過氧化 (lipid peroxidation, LPO)-4
2-1-1 氧化壓力-4
2-1-2 脂質過氧化 (lipid peroxidation, LPO) 及其主要產物丙二醛 (malondialdehyde, MDA)-8
2-2 MDA 與疾病的相關性-14
2-3 MDA 的分析方法-19
第三章 材料與方法
3-1 化學藥品及試劑-25
3-2 人體尿液樣本-26
3-3 連線固相萃取-液相層析-串聯質譜儀分析方法開發-27
3-3-1 自動連線式固相萃取裝置 (automated on-line SPE)-27
3-3-2 液相層析系統 (LC)-30
3-3-3 電灑離子化串聯質譜儀 (electrospray ionization MS/MS)-30
3-4 MDA-DNPH 衍生反應最佳化-32
3-4-1 MDA 標準品與 DNPH 反應之反應曲線-32
3-4-2 尿液樣本與 DNPH 之反應曲線-33
3-5 分析方法確認-33
3-5-1 偵測極限 (limit of detection, LOD) 和定量極限 (limit of quantification, LOQ)-33
3-5-2 方法回收率 (recovery)、組內變異 (intra-day variation)-33
3-6 以 on-line SPE LC-MS/MS 分析尿液樣本-34
3-7 以 on-line SPE LC-MS/MS 搭配同位素內標稀釋法 (isotope dilution) 分析尿液樣本-35
3-8 統計分析-36
第四章、結果與討論
4-1 待測物 MDA-DNPH 的初步鑑定-37
4-2 on-line SPE LC-MS/MS 分析方法的建立-42
4-3 MDA 與 DNPH 衍生反應最佳化-44
4-3-1 MDA 標準品與 DNPH 之反應曲線-44
4-3-2 尿液樣本與 DNPH 之反應曲線-45
4-4 方法確認-47
4-4-1 偵測極限 (LOD) 和定量極限 (LOQ)-47
4-4-2 精準度 (precision) 和回收率 (recovery)-47
4-4-3 離子抑制 (ion suppression)-49
4-5 應用 on-line SPE LC-MS/MS 於一般健康成人之尿液 MDA 分析-52
4-6 on-line SPE-LC-MS/MS 搭配同位素稀釋法 (isotope dilution)-57
第五章 結論63
參考文獻-64

表目錄
表2-1 生物體 ROS 的來源-6
表2-2 MDA 與疾病之相關性-15
表2-3 各種層析方法於體液 MDA 之分析-22
表3-1 分析 MDA-DNPH 之 on-line SPE LC 管柱切換流程表-29
表3-2 待測物 MDA-DNPH 離子對選擇及其碰撞能量-32
表4-1 MDA-DNPH 以及 d2-MDA-DNPH 之離子對-41
表4-2 方法精準度與回收率-48
表4-3 分析方法比較 -50
表4-4 非吸菸者與吸菸者尿液中 MDA 的含量-53
表4-5 本研究及文獻中健康成年人尿液 MDA 的含量-54

圖目錄
圖2-1 ROS 與抗氧化系統之氧化壓力平衡圖-8
圖2-2 多元不飽和脂肪酸的脂質過氧化反應機制-10
圖2-3 常見的 MDA-DNA 共價鍵結產物-12
圖2-4 MDA 與蛋白質所形成之 Schiff base-13
圖2-5 MDA-TBA 衍生化物之形成機制-20
圖2-6 MDA-DNPH 衍生化物之形成機制-20
圖3-1 on-line SPE LC-MS/MS 之自動系統架構圖-28
圖4-1 待測物 MDA-DNPH 全離子掃描圖 (Q1 scan) 及結構圖-38
圖4-2 d2-MDA-DNPH 全離子掃描圖 (Q1 scan) 及結構圖-39
圖4-3 MDA-DNPH 子離子掃描圖 (product ion scan)-40
圖4-4 d2-MDA-DNPH 子離子掃描圖 (product ion scan)-41
圖4-5 MDA-DNPH 標準品之層析圖-43
圖4-6 尿液樣本 MDA-DNPH 之層析圖-44
圖4-7 0.2 mM MDA 與不同濃度的 DNPH 反應之反應曲線-45
圖4-8 尿液稀釋5倍與不同濃度的 DNPH 反應之反應曲線(n=3)-46
圖4-9 MDA-DNPH 方法回收率-48
圖4-10同位素內標添加前後之分析結果比較 (n=34)-58
圖4-11 僅含0.5 mM d2-MDA 內標準品之貢獻情形-60
圖4-12 僅含0.5 mM MDA 外標準品之貢獻情形-61



Aboutwerat, A., P. W. Pemberton, A. Smith, P. C. Burrows, R. F. McMahon, S. K. Jain and T. W. Warnes (2003). "Oxidant stress is a significant feature of primary biliary cirrhosis." Biochim Biophys Acta 1637(2): 142-150.
Agarwal, R. and S. D. Chase (2002). "Rapid, fluorimetric-liquid chromatographic determination of malondialdehyde in biological samples." J Chromatogr B Analyt Technol Biomed Life Sci 775(1): 121-126.
Aikens, J. and T. A. Dix (1991). "Perhydroxyl radical (HOO.) initiated lipid peroxidation. The role of fatty acid hydroperoxides." J Biol Chem 266(23): 15091-15098.
Akbulut, H., K. G. Akbulut, F. Icli and A. Buyukcelik (2003). "Daily variations of plasma malondialdehyde levels in patients with early breast cancer." Cancer Detect Prev 27(2): 122-126.
Alzoghaibi, M. A., I. A. Al Mofleh and A. M. Al-Jebreen (2007). "Lipid peroxides in patients with inflammatory bowel disease." Saudi J Gastroenterol 13(4): 187-190.
Andreoli, R., P. Manini, M. Corradi, A. Mutti and W. M. Niessen (2003). "Determination of patterns of biologically relevant aldehydes in exhaled breath condensate of healthy subjects by liquid chromatography/atmospheric chemical ionization tandem mass spectrometry." Rapid Commun Mass Spectrom 17(7): 637-645.
Arstila, A. U., M. A. Smith and B. F. Trump (1972). "Microsomal lipid peroxidation: morphological characterization." Science 175(21): 530-533.
Bakan, E., S. Taysi, M. F. Polat, S. Dalga, Z. Umudum, N. Bakan and M. Gumus (2002). "Nitric oxide levels and lipid peroxidation in plasma of patients with gastric cancer." Jpn J Clin Oncol 32(5): 162-166.
Basu, S. and M. Eriksson (1998). "Oxidative injury and survival during endotoxemia." FEBS Lett 438(3): 159-160.
Benamira, M., K. Johnson, A. Chaudhary, K. Bruner, C. Tibbetts and L. J. Marnett (1995). "Induction of mutations by replication of malondialdehyde-modified M13 DNA in Escherichia coli: determination of the extent of DNA modification, genetic requirements for mutagenesis, and types of mutations induced." Carcinogenesis 16(1): 93-99.
Bermejo, P., P. Gomez-Serranillos, J. Santos, E. Pastor, P. Gil and S. Martin-Aragon (1997). "Determination of malonaldehyde in Alzheimer''s disease: a comparative study of high-performance liquid chromatography and thiobarbituric acid test." Gerontology 43(4): 218-222.
Bonn, D. (1998). "How DNA-repair pathways may affect cancer risk." Lancet 351(9095): 42.
Breen, A. P. and J. A. Murphy (1995). "Reactions of oxyl radicals with DNA." Free Radic Biol Med 18(6): 1033-1077.
Carbonneau, M. A., E. Peuchant, D. Sess, P. Canioni and M. Clerc (1991). "Free and bound malondialdehyde measured as thiobarbituric acid adduct by HPLC in serum and plasma." Clin Chem 37(8): 1423-1429.
Castro, L. and B. A. Freeman (2001). "Reactive oxygen species in human health and disease." Nutrition 17(2): 161, 163-165.
Chao, M. R., C. J. Wang, L. W. Chang and C. W. Hu (2006). "Quantitative determination of urinary N7-ethylguanine in smokers and non-smokers using an isotope dilution liquid chromatography/tandem mass spectrometry with on-line analyte enrichment." Carcinogenesis 27(1): 146-151.
Chirico, S. (1994). "High-performance liquid chromatography-based thiobarbituric acid tests." Methods Enzymol 233: 314-318.
Cighetti, G., S. Debiasi, R. Paroni and P. Allevi (1999). "Free and total malondialdehyde assessment in biological matrices by gas chromatography-mass spectrometry: what is needed for an accurate detection." Anal Biochem 266(2): 222-229.
Cighetti, G., L. Duca, L. Bortone, S. Sala, I. Nava, G. Fiorelli and M. D. Cappellini (2002). "Oxidative status and malondialdehyde in beta-thalassaemia patients." Eur J Clin Invest 32 Suppl 1: 55-60.
Cordis, G. A., D. K. Das and W. Riedel (1998). "High-performance liquid chromatographic peak identification of 2,4-dinitrophenylhydrazine derivatives of lipid peroxidation aldehydes by photodiode array detection." J Chromatogr A 798(1-2): 117-123.
Cordis, G. A., N. Maulik and D. K. Das (1995). "Detection of oxidative stress in heart by estimating the dinitrophenylhydrazine derivative of malonaldehyde." J Mol Cell Cardiol 27(8): 1645-1653.
Corradi, M., I. Rubinstein, R. Andreoli, P. Manini, A. Caglieri, D. Poli, R. Alinovi and A. Mutti (2003). "Aldehydes in exhaled breath condensate of patients with chronic obstructive pulmonary disease." Am J Respir Crit Care Med 167(10): 1380-1386.
De Vecchi, A. F., F. Bamonti, C. Novembrino, S. Ippolito, L. Guerra, S. Lonati, S. Salini, C. S. Aman, E. Scurati-Manzoni and G. Cighetti (2009). "Free and total plasma malondialdehyde in chronic renal insufficiency and in dialysis patients." Nephrol Dial Transplant 24(8): 2524-2529.
Deighton, N., W. J. Magill, D. H. Bremner and E. E. Benson (1997). "Malondialdehyde and 4-hydroxy-2-nonenal in plant tissue cultures: LC-MS determination of 2,4-dinitrophenylhydrazone derivatives." Free Radic Res 27(3): 255-265.
Diaz-Velez, C. R., S. Garcia-Castineiras, E. Mendoza-Ramos and E. Hernandez-Lopez (1996). "Increased malondialdehyde in peripheral blood of patients with congestive heart failure." Am Heart J 131(1): 146-152.
Dib, M., C. Garrel, A. Favier, V. Robin and C. Desnuelle (2002). "Can malondialdehyde be used as a biological marker of progression in neurodegenerative disease?" J Neurol 249(4): 367-374.
DiCiero Miranda, M., V. M. de Bruin, M. R. Vale and G. S. Viana (2000). "Lipid peroxidation and nitrite plus nitrate levels in brain tissue from patients with Alzheimer''s disease." Gerontology 46(4): 179-184.
Draper, H. H., E. J. Squires, H. Mahmoodi, J. Wu, S. Agarwal and M. Hadley (1993). "A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials." Free Radic Biol Med 15(4): 353-363.
Dreissigacker, U., M. T. Suchy, N. Maassen and D. Tsikas (2010). "Human plasma concentrations of malondialdehyde (MDA) and the F2-isoprostane 15(S)-8-iso-PGF(2alpha) may be markedly compromised by hemolysis: evidence by GC-MS/MS and potential analytical and biological ramifications." Clin Biochem 43(1-2): 159-167.
Fenaille, F., P. Mottier, R. J. Turesky, S. Ali and P. A. Guy (2001). "Comparison of analytical techniques to quantify malondialdehyde in milk powders." J Chromatogr A 921(2): 237-245.
Fink, S. P., G. R. Reddy and L. J. Marnett (1997). "Mutagenicity in Escherichia coli of the major DNA adduct derived from the endogenous mutagen malondialdehyde." Proc Natl Acad Sci U S A 94(16): 8652-8657.
Frostegard, J., A. Haegerstrand, M. Gidlund and J. Nilsson (1991). "Biologically modified LDL increases the adhesive properties of endothelial cells." Atherosclerosis 90(2-3): 119-126.
Frostegard, J., J. Nilsson, A. Haegerstrand, A. Hamsten, H. Wigzell and M. Gidlund (1990). "Oxidized low density lipoprotein induces differentiation and adhesion of human monocytes and the monocytic cell line U937." Proc Natl Acad Sci U S A 87(3): 904-908.
Garrido, N., M. Meseguer, C. Simon, A. Pellicer and J. Remohi (2004). "Pro-oxidative and anti-oxidative imbalance in human semen and its relation with male fertility." Asian J Androl 6(1): 59-65.
Goldfarb, T. L., M. E. Jarvik and S. D. Glick (1970). "Cigarette nicotine content as a determinant of human smoking behavior." Psychopharmacologia 17(1): 89-93.
Gonenc, A., Y. Ozkan, M. Torun and B. Simsek (2001). "Plasma malondialdehyde (MDA) levels in breast and lung cancer patients." J Clin Pharm Ther 26(2): 141-144.
Greilberger, J., C. Koidl, M. Greilberger, M. Lamprecht, K. Schroecksnadel, F. Leblhuber, D. Fuchs and K. Oettl (2008). "Malondialdehyde, carbonyl proteins and albumin-disulphide as useful oxidative markers in mild cognitive impairment and Alzheimer''s disease." Free Radic Res 42(7): 633-638.
Guerra, L. N., C. Rios de Molina Mdel, E. A. Miler, S. Moiguer, M. Karner and J. A. Burdman (2005). "Antioxidants and methimazole in the treatment of Graves'' disease: effect on urinary malondialdehyde levels." Clin Chim Acta 352(1-2): 115-120.
Guillen-Sans, R. and M. Guzman-Chozas (1998). "The thiobarbituric acid (TBA) reaction in foods: a review." Crit Rev Food Sci Nutr 38(4): 315-330.
Hagen, U. (1989). "Biochemical aspects of radiation biology." Experientia 45(1): 7-12.
Hagfors, L., P. Leanderson, L. Skoldstam, J. Andersson and G. Johansson (2003). "Antioxidant intake, plasma antioxidants and oxidative stress in a randomized, controlled, parallel, Mediterranean dietary intervention study on patients with rheumatoid arthritis." Nutr J 2: 5.
Hasenfratz, M., B. Baldinger and K. Battig (1993). "Nicotine or tar titration in cigarette smoking behavior?" Psychopharmacology (Berl) 112(2-3): 253-258.
Hirano, T. (2008). "Repair system of 7, 8-dihydro-8-oxoguanine as a defense line against carcinogenesis." J Radiat Res (Tokyo) 49(4): 329-340.
Hu, C. W., M. R. Chao and C. H. Sie (2010). "Urinary analysis of 8-oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2''-deoxyguanosine by isotope-dilution LC-MS/MS with automated solid-phase extraction: Study of 8-oxo-7,8-dihydroguanine stability." Free Radic Biol Med 48(1): 89-97.
Hwang, E. S. and G. H. Kim (2007). "Biomarkers for oxidative stress status of DNA, lipids, and proteins in vitro and in vivo cancer research." Toxicology 229(1-2): 1-10.
Ilic, T. V., M. Jovanovic, A. Jovicic and M. Tomovic (1999). "Oxidative stress indicators are elevated in de novo Parkinson''s disease patients." Funct Neurol 14(3): 141-147.
Jacob, R. A., G. M. Aiello, C. B. Stephensen, J. B. Blumberg, P. E. Milbury, L. M. Wallock and B. N. Ames (2003). "Moderate antioxidant supplementation has no effect on biomarkers of oxidant damage in healthy men with low fruit and vegetable intakes." J Nutr 133(3): 740-743.
Jareno, E. J., F. Bosch-Morell, R. Fernandez-Delgado, J. Donat and F. J. Romero (1998). "Serum malondialdehyde in HIV seropositive children." Free Radic Biol Med 24(3): 503-506.
Kadiiska, M. B., B. C. Gladen, D. D. Baird, D. Germolec, L. B. Graham, C. E. Parker, A. Nyska, J. T. Wachsman, B. N. Ames, S. Basu, N. Brot, G. A. Fitzgerald, R. A. Floyd, M. George, J. W. Heinecke, G. E. Hatch, K. Hensley, J. A. Lawson, L. J. Marnett, J. D. Morrow, D. M. Murray, J. Plastaras, L. J. Roberts, 2nd, J. Rokach, M. K. Shigenaga, R. S. Sohal, J. Sun, R. R. Tice, D. H. Van Thiel, D. Wellner, P. B. Walter, K. B. Tomer, R. P. Mason and J. C. Barrett (2005). "Biomarkers of oxidative stress study II: are oxidation products of lipids, proteins, and DNA markers of CCl4 poisoning?" Free Radic Biol Med 38(6): 698-710.
Kesavulu, M. M., B. K. Rao, R. Giri, J. Vijaya, G. Subramanyam and C. Apparao (2001). "Lipid peroxidation and antioxidant enzyme status in Type 2 diabetics with coronary heart disease." Diabetes Res Clin Pract 53(1): 33-39.
Khoschsorur, G. A., B. M. Winklhofer-Roob, H. Rabl, T. Auer, Z. Peng and R. J. Schaur (2000). "Evaluation of a sensitive HPLC method for the determination of malondialdehyde, and application of the method to different biological materials." Chromatographia 52(3-4): 181-184.
Klaunig, J. E. and L. M. Kamendulis (2004). "The role of oxidative stress in carcinogenesis." Annu Rev Pharmacol Toxicol 44: 239-267.
Klein, C. B., K. Frenkel and M. Costa (1991). "The role of oxidative processes in metal carcinogenesis." Chem Res Toxicol 4(6): 592-604.
Knight, J. A., R. K. Pieper and L. McClellan (1988). "Specificity of the thiobarbituric acid reaction: its use in studies of lipid peroxidation." Clin Chem 34(12): 2433-2438.
Koba, S., R. Pakala, T. Watanabe, T. Katagiri and C. R. Benedict (2000). "Synergistic interaction between thromboxane A2 and mildly oxidized low density lipoproteins on vascular smooth muscle cell proliferation." Prostaglandins Leukot Essent Fatty Acids 63(6): 329-335.
Kocak, H., Y. Oner-Iyidogan, F. Gurdol, T. Kocak and D. Esin (2005). "The relation between serum MDA and cystatin C levels in chronic spinal cord injury patients." Clin Biochem 38(11): 1034-1037.
Korchazhkina, O., C. Exley and S. Andrew Spencer (2003). "Measurement by reversed-phase high-performance liquid chromatography of malondialdehyde in normal human urine following derivatisation with 2,4-dinitrophenylhydrazine." J Chromatogr B Analyt Technol Biomed Life Sci 794(2): 353-362.
Korchazhkina, O., E. Jones, M. Czauderna and S. A. Spencer (2006). "Effects of exclusive formula or breast milk feeding on oxidative stress in healthy preterm infants." Arch Dis Child 91(4): 327-329.
Kugiyama, K., S. A. Kerns, J. D. Morrisett, R. Roberts and P. D. Henry (1990). "Impairment of endothelium-dependent arterial relaxation by lysolecithin in modified low-density lipoproteins." Nature 344(6262): 160-162.
Kumar, A., R. Mittal, H. D. Khanna and S. Basu (2008). "Free radical injury and blood-brain barrier permeability in hypoxic-ischemic encephalopathy." Pediatrics 122(3): e722-727.
Lee, B. R. and H. D. Um (1999). "Hydrogen peroxide suppresses U937 cell death by two different mechanisms depending on its concentration." Exp Cell Res 248(2): 430-438.
Lee, K. H., H. Bartsch, J. Nair, D. H. Yoo, Y. C. Hong, S. H. Cho and D. H. Kang (2006). "Effect of short-term fasting on urinary excretion of primary lipid peroxidation products and on markers of oxidative DNA damage in healthy women." Carcinogenesis 27(7): 1398-1403.
Lee, K. H. and D. Kang (2008). "Stability and intra-individual variation of urinary malondialdehyde and 2-naphthol." J Prev Med Public Health 41(3): 195-199.
Lee, K. H., X. O. Shu, Y. T. Gao, B. T. Ji, G. Yang, A. Blair, N. Rothman, W. Zheng, W. H. Chow and D. Kang (2010). "Breast cancer and urinary biomarkers of polycyclic aromatic hydrocarbon and oxidative stress in the Shanghai Women''s Health Study." Cancer Epidemiol Biomarkers Prev 19(3): 877-883.
Li, N., X. Jia, C. Y. Chen, J. B. Blumberg, Y. Song, W. Zhang, X. Zhang, G. Ma and J. Chen (2007). "Almond consumption reduces oxidative DNA damage and lipid peroxidation in male smokers." J Nutr 137(12): 2717-2722.
Lin, T. H., Y. L. Huang and W. C. Tseng (1995). "Arsenic and lipid peroxidation in patients with blackfoot disease." Bull Environ Contam Toxicol 54(4): 488-493.
Lovell, M. A., W. D. Ehmann, S. M. Butler and W. R. Markesbery (1995). "Elevated thiobarbituric acid-reactive substances and antioxidant enzyme activity in the brain in Alzheimer''s disease." Neurology 45(8): 1594-1601.
Lykkesfeldt, J., M. Viscovich and H. E. Poulsen (2004). "Plasma malondialdehyde is induced by smoking: a study with balanced antioxidant profiles." Br J Nutr 92(2): 203-206.
Marcus, D. L., C. Thomas, C. Rodriguez, K. Simberkoff, J. S. Tsai, J. A. Strafaci and M. L. Freedman (1998). "Increased peroxidation and reduced antioxidant enzyme activity in Alzheimer''s disease." Exp Neurol 150(1): 40-44.
Maridonneau, I., P. Braquet and R. P. Garay (1983). "Na+ and K+ transport damage induced by oxygen free radicals in human red cell membranes." J Biol Chem 258(5): 3107-3113.
Marnett, L. J. (1999). "Lipid peroxidation-DNA damage by malondialdehyde." Mutat Res 424(1-2): 83-95.
Mateos, R. and L. Bravo (2007). "Chromatographic and electrophoretic methods for the analysis of biomarkers of oxidative damage to macromolecules (DNA, lipids, and proteins)." J Sep Sci 30(2): 175-191.
Mateos, R., L. Goya and L. Bravo (2004). "Determination of malondialdehyde by liquid chromatography as the 2,4-dinitrophenylhydrazone derivative: a marker for oxidative stress in cell cultures of human hepatoma HepG2." J Chromatogr B Analyt Technol Biomed Life Sci 805(1): 33-39.
McIntosh, L. J., M. A. Trush and J. C. Troncoso (1997). "Increased susceptibility of Alzheimer''s disease temporal cortex to oxygen free radical-mediated processes." Free Radic Biol Med 23(2): 183-190.
Mercken, E. M., G. J. Hageman, A. M. Schols, M. A. Akkermans, A. Bast and E. F. Wouters (2005). "Rehabilitation decreases exercise-induced oxidative stress in chronic obstructive pulmonary disease." Am J Respir Crit Care Med 172(8): 994-1001.
Merendino, R. A., F. Salvo, A. Saija, G. Di Pasquale, A. Tomaino, P. L. Minciullo, G. Fraccica and S. Gangemi (2003). "Malondialdehyde in benign prostate hypertrophy: a useful marker?" Mediators Inflamm 12(2): 127-128.
Merry, P., M. Grootveld, J. Lunec and D. R. Blake (1991). "Oxidative damage to lipids within the inflamed human joint provides evidence of radical-mediated hypoxic-reperfusion injury." Am J Clin Nutr 53(1 Suppl): 362S-369S.
Nakai, K., K. Yoneda, R. Maeda, A. Munehiro, N. Fujita, I. Yokoi, J. Moriue, T. Moriue, H. Kosaka and Y. Kubota (2009). "Urinary biomarker of oxidative stress in patients with psoriasis vulgaris and atopic dermatitis." J Eur Acad Dermatol Venereol 23(12): 1405-1408.
Niedernhofer, L. J., J. S. Daniels, C. A. Rouzer, R. E. Greene and L. J. Marnett (2003). "Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cells." J Biol Chem 278(33): 31426-31433.
Niemela, O. (1999). "Aldehyde-protein adducts in the liver as a result of ethanol-induced oxidative stress." Front Biosci 4: D506-513.
Noberasco, G., P. Odetti, D. Boeri, M. Maiello and L. Adezati (1991). "Malondialdehyde (MDA) level in diabetic subjects. Relationship with blood glucose and glycosylated hemoglobin." Biomed Pharmacother 45(4-5): 193-196.
Oltra, A. M., F. Carbonell, C. Tormos, A. Iradi and G. T. Saez (2001). "Antioxidant enzyme activities and the production of MDA and 8-oxo-dG in chronic lymphocytic leukemia." Free Radic Biol Med 30(11): 1286-1292.
Oteiza, P. I., O. D. Uchitel, F. Carrasquedo, A. L. Dubrovski, J. C. Roma and C. G. Fraga (1997). "Evaluation of antioxidants, protein, and lipid oxidation products in blood from sporadic amyotrophic lateral sclerosis patients." Neurochem Res 22(4): 535-539.
Pan, C. H., C. C. Chan, Y. L. Huang and K. Y. Wu (2008). "Urinary 1-hydroxypyrene and malondialdehyde in male workers in Chinese restaurants." Occup Environ Med 65(11): 732-735.
Paradies, G., F. M. Ruggiero, G. Petrosillo and E. Quagliariello (1997). "Alterations in carnitine-acylcarnitine translocase activity and in phospholipid composition in heart mitochondria from hypothyroid rats." Biochim Biophys Acta 1362(2-3): 193-200.
Pilz, J., I. Meineke and C. H. Gleiter (2000). "Measurement of free and bound malondialdehyde in plasma by high-performance liquid chromatography as the 2,4-dinitrophenylhydrazine derivative." J Chromatogr B Biomed Sci Appl 742(2): 315-325.
Polidori, M. C., K. Savino, G. Alunni, M. Freddio, U. Senin, H. Sies, W. Stahl and P. Mecocci (2002). "Plasma lipophilic antioxidants and malondialdehyde in congestive heart failure patients: relationship to disease severity." Free Radic Biol Med 32(2): 148-152.
Rauli, S., M. D. Puppo, F. Magni and M. G. Kienle (1998). "Validation of malondialdehyde and 4-hydroxy-2-trans-nonenal measurement in plasma by NICI-GC-MS." J Biochem 123(5): 918-923.
Requena, J. R., M. X. Fu, M. U. Ahmed, A. J. Jenkins, T. J. Lyons, J. W. Baynes and S. R. Thorpe (1997). "Quantification of malondialdehyde and 4-hydroxynonenal adducts to lysine residues in native and oxidized human low-density lipoprotein." Biochem J 322 ( Pt 1): 317-325.
Romieu, I., A. Barraza-Villarreal, C. Escamilla-Nunez, A. C. Almstrand, D. Diaz-Sanchez, P. D. Sly and A. C. Olin (2008). "Exhaled breath malondialdehyde as a marker of effect of exposure to air pollution in children with asthma." J Allergy Clin Immunol 121(4): 903-909 e906.
Sheu, J. Y., H. P. Ku, W. C. Tseng, M. T. Chen, L. Y. Tsai and Y. L. Huang (2003). "Determination of thiobarbituric acid adduct of malondialdehyde using on-line microdialysis coupled with high-performance liquid chromatography." Anal Sci 19(4): 621-624.
Shin, H. S. and D. G. Jung (2009). "Sensitive Analysis of Malondialdehyde in Human Urine by Derivatization with Pentafluorophenylhydrazine then Headspace GC-MS." Chromatographia 70(5-6): 899-903.
Sim, A. S., C. Salonikas, D. Naidoo and D. E. Wilcken (2003). "Improved method for plasma malondialdehyde measurement by high-performance liquid chromatography using methyl malondialdehyde as an internal standard." J Chromatogr B Analyt Technol Biomed Life Sci 785(2): 337-344.
Stalikas, C. D. and C. N. Konidari (2001). "Analysis of malondialdehyde in biological matrices by capillary gas chromatography with electron-capture detection and mass spectrometry." Anal Biochem 290(1): 108-115.
Stokvis, E., H. Rosing and J. H. Beijnen (2005). "Stable isotopically labeled internal standards in quantitative bioanalysis using liquid chromatography/mass spectrometry: necessity or not?" Rapid Commun Mass Spectrom 19(3): 401-407.
Suleyman, H., K. Gumustekin, S. Taysi, S. Keles, N. Oztasan, O. Aktas, K. Altinkaynak, H. Timur, F. Akcay, S. Akar, S. Dane and M. Gul (2002). "Beneficial effects of Hippophae rhamnoides L. on nicotine induced oxidative stress in rat blood compared with vitamin E." Biological & Pharmaceutical Bulletin 25(9): 1133-1136.
Templar, J., S. P. Kon, T. P. Milligan, D. J. Newman and M. J. Raftery (1999). "Increased plasma malondialdehyde levels in glomerular disease as determined by a fully validated HPLC method." Nephrol Dial Transplant 14(4): 946-951.
Uchendu, E. E., S. W. Leonard, M. G. Traber and B. M. Reed (2010). "Vitamins C and E improve regrowth and reduce lipid peroxidation of blackberry shoot tips following cryopreservation." Plant Cell Rep 29(1): 25-35.
Verhaegh, G. W., M. J. Richard and P. Hainaut (1997). "Regulation of p53 by metal ions and by antioxidants: dithiocarbamate down-regulates p53 DNA-binding activity by increasing the intracellular level of copper." Mol Cell Biol 17(10): 5699-5706.
Wang, J. P., R. Maddalena, B. Zheng, C. Zai, F. Liu and J. C. Ng (2009). "Arsenicosis status and urinary malondialdehyde (MDA) in people exposed to arsenic contaminated-coal in China." Environ Int 35(3): 502-506.
Waris, G. and H. Ahsan (2006). "Reactive oxygen species: role in the development of cancer and various chronic conditions." J Carcinog 5: 14.
Weinbrenner, T., M. Fito, R. de la Torre, G. T. Saez, P. Rijken, C. Tormos, S. Coolen, M. F. Albaladejo, S. Abanades, H. Schroder, J. Marrugat and M. I. Covas (2004). "Olive oils high in phenolic compounds modulate oxidative/antioxidative status in men." J Nutr 134(9): 2314-2321.
Wu, L. L., C. C. Chiou, P. Y. Chang and J. T. Wu (2004). "Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics." Clin Chim Acta 339(1-2): 1-9.
Yagi, K. (1976). "A simple fluorometric assay for lipoperoxide in blood plasma." Biochem Med 15(2): 212-216.
Yang, Y. D. (1998). "Simultaneous determination of creatine, uric acid, creatinine and hippuric acid in urine by high performance liquid chromatography." Biomed Chromatogr 12(2): 47-49.
Yeo, H. C., H. J. Helbock, D. W. Chyu and B. N. Ames (1994). "Assay of malondialdehyde in biological fluids by gas chromatography-mass spectrometry." Anal Biochem 220(2): 391-396.




QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關論文
 
系統版面圖檔 系統版面圖檔