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研究生:曾祐德
研究生(外文):Yu-Te Tzeng
論文名稱:五味子和芝麻萃取混合物抗氧化及護肝作用之研究
論文名稱(外文):Study of the mixture of schisandra and sesame on antioxidation and liver protection
指導教授:王進崑王進崑引用關係陳肅霖陳肅霖引用關係
指導教授(外文):Chin-Kun Wang王進崑Su-Lin Chen陳肅霖
學位類別:碩士
校院名稱:中山醫學大學
系所名稱:營養學系碩士班
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
論文頁數:94
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五味子及芝麻被發現對於四氯化碳(CCl4)所誘發肝損傷之老鼠具有保護的作用。本研究擬探討五味子與芝麻混合物之抗氧化力及抗發炎的效果,另外以人體試驗探討護肝之功能,並測定對人體抗氧化能力與抗氧化酵素活性之影響。
人體試驗共計40位肝指數偏高(GOT或GPT介於40~100U/L)之健康受試者全程參與,採隨機、雙盲、安慰劑組對照進行。本試驗將受試者分為兩組,一組服用五味子與芝麻之混合物,另一組則服用安慰劑,試驗前的一個月為run in period,試驗期為五個月,試驗期後的一個月為follow up period。受試者每天服用兩次的試驗樣品或安慰劑,每次服用兩錠,一次為午餐飯前,一次則為晚餐飯後。試驗前後進行體位測量,每個月採集血液檢體,並於試驗之起始,及第三、五個月進行腹部超音波檢測。
結果顯示,連續服用五味子及芝麻混合物五個月後,可以顯著降低血漿glutamate oxaloacetate transaminase(GOT)及glutamate
pyruvate transaminase(GPT),延長血漿中低密度脂蛋白(low
density lipoprotein;LDL)氧化延遲時間,提昇受試者體內總抗氧化能力和降低脂質過氧化反應,並顯著提高紅血球中glutathione
peroxidase(GSH Px)及glutathione reductase(GSH Rd)之抗氧化酵素活性。

Schisandra and sesame were found protective from carbon tetrachloride (CCl4) induced liver damage on rats. This study was focused on the anti-oxidant capacity and anti-inflammatory effect of the mixture of schisandra and sesame. A human clinical trial was used for its protection from liver inflammation, and also determined the antioxidant capacity and enzyme activities.
Forty healthy subjects were completed involved in this study, whose liver function index were slight high (GOT or GPT in the range of 40 to 100 U/L). This was a randomized, placebo controlled and double blind design. We randomized subjects into two groups. One group of subjects took the mixture of schisandra and sesame tablet, the another group took placebo. One month before administration was run in period. Experimental period was five months, one month after the stop of administration was follow-up period. Each subject took the mixture of schisandra and sesame tablet or placebo twice a day. Two tablets were taken before lunch, and the other two were after dinner. Anthropometric measurements at initial and end of study were determined. Blood collection every month, and abdominal ultrasounic examination were performed at the initial, 3rd and 5th month.
Five months of continuous administration of the mixture of schisandra and sesame tablet could significantly lower GOT and GPT and prolonging the lag time of LDL oxidation. In addition the total antioxidatant activity, GSH Px and GSH Rd were greatly increased.

表次 I
圖次 II
附錄 III
中文摘要 IV
英文摘要 V
壹、 前言 1
貳、 文獻整理
一、 五味子之組成分及護肝功效
(一) 五味子 2
(二) 五味子之護肝功效
1. 肝功能指數 4
2. 動物體內抗氧化功能 8
3. 抗發炎功能 8
二、 芝麻之組成與功效
(一) 芝麻 11
(二) 芝麻之功效
1. 調整脂質代謝 11
2. 抗發炎 14
3. 抗細胞凋亡方面 14
參、 研究目的 16
肆、 實驗設計 17
伍、 材料與方法
一、 試驗材料
(一) 五味子與芝麻混合物 18
(二) 人體試驗
1. 基本資料收集 19
2. 血液樣品收集 19
二、 試驗用藥 21
三、 試驗儀器 24
四、 試驗方法
(一) 五味子芝麻錠萃取物製備 26
(二) 抗氧化性測定
1. 總抗氧化能力 26
2. 還原力 26
3. 螯合亞鐵能力 27
4. 清除活性氧能力
a. DPPH 自由基清除能力 27
b. H2O2 清除能力 27
c. 超氧陰離子清除能力 28
(三) 人體臨床試驗
1. 血液樣本之收集 28
2. 肝功能評估及腹部超音波檢測
a. 肝功能評估 28
b. 腹部超音波檢測 29
3. 血漿抗氧化活性測定
a. 血漿中總抗氧化狀態 29
b. 血漿中脂質過氧化物含量(TBARS) 29
c. 血漿中 LDL 氧化延滯期 30
4. 抗氧化酵素活性分析
a. GSH Px 31
b. GSH Rd 32
5.自由基測定
a. 全血總自由基含量 33
b. 全血超氧陰離子含量 34
五、 統計分析 36

陸、 結果與討論
一、抗氧化性測定
(一) 總抗氧化能力 37
(二) 還原力 37
(三) 螯合亞鐵能力 39
(四) 清除活性氧能力
1. DPPH 自由基清除能力 39
2. H2O2 清除能力 42
3. 超氧陰離子清除能力 42
二、人體臨床試驗
(一) 受測者特性 48
(二) 護肝功能之評估 48
(三) 受試者 LDL 氧化延滯之影響 57
(四) 全血自由基之影響 57
(五) 對血漿抗氧化功能之影響 59
(六) 對紅血球抗氧化酵素活性之影響 65
柒、 結論 71
捌、 參考文獻 72
玖、 附錄 78


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