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研究生:林嘉祥
研究生(外文):Jia-Siang
論文名稱:鳥胺酸去羧化酶降低二甲亞碸所誘導類嗜中性白血球分化、活化以及細胞凋亡
論文名稱(外文):Ornithine decarboxylase attenuates dimethyl sulfoxide-induced neutrophil-like differentiation, activation and apoptosis
指導教授:林志立
指導教授(外文):Chih-Li Lin
學位類別:碩士
校院名稱:中山醫學大學
系所名稱:醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
論文頁數:60
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鳥胺酸去羧化酶(ornithine decarboxylase; ODC)為多胺酸(polyamine)合成路徑的反應速率決定步驟,將鳥胺酸(L-ornithine)去羧化成腐胺(putrescine)以及二氧化碳進而生成亞精胺(spermidine)以及精胺(spermine),鳥胺酸去羧化酶在生物功能上也扮演著重要的角色,對於細胞的生長、發育、細胞凋亡以及細胞的分化等等生物功能,先前的研究都指出鳥胺酸去羧化酶會參與其中,過量的鳥胺酸去羧化酶與多胺類會抑制巨噬細胞的免疫反應,以及影響骨髓性白血球細胞的分化。本篇探討骨髓性白血球細胞的分化成嗜中性白血球時是否因為鳥胺酸去羧化酶的過量表現而影響了類嗜中性白血球的分化、活化以及死亡。首先我們使用dimethyl sulfoxide(DMSO)促進細胞分化,觀察到HL-60細胞存活率沒有因為加入DMSO而下降,接著利用劉氏染色、吞噬作用以及nitroblue tetrazolium(NBT)觀察到分化成類嗜中性白血球後細胞的形態和功能受到鳥胺酸去羧化酶的過度表現而受到了抑制。死亡方面,細胞凋亡的特徵像是凋亡小體的形成、染色質的片斷化以及細胞周期中SubG1 peak的形成都因為鳥胺酸去羧化酶得過量而延緩細胞的死亡。人類嗜中性白血球經由Fas訊息傳導路徑而走向死亡,在這裡我們也發現是鳥胺酸去羧化酶干擾Fas的表現而影響細胞的死亡,而Fas的下游蛋白caspase 8, t-bid, Bax, caspase 9, caspase 3的表現也因為鳥胺酸去羧化酶的表現增加有被抑制的現象,在這裡我們發現因為轉錄因子CCAAT/enhancer binding protein(C/EBP family protein)以及PU.1表現量下降而降低了細胞的分化和死亡。本篇探討鳥胺酸去羧化酶的過量表現影響了細胞的分化以及死亡而鳥胺酸去羧化酶可能是為其中一種干擾骨髓細胞分化而形成血癌的因子。

Ornithine decarboxylase (ODC), a tumor promoter, provokes cell proliferation, and inhibits cell death. A human leukemic cell line (designated HL-60) has been established from the peripheral blood leukocytes of a patient with acute promyelocytic leukemia. In past research we study ODC interferes with macrophage-like differentiation. Herein, we examine whether it functions during neutrophil-like differentiation. By using dimethyl-sulfoxide (DMSO) differentiated human promyelocytic HL-60, we discover cluster of differentiation molecule 11B (CD11B) is a granulocyte integrin as neutrophil differentiation marker and observe its nuclear morphology. Temporarily conventional expression of ODC represses polar compound-induced terminal myeloid differentiation and activation by Liu’s stain, phagocytosis and NBT assays. Furthermore, ODC overexpression reduced CD95/Fas and its transcription factor mRNA expression. Fas has known to induce neutrophil apoptosis. ODC-attenuated DMSO-induced cell death is through an extrinsic apoptosis pathway, includes Fas, caspase 8, t-Bid, Bax, caspase 9 and caspase 3 protein expression. Consequently we suggest that ODC inhibits DMSO-induced human neutrophil-like differentiation, activation and apoptotic cell death. These interrupting mechanisms may provide the possible reason of neutrophil immaturation and why leukemia is poor differentiation under abnormally not death.

目錄(Index)
封面....................................................1
目錄....................................................2
Ⅰ.Abstract.............................................6
Ⅱ.中文摘要.............................................7
Ⅲ.前言(Introduction).................................9
1. 鳥胺酸去羧化酶....................................9
2. 血癌(白血病)....................................12
3. 嗜中性白血球的分化及其特性........................13
4. 細胞凋亡..........................................18
5. 研究動機與目的....................................20
Ⅳ.材料與方法(Materials and Methods)..................21
1. 細胞培養..........................................21
2. 細胞存活率........................................21
3. 吖啶橙染色........................................21
4. 去核糖核酸片段化的檢測............................21
5. 流式細胞儀分析....................................22
6. 劉氏染色..........................................22
7. 硝基藍四氮唑分析..................................23
8. 吞噬作用..........................................23
9. 免疫螢光染色......................................24
10. Luciferase reporter分析...........................24
11. 反轉錄聚合酵素鏈鎖反應(RT-PCR)..................25
12. 免疫點墨法........................................26
13. 分離人類嗜中性白血球..............................27
14. 統計分析..........................................28
Ⅴ.結果(Results)......................................29
1. 鳥胺酸去羧化酶過量表現影響CD11b的RNA表現、細胞凋亡
的型態、Fas、C/EBPs 家族、PU.1的RNA表現、hfas promoter
的活性以反應及caspase 8表現(廖雅芳助理教授之研究)29
2. 鳥胺酸去羧化酶抑制類嗜中性白血球的分化............31
3. 鳥胺酸去羧化酶抑制類嗜中性白血球吞噬的活性以及氧爆作
用...................................................32
4. 鳥胺酸去羧化酶降低二甲基亞碸誘導細胞的死亡以及Fas蛋白
質的表現.............................................33
5. 鳥胺酸去羧化酶的過度表現抑制了t-Bid和Bax的表現,降低了caspase 9以及caspase 3的活性............................34
6. 腐胺的過度表現對於人類嗜中性白血球細胞的影響......35
Ⅵ.討論(Discussion)...................................36
Ⅶ.圖表(Figures)......................................41
圖一. A 膜蛋白CD11B核醣核酸表現......................41
圖一. B C/EBP以及PU.1核醣核酸表現....................41
圖一. C 去氧核醣核酸片斷化測試.......................42
圖一. D 流式細胞儀sub-G1的測定.......................42
圖一. E Fas核醣核酸表現..............................43
圖一. F hfas 啟動子 luciferase 活性測試..............43
圖一. G caspase 8 蛋白質表現.........................43
圖二. A 細胞核型態...................................45
圖二. B 類嗜中性白血球的分化計數.....................45
圖二. C 免疫螢光染色CD11b表現........................46
圖三. A 吞噬作用以及其計數...........................47
圖三. B 氧爆作用.....................................48
圖四. A 細胞存活率...................................49
圖四. B 凋亡小體免疫螢光染色.........................49
圖四. C 膜蛋白Fas免疫螢光表現…......................50
圖五. A t-Bid、BAX、caspase 9以及actin蛋白質表現.....51
圖五. B caspase 3蛋白質表現..........................51
圖六 總結............................................52
圖七 A 正常人類嗜中性白血球細胞型態..................53
圖七 B正常人類嗜中性白血球存活率與腐胺的關係.........53
Ⅷ. 參考文獻(References)..............................54


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