從1969年Ashkin發表光鉗(Optical Tweezers)的原理及方法以來，光鉗技術的研發已逐漸成熟。然而雷射光鉗技術雖然已被研發多年，也成功的用於生物細胞的操弄，但是在生物醫學檢測的應用仍有很大的發展空間；而且結合光鉗和微機械結構的生醫感測研究，在國際上仍屬少見。
因光鉗作用力受到雷射與所架設光路之影響，研究前需針對架設之光鉗進行作用力校正，故本研究針對各尺寸微珠測量作用力相對基準值，做為感測器開發時的基礎，並提出利用癱瘓精子與活潑精子作用力差值求得精子游動力之新感測方法，另一方面結合雙光子光致聚合微製造技術(TPP)提出製作槓桿生醫感測器之應用設計，做為測量細胞勁度的工具，開創結合光鉗和微機械結構在生醫檢測應用的可行性。
本研究針對各種尺寸的微珠測量出雷射功率與光鉗作用力的相對關係圖，此一關係可做為感測器中各種尺寸球體微元件結構雷射光鉗對其產生作用力之依據，而研究中可知細胞的折射率與吸收率為影響細胞作用力的兩大因素，另一方面提出使用微槓桿測量細胞勁度之新構想，能即時針對細胞勁度進行篩檢，在生物細胞作用力之相關研究上為生物細胞檢測奠定基礎。

Since 1969, Ashkin announced the methods and the principles of optical tweezers. The optical tweezers technology research and development has gradually matured. Despite, the laser optical tweezers technique has been researched, developed, and used successfully for biological cell manipulation for many years, there are still more improvement in the application of bio-medicine; moreover, the combination between optical tweezers and micro-mechanical sensor of bio-medical research is still rare seen between the countries.
The optical tweezers force was affected by laser and optical tweezers system. Therefore, it needs to be calibrated before you measure it. In the research, we measured force relative reference value by every size bead, and proposed a new sensing method, which used the loss of paralysis and active sperm trapping force to measure sperm mobility. Furthermore, proposed an application design combined two-photon absorption photo polymerization (TPP) to make lever biomedical sensors, which is a tool to measure cell stiffness, developing its feasibility of biomedical testing application on combination optical and TPP.
In the research, we focus on measured relative of laser power and trapping force plan by every size bead. This research can conduct laser tweezers force basis of any size micro particle element in the sensor. The study showed two main factors effect upon cell communication were the index of cell refraction and absorptive. Furthermore, proposed a new scheme used micro lever to measure cell stiffness, can sieve out and analyze cell stiffness in time. Thence laid the foundation of biological cells interaction research.

目錄
摘要 I
Abstract II
圖目錄 V
表目錄 VII
第一章 研究目的 1
1.1 前言 1
1.2 目的 2
1.3 方法 4
第二章 理論與研究背景介紹 7
2.1 雷射光鉗 7
2.1.1 光鉗原理 7
2.1.2 光鉗歷史 15
2.2 雙光子光致聚合微製造(TPP) 21
2.3 細胞介紹 28
2.3.1 精子 28
2.3.2 羊水細胞 31
2.3.3 癌化細胞與細胞勁度 34
2.4 槓桿 37
第三章 實驗方法 40
3.1 實驗架設 40
3.2 樣本製作 44
3.3 計算及測量方法 47
3.3.1 RO模型及EM模型理論值計算 47
3.3.2 脫離速度測量光鉗作用力 50
3.3.3 調制雷射功率測量精子游動力 53
第四章 結果與討論 54
4.1 理論值計算結果 54
4.2 微珠作用力測量結果 56
4.3 微珠作用力與細胞作用力結果分析 58
4.4 精子作用力測量結果 61
4.4.1 脫離速度測量精子游動力 62
4.4.2 調制雷射功率測量精子游動力 64
4.5生醫感測器應用之設計 66
第五章 結論與未來展望 75
5.1 結論 75
5.2 未來展望 76
參考文獻 78

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