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研究生:曾彥方
研究生(外文):Yen-fang Tseng
論文名稱:光鉗作用力在生物細胞力學感測上的應用開發
論文名稱(外文):Sensor Development of Induced Force by Optical Tweezers in Bio-Cell Mechanics Application
指導教授:林志郎林志郎引用關係
指導教授(外文):Chih-lang Lin
學位類別:碩士
校院名稱:中臺科技大學
系所名稱:醫學工程暨材料研究所
學門:工程學門
學類:綜合工程學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
論文頁數:97
中文關鍵詞:精子游動力細胞勁度雷射光鉗光鉗作用力微槓桿
外文關鍵詞:optical tweezerstweezers trapping forcemicro leversperm mobilitycell stiffness
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從1969年Ashkin發表光鉗(Optical Tweezers)的原理及方法以來,光鉗技術的研發已逐漸成熟。然而雷射光鉗技術雖然已被研發多年,也成功的用於生物細胞的操弄,但是在生物醫學檢測的應用仍有很大的發展空間;而且結合光鉗和微機械結構的生醫感測研究,在國際上仍屬少見。
因光鉗作用力受到雷射與所架設光路之影響,研究前需針對架設之光鉗進行作用力校正,故本研究針對各尺寸微珠測量作用力相對基準值,做為感測器開發時的基礎,並提出利用癱瘓精子與活潑精子作用力差值求得精子游動力之新感測方法,另一方面結合雙光子光致聚合微製造技術(TPP)提出製作槓桿生醫感測器之應用設計,做為測量細胞勁度的工具,開創結合光鉗和微機械結構在生醫檢測應用的可行性。
本研究針對各種尺寸的微珠測量出雷射功率與光鉗作用力的相對關係圖,此一關係可做為感測器中各種尺寸球體微元件結構雷射光鉗對其產生作用力之依據,而研究中可知細胞的折射率與吸收率為影響細胞作用力的兩大因素,另一方面提出使用微槓桿測量細胞勁度之新構想,能即時針對細胞勁度進行篩檢,在生物細胞作用力之相關研究上為生物細胞檢測奠定基礎。
Since 1969, Ashkin announced the methods and the principles of optical tweezers. The optical tweezers technology research and development has gradually matured. Despite, the laser optical tweezers technique has been researched, developed, and used successfully for biological cell manipulation for many years, there are still more improvement in the application of bio-medicine; moreover, the combination between optical tweezers and micro-mechanical sensor of bio-medical research is still rare seen between the countries.
The optical tweezers force was affected by laser and optical tweezers system. Therefore, it needs to be calibrated before you measure it. In the research, we measured force relative reference value by every size bead, and proposed a new sensing method, which used the loss of paralysis and active sperm trapping force to measure sperm mobility. Furthermore, proposed an application design combined two-photon absorption photo polymerization (TPP) to make lever biomedical sensors, which is a tool to measure cell stiffness, developing its feasibility of biomedical testing application on combination optical and TPP.
In the research, we focus on measured relative of laser power and trapping force plan by every size bead. This research can conduct laser tweezers force basis of any size micro particle element in the sensor. The study showed two main factors effect upon cell communication were the index of cell refraction and absorptive. Furthermore, proposed a new scheme used micro lever to measure cell stiffness, can sieve out and analyze cell stiffness in time. Thence laid the foundation of biological cells interaction research.
目錄
摘要 I
Abstract II
圖目錄 V
表目錄 VII
第一章 研究目的 1
1.1 前言 1
1.2 目的 2
1.3 方法 4
第二章 理論與研究背景介紹 7
2.1 雷射光鉗 7
2.1.1 光鉗原理 7
2.1.2 光鉗歷史 15
2.2 雙光子光致聚合微製造(TPP) 21
2.3 細胞介紹 28
2.3.1 精子 28
2.3.2 羊水細胞 31
2.3.3 癌化細胞與細胞勁度 34
2.4 槓桿 37
第三章 實驗方法 40
3.1 實驗架設 40
3.2 樣本製作 44
3.3 計算及測量方法 47
3.3.1 RO模型及EM模型理論值計算 47
3.3.2 脫離速度測量光鉗作用力 50
3.3.3 調制雷射功率測量精子游動力 53
第四章 結果與討論 54
4.1 理論值計算結果 54
4.2 微珠作用力測量結果 56
4.3 微珠作用力與細胞作用力結果分析 58
4.4 精子作用力測量結果 61
4.4.1 脫離速度測量精子游動力 62
4.4.2 調制雷射功率測量精子游動力 64
4.5生醫感測器應用之設計 66
第五章 結論與未來展望 75
5.1 結論 75
5.2 未來展望 76
參考文獻 78
參考文獻
[1]吳崇安,黃鈞正,邱爾德,“光學嵌住之理論探討,” 物理雙月刊, Vol. 22, No. 5, 2000, pp. 485-488.
[2]Ashkin A,“Acceleration and trapping of particles by radiation pressure,” Phys Rev Lett, Vol. 24, No. 4, 1970, pp.156-9.
[3]C. L. Lin,“Opto-Mechanical Application of Microstructured Materials : 2) Laser-driven polymer micro-sensors,” 2004, pp. 56.
[4]Van de Hulst H,“Light scattering by small particles,” New York: Dover, 1981.
[5]Ashkin A,“Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regime,” Biophys J, Vol. 61, No. 2, 1992, pp. 569-82.
[6]Gouesbet G, Maheu B, Gr''ehan G,“Light scattering from a sphere arbitrarily located in a gaussian beam, using a bromwich formulation,” Journal of the Optical Society of America A, Vol. 5, No.9, 1988, pp. 1427-43.
[7]Ashkin A, Dziedzic J, Bjorkholm J, Chu S,“Observation of a single-beam gradient force optical trap for dielectric particles,” Opt Lett, Vol. 11, No. 5, 1986, pp.288.
[8]Yamashita K., Murata A, Okuyama M,“Golay Cell Type of Miniaturized Infrared Sensor Using Si-Diaphragm,” Proc. Int. Conf. Solid-State Sensors and Actuators (Transducers’97), Chicago, June 16-19, 1997, pp.1067-1070.
[9]MC Hsieh, YK Fang, PM Wu, CC Yang, YC Lin, WD Wang, SF Ting, JJ Ho,“Design and fabrication of a novel crystal SiGeC far infrared sensor with wavelength 8-14 micrometer,” EEE Sensors Journal, Vol. 2, No. 4, 2002, pp. 360-5.
[10]Hudson Jr R, Hudson J,“The military applications of remote sensing by infrared,” Proc IEEE, Vol. 63, No. 1, 1975, pp. 104-28.
[11]Ashkin A, Dziedzic J, Yamane T,“Optical trapping and manipulation of single cells using infrared laser beams,” Nature, Vol. 330, No. 6105, 1987, pp. 769–771.
[12]Ashkin A,“Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regime,” Biophys J, Vol. 61, No. 2, 1992, pp. 569-82.
[13]Wright W. H, Sonek G. J. and Berns M. W,“Parametric study of the forces on microspheres held by optical tweezers,” Appl Opt, Vol. 33, 1994, pp. 1735.
[14]MC Hsieh, YK Fang, PM Wu, CC Yang, YC Lin, WD Wang, SF Ting, JJ Ho, “Design and fabrication of a novel crystal SiGeC far infrared sensor with wavelength 8-14 micrometer,” IEEE Sensors Journal, Vol. 2, No. 4, 2002, pp. 360-5.
[15]Ashkin A, Dziedzic J, Yamane T, “D Optical trapping and manipulation of single cells using infrared laser beams,” Nature, Vol. 33, No. 6150, 1987, pp. 769–771.
[16]MacDonald M, Paterson L, Volke-Sepulveda K, Arlt J, Sibbett W, Dholakia K, “Creation and manipulation of three-dimensional optically trapped structures,” Science, Vol. 296, No. 5570, 2002, pp. 1101-3.
[17]Galajda P, Ormos P, “Complex micromachines produced and driven by light,” Apply Phys Letts, Vol. 78, 2001, pp. 24.
[18]Kolluru GK, Tamilarasan K, Rajkumar AS, Geetha Priya S, Rajaram M, Saleem NK, Majumder S, Jaffar Ali B, Illavazagan G, Chatterjee S, “Nitric oxide/cGMP protects endothelial cells from hypoxia-mediated leakiness,” Eur J Cell Biol, 2007.
[19]Dharmadhikari J, Roy S, Dharmadhikari A, Sharma S, Mathur D, “Naturally occurring, optically driven, cellular rotor,” Appl Phys Lett, Vol. 85, 2004, pp. 6048.
[20]Tadir Y., Neev J. and Berns M. W., “Laser in Assisted Reproduction and Genetics,” Journal of Assisted Reproduction and Genetics, Vol. 9, No.4, 2005, pp. 303-305.
[21]Tadir Y, Wright WH, Vafa O, Ord T, Asch R, Berns MW, “Micromanipulation of sperm by a laser generated optical trap,” Fertil Steril, Vol. 52, 1989.
[22]Konig K, Svaasand L, Liu Y, Sonek G, Patrizio P, Tadir Y, Berns M, Tromberg B, “Determination of motility forces of human spermatozoa using an 800 nm optical trap,” Cell Mol Biol, Vol. 42, No. 4, 1996.
[23]Nascimento J. M., Shi L. Z., Meyers S., Gagneux P., Loskutoff N. M., Botvinick E. L., Berns M. W., “The use of optical tweezers to study sperm competition and motility in primates,” Journal of the Royal Society Interface, Vol. 5, No. 20, 2008, pp. 297-302.
[24]Goepper-Mayer M., “Ober Elementarakte mit zwei Quantensprungen,” Annalen der Physik, Vol. 9, No. 2, 1931., pp. 273-294.
[25]Kaiser W. and Garrett C. G. B., “Twophoton Excitation in CaF2:Eu2+,” Physical Review Letters, Vol. 7, No. 6,1961., pp. 229-231.
[26]潘恩亞,蒲念文,董玉平,游漢輝,“雙光子吸收光致聚合技術應用於微元件製作之研究,” Journal of C.C.I.T., Vol. 34, No. 1, 2005.
[27]C. L. Lin, “Opto-Mechanical Application of Micro structured Materials: 2) Laser-driven polymer micro-sensors,” 2004, pp. 76.
[28]Zissi, S., Bertsch, A, Jezequel, J.-Y., Corbel,S, Lougnot, D. J., and Andre, J. C.,“ Stereolithography and Microtechniques,” Microsystem Technology, Vol. 2, No. 2, 1996., pp.97-102.
[29]Horiyama, M., Sun, H.-B., Miwa, M., Matsuo, S., and Misawa, H., “Three-dimensional Microstructures Created by Laser Microfabrication Technology,” Japanese Journal of Applied Physics, Vol.38, No. 2B, 1999., pp. L212-L215.
[30]Maruo, S., Nakamura, O., and Kawata, S.,“Three-dimensional microfabrication with two-photon-absorbed photopolymerization,” Optics Letters, Vol.22, No. 2, 1997, pp. 132-134.
[31]Kawata, S., Sun, H.-B., Tanaka, T., and Takada, K., “Finer Features for Functional Microdevices,” Nature, Vol. 412, No. 6848, 2001., pp. 697-698.
[32]Hudson R. D. and Hudson J. W., “The military applications of remote sensing by infrared,” Proceedings of the IEEE, Vol. 1, 1975, pp. 104-128.
[33]Yamashita K., Murata A., and Okuyama M., “Go lay-cell type of miniaturized infrared sensor using Si-diaphragm,” Proceedings of TRANSDUCERS ''97 - the International Conference on Solid-State Sensors and Actuators, Vol. 2, 1997, pp. 1067-1070.
[34]M. C. Hsieh, Y. K. Fang, P. M. Wu, C. C. Yang, Y. C. Lin, W. D. Wang, S. F. Ting, and J. J. Ho, “Design and fabrication of a novel crystal SiGeC far infrared sensor with wavelength 8-14 micrometer,” IEEE Sensors Journal, Vol. 2, 2002, pp. 360-365.
[35]Li N., Tourovskaia A., and Folcb A., “Biology on a chip: microfabrication for studying the behavior of cultured cells,” Critical Review in Biomedical Engineering, Vol. 31, 2003, pp. 423-488.
[36]Park T. H. and Shuler M. L., “Integration of cell culture and micro fabrication technology,” Biotechnol. Prog, Vol. 19, 2003, pp. 243-253.
[37]Yun K. S., and Yoon E., “Micro/Nanofluidic Device for Single-Cell-Based Assay,” Biomedical Micro devices, Vol. 7, No. 1, 2005, pp. 35-40.
[38]Stroock A. D., Wheeler T. D., and Kirtland J., “Microfluidic relief for transport limitations,” BioTechniques, Vol. 39, No. 2, 2005, pp. 159-163.
[39]Huh D., Gu W., Kamotani Y.,. Grotberg J. B, and Takayama S., “Microfluidics for flow cytometric analysis of cells and particles,” Physiological Measurement, Vol. 26, No. 3, 2005, pp. 73-98.
[40]Boer G., Johann R., Rohner J., Merenda F., Delacrétaz G., Renaud P.,and Salathé R.P., “Combining multiple optical trapping with micro flow manipulation for the rapid bioanalytics on microparticles in a chip,” Review of Scientific Instruments, Vol. 78, 2007, pp. 116101.
[41]Tadir Y, Wright WH, Berns MW,“Cell micromanipulation with laser beams,” In G.I.F.T., From Basics to Clinics, GL Capitanio, RH Asch, L De Cecco, S Croce (eds). New York, Raven Press, 1989, pp. 359-368
[42]精子, 維基百科, http://zh.wikipedia.org.
[43]World Health Organization,“WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction,” 4th ed. London, Cambridge University Press, 1999.
[44]Siegel N., Valli A., “Stem cells in amniotic as new tools to study human genetic diseases,” Stem Cell Rev, Vol. 3, 2007, pp. 256-264.
[45]Weissman L., “Stem cells: Units of development, units of regeneration, and units in evolution,” Cell, Vol. 100, 2000, pp.157-168.
[46]Rosenthal N., “Prometheus’s vulture and the stem-cell promise,” New England Journal of Medicine, Vol. 349, 2003, pp. 267-274.
[47]Schulman A., “The search for alternative sources of human pluripotent stem cells,” Stem Cell Reviews, Vol. 1, 2005, pp.291-292.
[48]Wood A., “Ethics and embryonic stem cell research,” Stem Cell Reviews, Vol. 1, 2005, pp. 317–324.
[49]Kamm F. M., “Ethical issues in using and not using embryonic stem cells,” Stem Cell Reviews, Vol. 1, 2005, pp.325-330.
[50]Hoehn H. and Salk D., “Morphological and biochemical heterogeneity of amniotic fluid cells in culture,” Methods in Cell Biology, Vol. 26, 1982, pp.11-34.
[51]Gosden M., “Amniotic fluid cell types and culture,” British Medical Bulletin, Vol. 39, 1983, pp.348-354.
[52]Prusa A. and Hengstschläger M., “Amniotic fluid cells and human stem cell research-A new connection,” Medical Science Monitor, Vol. 8, 2002, pp.253-257.
[53]Fauza D., “Amniotic fluid and placental stem cells,” Best Practical Research in Clinical Obstetrics and Gynecology, Vol. 18, 2004, pp. 877-891.
[54]Milunsky A., “Amniotic fluid cell culture,” In A. Milunsky (Ed.) Genetic disorder and the fetus. New York: Plenum, 1979.
[55]Hoehn H., Salk D., “Morphological and biochemical heterogeneity of amniotic fluid cells in culture,” Methods in Cell Biology, Vol. 26, 1982, pp.11-34.
[56]Gosden C. M., “Amniotic fluid cell types and culture,” British Medical Bulletin, Vol. 39, 1983, pp. 348–354.
[57]Prusa A., Hengstschläger M., “Amniotic fluid cells and human stem cell research-A new connection,” Medical Science Monitor, Vol.8, 2002, pp.253-257.
[58]Fauza D., “Amniotic fluid and placental stem cells,” Best Practical Research in Clinical Obstetrics and Gynecology, Vol.18, 2004, pp.877-891.
[59]Guillot P. V., O’Donoghue K., Kurata H., Fisk N. M., “Fetal stem cells: Betwixt and between,” Seminars in Reproductive Medicine, Vol.24, 2006, pp.340-347.
[60]Sakuragawa N., Misawa H., Ohsugi K., Kakishita K., Ishii T., Thangavel R., “Evidence for active acetylcholine metabolism in human amniotic epithelial cells: Applicable to intracerebral all grafting for neurologic disease,” Neuroscience Letters, Vol.232, 1997, pp.53-56.
[61]Elwan M. A., Sakuragawa N., “Evidence for synthesis and release of catecholamine,” 1997.
[62]Guillot P. V., O’Donoghue K., Kurata H., and Fisk N. M., “Fetal stem cells: Betwixt and between,” Seminars in Reproductive Medicine, Vol. 24, 2006, pp.340–347.
[63]Mitka M., “Amniotic cell show promise for fetal tissue engineering,” The Journal of the American Medical Association, Vol. 286, 2001, pp. 2083.
[64]癌症, 維基百科, http://zh.wikipedia.org.
[65]Matsudaira Paul T., Lodish Harvey F., Arnold Berk, Kaiser, Chris, Monty Krieger, Matthew P Scott, Anthony Bretscher, Hidde Ploegh, “Molecular Cell Biology,” W.H.Freeman & Co Ltd, 2007.
[66]Alberts, Bruce, “Molecular biology of the Cell,” T Garland Science, New York, 2002.
[67]Frei, Emil; Kufe, Donald W.; Holland, James F, “Cancer medicine 6.Hamilton, Ont,” BC Decker, 2003.
[68]Chakras G. T., Lehenkari P. P., Horton M. A. “Atomic force microscopy can be used to mechanically stimulate osteoblasts and evaluate cellular strain distributions”, Ultramicroscopy , Vol. 86, 2001, pp.85-95.
[69]Chung Te Hsuan, “Investigation of Cell Stiffness: Atomic Force Microscopy and Finite Element Analysis,” Partial Fulifillment of the Requirements for the Degree of Master of Science, 2006.
[70]Stamenovic D., Ingber D. E., “Model of cytoskeletal mechanics of adherent Cells”, Biomechan Model Mechanobiol, 2002, pp.95-108.
[71]Dennerll T.J., “Tension and compression in the cytoskeleton of PC-12 neurites. II: Quantitative measurements.” J Cell Biol, Vol. 107, No. 2, 1988, pp.665-74.
[72]Wang N., “Cell prestress. I. Stiffness and prestress are Closely associated in adherent contractile cells”, Am J Physiol Cell Physiol, Vol. 282, No.3, 2002, pp. C606-16.
[73]Samarendra K., Mohanty, Abha Uppal, and Pradeep K. Gupta, “Self-rotation of red blood cells in optical tweezers: prospects for high throughput malaria diagnosis,” Biotechnology Letters, Vol.26, 1997, pp. 971-974.
[74]Sarah E. Cross, Yu-Sheng Jin, Jianyu Rao and James K. Gimzewski, “Nanomechanical analysis of cells from cancer patients,” Published online, Vol. 10, 2007, pp. 1038.
[75]槓桿, 維基百科, http://zh.wikipedia.org.
[76]Hale, G.M and Querry, M R, “Optical constants of water in the 200-nrn to 200-mm wavelength region,” Appl. OpL, vol. 12, 1973, pp. 555-563.
[77]Karsten Konig, Yona Tadir, PasquaUe Patrizio,Michael W.Berns and Bruce J.Tromberg, “Effects of ultraviolet exposure and near infrared laser tweezers on human spermatozoa,” Human Reproduction, Vol. 11, No. 12, 1996, pp. 2162-2164.
[78]Youli Yu, Zhang Lei, Zhenxi Zhang, “Computation for Optical Trap Stiffness Versus Refractive Index of Biological Cell”, ACTA OPTICA SINICA, Vol. 27, No. 6, 2001, pp.85-95.
[79]劉益年, 美商必帝股份有限公司, “流式細胞儀工作原理及儀器分析”.
[80]Tadir Y, Wright WH, Vafa O, Ord T, Asch R, Berns MW, “Micromanipulation of sperm by a laser generated optical trap,” Fertil Steril, Vol.52, 1989, pp. 870473.
[81]Colon JM, Sarosi P, McGovern PG, Ashkin A, Dziedzic JM, Skurnick J, Weiss G, Bonder EM, “Controlled micromanipulation of human spermatozoa in three dimensions with an infrared laser optical trap: Effect on sperm velocity,” Fertil Sterit, Vol.57, 1992, pp. 695-698.
[82]Konig K, Svaasand L, Liu Y, Sonek G, Patrizio P, Tadir Y, Berns M, Tromberg B, “Determination of motility forces of human spermatozoa using an 800 nm optical trap,” Cell Mol Biol, Vol. 42, No. 4, 1996.
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