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研究生:黃郡瑋
研究生(外文):Huang Chun Wei
論文名稱:絲瓜機能性成分分析暨其抗氧化及抗發炎之研究
論文名稱(外文):Analysis of functional components in Luffa Cylindrica and its antioxidant and anti-inflammatory activities
指導教授:高彩華
指導教授(外文):Kao Tsai Hua
口試委員:林璧鳳吳文勉
口試委員(外文):Lin Bi FongWu Wen Mein
口試日期:2011-01-26
學位類別:碩士
校院名稱:輔仁大學
系所名稱:食品科學系
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2011
畢業學年度:99
語文別:中文
論文頁數:159
中文關鍵詞:絲瓜抗氧化抗發炎
外文關鍵詞:luffaantioxidantanti-inflammation
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絲瓜(Luffa cylindrica (L.) Roem)為台灣常食用蔬菜之一,亦可作為傳統藥材,然而,關於絲瓜機能性成分、抗氧化及抗發炎效果尚未完全瞭解,因此本研究目的即在分析絲瓜萃取物中之總酚、總類黃酮素、皂苷、類胡蘿蔔素和葉綠素等機能性成分含量,進一步製備萃取物探討其抗氧化及抗發炎之效果。結果顯示,分別以水、乙醇及乙酸乙酯與絲瓜皮及瓜肉比例為1:50 (w/v)時,總酚萃出效果最佳。每毫升萃取液中,總酚含量以瓜皮水萃取液最高,總類黃酮素、齊敦果酸、類胡蘿蔔素及葉綠素則以瓜皮乙醇萃取液最多。每克乾重中,同樣分別以瓜皮水萃取物及瓜皮乙醇萃取物含較高量總酚及總類黃酮素,但其它成分則以瓜皮乙酸乙酯萃取物含量最高。抗氧化活性方面,瓜皮水萃取液之螯合力及還原力最好,瓜皮乙醇萃取液之清除自由基能力較高,除瓜肉水萃取液之外,其它萃取液皆可有效抑制共軛雙烯生成,瓜皮和瓜肉之乙酸乙酯萃取液均可有效抑制TBARS生成。抗發炎部分則顯示,瓜皮和瓜肉之乙醇及乙酸乙酯萃取液均可抑制一氧化氮生成,瓜皮和瓜肉之乙酸乙酯萃取液能夠降低iNOS蛋白質表現和IκBα磷酸化程度。絲瓜皮和瓜肉之乙醇萃取液可降低PGE2濃度,但對COX-2蛋白質表現無抑制效果。瓜皮和瓜肉之水萃取液及瓜皮乙酸乙酯萃取液有效抑制IL-1β生成,而所有的絲瓜萃取液皆顯著(p<0.05)抑制IL-6生成,但對TNF-α均無抑制效果。西方墨點法結果顯示,瓜肉乙酸乙酯萃取液可降低ERK磷酸化程度,然而,所有萃取液對均無法抑制JNK磷酸化。
Luffa cylindrica (L.) Roem, a vegetable frequently consumed in Taiwan, has been used as a traditional medicine. However, the composition of functional components in luffa and their antioxidant and anti-inflammatory activities have not been completely explored. The objectives of this study were to determine the content of phenolics, flavonoids, saponins, carotenoids and chlorophylls in luffa extracts, and evaluate their antioxidant as well as anti-inflammatory activities. The extraction efficiency of total phenolics of luffa peel and fruit with water, ethanol and ethyl acetate was found to be maximum at a sample-to-solvent ratio of 1:50 (w/v). Of the various functional components present per mL of extract, phenolics were present in large amount in the aqueous extract of peel, while flavonoids, oleanolic acid, carotenoids and chlorophylls dominated in the ethanolic peel extract. For their levels per gram of the extract, phenolic and flavonoids were found to be abundant in aqueous and ethanolic extracts of peel and fruit, respectively. However, the contents of other functional components were highest in the ethyl acetate extract of peel. On comparing the antioxidant activity of each solvent extract, the aqueous extract of peel showed the greatest reducing power and Fe (II) chelating ability, while the ethanolic extract of peel exhibited a large TEAC value and a high DPPH free radical scavenging activity. With the exception of aqueous fruit extract, the other solvent extracts of fruit and peel effectively reduced the formation of conjugated diene. The ethyl acetate extract of both peel and fruit were also found effective in inhibiting the TBARS. For anti-inflammatory activity, the ethanolic and ethyl acetate extracts of both peel and fruit decreased the production of nitric oxide. In addition, the ethyl acetate extracts of peel and fruit suppressed iNOS protein expression and IκBα phosphorylation, while their ethanolic extracts reduced the secretion of prostaglandin E2 without any change in COX-2 protein expression. The aqueous extracts of both peel and fruit as well as the ethyl acetate extract of peel inhibited the production of interleukin 1.All solvent extracts of both peel and fruit significantly (p<0.05) inhibited interleukin 6 production, whereas it had no inhibitory effect on the production of tumor necrosis factor. Western blot analysis revealed ethyl acetate extract of fruit to decrease ERK phosphorylation levels. Nevertheless, the JNK phosphorylation was not inhibited by different solvent extracts of peel and fruit.
第一章、文獻回顧
一、絲瓜簡介
(一)絲瓜之生長分佈
(二)絲瓜之化學成分
(三)絲瓜之生理活性
二、發炎反應簡介
(一)巨噬細胞與脂多醣
(二)促發炎反應相關因子
(三)發炎反應相關訊息傳遞路徑
(一)類黃酮素之結構
(二)類黃酮素之生理活性
(三)類黃酮素之抗發炎機制
四、皂苷簡介
(一)皂苷之結構
(二)皂苷之生理活性
(三)皂苷之抗發炎機制
第二章、材料與方法
ㄧ、研究架構
(一)原料
(二)化學藥品
(三)固相萃取管
(四)儀器設備
三、實驗方法
(一)絲瓜萃取液製備方法評估
(二)絲瓜萃取液中機能性成分之測定
(三)絲瓜萃取液抗氧化之效果
(四)絲瓜萃取物之抗發炎活性
(五)統計分析
第三章、結果與討論
ㄧ、絲瓜粉末與萃取溶劑比例之評估
二、機能性成分分析
(ㄧ)總酚、總類黃酮之檢測
(二)維他命C及α-生育醇之檢測
(三)皂苷之檢測
(四)類胡蘿蔔素之檢測
(五)葉綠素之分析
三、絲瓜萃取液抗氧化之效果
(一)螯合亞鐵離子能力
(二)還原力測定
(三)清除DPPH自由基之能力
(四)TEAC值
(五)抑制共軛雙烯
(六)TBARS之抑制效果
四、絲瓜萃取液抗發炎之效果
(一)機能性成分含量
(二)細胞存活率
(三)一氧化氮及相關蛋白質結果
(四)PGE2及其相關蛋白質結果
(五)IL-1β及IL-6及其相關蛋白質結果
(六)TNF-α及其相關蛋白質結果
第四章、結論
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