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研究生:陳宗明
研究生(外文):Chen Tsung-Ming
論文名稱:二氧化氯空氣燻蒸消毒動力學探討
論文名稱(外文):Kinetic study of chlorine dioxide gas disinfection on bacillus Atrophaeus spores
指導教授:王文華王文華引用關係
指導教授(外文):Wang Wen-Hua
學位類別:碩士
校院名稱:中華醫事科技大學
系所名稱:生物安全衛生研究所
學門:醫藥衛生學門
學類:公共衛生學類
論文種類:學術論文
畢業學年度:96
語文別:中文
論文頁數:42
中文關鍵詞:二氧化氯空氣除污枯草桿菌孢子
外文關鍵詞:chlorine dioxideair decontaminationBacillus atrophaeus spores
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本研究是利用經採用避光處理的壓克力材質製作的環境模擬暴露艙進行二氧化氯氣體對枯草桿菌孢子之空氣燻蒸消毒效能動力學探討。首先,利用市售的二氧化氯溶液經活化後加以稀釋並配製不同的濃度,再以超音波霧化器進行噴霧汽化後輸送進入暴露艙內,利用氣體檢知管進行空氣採樣分析以了解其濃度之時間變化情形,接著再利用經過高溫滅菌處理的棉線將含有枯草桿菌孢子(Bacillus Atrophaeus Spores)的生物指示孢菌片以固定間隔串接並均勻分散在艙內燻蒸消毒不同時間後取出放入TSB (Trypticase Soy Broth)培養液中觀察是否呈現渾濁的陽性反應率,之後再以無菌稀釋液進行十倍數逐步稀釋後以表面塗抹法完成二重覆樣本培養計數分析。結果顯示,二氧化氯溶液配製濃度不超過5000 mg/L時具有良好的線性關係,空氣中二氧化氯氣體濃度的半衰期約介於4-5小時之間,枯草桿菌孢子生物指示劑試紙存活之菌落生成數經過以對數尺度處理後,與二氧化氯氣體燻蒸消毒作用劑量間具有良好的線性相關。經計算其在100 ppm濃度之D值(Decimal reduction time)約為60分鐘,建議每次燻蒸消毒時間最好不超過2小時,根據二氧化氯氣體燻蒸消毒作用劑量可以推估要達到6 log量級之燻蒸循環次數,可提供規劃進行室內空氣消毒之參考依據。
The aim of this experiment is to study the inactivation kinetic of Bacillus atrophaeus spores with chlorine dioxide gas in a test chamber. Bacillus atrophaeus spores strips were treated with chlorine dioxide gas which was aerosolized by ultrasonic nebulizer from chlorine dioxide solution and introduced into test chamber at different concentrations in different time to determine the inactivated rate using enumeration and fractionation analysis of tryptic soy broth (TSB) and tryptic soy agar (TSA). The exposure dose was calculated by Harber’s rule based on chlorine dioxide gas concentration which was measured by detector tubes and total exposure time to establish D-value (Decimal reduction time) from inactivation curve. It was found that the half life of chlorine dioxide gas was less than 5 hr (300 min) at 20~25 ℃ under 90~95% relative humidity. The number of survived spores after exposure to chlorine dioxide gas followed an exponential decrease with increasing exposure concentration and contact time. The D-value of Bacillus atrophaeus spores treated with 100 ppm chlorine dioxide gas was about 60 minutes in this study. The results from this study suggest that gaseous chlorine dioxide should be a highly effective decontaminating agent for Bacillus atrophaeus spores within 2hr exposure time to determine the sterilization cycle achieving 6-log reduction.
第一章 前言 1
1-1 研究背景 1
1-2 研究目的 1
第二章 文獻探討 3
2-1 二氧化氯的理化特性及其應用 3
2-2 二氧化氯進行飲用水水質處理的優點 5
2-3 空氣燻蒸消毒劑的類型 6
2-4 二氧化氯消毒作用機轉之研究 9
2-5 二氧化氯氣體對蔬菜水果中微生物殺菌效果的相關研究 10
2-6 二氧化氯的空氣消毒效果 14
2-7 二氧化氯暴露的健康危害 15
第三章 材料與方法 17
3-1 實驗設備器材及藥品 17
3-2 無菌稀釋液製備程序 21
3-3 二氧化氯穩定時間測試 22
3-4 二氧化氯燻蒸消毒實驗步驟 23
3-5 數據分析處理 24
第四章 結果與討論 29
4-1 二氧化氯溶液與燻蒸氣體濃度之關係 29
4-2 二氧化氯氣體濃度降解分析結果 30
4-3 燻蒸消毒作用劑量與孢子存活率分析 31
第五章 結論與建議 36
5-1 結論 36
5-2 建議 37
參考文獻 39
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