本研究目的是要找出適用於常見之生物反應器的最佳化操作模式，因此測試生物濾床、生物洗滌塔和平板式生物洗滌塔對PGMEA的去除效果。當PGMEA的進流濃度在100~300ppm，滯留時間為20秒時，觀察到生物濾床和生物洗滌塔分別有98%和93%的平均移除效能，移除負荷分別為 212 g/m3/hr and 258 g/m3/hr。平板式洗滌塔方面，當滯留時間為20秒，進流濃度在100~300ppm時，其平均移除率可以達到93%，移除負荷為150 g/m3/hr。
在生物洗滌塔和平板式生物洗滌塔方面，探討不同初始植種量對去除PGMEA之影響，結果顯示，當初始菌株植種量為109 CFU/mL時，對生物洗滌塔的影響較為顯著，使得反應器貫穿後即可達到90%以上的去除效果，但對平板式生物洗滌塔的影響較不明顯。當添加不同的濾料至生物濾床和生物洗滌塔時，發現添加額外的濾料至生物洗滌塔，可以縮短生物洗滌塔達到90%以上去除率所需的時間，但是對於生物濾床的影響則不顯著。對不同液氣比之研究方面，發現生物洗滌塔之灑水量為800 mL/min時，去除PGMEA的能力與灑水量為1900 mL/min相當，平均去除率皆可高達97%以上；平板式生物洗滌塔每分鐘灑水量為1900 mL時，平均去除率為99%，即使灑水量縮減為800 mL/min，待反應器運作五天左右，其去除率仍可達到98%以上，顯示兩者皆能有效且穩定地去除PGMEA。以不同氮源培養V1菌株之實驗結果顯示，以(NH4)2SO4作為培養基的氮源令V1菌株生長較為迅速，與其他培養基配方相比快約一倍的速率，可以大幅縮短反應器固定化時間，減少被汙染的機會。
本研究結果所得之最佳化操作參數，有助於實場規模反應器的建造，以提高整體運作效率並縮短固定化時間，達到最有效的運作方式。

This research is aimed to establish an effective biofiltration process which applys to remove the water-soluble organic waste gases emission from semiconductors and photonics industry. The target pollutant is focus on propylene glycol monomethyl ether acetate (PGMEA), which is harmful for human body, especially for liver and kidney.
The objective of this study is to estimate the removal efficiency in different conditions by three kinds of biofiltration processes. The data showed that it could remove 100-300 ppm PGMEA for 98% removal efficiency (RE) in biofilter system, 93% in bioscrubber system with retention time (RT) in 20 seconds, and 93% RE in plate membrane bioscrubber system with RT in 30 seconds. The elimination capacity achieved 212, 258 and 150 g-PGMEA/m3/hr for biofilter, bioscrubber and plate membrane bioscrubber, respectively. In addition, to study the effect of amount of seeding in bioscrubber and plate membrane bioscrubber. It showed that high amount of seeding (over 109 CFU/ml) was helpful for high RE (up to 98%) and stable for operation. Finally, we estimated the addition of extra packing materials in biofilter and bioscrubber. The data showed that the bioscrubber with extra packing materials could achieve RE 90% in short time, while there was no difference in biofilter.
Therefore, the optimal operation parameters for removal of PGMEA were obtained, and it could establish field-scale bioreactors for application in industrial field.

中文摘要 i
英文摘要 ii
誌謝 iii
目錄 iv
表目錄 ix
圖目錄 x
符號說明 xii
一、 前言 1
1.1. 研究背景及動機 1
1.2. 研究目的 1
二、 文獻回顧 4
2.1. 揮發性有機化合物排放來源 4
2.2. 丙二醇甲醚醋酸酯的來源與特性 4
2.3. 丙二醇甲醚醋酸酯對人體的危害 4
2.4. 揮發性有機化合物常用的去除方式 4
2.4.1. 焚化法(Incineration) 4
2.4.2. 吸附法與吸收法(Adsorption and absorption) 5
2.4.3. 進階氧化程序(Advanced oxidation processes) 5
2.5. 生物處理法 (Biological treatment) 6
2.6. 生物處理法之類型 6
2.6.1. 生物濾床 6
2.6.2. 生物滴濾床 7
2.6.3. 生物洗滌塔 7
2.7. 影響生物反應器的因子 7
2.7.1. 揮發性有機化合物之類型 7
2.7.2. VOCs的濃度 8
2.7.3. 進氣的流量 8
2.7.4. 使用之微生物種類 8
2.7.5. 營養源 8
2.7.6. 溫度與pH值 9
三、 材料與方法 10
3.1. 實驗藥品 10
3.2. 儀器設備 10
3.3. 菌株來源 11
3.4. 反應器架設 11
3.4.1. 曝氣系統 11
3.4.2. 反應器本體 11
3.5. 菌數分析 12
3.6. 化學需氧量分析 12
3.7. 移除負荷分析(Elimination capacity) 12
3.8. 管柱實驗-生物濾床 13
3.9. 管柱實驗-生物洗滌塔 13
3.10. 空白吸附實驗-生物濾床 13
3.11. 空白吸附實驗-生物洗滌塔 13
3.12. PGMEA氣體濃度分析 14
3.13. PGMEA氣體濃度之檢量線製作 14
3.14. PGMEA液體濃度之檢量線製作 14
3.15. 實驗操作 15
3.15.1. 生物濾床去除PGMEA實驗 15
3.15.2. 生物洗滌塔去除PGMEA實驗 15
3.15.3. 平板生物洗滌塔去除PGMEA實驗 15
3.15.4. 不同溫度對V1菌株生長速率之影響 16
3.15.5. 突變株篩選 16
3.15.6. 不同的初始菌株植種量對PGMEA去除效率之影響 16
3.15.7. 不同的固定化載體對PGMEA去除效率之影響 16
3.15.8. 不同液氣比對PGMEA去除效率之影響 16
3.15.9. 不同氮源對V1生長速率之影響 17
四、 實驗結果與討論 18
4.1. 生物濾床實驗 18
4.1.1. 滯留時間之影響 18
4.1.2. 進流濃度之影響 18
4.1.3. 移除負荷 19
4.2. 生物洗滌塔實驗 19
4.2.1. 滯留時間之影響 19
4.2.2. 進流濃度之影響 21
4.2.3. 移除負荷 21
4.3. 平板式生物洗滌塔實驗 21
4.3.1. 進流負荷對去除PGMEA效能之影響 21
4.3.2. 移除負荷 22
4.4. 不同溫度對V1菌株生長速率之影響 23
4.5. 突變株篩選 23
4.6. 不同的初始菌株植種量對PGMEA去除效率之影響 23
4.6.1. 生物洗滌塔 23
4.6.2. 平板式生物洗滌塔 24
4.7. 不同固定化載體對PGMEA去除效率之影響 24
4.7.1. 生物濾床 24
4.7.2. 生物洗滌塔 24
4.8. 不同液氣比對PGMEA去除效率之影響 25
4.8.1. 生物洗滌塔 25
4.8.2. 平板式生物洗滌塔 25
4.9. 不同氮源對V1生長速率之影響 26
五、 結論 27
5.1. 以生物濾床處理PGMEA 27
5.2. 以生物洗滌塔處理PGMEA 27
5.3. 以平板式生物洗滌塔處理PGMEA 27
5.4. 菌株植種量與固定化濾料之影響 27
5.5. 液氣比及氮源之影響 28
5.6. 整體優勢 28
5.7. 未來展望 28
六、 參考文獻 29

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