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研究生:陳律方
研究生(外文):CHEN, LU-FANG
論文名稱:地骨皮化學成分及藥理活性之研究
論文名稱(外文):Phytochemical and Pharmacological Studies on
指導教授:林漢欽林漢欽引用關係張溫良
學位類別:碩士
校院名稱:國防醫學院
系所名稱:藥學研究所
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
中文關鍵詞:地骨皮枸杞巨噬細胞
外文關鍵詞:G-CSFgranulocyte colony-stimulating factorLycium chinense Mill.
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地骨皮 (Cortex Lycii Radicis) 為茄科 (Solanaceae) 植物枸杞 (Lycium
chinense Mill.) 或寧夏枸杞 (L. barbarum Linn.) 的乾燥根皮。在傳統作為清
熱藥使用,現代藥理多用在解熱、降血壓和降血糖作用[1]

取地骨皮經鑑定後以酒精萃取後,依其極性大小分為正己烷、二氯甲
烷、乙酸乙酯、正丁醇及水可溶層等五部份。 地骨皮分配層在體外誘發巨
嗜細胞株分泌G-CSF (granulocyte colony-stimulating factor) 之活性試驗發
現,正己烷層 (LC-H) 及乙酸乙酯層 (LC-E) 具有頗強的誘發活性,因此將
正己烷層與乙酸乙酯層分別經由正、逆相管柱層析分離純化得到12個化合
物,其中含1個sesquiterpene 為 (10Z, 12Z)-dihydrophaseic acid (1);4個
steroid及其glucosides分別為stigmast-4-en-3-one (2)、ergosterol peroxide (3)
、β-sitosterol (4)、β-sitosterol-3-O-β-D-glucoside (5);4個phenylpropanoid分
別為methyl eugenol (6)、eicosyl ferulate (7)、trans-p-coumaric acid (8)、
trans-caffeic acid (9);2個benzenoid為vanillic acid (10)、p-hydroxybenzoic
acid (11);及trans-N-caffeoyltyramine (12)。 上述成分中,化合物1為新化合
物,化合物2、3和7則是首次由地骨皮中分離得到。
而由此兩個活性層分離純化出之12個化合物中,由正己烷層 (LC-H)
所分離的純化合物K01、K02及K03,和由乙酸乙酯層 (LC-E) 所分離的純
化合物K07、K08、K09及K10等七個化合物誘發巨噬細胞株分泌G-CSF之
活性非常顯著,其作用機轉及SAR值得進一步研究討論。
第一章 緒論
第一節 研究背景 1
第二節 研究目的 4
第三節 植物概述 5
第四節 化學成分回顧 6
第五節 藥理活性回顧 13
第二章 化學成分抽取與分離
第一節 抽取分層方法 14
第二節 化學成分之分離與純化 15
第三章 化學結構鑑定
第一節 (10Z, 12Z)-dihydrophaseic acid (1) 之結構鑑定 17
第二節 stigmast-4-en-3-one (2) 之結構鑑定 20
第三節 ergosterol peroxide (3) 之結構鑑定 22
第四節 methyl eugenol (6) 之結構鑑定 24
第五節 eicosyl ferulate (7) 之結構鑑定 26
第六節 trans-p-coumaric acid (8) 之結構鑑定 29
第七節 trans-caffeic acid (9) 之結構鑑定 31
第八節 vanillic acid (10) 之結構鑑定 33
第九節 p-hydroxybenzoic acid (11) 之結構鑑定 35
第十節 trans-N-caffeoyltyramine (12) 之結構鑑定 37
第四章 誘發顆粒性白血球刺激因子(G-CSF)試驗
第一節 粗抽物誘發RAW 246.7細胞株分泌G-CSF之活性篩選結果 39
第二節 純化合物誘發RAW 246.7細胞株分泌G-CSF之活性篩選結果 41
I章次 節次 標題 頁數
第五章 實驗方法
第一節 材料與儀器 45
第二節 抽取與萃取 49
第三節 分離與純化 50
第四節 誘發RAW 246.7細胞株分泌G-CSF之活性篩選方法 53
第五節 各化合物之物理及光譜數據 55
第六章 結論 59
參考文獻 60
附圖 65
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