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研究生:陳雅琦
研究生(外文):YaChi Chen
論文名稱:近中性電解水對數種植物種子萌發與種苗生長之影響
論文名稱(外文):The Effects of neutral electrolyzed oxidizing water on seed germination and seedling growth of several plants
指導教授:鄔家琪
指導教授(外文):Chia-Chyi Wu
學位類別:碩士
校院名稱:國立宜蘭大學
系所名稱:園藝學系碩士班
學門:農業科學學門
學類:園藝學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
論文頁數:70
中文關鍵詞:無隔膜電解水抑菌種苗芽菜銹病
外文關鍵詞:membraneless electrolyzed waterdesinfectionseedlingsproutrust
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本研究使用無隔膜電解水進行蘿蔔(Raphanus sativus L.)、小白菜(Brassica chinensis L.)、苜蓿(Medicago sativa L.)、豌豆(Pisum sativum L.)、甜瓜(Cucumis melo L.)、南瓜(Cucurbita moschata Duchesne)、水稻(Oryza sativa L.)、小麥(Triticum turgidum L.)及青蔥(Allium fistulosum L.)等九種種子的浸種,觀察濃度與時間對其萌發的影響,並以電解水噴灑種苗,觀察對其生長的影響。對苜蓿芽等芽苗菜以電解水清洗抑菌,觀察對其總生菌數降低之效應。再以無隔膜電解水噴灑,進行於土壤種植青蔥之銹病防治可行性評估。結果顯示,以不同有效氯濃度浸種不同時間,對種子發芽率並無顯著影響,但濃度達1000 mg‧L-1之電解水浸種會影響種子之胚軸長度。電解水浸種後之種子在種植後十四天之萌發率與實驗室中之發芽率各處理間相近,且種植兩週內對地上部之生長無明顯影響。以電解水噴灑處理蘿蔔、小白菜、甜瓜、南瓜、苜蓿、豌豆及小麥種苗,兩週後調查其生育結果,發現100及200 mg.L-1處理組之鮮重、株高、根重均無顯著差異,而1000 mg.L-1電解水與漂白水處理組之鮮重與株高最低,葉片中葉綠素含量也最低,可溶性蛋白質含量則隨種類而有不同改變。以電解水進行芽苗菜的清洗抑菌,結果顯示25 mg.L-1之電解水清洗苜蓿芽可降低起始總生菌數 1.59 log CFU.g-1 ,而50 mg.L-1之電解水清洗則可降低總生菌數 2.01 log CFU.g-1。以電解水清洗豌豆苗及小麥草的起始總生菌數也分別可降低 0.9 ~ 1.37 log CFU.g-1。經電解水清洗處理過後,芽苗菜在4天之儲存期間總生菌數增加量少於蒸餾水處理組及對照組,顯示以電解水清洗可維持其抑菌效果,符合一般儲架販售需求。對青蔥銹病之防治效果顯示,以200及1000 mg.L-1之電解水噴灑的方式不能顯著減少病斑的數量,但可使銹孢子呈現一定程度去活化現象。無隔膜電解水可應用在種子及芽苗菜的抑菌,減少種苗病害發生及芽苗菜的總生菌數;且對蔬菜病害,如青蔥銹病的防治有一定的效果,是可考慮採用的抑菌材料。
The effect of electrolyzed oxidizing (EO) water on seed imbibitions of 9 crops, including radish, Chinese cabbage, pea, alfalfa, melon, pumpkin, wheat, rice and green onion, was investigated. The germination test showed that the available chlorine and imbibitions time would not affect the germination percentage. But EO water of 1000 mg.L-1 available chlorine would decrease the elongation of hypocotyles. The effect of 100, 200 and 1000 mg.L-1 EO water applied as a foliar spray three times per week on seedlings of radish, Chinese cabbage, melon, pumpkin, alfalfa, pea, and wheat under planting in soil conditions in two weeks is studied. The result showed the mass and height of seedlings didn’t decrease significantly, but 1000 mg.L-1 EO water spraying would decrease the content of chlorophyll dramatically, and the content of total soluble proteins changes depending on species. The effectiveness of total plate count (TPC) reduction of EO water washing on sprouting vegetables was also been studied. For freshly harvested alfalfa, pea, and wheat sprouts on which TPC were initially 8.03, 7.85, and 7.24 log CFU‧g-1, respectively. Disinfection with 25 mg.L-1 EO water reduced TPC on alfalfa sprouts by 1.59 log CFU‧g-1, and 50 mg‧L-1 EO water reduced TPC by 2.01 log CFU.g-1. On pea and wheat sprouts TPC reduced by 0.9 ~ 1.37 log CFU.g-1. Comparative to distilled water treated and non treated sample, EO-treated sample did not show significant increase of TPC during storage. 50 mg.L-1 EO water applied as a foliar spray three times per week on green onion under field conditions to the prevention and control of rust did not produce any visible phytoxicity. Therefore, using EO water for decontamination of seed and sprouting vegetables was recommended to be an effective method of controlling microflora.
摘要.... I
Abstract....II
表目錄....IV
圖目錄....V
壹、前言....1
貳、前人研究....2
一、電解水之原理與特性....2
二、電解水在作物種植上之應用....4
三、電解水在蔬菜清洗之應用....8
參、材料與方法....10
一、無隔膜電解水生成....10
二、無隔膜電解水浸種消毒濃度對蘿蔔等作物種子萌發的影響....10
三、無隔膜電解水浸種消毒時間對蘿蔔等作物種子萌發的影響....11
四、以無隔膜電解水浸種消毒,對蘿蔔等植株幼苗生育的影響....12
五、電解水噴灑處理對作物幼苗生長之影響....12
六、無隔膜電解水對芽苗菜微生物的清洗殺菌效果....14
七、無隔膜電解水對青蔥銹病之預防與去除效果....14
肆、結果....16
一、無隔膜電解水浸種消毒濃度對蘿蔔等作物種子發芽的影響....16
二、無隔膜電解水浸種消毒時間對蘿蔔等作物種子萌發的影響....26
三、以無隔膜電解水浸種消毒,對蘿蔔等植株幼苗生育的影響....30
四、電解水噴灑處理,對蘿蔔等作物幼苗生長的影響....39
五、電解水對芽苗菜微生物的清洗殺菌效果....47
六、電解水對青蔥銹病之預防與去除效果....52
伍、討論....53
ㄧ、電解水浸種消毒濃度及時間對蘿蔔等作物種子萌發的影響....53
二、以無隔膜電解水浸種消毒對蘿蔔等作物植株生育的影響....54
三、電解水噴灑處理對作物幼苗生長之影響....55
四、電解水對芽苗菜微生物的清洗殺菌效果....56
五、電解水對青蔥銹病之預防與去除效果....57
參考文獻....59
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