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研究生:張嘉渝
論文名稱:探討熱處理對衣藻銅離子耐受性之影響
論文名稱(外文):The effect of heat pretreatment on copper-tolerance of Chlamydomonas reinhardtii
指導教授:徐邦達徐邦達引用關係
學位類別:碩士
校院名稱:國立清華大學
系所名稱:生物資訊與結構生物研究所
學門:生命科學學門
學類:生物訊息學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
論文頁數:63
中文關鍵詞:衣藻
相關次數:
  • 被引用被引用:3
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植物界常見現象—「逆境共耐性」意指當植物體在面對一個短暫且不致命的逆境後,會對另一個逆境產生較好的防禦力。
衣藻細胞 Chlamydomonas reinhardtii 經過 40℃ 加熱兩小時後給予銅處理,並使用 H2DCFDA 為染劑,以流式細胞儀偵測細胞內 ROS 的含量,發現未熱處理的細胞會隨著銅濃度梯度產生大量 ROS,而事先經熱處理的細胞則可大幅降低此 ROS 的產生。以掃描式共軛焦顯微鏡,也觀察到相同結果。
為更深入探討熱處理是否透過一新合成蛋白質,使衣藻在遭受銅逆境時減少 ROS 產生,我們以 cycloheximide 進行前處理,發現熱處理降低 ROS 產生的效果消失。以 HSP70 單株抗體 3a3 進行 western blot,發現熱處理細胞的 HSP70 表現量較未熱處理細胞高。
此外,在我們的研究中也發現,事先熱處理可降低銅離子對光合作用活性的傷害,但卻無法挽回葉綠素含量的受損,同時細胞死亡程度也會增加。
綜合以上結果,雖然事先熱處理可誘發衣藻產生大量 HSP70,進而減少衣藻在銅逆境下 ROS 的產生,但細胞終究還是會走向死亡一途。說明熱逆境所產生的防禦力不足以使衣藻能夠完全地抵抗銅逆境的毒害。
摘要-------------------------------------------------------1
前言-------------------------------------------------------4
材料與方法--------------------------------------------8
結果-------------------------------------------------------14
討論-------------------------------------------------------18
圖表-------------------------------------------------------23
附表-------------------------------------------------------55
參考文獻------------------------------------------------59

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