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研究生:許文瀚
論文名稱:電解海水應用於兩種石斑病毒之不活化與洗卵消毒之效果評估
論文名稱(外文):Efficacy of electrolyzed seawater on virus inactivation of two grouper (Epinephelus sp.) viruses and egg disinfection of grouper
指導教授:周信佑周信佑引用關係
指導教授(外文):Hsin-Yin Chou
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:水產養殖學系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
論文頁數:81
中文關鍵詞:電解海水病毒不活化洗卵
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目前石斑魚養殖業受到病毒性疾病的威脅,本論文嘗試以電解海水的消毒方式防治石斑神經壞死症病毒 (Grouper nervous necrosis virus, GNNV)以及台灣石斑虹彩病毒(Grouper iridovirus of Taiwan, TGIV)。首先於實驗室自行組裝靜置式電解裝置,評估其效能與特性後,進行兩種石斑魚病毒之不活化,結果對照組紫外線照射與二氧化氯處理水無法將兩種石斑魚病毒不活化,然而電解海水與煅燒牡蠣殼粉處理水能有效將GNNV(107.5TCID50/ml)以及TGIV-2 (104.5 TCID50/ml)病毒力價降至101.0 TCID50/ml以下。在洗卵的應用上,以市售流水式電解裝置產生之電解海水、煅燒牡蠣殼粉與二氧化氯處理水,先進行魚卵之耐受性實驗,發現魚卵對二氧化氯處理水的耐受性較差,超過5ppm處理後之卵孵化率皆在50%以下,幼魚活存率也低於控制組 (90%)與電解海水處理組 (81%);然而,低濃度有效氯 (0.5ppm)之電解海水與煅燒牡蠣殼粉處理水洗卵後之孵化率高於80%,幼魚活存率皆高於控制組,孵化後第四天仍有50%以上,而控制組只有27%。最後以兩種石斑魚病毒進行魚卵攻毒實驗,再由孵化率與孵化後六天幼魚活存率評估效果。結果發現感染的魚卵孵化率會明顯地下降 (50%),幼魚也在第三天之後全數死亡。低有效氯濃度之電解海水處理後能將孵化率提升至70%以上,幼魚活存率可持續到第四天仍有30%以上,若以高濃度 (1ppm)、短時間 (1分鐘)方式處理,孵化率亦有70%以上,幼魚活存率在第四天仍有40%。煅燒牡蠣殼粉處理水能提昇孵化率至90%以上,第四天幼魚活存率超過40%以上。二氧化氯處理水洗卵之孵化率只有50%,幼魚在第三天之後全數死亡。顯示電解海水與煅燒牡蠣殼粉處理水能改善魚卵感染後之孵化率與幼魚活存率。但是電解海水產生之成本較低,操作方便,能處理大量水體,因此未來可以開發電解海水做為新興之消毒方式。
中文摘要 i
英文摘要 iii
目錄 v
圖目錄 vii
表目錄 viii
前言1
文獻整理3
材料與方法17
1、 實驗材料17
2、 研究方法19
2-1、 靜置式電解裝置之組裝與效能分析 19
2-1-1、 靜置式電解裝置之組裝19
2-1-2、 電解海水對魚類細胞毒性之影響21
2-1-3、 三種濾材去除電解海水之有效氯效果比較23
2-2、 靜置式與流水式電解裝置之有效氯生成比較24
2-3、 紫外線照射與三種處理水對兩種石斑魚病毒不活化效果評估24
2-4、 三種處理水洗卵應用於石斑魚病毒防治之效果評估28
2-4-1、 石斑魚卵之耐受性試驗28
2-4-2、 防治魚卵感染石斑魚病毒之有效性試驗31
結果35
1、 靜置式電解裝置之組裝與效能分析35
1-1、 靜置式電解裝置之組裝35
1-2、 電解海水對魚類細胞毒性影響結果 35
1-3、 三種濾材去除電解海水之有效氯結果36
2、 靜置式與流水式電解裝置之有效氯生成比較37
3、 紫外線照射與三種處理水對兩種石斑魚病毒不活化結果37
4、 三種處理水洗卵應用於石斑魚病毒防治之效果評估38
4-1、 石斑魚卵之耐受性試驗結果38
4-2、 防治魚卵感染石斑魚病毒之有效性試驗結果42
討論46
參考文獻51
圖表62

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