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研究生(外文):Hsiao-Cheng Tsai
論文名稱(外文):Stem cell-mediated periodontal ligament regeneration for teeth
外文關鍵詞:Avulsion toothperiodontal stem cellsperiodontal ligament regeneration
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One of the most serious injuries of teeth is avulsion. Two adverse outcomes that are frequently observed after replantation are severe inflammatory resorption and replacement resorption (ankylosis). The former is caused by infection and the latter the result of damaged periodontal ligament (PDL) and cementum. It has been considered that with the presence of healthy cementum and PDL, ankylosis should not occur. Currently, the advocated protocol that can reduce the chance of ankylosis after replantation is the proper storage of the avulsed teeth before the replantation can take place. Various types of storage media that may enhance the survival of PDL have been tested and new media are continuously being investigated. However, there has been no significant breakthrough in the salvage of the avulsed teeth with severely damaged PDL. With the advent of tissue engineering technologies, various types of tissues are being tested for regeneration including PDL. Additionally, recent discovery of human periodontal ligament stem cells (hPDLSCs) provides us a possibility of regenerating PDL of avulsed teeth subjected to prolong exposure to dry or other adverse environments. PDLSCs have been recently shown to enhance the regeneration of periodontal defects in a minipig model. In another application, PDL-like tissue around an engineered bio-root is formed in minipig’s jaws. Based on these premises, we hypothesize that using PDLSCs, PDL can be regenerated in vivo on avulsed teeth.
PDLSCs from teeth of human and their stem cell properties were determined using standard protocols. Ectopic formation of PDL on human teeth was tested by transplanting the root fragment seeded with PDLSCs into the subcutaneous space of immunocompromised mice. After 6 weeks, the samples were resected from mice and subjected to histological and immunohistochemical analyses. The regeneration of PDL was examined using histological and immunohistochemical analyses.

In our results, regeneration of periodontal ligament on the fragments of tooth was found. This study provides the possibility of renew PDL of an avulsed tooth using ex vivo expanded PDLSCs. Further study using animal model for avuled teeth is necessary. This is a first step toward a breakthrough in the management and treatment of avulsed teeth with a more favorable long-term outcome.









ABBREVIATION …………………………………71

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