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研究生:蔡欣宇
研究生(外文):Hsin-yu Tsai
論文名稱:洛神花萃取物對老化促進小鼠抗氧化狀態及延緩衰老之研究
論文名稱(外文):Effect of Hibiscus Sabdariffa L. extracts on antioxidative status and anti-aging in senescence accelerated mice
指導教授:王銘富王銘富引用關係
指導教授(外文):Ming-fu Wang
學位類別:碩士
校院名稱:靜宜大學
系所名稱:食品營養研究所
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
論文頁數:112
中文關鍵詞:老化促進小鼠學習記憶洛神花萃取物抗老化
外文關鍵詞:Senescence-accelerated mouseLearning memoryRoselle extractsAnti-aging
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洛神花Roselle(Hibiscus Sabdariffa L.)具有抗氧化、降低血壓等功能,但能否轉化為抗老化之機能卻尚未獲得證實。實驗以3、6月齡雄性及雌素老化促進小鼠SAMP8 (senescence-accelerated mouse) 為對象,隨機分為對照組一組與實驗組四組,每組10隻,實驗組分別餵食(3.30g/kg BW、6.59g/kg BW、13.19g/kg BW和32.97g /kg BW)等不同劑量之凍乾洛神花萃取物粉末,實驗為期12週。實驗前,進行活動量試驗來測試其行為。實驗期間記錄攝食量、體重變化,並於第11週做老化指數評估,並以單次被動迴避試驗及主動迴避試驗來評估其學習記憶能力。動物犧牲後,採集血液進行血液生化分析,取肝臟檢測抗氧化酵素指標,和過氧化物含量。
結果顯示,各組之間體重、攝食量及活動量並未顯著差異。老化指數評估方面,3、6月齡雄性及雌性小鼠實驗組之老化指數總分皆顯著低於對照組 (p<0.05)。在學習記憶方面,實驗組於單次被動迴避試驗及主動迴避試實驗皆較對照組有顯著之記憶保留及較高之逃避次數 (p<0.05)。在抗氧化方面,洛神花萃取物能提升SAMP8小鼠肝中超氧歧化酶、麩胱苷肽過氧化酶活性、觸酶、總含硫蛋白濃度 (p<0.05);對於過氧化物丙二醛、蛋白質羰基之含量也顯著較對照組低 (p<0.05);降低腦部β-類澱粉蛋白之沉積,相較於對照組有顯著差異 (p<0.05)。綜合以上結果,洛神花萃取物能提升老化促進小鼠體內抗氧化防禦系統之能力減少氧化壓力之傷害、減少腦部β-類澱蛋白沉積及改善小鼠之學習記憶能力,進而推測洛神花萃取物可能具延緩小鼠衰老之功效。
Although the antioxidant and antihypertensive effects of Roselle (Hibiscus Sabdariffa L.) was confirmed, its anti-aging mechanism is still indeterminate. The purpose of this study was to investigate the effects of roselle extracts on learning and memory ability, and antioxidant status in senescence-accelerated mice (SAMP8). Three and six-month-old SAMP8 male and female mice were used in this study. SAMP8 mice were randomly divided into five groups, where each group contained 10 mice, including control group, 3.30 g/kg BW, 6.59 g/kg BW, 13.19 g/kg BW and 32.97 g/kg BW freeze-dried roselle extract powder group. This study period lasted for 12 weeks. Before the experiment , open field activity test was recorded in evaluation for behavior. Besides food intake, the body weight was also measured. One week before scarification, the aging scores were measured, and single-trail passive avoidance test and active shuttle avoidance test were performed independently. The mice were sacrificed to analyze the biochemical parameters of sera. The antioxidant ability and the lipid peroxidation of liver. The results showed that there was no significant difference in the body weight, food intake, and locomotion among each group. The aging score of control groups was significantly higher than experimental groups in 3 and 6-month old mice (p<0.05). Results from ingle-trail passive avoidance test and active shuttle avoidance test showed that experimental groups had significantly better learning memory ability (p<0.05). Activities of antioxidant ability, including SOD, GPx, catalase, and total thiol , in the experimental groups were significantly higher than that of control groups (p<0.05). The MDA and protein carbonyl of liver in the experimental groups were significantly lower than the control groups (p<0.05). Besides, the β-amyloid protein deposition of brain in male and female mice were significantly lower in the experimental groups than control groups (p<0.05) In summary, the supplement of roselle extract may promote the antioxidant defense system, reduce oxidative stress-induced damage,reduce the accumulation of β-amyloid deposition, and improve learning and memory ability. The underlying anti-aging mechanism needs further investigation.
摘 要 I

Abstract II

全目錄 III

表目錄 V

圖目錄 VI


第一章 前言 …………………………………………………… 1
第二章 文獻回顧
第一節 洛神花………………………………………………… 3
第二節 氧化壓力及抗氧化防禦機制………………………… 6
第三節 阿茲海默氏症介紹…………………………………… 12
第四節 老化促進小鼠………………………………………… 16
第三章 材料與方法
第一節 實驗對象……………………………………………… 19
第二節 實驗材料……………………………………………… 20
第三節 實驗方法與步驟……………………………………… 24
第四節 統計與分析…………………………………………… 53
第四章 結果與討論
第一節 體重及攝食量…………………………………………… 54
第二節 活動量……………………………………………… 57
第三節 老化指數……………………………………………… 59
第四節 學習與記憶能力………………………………………… 64
第五節 器官重量……………………………………………… 71
第六節 血液生化學分析………………………………………… 74
第七節 抗氧化酵素分析………………………………………… 77
第八節 過氧化物分析………………………………………… 90
第九節 腦部β-類澱粉蛋白之沉積……………………………… 96
第五章 結論與建議
第一節 結論 ………………………………………………… 104
第二節 建議…………………………………………………… 105
參考文獻 …………………………………………………………… 106
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