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研究生:蕭振宇
研究生(外文):Chen-yu Hsiao
論文名稱:以超臨界二氧化碳萃取喜樹葉中喜樹鹼
論文名稱(外文):Extraction of camptothecin fromCamptotheca acuminate leaves bysupercritical carbon dioxide
指導教授:李順來許孟博
學位類別:碩士
校院名稱:南台科技大學
系所名稱:生物科技系
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
論文頁數:112
中文關鍵詞:喜樹鹼喜樹葉超臨界二氧化碳萃取酒精萃取
外文關鍵詞:camptothecinCamptotheca acuminate leavessupercritical carbon dioxide extractalcohol extraction
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本實驗的主要目的是利用超臨界二氧化碳萃取乾燥喜樹葉中的喜樹鹼(CPT)
成分,並利用高效液相色譜法(HPLC)來分析萃取得到產物中的CPT含量,希望能
建立一套耗能低、低污染、具商業量產價值的分離方法。
萃取方面,改變萃取壓力、萃取溫度、靜態萃取時間、動態萃取流速、修飾
劑添加比例各種變因,探討萃取效果最佳的操作條件,並與傳統酒精萃取喜樹鹼
法CPT含量做比較。
目前研究結果顯示,在無添加修飾劑情況下,以萃取壓力:500 Bar、萃取溫
度:60℃、靜態萃取時間:40 mins、動態萃取時間:10 mins、動態萃取流速:8L/min,
有最高喜樹鹼萃取率:0.0263 mg CPT/g dry weight,為乙醇萃取的喜樹鹼萃取率
(1.086 mg CPT/g dry weight)的2.42%。
在添加60%乙醇為修飾劑情況下,以萃取壓力:500 Bar、萃取溫度:60℃、靜態
萃取時間:60 mins、動態萃取時間:10 mins、動態萃取流速:8L/min,有最高喜樹鹼
萃取率:0.2173 mg CPT/g dry weight,為乙醇萃取的喜樹鹼萃取率(1.086 mg CPT/g
dry weight)的20%。
添加了修飾劑後,對於喜樹鹼萃取率有顯著提升(無修飾劑最高喜樹鹼萃取率
為0.0263 mg CPT/g dry weight,60%乙醇當修飾劑最高喜樹鹼萃取率為0.2173 mg
CPT/g dry weight,提升了726%),在SCF產物重方面也有明顯助益(無修飾劑最高
SCF產物重為0.1 g,60%乙醇當修飾劑最高SCF產物重為0.2562 g,提升了156%),
但對於喜樹鹼純度則提升幅度較小(無修飾劑最高喜樹鹼純度為1.02%,60%乙醇當
修飾劑最高喜樹鹼純度為1.32%g,提升了29%)
The main task of this experiment is to extract camptothecin(CPT) from dried
Camptotheca acuminate leaves by using supercritical carbon dioxide.
High-performance liquid chromatography (HPLC) was used to analyze the
camptothecin content of extraction products. Our goal is to establish a separation
method with low energy consumption, low pollution and commercial value.
The extraction pressure, extraction temperature, static extraction time, dynamic
extraction flow rate, the proportion of modifier were varied to find the best conditions,
and the results were compared with the traditional alcohol extraction method.
The present study showed that in the absence of modifier condition, extract
pressure: 500 Bar, extraction temperature: 60 ℃, static extraction time: 40 mins,
dynamic extraction time: 10 mins, dynamic extraction flow rate: 8L/min, has the highest
CPT extraction rate: 0.0263 mg CPT / g dry weight, the CPT extraction ratio compared
to the alcohol extraction method (1.086 mg CPT / g dry weight) is 2.42%.
Adding 60% ethanol as modifier condition, extract pressure: 500 Bar, extraction
temperature: 60 ℃, static extraction time: 60 mins, dynamic extraction time: 10 mins,
dynamic extraction flow rate: 8L/min, has the highest CPT extraction rate: 0.2173 mg
CPT / g dry weight, the CPT extraction ratio compared to the alcohol extraction method
(1.086 mg CPT / g dry weight) is 20%.
With the addition of modifier, the CPT extraction rate can increased as high as
726%(the maximum extraction rate of 0.0263 mg CPT / g dry weight without modifier,
0.2173 mg CPT / g dry weight with 60% ethanol addition). The weight extraction
increased as high as 156%(the maximum SCF weight is 0.1 g without modifier, 0.2562
g with 60% ethanol addition). CPT purity of extract, however, was raised to a lesser
extent (the CPT highest purity is 1.02% without modifier, the highest CPT purity is
1.32% with 60% ethanol addition, increase by 29%)
摘要 iv
英文摘要v
誌謝vi
目次vii
表目錄x
圖目錄xi
第一章緒論 1
1.1 前言1
1.2 研究動機和實驗目的1
第二章文獻回顧 3
2.1 喜樹3
2.1.1 喜樹的地理分佈與繁殖現況3
2.1.2 喜樹生物活性成分3
2.2 喜樹鹼介紹4
2.2.1 喜樹鹼的研究及應用4
2.2.2 喜樹鹼抗癌作用機轉4
2.2.3 喜樹鹼物化特性7
2.2.4 喜樹鹼的臨床應用8
2.3 喜樹鹼萃取方式8
2.3.1 鹼水萃取8
2.3.2 酸水萃取9
2.3.3 酒精萃取9
2.3.4 管柱層析與分離9
2.3.5 樹脂吸附9
2.3.6 超臨界二氧化碳萃取10
2.4 超臨界二氧化碳10
2.4.1 超臨界流體的歷史與發展10
2.4.2 超臨界流體的定義11
2.4.3 超臨界流體的性質14
2.4.4 超臨界流體萃取的基本原理19
2.4.5 影響超臨界流體萃取之因素21
2.4.6 超臨界二氧化碳之特性21
2.4.7 修飾劑的添加26
2.4.8 超臨界二氧化碳萃取在食品工業上的應用27
2.4.9 超臨界二氧化碳萃取在生物分離技術的應用32
2.4.10 超臨界流體在材料科學領域的應用33
2.4.11 超臨界流體在化學反應之應用33
2.4.12 超臨界流體在矽半導體工業之應用34
2.4.13 超臨界流體在染色技術的應用34
第三章材料與方法 35
3.1 實驗材料與實驗儀器35
3.1.1 實驗材料35
3.1.2 實驗藥品35
3.1.3 實驗設備35
3.2 實驗方法38
3.2.1 實驗流程38
3.2.2 實驗步驟39
3.2.3 HPLC 分析方法40
3.2.4 UV 偵測波長的選擇40
3.2.5 HPLC 移動相條件的選擇41
3.2.6 標準曲線的繪製41
第四章結果與討論 42
4.1 喜樹鹼標準品檢量線的繪製42
4.2 喜樹鹼溶液之HPLC 層析圖與分析含量44
4.3 乾燥喜樹葉酒精萃取喜樹鹼含量48
4.4 超臨界二氧化碳萃取喜樹鹼50
4.4.1 無添加修飾劑情況下,萃取壓力的探討50
4.4.2 無添加修飾劑情況下,萃取溫度的探討54
4.4.3 無添加修飾劑情況下,靜態萃取時間的探討58
4.4.4 無添加修飾劑情況下,動態萃取流速的探討61
4.4.5 有添加修飾劑情況下,添加乙醇比例的探討66
4.4.6 有添加修飾劑情況下,萃取壓力的探討70
4.4.7 有添加修飾劑情況下,萃取溫度的探討74
4.4.8 有添加修飾劑情況下,靜態萃取時間的探討77
4.4.9 有添加修飾劑情況下,動態萃取流速的探討80
第五章結論 85
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