研究目的欲瞭解補充達瑪烷次苷對耗竭運動後大鼠骨骼肌血管新生的影響，並進一步探討是否會改變耗竭運動後所產生的發炎反應。方法：100隻Sprague Dawley大鼠以體重平均分配方式分為：控制組、達碼烷次苷Dammarance Oligo-Sapogenin (DS) 組 (0.1 mg/kg BW)、DS20組 (20mg/kg BW)、DS60 (60mg/kg BW) 組、DS120組 (120mg/kg BW)，又分成無運動與運動兩小組作為對照，各組n=10。以餵管方式介入不同劑量之達瑪烷次苷十週，運動組給予單次游泳耗竭運動後，立即取出肌肉組織。結果：游泳耗竭運動後Placebo組蹠肌中VEGF、PGC-1α、IL-6 mRNA皆呈顯著增加。而在補充達瑪烷次苷的組別耗竭運動後與Placebo exercise相較發現蹠肌中P-AMPK蛋白表現量在DS20組顯著增加 (p<.05)，PGC-1α mRNA在DS20、DS60、DS120組則有下降的趨勢，並且蛋白表現量在DS60、DS120組是顯著下降的 (p<.05)。血管生長因子的部份，在耗竭運動後VEGF蛋白也與PGC-1α同樣在DS20、DS60、DS120組有下降的趨勢，Ang-1、Ang-2 mRNA、蛋白表現則沒有差異。此外，補充達瑪烷次苷的組別在耗竭運動後蹠肌與比目魚肌中IL-6 mRNA發炎程度是受到控制的。結論：補充十週達瑪烷次苷能減少運動誘導PGC-1α和VEGF增加的壓力反應，並有助於減緩耗竭運動所產生的發炎指標IL-6反應。肌肉微血管密度並未因長期服用達瑪烷次苷而影響。

Purpose: The present study was aimed to investigate the effect of Dammarance Oligo-Sapogenin (DS) on angiogenesis and inflammatory markers after exhaustive exercise in rat skeletal muscle. Methods: Sprague Dawley rats (n=100) were equally assigned into 5 groups: placebo, DS, DS20, DS60 and DS120. Respective doses of bioactive compound DS (0.1, 20, 60 and 120 mg/kg body weight) were orally administered to DS groups for 10-week. Ten rats from each group performed a single bout of exhaustive swimming exercise and then muscle tissue was excised immediately after exercise. Results: Plantaris muscle VEGF, PGC-1α and IL-6 mRNA expression were significantly (p<.05) increased after exhaustive exercise in placebo group. phospho-AMPK protein level in DS20 was significantly (p<.05) increased after exhaustive exercise compare to placebo exercised rats. PGC-1α mRNA expression showed decreased trend in DS20, DS60, DS120 groups after exercise compared to placebo exercise group, while PGC-1α protein was significantly (p<.05) decreased only in DS60 and DS120 groups. VEGF protein level was decreased in DS20, DS60 and DS120 groups after exhaustive exercise. We observed no change in Angiopoeitins (Ang-1 and Ang-2) mRNA and protein levels after exhaustive exercise in DS pre-treated rats compare to placebo exercise in both muscle types. However, increased IL-6 mRNA expression after exhaustive exercise was controlled in DS20, DS60 and DS120 pre-treated rats in both muscle types. Conclusions: This study results conclude that Dammarance Oligo-Sapogenin supplementation can attenuate stress response in PGC-1α and VEGF by exhaustive exercise. Furthermore, increased inflammatory marker IL-6 after exhaustive exercise was controlled in the DS pre-treated rats.

中文摘要 IV
Abstract VI
謝誌 VIII
目錄 IX
表目錄 XII
圖目錄 XII
第壹章 緒 論 1
第一節、研究背景 1
第二節、研究目的 3
第三節、研究的重要性 3
第四節、名詞操作型定義 3
第五節、研究範圍及限制 4
第貳章 相關文獻探討 5
第一節、血管新生及其調控 5
第二節、運動與血管新生 7
第三節、運動與發炎反應 9
第四節、人參萃取物─達瑪烷次苷 10
第參章 研究方法與研究步驟 13
第一節、動物的選取與照料 13
第二節、實驗設計 13
一、實驗流程 13
二、各組處置 14
三、運動方式 15
第三節、實驗材料與分析方法 15
一、實驗麻醉 15
二、肌肉組織RNA之純化 15
三、cDNA之反轉錄 15
四、基因表現量分析/Real-Time PCR 16
五、蛋白質分析/西方點墨法Western Blotting Analysis 16
六、檸檬酸合成酶活性 17
七、組織化學染色法 18
八、統計分析 18
第肆章 結果 19
一、補充達瑪烷次苷後的動物PGC-1α、血管新生因子以及發炎指標mRNA之分析 19
二、補充達瑪烷次苷的動物在耗竭運動後PGC-1α、血管新生因子以及發炎指標mRNA之分析 19
三、補充達瑪烷次苷後的動物AMPK、PGC-1α以及血管新生因子蛋白之分析 20
四、補充達瑪烷次苷的動物在耗竭運動後AMPK、PGC-1α以及血管新生因子蛋白之分析 21
五、補充達瑪烷次苷的動物在耗竭運動後檸檬酸合成酶活性之分析 22
六、AP (Alkaline Phosphatase) 組織化學染色 22
第伍章 討論 23
第一節、達瑪烷次苷對耗竭運動後AMPK的影響 23
第二節、達瑪烷次苷對耗竭運動後PGC-1α以及血管新生指標的影響 24
第三節、達瑪烷次苷對耗竭運動後檸檬酸合成酶活性的影響 26
第四節、達瑪烷次苷對耗竭運動後發炎指標的影響 27
第五節、達瑪烷次苷對肌肉中微血管密度的影響 28
第陸章 結論 29
參考文獻 30
圖與表說明 36
附錄 69

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