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研究生(外文):Chia-Hui Chang
論文名稱(外文):Association of SARS-CoV Membrane Protein with Nucleocapsid Protein
指導教授(外文):Chin -Tien Wang
外文關鍵詞:SARS-CoVMembrane proteinNucleocapsidInteraction
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SARS冠狀病毒(SARS-CoV)包含四個結構蛋白:膜蛋白(M)、核鞘蛋白(N)、套膜蛋白(E)和棘蛋白(S)。許多研究指出,M和N共同表現時會藉由M的C端與N產生交互作用形成類病毒粒子(VLPs)。而我們之前研究發現,單獨表現M就能組裝形成membrane-enveloped vesicles釋出到細胞外。本篇研究欲探討M序列中M-M和M-N交互作用的位置。經由定位突變(site-directed mutagenesis)建構突變的M質體,將突變的M質體單獨表現或與N共同表現。觀察VLPs釋出之能力;經由pull down assay分析M-M以及M-N交互作用之胺基酸序列,更進一步探討定位突變後的M的聚合程度並觀察細胞內分佈的情形。結果顯示,M的N端(HA-M)不影響M的組裝和M-N形成VLP,然而,在M的C端末接上FLAG以及L218L219取代為Ala明顯地影響與N的交互作用。M失去N-linked醣基化(N4Q),不影響VLPs的產生;M定位突變質體W19A、W91A、Y94A、F95A以及F95L皆顯著地降低VLPs的釋出。進一步探討發現,釋出能力有缺陷的M不影響M-M和M-N交互作用之能力,釋出能力有缺陷的M雖然可產生聚合作用,但部分的能力遭受影響。觀察M在細胞內分佈位置,結果發現破壞M醣化作用以及釋出能力有缺陷的M在細胞內分佈位置與原生型相近,分佈於細胞核周圍和細胞膜上。因此,我們認為釋出能力有缺陷的M雖然不會影響M-M、M-N交互作用能力以及細胞內分佈的位置,但定位突變的位置對於M自我組裝(self-assembly)的能力極為重要。
Severe acute respiratory syndrome coronavirus (SARS-CoV) encodes four structural proteins i.e. membrane (M), nucleocapsid (N), envelope (E) and spike (S). Coronavirus M is necessary for virus-like particles (VLPs) formation. A number of studies have shown that co-expression of M and N can generate substantial amoutns of VLPs and that the M carboxyl-terminal region is important for M-N interaction. Our previous study, however, demonsated that the M can self-assemble and release from cells. We aim to map the domain functionally involved in M-M self-association and M-N interaction. A panel of M mutatns was constructed by site-directed mutagenesis, and each of the resutant M mutants was transiently expressed or co-expressed with N. The release efficiency of VLPs was determined by Western blot. The M-M association and M-N interaction domain was further determined by pull-down assay. The oligomerization and localization of secretion-defective M mutants were analyzed. The data showed that HA tagged at the amino-terminus of M does not significantly affect M self-assembly or M plus N VLP formation. However, substitutions or FLAG tagged at the very carboxyl-terminus of M markedly impaired M-N interaction. Blocking glycosylation of M (N4Q) has no major impacts on M self-assembly and VLP release. Alanine substitution in either W19 or W91 significantly reduced VLP production. The release efficiency of VLPs was also markedly reduced when Y94 and F95 were replaced with alanine or leucine residues. However, the secretion-defective M can release into medium when co-expressed with wild-type M. In addition, GST pull-down assays suggest that these secretion-defective M mutants can interact with N protein. The localization of glycosylation-deficient M and secretion-defective M was similar to wild-type M in that they localized in the plasma membrane and perinuclear areas. Velocity sedimentation analysis suggests that multimerization of most secretion-defective was impaired to a certain extent. These results suggest that although the substitution mutations in M have no detrimental effects on M-M or M-N interaction but they significantly affect M self-assembly and release.
目 錄
誌謝……………………………………………………………………. i
中文摘要……………………………………………………………… ii
英文摘要……………………………………………………………… iv
目錄………………………………………………………………… vi
第一章 序論
SARS冠狀病毒的發現及起源……………………………………… 1
SARS冠狀病毒的分類學…………………………………………… 3
SARS冠狀病毒的基因結構………………………………………… 4
冠狀病毒生活史…………………………………………………… 5
SARS冠狀病毒結構及其結構蛋白………………………………… 5
實驗動機與設計…………………………………………………… 12
第二章 材料與方法
研究材料…………………………………………………………… 13
實驗方法…………………………………………………………… 17
第三章 實驗結果…………………………………………………… 28
第四章 討論………………………………………………………… 36
參考文獻……………………………………………………………… 43
圖表說明……………………………………………………………… 54
附錄………………………………………………………………… 70

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