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研究生:徐維駿
研究生(外文):HSU,WEI-CHUN
論文名稱:射出成型光柵耦合生物感測器之研究與應用
指導教授:謝文馨謝文馨引用關係
口試委員:周禮君馮國華謝文馨
口試日期:2011-07-05
學位類別:碩士
校院名稱:國立中正大學
系所名稱:機械工程學系暨研究所
學門:工程學門
學類:機械工程學類
論文種類:學術論文
論文出版年:2011
畢業學年度:99
語文別:中文
論文頁數:90
中文關鍵詞:光柵生物感測器
外文關鍵詞:gratingbiosensor
相關次數:
  • 被引用被引用:1
  • 點閱點閱:232
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  • 下載下載:25
  • 收藏至我的研究室書目清單書目收藏:0
本研究目的在於設計並製造一結合光柵與平面波導之生物感測器。該感測器利用光柵耦合將雷射光耦合進平面波導,當抗體與抗原在波導表面鍵結時,波導表面之有效折射率將會改變,導致耦合進波導之能量改變,利用此偵測機制檢測生物分子,得到高靈敏度之偵測極限。
本研究從晶片製作與光學系統設置確認晶片偵測可行性,並使用點平均方式降低雜訊得到最佳晶片檢測解析度5.13×10-6 RIU,且確認其檢測結果擁有良好重覆性。由研究結果得知其晶片偵測機制主要乃是因為光柵表面介質改變而導致耦合效率隨之改變。
隨後而使用物理性吸附方式針對Rabbit IgG進行生化檢測得到偵測極限為4.76×10-7 g/ml。以及進行化學修飾針對Anti-DNP進行檢測得到偵測極限為7.92×10-8 g/ml,並得到其線性檢測範圍約為兩個量級。
摘要 I
目錄 II
表目錄 V
圖目錄 VII
第一章 緒論 1
1-1前言 1
1-2光波導層 2
1-3射出成型 4
1-4文獻回顧 4
1-5研究動機與目的 7
1-6研究貢獻 8
第二章 研究方法 15
2-1前言 15
2-2-1藥品 16
2-2-2儀器設備 17
2-3光柵耦合生物感測晶片製作 19
2-3-1光柵模仁製作 19
2-3-2射出成型光柵晶片 21
2-3-3二氧化鈦波導層製作 22
2-3-4晶片槽道設計 22
2-4檢測系統及數據分析 23
2-4-1光學檢測系統及架設 23
2-4-2檢測解析度計算 23
2-4-3偵測極限與定量極限計算 24
2-4-4數位濾波降低雜訊 25
2-5射出成型光柵耦合生物晶片之特性量測 26
2-5-1耦合能量與光柵相對位置 26
2-5-2晶片檢測所需樣本量 27
2-5-3 折射率檢測解析度檢測 28
2-6射出成型光柵耦合生物晶片之生化檢測 29
2-6-1物理性吸附Anti-rabbit IgG 29
2-6-2 Rabbit IgG檢測 31
2-6-3 DNP分子修飾 32
2-6-4 Anti-DNP檢測 33
2-6-5非特異性吸附測試 34
第三章 結果與討論 47
3-1晶片輪廓 47
3-2光學訊號測試 47
3-2-1光學系統長時間穩定測試 47
3-2-2數位濾波雜訊處理結果 48
3-3檢測晶片之特性量測結果 48
3-3-1光柵與耦合能量之相對位置 48
3-3-2晶片檢測解析度及再現性 49
3-3-3檢測系統所需樣本量 50
3-4生化檢測實驗結果 51
3-4-1物理吸附生物分子 51
3-4-2生化檢測(anti-rabbit IgG/rabbit IgG) 51
3-4-3生化檢測(DNP/anti-DNP) 51
3-4-4非特異性吸附實驗結果 54
第四章 結論與未來研究方向 74
4-1 結論 74
4-2未來研究方向 75
參考文獻 76

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