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研究生:陳光宏
研究生(外文):Guang-hung Chen
論文名稱:葡萄糖與木糖共醱酵產製酒精
論文名稱(外文):Ethanol production with co-fermentation of glucose and xylose
指導教授:林屏杰李國興李國興引用關係
指導教授(外文):Ping-Jei LinKuo-Shing Lee
學位類別:碩士
校院名稱:逢甲大學
系所名稱:化學工程學所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2011
畢業學年度:99
語文別:中文
論文頁數:92
中文關鍵詞:共醱酵酒精醱酵
外文關鍵詞:co-fermentationethanol fermentationZymomonas mobilisPichia stipitis
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在第二代纖維素酒精醱酵製程中,如需有減少建廠成本以及提高產率,應該積極開發共醱酵製成,而Z. mobilis與P. stipitis為目前主要研究的酒精醱酵菌,有鑒於此本研究想將兩者結合,進行連續共醱酵製程之開發研究。
本研究中可以發現Z. mobilis的最佳操作條件為葡萄糖濃度140 g/L,溫度在35℃下會有較佳的酒精醱酵表現;P. stipitis的最佳操作條件為木糖濃度40 g/L,溫度30℃以及培養箱轉速在200 rpm,空氣曝氣速率在75 vvm下會有較佳的表現。
Z. mobilis經固定化進行連續酒精醱酵,可以得到平均酒精濃度67 g/L且基質利用率為96 %,而最大酒精生產速率為16.75 g/L/h,P. stipitis再饋料批次操作下,平均酒精濃度達13.3 g/L而最大酒精生產速率為0.37 g/L/h。
In the cellulosic ethanol fermentation process, if plant for a decrease
in costs and improve productivity, this research of co-fermentation should
be made active, and Z mobilis and P. stipitis for the current main
research fermentation of ethanol, this study is to combine the
two,development researching of continuous co-fermentation process.
The study found that Z. mobilis can be the best operating conditio
ns for the glucose concentration 140 g/L, temperature at 35℃ will be b
etter fermentation performance; P. stipitis the best operating condition
s for the xylose concentration of 40 g /L, temperature 30 ℃ and the in
cubator speed at 200 rpm, aeration rate of 75 vvm air will be a better
performance.
Z. mobilis immobilized continuous fermentation, ethanol can be an
average of 67 g/L and substrate utilization of 96%, while the ethanol
productivity of 16.75 g/L/h, P. stipitis at the fed-batch operation, the
average ethanol concentration of 13.3 g/L while the ethanol productivit
y of 0.37 g/L/h.
中文摘要........................................................................................ i
英文摘要................................................................................................... ii
目錄...........................................................................................................iii
圖目錄.................................................................................................... viii
表目錄........................................................................................................xi
第一章 緒論..............................................................................................1
1.1 前言.....................................................................................................1
1.2 研究動機與目的.................................................................................2
第二章 文獻回顧......................................................................................5
2.1 再生能源的發展.................................................................................5
2.2 酒精醱酵菌.........................................................................................6
2.2.1 六碳糖醱酵微生物...................................................................6
2.2.1.1 酒精醱酵微生物比較.....................................................6
2.2.1.2 Zymomonas mobilis的特性..............................................9
2.2.1.3 Zymomonas mobilis的代謝特性....................................11
2.2.2 五碳糖醱酵微生物.................................................................11
2.2.2.1 酒精醱酵微生物比較....................................................11
2.2.2.2 Pichia stipitis的特性......................................................15
2.2.2.3 Pichia stipitis的代謝特性..............................................17
2.3 微生物固定化技術...........................................................................17
2.3.1 細胞固定化定義與優勢.........................................................17
2.3.2 細胞固定化的方法.................................................................18
2.3.3 包埋法.....................................................................................20
2.4 反應曲面法(RSM)實驗設計...........................................................21
第三章 材料與方法................................................................................23
3.1 菌株來源...........................................................................................23
3.2 設備與藥品.......................................................................................23
3.2.1 設備儀器.................................................................................23
3.2.2 生物反應器構形.....................................................................24
3.2.3實驗藥品..................................................................................26
3.3 培養基配方.......................................................................................26
3.3.1 Z. mobilis培養基......................................................................26
3.3.2 P. stipitis培養基.......................................................................27
3.3.3 磷酸鹽緩衝溶液.....................................................................27
3.3.4 固定化細胞保存液.................................................................28
3.4 實驗方法...........................................................................................28
3.4.1 菌種預培養.............................................................................28
3.4.1.1 Z. mobilis預培養............................................................28
3.4.1.2 P. stipitis預培養.............................................................28
3.4.2 分析方法.................................................................................29
3.4.2.1 葡萄糖與乙醇濃度測定...............................................29
3.4.2.2 菌體濃度測定...............................................................29
3.4.2.3 菌體乾重測定...............................................................29
3.4.2.4 修正型Gompertz Model 模試探討..............................30
3.4.2.5 酒精生產速率...............................................................31
3.4.3 細胞固定化方法.....................................................................32
3.4.4 最適培養條件探討.................................................................32
3.4.4.1 Z. mobilis最適培養條件探討........................................32
3.4.4.1.1 不同碳源濃度之酒精醱酵批次實驗..................32
3.4.4.1.2 不同溫度之酒精醱酵批次實驗..........................32
3.4.4.2 P. stipitis最適培養條件探討.........................................32
3.4.4.2.1 不同碳源濃度之酒精醱酵批次實驗..................32
3.4.4.2.2 不同溫度之酒精醱酵批次實驗..........................32
3.4.4.2.3 不同pH之酒精醱酵批次實驗............................33
3.4.4.2.4 不同轉速之酒精醱酵批次實驗..........................33
3.4.4.3 共醱酵進行酒精醱酵批次實驗...................................33
3.4.5 連續操作探討.........................................................................33
3.4.5.1 以連續操作探討Z. mobilis酒精醱酵..........................33
3.4.5.2 以連續操作探討P. stipitis酒精醱酵............................34
3.4.5.3 以連續操作探討酒精共醱酵.......................................34
第四章 結果與討論................................................................................35
4.1 最適培養條件探討...........................................................................35
4.1.1 Z. mobilis最適培養條件探討..................................................35
4.1.1.1 不同碳源濃度之酒精醱酵批次實驗...........................35
4.1.1.2 不同溫度之酒精醱酵批次實驗...................................38
4.1.2 P. stipitis最適培養條件探討...................................................41
4.1.2.1 不同碳源濃度之酒精醱酵批次實驗...........................41
4.1.2.2 不同溫度之酒精醱酵批次實驗...................................44
4.1.2.3 不同pH之酒精醱酵批次實驗......................................47
4.1.2.4 不同轉速之酒精醱酵批次實驗...................................50
4.1.2.5 以最佳條件進行酒精醱酵批次實驗...........................53
4.1.2.6 以不同曝氣速率進行酒精醱酵批次實驗...................56
4.2 連續操作探討...................................................................................59
4.2.1 以連續操作探討Z. mobilis之酒精醱酵................................59
4.2.2 以饋料批次操作探討P. stipitis轉化木糖進行酒精醱酵......68
第五章 結論與建議................................................................................70
5.1 結論...........................................................................................70
5.2 建議............................................................................................71參考文獻..................................................................................................72
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