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研究生:黃筱惠
研究生(外文):Shiau-Huei Huang
論文名稱:大花咸豐草萃取物於腸道上皮細胞 Caco-2 之生物利用率及前脂肪細胞 3T3-L1抑制分化作用
論文名稱(外文):Bioavailability in Caco-2 Cells and Anti-Adipogenic Effect on 3T3-L1 Cells Exerted By The Extracts of Bidens pilosa L. var. radiata Sch. Bip. Compositae
指導教授:喬長誠喬長誠引用關係彭耀寰彭耀寰引用關係
指導教授(外文):Charng-Chermg Chyau
學位類別:碩士
校院名稱:弘光科技大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
畢業學年度:99
語文別:中文
論文頁數:138
中文關鍵詞:大花咸豐草多酚聚炔類生物利用率Caco-2細胞3T3– L1細胞
外文關鍵詞:Bidens pilosa L. var. radiataPolyphenolicsPolyacetylenesBioavailabilityCaco-23T3-L1
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咸豐草為民間傳統用藥之ㄧ,具有抗發炎、止血及抗菌的特性。在動物
實驗中,大花咸豐草萃取物已被證實具有抗癌的活性,其萃取物亦被報導具
有保護正常人體紅細胞免受氧化損傷之功能,唯相關萃取物成分中,其活性
之表現如何,仍尚未有系統性之研究。本研究探討大花咸豐草甲醇萃取物中
乙酸乙酯區分物於 Caco-2 細胞和 3T3- L1 細胞中的生物利用率和抑制脂肪形成的作用。

實驗分三個部份,第一部分為探討溶劑之最佳萃取條件,以常用之溶劑,
包括水、酒精、正己烷、甲醇及正丁醇比較結果,以甲醇具最佳之效果,並
以40:1 ( V/W )為最適比例,且以超音波輔助萃取時間60分鐘,具最高之萃取率,由甲醇萃取物進一步區分獲得之乙酸乙酯區分物( ME/EA )中,總酚類化合物和黃酮類化合物含量較高( 752 ± 7.53 mg/g 和469 ± 0.97mg/g )並可增加3T3-L1中之超氧陰離子歧化酶( SOD )、觸酶( CAT )和麩胱苷肽轉移酶( GSH ) 活性,且具較高 DPPH 清除活性和還原力。第二部分為探討甲醇萃取物及乙酸乙酯區分物在抑制 3T3-L1 脂肪細胞形成之能力,包括細胞存活率、乳酸脫氫酶 ( LDH ) 活性分析、細胞週期分析及Oil-Red-O染色分析,於Oil-Red-O染色分析中,結果顯示 ME/EA 區分物較甲醇萃取物具有較佳之活性,在濃度範為( 2µg/mL-20µg/mL)呈劑量相關性。第三部分為,利用 Caco-2 細胞作為吸收利用率試驗,探討萃取物之生物利用率,結果顯示在乙酸乙酯區分物中主要之六個成份皆可在腸道中通透,其中以酚類化合物較聚炔類化合物具有較佳之通透速率。

由上述實驗之結果,發現大花咸豐草之乙酸乙酯區分物具有最佳之生物
活性,其中已鑑定之主要組成為三種酚類化合物與三種聚炔類化合物,可作
為大花咸豐草萃取物產品活性成分指標之參考。

Bidens pilosa L. var. radiata is a traditional folkloric medicine, reported to exhibit anti-inflammatory, heamostatic, and antibacterial bioactivities. Its
anti-tumorigenic effect has been verified in animal model. Moreover, the extract of Bidens pilosa is effective against the antioxidative stress in human normal erythrocytes. The absorption behavior of the extract in vitro is still lacking. We performed this study with Caco-2 and 3T3-L1 cell lines using the methanolic/ethyl acetate extract (ME/EA) of Bidens pilosa L. var. radiata investigating its bioavailability and anti-adipogenetic effect.
This research was conducted in three parts:
Part I:
In Part I, the primary optimum condition for solvent extraction was determined. The solvents water, ethanol, n-hexane, methanol, and butanol were used to compare the extractability. The efficiency was enhanced by microwave-assisted extraction for 60 min, as for the methanol, a ratio methanol to the desiccated herb was found optimum at 40:1 (v/w). The primary methanolic extract, when further
fractionated with ethyl acetate, the extract ME/EA was obtained, which was characteristically to contain the highest total polyphenolics and flavonoids, reaching 752 ± 7.53 mg/g and 469 ± 0.97 mg/g, respectively. These constituents showed strong enhancing effect on the activity of cytosolic SOD, GST, and GSH, and at the same time it revealed more potent DPPH scavenging capability and
anti-oxidative reducing power.
Part II:
In Part II, the ME/EA fraction was tested for its anti-adipogenic bioactivity in 3T3-L1 cell line. We compared the cell viability and performed cell cycle analysis,
LDH assay, and Oil-Red-O staining. The fraction ME/EA showed rather promising efficiency and better dose-responsive effect in this regard.
Part III:
In the Part III, we identified the presence of at least six major components in the ME/EA fraction. In which we identified three major polyphenolics and polyacetylides. We examined the cytopermeability through Caco-2 cell line, taking it to be comparable to bioavailability. All six constituents showed acceptable permeability through the cell membrane, implicating their good intestinal
absorbability. The polyphenolics showed better transportability, being more readily absorbed than the polyacetylides.
Conclusively, the fraction ME/EA exhibits the most promising bioactivity. The three polyphenolics together with the polyacetylides are suggestible to serve as the
process bioactivity markers mostly feasible to facilitating the isolation and purification of the bioactive crude extract from Bidens pilosa L. var. radiata.
目錄. . . . . . . . . . . . . . . . . . . . ....... . . . I
圖目錄. . . . . . . . . . . . . . . . . . . . . . . ... III
表目錄. . . . . .. . . . . . . . . . . . . . . . . . . . .V
中文摘要. . . . . . . .. . . . . . . . . . . . . . .. . .VII
英文摘要. . . . . . . . . . . . . . . . . . . . . ... .VIII
第一章 序論. . . . . . . . . . . . . . . . . . . . . . . .1
第二章 文獻整理. . . . . . . . . . . . . . . . . . . . . .3
第三章 研究目的與實驗架構..................................21
一、研究目的..............................................22
二、實驗架構..............................................22
第四章 材料與方法.........................................23
第一節、材料來源...........................................23
(一)、大花咸豐草樣品.....................................23
(二)、實驗細胞株.........................................23
(三)、化學藥品與試劑.....................................23
(四)、儀器設備................ ......... .... ...........25
第二節、實驗方法...........................................26
(一)基源鑑定............................................26
(二)最適萃取條件.........................................29
(三)萃取物製備...........................................31
(四)酚類化合物含量.......................................32
(五)抗氧化性檢測.........................................33
(六)HPLC 成分分析.......................................35
(七)細胞培養.......................................................39
(八)細胞存活率測定.......................................................41
(九)LDH 活性測定........................................41
(十)細胞週期分析.........................................42
(十一)細胞內抗氧化酵素活性之分析...........................42
(十二)脂肪細胞分化.......................................46
(十三)Oil-Red-O 染色法..................................46
(十四)三酸甘油酯含量測定..................................47
(十五)Transwell insert 培養法..........................48
(十六)統計分析..........................................49
第五章 實驗結果..........................................50
第一節、咸豐草之親緣鑑定分析................................50
第二節、最適萃取條件.......................................53
第三節、咸豐草之產率與分類化合物之組成.......................82
第四節、咸豐草之抗氧化能力分析..............................85
第五節、細胞存活率實驗(MTT)...............................93
第六節、LDH 活性測定.......................................97
第七節、細胞週期分析.......................................99
第八節、細胞內抗氧化酵素之分析.............................105
第九節、Oil-Red-O 染色分析................................109
第十節、大花咸豐草萃取物對三酸甘油酯堆積之影響...............114
第十一節、單層膜完整性測試.................................115
第十二節、單層膜通透實驗...................................116
第六章 討論.............................................118
第七章 結論.............................................123
第八章 參考文獻..........................................124
附錄一、序列比對結果......................................135

台灣野生植物資料庫http://plant.tesri.gov.tw/plant/

彭鏡毅。1976。台灣菊科植物的系統分類與染色體之研究。國立台灣大學森
林研究所碩士論文。

鄧書麟、呂福原、何坤益。2003。台灣產鬼針屬植物分類之研究。中華林學
季刊。36(4):318-326。

趙愛華,趙勤實,彭麗豔,張積霞,林中文,孫漢董。2004。鬼針草中一個
新的查耳酮甙。雲南植物研究,26(1):121-126

王啟賢。2001。益生菌 L. GG吸附在Caco-2細胞上對抗生素及生物黃酮通透
的影響。大同大學生物工程研究所碩士論文。

宋姿瑩。2007。迷迭香葉超臨界流體萃取物之抗氧化及抗發炎活性。東海大
學食品科學研究所碩士論文。

李昉。2007。野生植物鬼针草營養成分的研究。山東化工。36 ( 3 ):37-38

李淵菘。2008。紫蘇子及其脫脂粕萃取物之抗氧化與抗發炎作用。東海大學
食品科學研究所碩士論文。

Abajo, C.; Boffill, M. A.; Campo, J.; Méndez, M.A.; González, Y.; Mitjans, M.; Vinardell, M. P. In vitro study of the antioxidant and immunomodulatory activity of aqueous infusion of Bidens pilosa. J. Ethnopharmacol. 2004, 93(2), 319-323.

Arnao, M. B.; Cano, A.; Acosta, M. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chem. 2001, 73, 239-244.

Bairwa, K.; Kumar, R.; Sharma R. J.; Roy R. K. An updated review on Bidens Pilosa L. Der Pharma Chemica. 2010, 2(3), 325-337.


Benhura, M. A. N.; Chitsiku, I. C. The extractable β-carotene content of Guku (Bidens pilosa) leaves after cooking, drying and storage. Int. J. Food Sci. Technol. 1997, 32, 495-500.

Bohman, B. A.; Stuessy, T. F. Flavonoids of the Sunflower Family (Asteraceae); Springer-Verlag: Wien, Austria. 2001; Chapter 11, 260-263.

Brown, G. D. The biosynthesis of artemisinin (Qinghaosu) and the phytochemistry of Artemisia annua L. (Qinghao). Molecules 2010, 15, 7603-7698.

Boyer, J.; Brown, D.; Liu, R. H. Uptake of Quercetin and Quercetin 3-Glucoside from Whole Onion and Apple Peel Extracts by Caco-2 Cell Monolayers. J. Agric. Food Chem.2004, 52, 7172-7179.

Camp, H. S.; Ren, D.; Leff, T. Adipogenesis and fat-cell function in obesity and diabetes. Trends Mol. Med. 2002, 8(9),442-447.

Cohen, G.; Dembiec D.; Marcus J. Measurement of catalase in tissue extracts. Anal. Biochem. 1970, 34, 30-38.

Chang, S. L.; Chiang, Y. M.; Chang, C. L. T.; Yeh, H. H.; Shyur, L. F.; Kuo,Y.H.; Wu, T. K.; Yang,W. C. Flavonoids, centaurein and centaureidin,from Bidens pilosa, stimulate IFN-γ expression. J. Ethnopharmacol. 2007,112(2), 232–236.


Christensen, L.P.; Brandt,K.Bioactive polyacetylenes in food plants of the Apiaceae family:Occurrence, bioactivity and analysis. Journal of Pharmaceutical and Biomedical Analysis. 2006, 41, 683–693.

Chiang, Y. M.; Chang, C. L. T.; Chang, S. L.; Yang, W. C.; Shyur, L. F.Cytopiloyne, a novel polyacetylenic glucoside from Bidens pilosa functions as a T helper cell modulator. J. Ethnopharmacol. 2007, 110, 532–538.

Choi, S. Z.; Lee, S. O.; Choi, S. U.; Lee, K. R. A New Sesquiterpene Hydroperoxide from the Aerial Parts of Aster oharai. Arch. Pharm.Res. 2003, 26 (7), 521-525.

Deba, F.; Xuan, T. D.; Yasuda, M.; Tawata, S. Herbicidal and fungicidal activities and identification of potential phytotoxins from Bidens pilosa L. var. radiata Scherff. Weed Biol. Manag. 2007, 7, 77-83.

Dixon R.A.; Xie D.Y.; Sharma S. B. Proanthocyanidins – a final frontier in flavonoid research? New Phytologist.2005, 165(1), 9-28.

Dimo, T. ; Azay, J. ; Tan, P. V. ; Pellecuer, J.; Cros,G.; Bopelet, M. ; Serrano, J. J. Effects of the aqueous and methylene chloride extracts of Bidens pilosa leaf on fructose-hypertensive rats. J. Ethnopharmacol. 2001, 76(3), 215-221.

Ganjewalaa, D.; Kumara, S.; Ambikaa, K.; Luthrab, R. Plant polyacetylenic glycosides occurrence, biosynthesis and biological activities. Pharmacol. Online. 2008, 2, 113-131.


Gao, J.; Hugger, E.D.; Beck-Westermeyer, M. S.; Borchardt, R.T. Current proprcols in pharmacology. JohnWiley & Sons, Chichester. 2000, 7.2.1-7.2.23.

Geissberger, P.; Séquin, U. Constituents of Bidens pilosa L.: Do the components found so far explain the use of this plant in traditional medicine? Acta Tropica.
1991, 48, 251-261.

Gray, A. M.; Flatt, P. R. Pancreatic and extra- pancreatic effects of the traditional anti-diabetic plant, Medicago sativa (lucerne), Br. J. Nutr. 1997, 78, 325–334.

Green, H.; Meuth, M. An established pre-adipose cell line and its differentiation in culture. Cell. 1974, 3(2), 127-133.

Gregoire, F. M. Adipocyte differentiation: from fibroblast to endocrine cell. Exp.Biol.Med. 2001, 226(11), 997-1002.

Harborne, J.B. PhytochemicalMethods: A Guide toModern Techniques of Plant Analysis, 3rd ed., Chapman & Hall, London, England, 1998.

Hoffmann, B; Hölzl, J.Chalcone glucosides from Bidens pilosa. Phytochemistry 1989, 28, 247-249.

Horiuchi, M.; Seyama, Y. Anti-inflammatory and anti-allergic activity of Bidens pilasa L. var. radiata Scherff. Journal of Health Science.2006, 52 (6), 711–717.


Hsu, C. L.; Huang, S. L.; Yen, G. C. Inhibitory effect of phenolic acids on the proliferation of 3T3-L1 preadipocytes in relation to their antioxidant activity. Agric. Food Chem. 2006, 54, 4191-4197

Hsu, C. L.; Yen, G. C. Effects of flavonoids and phenolic acid on the inhibition of adipgensis in 3T3-L1 adipocytes. J Agric Food Chem. 2007, 55, 8404-8410.

Hsua, Y. J.; Leea, T. H.; Chang, C. L. T.; Huang, Y. T. ; Yanga, W. C. Anti-hyperglycemic effects and mechanism of Bidens pilosa water extract.Journal of Ethnopharmacology. 2009, 122, 379–3830.

Islam, M.S.; Yoshimoto, M.; Yahara, S.; Okuno, S.; Ishiguro, K.; Yamakawa, O. Identification and Characterization of Foliar Polyphenolic Composition in Sweetpotato (Ipomoea batatas L.) Genotypes. J. Agric. Food Chem. 2002, 50, 3718−3722.

Julkunen-Titto. Phenolic constituents in the leaves of northern willows: Methods for the analysis of certain phenolics. Journal of Agricultural and Food Chemistry. 1985, 33, 213–217.

Kalogeropoulos, N.; Yannakopoulou, K.; Gioxari, A.; Chiou, A.; Makris, D. P.Polyphenol characterization and encapsulation in β-cyclodextrin of a flavonoid-rich Hypericum perforatum (St John’s wort) extract. LWT - Food Science and Technology. 2010, 43, 882–889.


Konishi, Y.; Kobayashi, S.; Shimizu, M. Tea polyphenols inhibit the transport of dietary phenolic acid mediated by the moncarboxylic acid transporter (MCT) in intestinal Caco-2 cell monolayer. J.Agric.Food Chem. 2003, 51, 7296-7302.

Konishi, Y.; Shimizu, M. Transepithelial transport of ferulic acid by monocrboxylic acid transporter in Caco-2 cell monolayers. Biosci.Biotechnol.Biochem. 2003, 67, 856-862.

Konishi, Y.; Kobayashi, S.; Shimizu, M. Transepithelial transport of p-coumaric acid and gallic acid in Caco-2 cell monolayers. Biosci. Biotechnol.Biochem. 2003, 67, 2317-2324.

Kumar, J.K.; Sinha, A.K. A new disubstituted acetylacetone from the leaves of Bidens pilosa Linn. Nat. Prod. Res. 2003, 17, 71-74.

Kviecinski, M.R.; Benelli, P.; Felipea,K.B.; Correiaa,J.F.G.; Pichc, C.T.;Ferreirab,S.R.S.; Pedrosaa,R.C. SFE from Bidens pilosa Linné to obtain extracts rich in cytotoxic polyacetylenes with antitumor activity. J. of Supercritical Fluids. 2011, 56, 243–248.

Lima, S. F.; Fischer, D. C. H.; Tavares, J. F.; Silva, M. S.; Athayde-Filho P. F.; Barbosa-Filho J. M. Compilation of Secondary Metabolites from Bidens pilosa L. Molecules. 2011, 16, 1070-1102.


Lin, L. L.; Wu, C. Y.; Hsiu, H. C.; Wang, M. T.; Chuang, H. Diabetes mellitus. I.Hypoglycemic activity of phytosterin on alloxan-diabetic rats. Taiwan Yixuehui Zazhi. 1967, 66, 58-66.

Li, Y.; Shin, Y G.; Yu, C.; Kosmeder, J. W.; Hirschelman, W. H.; Pezzutoand, J. M. Increasing the Throughput and Productivity of Caco-2 Cell Permeability Assays Using Liquid Chromatography-Mass Spectrometry: Application to Resveratrol Absorption and Metabolism. Combinatorial Chemistry & HighThroughput Screening. 2003, 6, 757-767.

Ma, Y.; Ye, X.; Hao, Y.; Xu, G.; Xu, G.; Liu, D. Ultrasound-assisted extraction of hesperidin from Penggan(Citrus reticulata) peel. Ultrasonics Sonochemistry. 2008, 15(2), 227–232.

Madgula, V. L.M.; Avula, B.; Choi, Y. W.; Pullela, S.V.; Khan, I. A.; Walker, L. A.; Khan, S. I. Transport of Schisandra chinensis extract and its biologically-active constituents across Caco-2 cell monolayers in-vitro model of intestinal transport. Journal of Pharmacy and Pharmacology. 2008,60(3), 363-370.

Marklund, S.; Marklund, G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 1974, 47, 469-474.

Melecchi, M. I. S.; Péres, V. F.; Dariva, C.; Zini, C. A.; Abad, F. C.; Martinez, M.M.; Caramão, E. B. Optimization of the sonication extraction method of Hibiscus tiliaceus L. Flowers. Ultrasonics Sonochemistry. 2006, 13(3), 242–250.


Miller, N. J.; Rice-Evans, C.; Davies, M. J.; Gopinathan, V.; Milner, A. A novel method for measuring anti-oxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin. Sci. 1993, 84, 407-412.

Oyaizu, M. Antioxidative activities of browning products of glucosamine fractionated by organic solvent and thin-layer chromatography. Nippon Shokuhin Kogyo Gakkaishi. 1986, 35, 771-775.

Olmos, A.; Guner, R. M.; Recio, M. C.; Rios, J. L.; Gil-Benso, R.; Marnez, S.Interaction of dicaffeoylquinic derivatives with peroxynitrite and other reactive nitrogen species. Arch. Biochem. Biophys. 2008, 475, 66-71.

Palma, M.; Barroso, C.G. Ultrasound-assisted extraction and determination of tartaric and malic acids from grapes and winemaking by-products. Analytica Chimica Acta. 2002, 458, 119–130.

Prior, R. L.; Gu, L. Occurrence and biological significance of proanthocyanidins in the American diet. Phytochemistry. 2005, 66, 2264–2280.

Prabhakar, P. K.; Doble, M. Synergistic effect of phytochemicals in combination with hypoglycemic drugs on glucose uptake in myotubes. Phytomedicine. 2009, 16, 1119-1126.

Rabe, T.; Johannes van Staden. Antibacterial activity of South African plants used for medicinal purposes. J. Ethnopharmacol. 1997, 56 (1), 81-87.

Sarg, T.M.; Ateya, A.M.; Farrag, N.M.; Abbas, F.A. Constituents and biological activity of Bidens pilosa L. grown in Egypt. Acta Pharm. 1991, 61, 317-323.


Scalbert, A.; Williamson, G. Dietary intake and bioavailability of polyphenol. J.Nutr. 2000, 130, 2073-2085.

Shimada, K.; Fujikawa, K.; Yahara, K.; Nakamura, T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J. Agric. Food Chem. 1992, 40, 945-948.

Sivakumar V.; Verma, V. R.; Rao, P.G.; Swaminathan, G. Studies on the use of power ultrasound in solideliquid myrobalan extraction process. Journal of Cleaner Production. 2007, 15, 1813-1818.

Sundararajan, P.; Dey, A.; Smith, A.; Doss, A. G.; Rajappan, M.; Natarajan, S. Studies of anticancer and antipyretic activity of Bidens pilosa whole plant. African Health Sciences. 2006, 6 (1), 27-30.

Suzigan, M. I.; Battochio, P. R.; Coelho, K. L. R.; Coelho, C. A. R. An acqueous extract of Bidens pilosa L. protects liver from cholestatic disease Experimental study in young rats. Acta Cirúrgica Brasileira. 2009, 24 (5), 347-352.

Taga, M.S.; Miller, E.E.; Partt, D.E. Chin seeds as a source of natural lipid antioxidants. J. Am.Oil. Chem. Soc. 1984, 61, 928-931.

Tan, P. V.; Dimo, T.; Dongo, E. Effects of methanol, cyclohexane and methylene Chloride extracts of Bidens pilosa on various gastric ulcer models in rats. J. Ethnopharmacol. 2000, 73(3), 415-421.

Toma, M.; Vinatoru, M.; Paniwnyk, L.; Mason, T. J. Investigation of the effects of ultrasound on vegetal tissues during solvent extraction. Ultrasonics Sonochemistry. 2001, 8(2), 137–142.


Ubillas, R.P.; Mendez, C.D.; Jolad, S.D.; Luo, J.; King, S.R.; Carlson, T.J.; FoD.M. Antihyperglycemic acetylenic glucosides from Bidens pilosa. Planta Medica. 2000, 66, 82–83.

Valachovic, P.; Pechova, A.; Mason, T. J. Towards the industrial production of medicinal tincture by ultrasound assisted extraction. Ultrasonics Sonochemistry. 2001, 8(2), 111–117.

Wat, C. K.; Johns, T.; Towers, G. H. N. Phototoxic and antibiotic activities of plants of the Asteraceae used in folk medicine. J. Ethnopharmacol. 1980,279-290.

Walle U. K.; Galijatovic, A.; Walle, T.. Transport of the flavonoid chrysin and conjugated metabolites by human intestinal cell line Caco-2. Biochemical Pharmacology. 1999, 58, 431-438.

Waterhouse D. F. Biological Control of Weeds: Southeast Asian Prospects. 1994, ISBN: 1-86320-099-1

Walgren, R. A.; Lin, J. T.; Kinne, R. K. H.; Walle, T. Cellular uptake of dietary flavonoid quercetin 4′-β-gluocside by sodium-dependent glucose transport SGL1. J.Pharmacol. Exp. Ther. 2000, 294, 837-843.

Yang, C. S.; Landau, J. M.; Huang, M. T.; Newmark, H. L. Inhibition of Carcinogenesis by dietary polyphenolic compounds. Annu. Rev.Nutr.2001, 21, 381-406.


Yang, H. L.; Chen, S. C.; Chang, N. W.; Chang, J. M.; Lee, M. L.; Tsai, P. C.H. H.; Kao, W. W.; Chiang, H. C.; Wang, H. H.; Hseu, Y. C. Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocy Food and Chemical Toxicology. 2006, 44 (9), 1513–1521.

Yuan, L. P.; Chen, F. H.; Ling, L.; Dou, P. F.; Bo, H.; Zhong, M. M.; Xia, L. Protective effects of total flavonoids of Bidens pilosa L. (TFB) on anima injury and liver fibrosis. J. Ethnopharmacol. 2008, 116 (3), 539-546.

Zhao, S.; Kwok, K.C.; Liang, H. 2007. Investigation on ultrasound assisted extraction of saikosaponins from Radix Bupleuri. Separation and Purification Technology. 2007, 55, 307–312.

Zhishen, J.; Mengcheng, T.; Jianming, W. Research on antioxidant activity of flavonoids from natural materials. Food Chem. 1999, 64, 555- 559.

Zulueta, M.C.A.; Tada, M.; Ragasa, C.Y. A diterpene from Bidens pilosa. Phytochemistry. 1995, 38, 1449-1450.

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