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研究生:劉銘茹
論文名稱:當歸與紫草化學分析與品種比較
論文名稱(外文):Chemical analysis and compare of kind in Angelica sinensis (Oliv.) Diels and Lithospermum erythrorhizon.
指導教授:黃文盈
學位類別:碩士
校院名稱:弘光科技大學
系所名稱:化妝品科技研究所
學門:民生學門
學類:美容學類
論文種類:學術論文
論文出版年:2011
畢業學年度:99
語文別:中文
論文頁數:115
中文關鍵詞:當歸紫草化學分析品種比較
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高效液相層析儀(HPLC)及質譜儀(MS)為目前常用來定性定量中藥成份的方法,而本研究的目地在於開發HPLC之分析方法,尋找適合條件,以分析當歸及紫草之成份含量,並比較該兩種藥材之優劣。同時,利用分析的結果,進行當歸及紫草之基原化學鑑定。

當歸及紫草皆為常用中藥材,當歸為補血、活血、鎮靜、消炎、調經之功能,紫草為活血、消炎、調經之藥理作用。其中當歸分析出12種成分,分別為Protocatechuic acid (PCA)、Phthalic acid (PHA)、Vanillic acid (VA)、Ferulic acid (FA)、n-Butylidenephthalide (n-BLP)、Senkyunolide F (SK-F)、3-Butylidene-4-hydroxyphthalide (BLHP)、Butylphthalide (BP)、Z-Ligustilide (Z-LS)、Coniferyl ferulate (CFF)、Angelicide (AL)、Levistolide A (LT-A)。而紫草為分析出9種成份,分別為Ethylshikonin (ESK)、2-methyl-n-butyrylshikonin (MBSK)、Shikonin (SK)、3-hydroxy-isovalerylshikonin (HIVSK)、Acetylshikonin (ASK)、β-acetoxyisovalerylshikonin (AIVSK)、Isobutylshikonin (IBSK)、β, β-dimethylacrylshikonin (DASK)、Isovalerylshikonin (IVSK)。

在HPLC分析條件中,當歸之最佳分析條件為ACN/H2O= 4/1、10 mM KH2PO4、pH 3.62、5C18-AR-II,分析時間82分鐘。紫草之最佳分析條件為ACN/H2O= 4/1、15 mM KH2PO4、pH 3.33、5C18-AR-II,分析時間為30分鐘。在分析時間、偵測極限及再現性進行比較下,達到較快速並準確之效果。

同時於台灣各地收集19批當歸樣品及18批紫草樣品,其中亦包含正品及市售藥材。故以此套分析方法快速分析定量,並以其成份之比值進一步比較,以達到區分出正品及市售藥材,在此方法衍生下,期待可辨別出藥材之優劣,並在品質上進行管理。

HPLC are the most commonly used methods for analyzing Chinese herb drug components. This study is aimed to develop HPLC methods with the most-optimized conditions for analyzing the content of Angelica Radix and Lithospermum erythrorhizon and to compare the advantage as well as drawback of both Chinese herb drug.

Angelica Radix and Lithospermum erythrorhizon are commonly used Chinese herb drug. Both have blood hemopoietic, blood enlivening, tranquilizing, reduce inflammation and menstrual regulating effects. Analysis 12 components of Angelica Radix, include Protocatechuic acid (PCA), Phthalic acid (PHA), Vanillic acid (VA), Ferulic acid (FA), n-Butylidenephthalide (n-BLP), Senkyunolide F (SK-F), 3-Butylidene-4-hydroxyphthalide (BLHP), Butylphthalide (BP), Z-Ligustilide (Z-LS), Coniferyl ferulate (CFF), Angelicide (AL) and Levistolide A (LT-A). Analysis 9 components of Lithospermum erythrorhizon, include Ethylshikonin (ESK), 2-methyl-n-butyrylshikonin (MBSK), Shikonin (SK), 3-hydroxy-isovalerylshikonin (HIVSK), Acetylshikonin (ASK), β-acetoxyisovalerylshikonin (AIVSK), Isobutylshikonin (IBSK), β, β-dimethylacrylshikonin (DASK) and Isovalerylshikonin (IVSK).

In HPLC method, ACN/H2O= 4/1, 10 mM KH2PO4, pH 3.62, 5C18-AR-II, analysis time within 82 mins of optimized analysis conditions of Angelica Radix. ACN/H2O= 4/1, 15 mM KH2PO4, pH 3.33, 5C18-AR-II, analysis time within 30 mins of optimized analysis conditions of Lithospermum erythrorhizon. Comparing the analysis time、limit of detection and reproducibility results of this method to attain to fast and validated.

From Taiwan, we collected 19 sample of Angelica Radix and 18 sample Lithospermum erythrorhizon, which include pure Chinese herb drug and market Chinese herb drug. Proposing a fast and validated method for routine analysis and quality control. Another compare of use components ratio, come to separate from pure Chinese herb drug and market Chinese herb drug.



誌謝
中文摘要
英文摘要
總目錄………………………………………………………………………Ⅰ
表目錄………………………………………………………………………Ⅴ
圖目錄………………………………………………………………………Ⅶ
第一章 緒論
第一節 前言 ………………………………………………………… 1
第二節 高效能液相層析………………………………………………2
第三節 分析條件參數及適宜性之評估……………………………4
1-3-1 分析條件參數………………………………………………4
1-3-2 分析條件之適宜性…………………………………………5
第二章 化學成份之分析 …………………………………………6
第一節 前言 ………………………………………………………… 6
2-1-1 紫草藥材簡介………………………………………………6
2-1-2 當歸藥材簡介………………………………………………7
第二節 化學成份分析……………………………………………… 7
2-2-1 藥材化學成份………………………………………………7
2-2-1-1 紫草化學成份…………………………………… 7
2-2-1-2 當歸化學成份…………………………………… 9
2-2-2 藥材分析與藥理作用 …………………………………… 11
2-2-2-1 紫草分析與藥理作用……………………………11
2-2-2-2 當歸分析與藥理作用……………………………13
2-2-3 實驗部份……………………………………………………18
2-2-3-1 實驗藥品…………………………………………18
2-2-3-2 實驗儀器…………………………………………19
2-2-4 藥材之最佳分析條件………………………………………19
2-2-4-1 紫草之分析條件………………………………… 19
2-2-4-2 當歸之分析條件………………………………… 22
2-2-5 結果與討論…………………………………………………25
2-2-5-1 紫草分析條件之選擇…………………………… 25
2-2-5-2 當歸分析條件之選擇…………………………… 41
第三節 藥材化學成份之液相層析質譜(LC-MS)分析………58
2-3-1 前言…………………………………………………………56
2-3-2 實驗部份……………………………………………………57
2-3-2-1 實驗藥品………………………………………… 57
2-3-2-2 實驗儀器………………………………………… 57
2-3-3 最佳分析條件………………………………………………58
2-3-4 成份結構質譜分析…………………………………………58
2-3-4-1 紫草之成份質譜分析 ……………………………58
2-3-4-2 當歸之成份質譜分析 ……………………………62
第三章 基原化學鑑定…………………………………………… 66
第一節 前言………………………………………………………… 66
3-1-1 紫草之本草考察……………………………………………66
3-1-2 當歸之本草考察……………………………………………66
3-1-3 藥材品種及產地之簡介……………………………………67
3-1-3-1 紫草品種及產地…………………………………67
3-1-3-2 當歸品種及產地…………………………………68
3-1-4 藥材植物之型態鑑定………………………………………68
3-1-4-1 紫草植物之型態………………………………… 68
3-1-4-2 當歸植物之型態………………………………… 69

3-1-5 藥材性狀分析………………………………………………70
3-1-5-1 紫草性狀分析……………………………………70
3-1-5-2 當歸性狀分析……………………………………71
3-1-6 藥材組織顯微鑑定…………………………………………72
3-1-6-1 紫草組織顯微鑑定………………………………72
3-1-6-2 當歸組織顯微鑑定………………………………74
第二節 實驗部份……………………………………………………75
3-2-1 藥品與儀器…………………………………………………75
3-2-1-1紫草藥品………………………………………… 75
3-2-1-2 當歸藥品…………………………………………79
3-2-2 實驗條件……………………………………………………81
3-2-2-1 紫草之實驗條件………………………………… 81
3-2-2-2 當歸之實驗條件………………………………… 81
3-2-3 樣品的配製…………………………………………………81
3-2-3-1 紫草樣品之配製………………………………… 81
3-2-3-2 當歸樣品之配製………………………………… 81
第三節 結果與討論 ……………………………………………… 81
3-3-1 紫草…………………………………………………………81
3-3-1-1組織鏡檢 ………………………………………… 81
3-3-1-2 HPLC分析圖形的比較……………………………82
3-3-1-3 定量結果 …………………………………………83
3-3-1-4 品種比較 …………………………………………96
3-3-2 當歸…………………………………………………………99
3-3-2-1組織鏡檢 ………………………………………… 99
3-3-2-2 HPLC分析圖形的比較……………………………99
3-3-2-3 定量結果…………………………………………100
3-3-2-4 品種比較…………………………………………109
第四章 結論…………………………………………………………112
參考文獻…………………………………………………………………113


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