臺灣博碩士論文加值系統

鳳尾草為傳統中草藥，具有抗氧化、消炎、抑菌、抗癌、抗腫瘤、抗病毒等功效。近年來，許多研究學者對各方面的研究工作不斷深入，但大都採用傳統方式進行精製萃取，目前並無相關的文獻報導利用超臨界二氧化碳的方式進行萃取其有效成分。本研究探討萃取條件範圍在:壓力300 bar、400 bar、500 bar，溫度313 K、323 K、333 K，二氧化碳流量2L/min、3L/min和添加乙醇助溶劑流量0.1mL/min、0.2mL/min、0.3mL/min對鳳尾草萃取量之影響。操作最佳萃取條件在壓力500 bar、溫度333 K和流量3L/min，並採用反相高效液相色譜儀器檢測，由超臨界二氧化碳萃取鳳尾草中的成分分析，以甲醇溶解鳳尾草萃取的樣品，然後以MetaChem C18-A(250×4.6mm，5μm)層析管柱為固定相，1％醋酸水溶液和乙腈(70/30，v/v)為流動相、檢測波長255nm、流速設定在1mL/min等條件，在該色譜條件下，鳳尾草中木犀草素成分波峰滯留時間在9.5分鐘左右，並與木犀草素標準品比較對照。木犀草素最佳萃取條件為壓力500 bar、溫度333 K和流量3L/min。

Pteris multifida Poiret is a traditional herbal medicine.It was used as antioxidant, Anti-inflammatory, antibacterial, anticancer and antitumor for a long time. Recently, it was mostly extracted by traditional method. No related report has been published for the utilization of supercritical carbon dioxide to extract active components of Pteris multifida Poiret. This study investigates the extraction of the Pteris multifida Poiret under the following conditions: pressure at 300 bar, 400 bar, 500 bar, temperatures at 313 K, 323 K, 333 K, CO2 flow rate at 2,3L/min and co-solvent ethanol flow rate at 0.1mL/min, 0.2mL/min, 0.3mL/min impact on the extract amount of Pteris multifida Poiret. The best operation of extraction is under 500 bar, 333 K, and 3L/min CO2 flow rate. The reverse-phase high performance liquid chromatography method was developed for determing major active components of Pteris multifida Poiret by supercritical carbon dioxide extraction. We extracted the sample with methanol and analyzed by using a MetaChem C18-A(250×4.6mm,5μm) column with a gradient elution composed of 1％ acetic acid aqueous solution and acetonitrile(70/30，v/v). The wavelength of UV was set at 255nm and the flow-rate was set at 1mL/min. Under this condition, the peaks of the Luteolin was identified by comparing the retention time with that of the standard reagent. Typical retention time of Luteolin was 9.5min. The best operation of extraction Luteolin is in pressure 500 bar, temperature 333 K, and CO2 flow rate kept at 3L/min.

明志科技大學碩士學位論文指導教授推薦書.....................................................i
明志科技大學碩士學位論文口試委員審定書....................................................ii
明志科技大學學位論文授權書...........................................................................iii
誌謝.......................................................................................................................iv
中文摘要................................................................................................................v
Abstract..................................................................................................................vi
目錄......................................................................................................................vii
表目錄...................................................................................................................ix
圖目錄....................................................................................................................x
第一章 緒論.........................................................................................................1
1-1 前言...........................................................................................................1
1-2 研究動機...................................................................................................3
第二章 文獻回顧.................................................................................................4
2-1 鳳尾草簡介...............................................................................................4
2-2 黃酮類簡介...............................................................................................5
2-3 超臨界流體應用簡介...............................................................................7
2-4 超臨界流體性質.....................................................................................13
2-5 超臨界萃取裝置簡介.............................................................................14
2-6 超臨界二氧化碳萃取法簡介.................................................................16
第三章 實驗材料與方法...................................................................................20
3-1 實驗材料.................................................................................................20
3-2 實驗設備.................................................................................................21
3-3 實驗方法.................................................................................................22
3-3-1 超臨界萃取實驗步驟..................................................................22
3-3-2 高效能液相層析儀分析..............................................................23
第四章 結果與討論...........................................................................................25
4-1 超臨界二氧化碳萃取部份......................................................................25
4-1-1 烘乾時間對萃取率影響..............................................................25
4-1-2 於固定壓力、流量下，溫度對萃取量之影響..........................26
4-1-3 於固定溫度、流量下，壓力對萃取量之影響..........................26
4-1-4 於固定壓力、溫度下，流量對萃取量之影響..........................27
4-1-5 於固定壓力、溫度、流量下，助溶劑對萃取量之影響.….....28
4-1-6 不同萃取時間對萃取量之影響..................................................28
4-2 高效能液相層析法測定鳳尾草的成分.................................................29
第五章 結論.......................................................................................................31
參考文獻...............................................................................................................32

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